Matching Items (844)
Description
Laboratory animals represent an invaluable, yet controversial, resource in the field of biomedical research. Animal research has been behind many influential discoveries in the field of emerging therapeutics. They provide the link between the theory of the lab bench and the functional application of medicine to influence human health. The use of animals in research is a consideration which must be heavily weighed, and the implementation must be carried out at a very high standard in order to retain research integrity and responsibility. We are in the process of conducting an experiment using laboratory mice to demonstrate cancer treatment using vaccinia (VACV) mutants as a possible oncolytic therapy for certain strains of melanoma. VACV is a double-stranded DNA poxvirus with a large and easily altered genome. This virus contains many genes dedicated to immune evasion, but has shown sensitivity to cell death by necroptosis in mouse studies (5). We have identified the absence of the kinase RIP3 which is vital in the necroptosis pathway as a potential target for oncolytic therapy using VACV mutants in specific strains of melanoma. Multiple groups of SCID Beige mice were inoculated with different melanoma cell lines and observed for tumor growth. Upon reaching 1 cm3 in volume, tumors were injected with either VACV- Δ83N, VACV- Δ54N, or PBS, and observed for regression. It was hypothesized that melanoma tumors that are RIP3-/- such as the MDA5 cell line will show regression, but melanoma tumors that are RIP3-positive and capable of necroptosis, such as the 2427 cell line, will resist viral replication and continue to proliferate. Our results so far tentatively support this hypothesis, but the data collection is ongoing. Strict and specific protocols with regard to the ethical and responsible use of mice have been implemented and upheld throughout the experiment. Animals are closely monitored, and if their quality of life becomes too poor to justify their continued use in the experiment, they are humanely euthanized, even at the expense of valuable data. The importance of commitment to a high ethical standard is pervasive throughout our work. Animals represent an invaluable contribution to research, and it is important to maintain high standards and transparency with regard to their use. Education and engagement in critical discussions about the use and care of animals in the laboratory contribute to the overall merit and legitimacy of biomedical research in the public and professional eye as a whole, and give legitimacy to the continued use of animals as models to advance science and health.
ContributorsBergamaschi, Julia (Author) / Kibler, Karen (Thesis director) / Jacobs, Bertram (Committee member) / School of Human Evolution and Social Change (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
For this project, I use qualitative textual analysis to compare the differences and/or similarities between (1) how the former residents of Tempe’s historic San Pablo barrio (1872-1955) conveyed their sense of place, meaning, and displacement in oral and written histories and (2) how Tempe’s Anglo residents at the time of San Pablo’s occupation and dissolution conveyed their sense of the place, meaning, and displacement of San Pablo in newspaper articles. I have located my investigation of any perceived or lacking disparities between how these two groups perceived San Pablo’s place and meaning within the context of San Pablo’s dissolution and the displacement of its residents in the mid 1950s. As I follow the process through which some communities are able to suppress, take over, and erase others from dominant narratives and political decisions without any perceived consequences, I will bring to the foreground the emotional impact of place and displacement in order to highlight how the former residents of ‘erased’ communities make sense of and respond to their displacement.
ContributorsRoberson, Jessica Bridges (Author) / Michelaki, Konstantina-Eleni (Thesis director) / Toon, Richard (Committee member) / School of Human Evolution and Social Change (Contributor) / School of Social Transformation (Contributor) / Department of English (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
The lonely, empty face panhandling on the street corner has a story to tell. At first glance, most people write the homeless off as less than worthy. However, there is a deeper connection and understanding of relationships and a sense of community unseen by the majority of passer-byers. Amidst the humdrum beat of every day life, there is toil to find basic necessities such as food, water, sanitation, and a place to rest. At the same time, there is laughter and friendship as they help one another through the hostilities of their circumstances. Combining the creative elements of photojournalism and qualitative interviewing, the basic daily needs and struggles of the homeless will be delved into to answer how friendship is pertinent to survival on the streets.
ContributorsFisher, Meghan (Author) / Hruschka, Daniel (Thesis director) / Hita, Liza (Committee member) / Newland, Judy (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
Examining the effect of various factors such as class, gender, and status on health inequalities in India is crucial for improving access to health services. However, most research on castes in India is done using broad, government-defined categories, including “Scheduled Castes,” “Scheduled Tribes,” “Forward Classes,” and “Other Backwards Classes.” These general categories erase the experiences that more specific caste groups have in their health and their livelihood. Using the 2015-2016 Demographic and Health Survey of India, this study analyzes data on 699,686 women aged 15-49 to investigate the association of socioeconomic status, assessed by wealth, education, and community status on two types of contraceptive use–sterilization and other modern methods. Those with secondary education or higher were much less likely to be sterilized and more likely to use non-sterilization forms of modern contraception. Interestingly, those with greater household wealth were more likely to be sterilized but had no different chance of using non-sterilization forms of modern contraception. After controlling for other socioeconomic factors, members of Scheduled Castes and Backward Classes are more likely to be sterilized. However, there was additional heterogeneity between groups, with Muslim groups and Northeastern tribes much less likely to be sterilized, and Southern tribes more likely to be sterilized. Moreover, Muslim groups as well as Northern and Northeastern tribes were more likely to use non-sterilizations forms of contraception, whereas Southern tribes were less likely to use non-sterilization forms of contraception. These findings illustrate that in addition to differences by major caste categories, there is still religious and regional variation in the likelihood of using different forms of contraception. Future research should examine how unique community factors erased within the already present government caste system can affect health. In addition, more research should be done on the various effects of discrimination faced by these communities and how this discrimination affects their health education, access, and autonomy.
ContributorsChandra, Natasha (Author) / Hruschka, Daniel (Thesis director) / Drake, Alexandria (Committee member) / Barrett, The Honors College (Contributor) / School of Human Evolution & Social Change (Contributor) / School of International Letters and Cultures (Contributor) / Dean, W.P. Carey School of Business (Contributor)
Created2024-05