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- Creators: Ira A. Fulton Schools of Engineering
- Creators: Department of Supply Chain Management
- Resource Type: Text
- Status: Published
Description
Cellular heterogeneity is a key factor in various cellular processes as well as in disease development, especially associated with immune response and cancer progression. Cell-to-cell variability is considered to be one of the major obstacles in early detection and successful treatment of cancer. Most present technologies are based on bulk cell analysis, which results in averaging out the results acquired from a group of cells and hence missing important information about individual cells and their behavior. Understanding the cellular behavior at the single-cell level can help in obtaining a complete profile of the cell and to get a more in-depth knowledge of cellular processes. For example, measuring transmembrane fluxes oxygen can provide a direct readout of the cell metabolism.
The goal of this thesis is to design, optimize and implement a device that can measure the oxygen consumption rate (OCR) of live single cells. A microfluidic device has been designed with the ability to rapidly seal and unseal microchambers containing individual cells and an extracellular optical oxygen sensor for measuring the OCR of live single cells. The device consists of two parts, one with the sensor in microwells (top half) and the other with channels and cells trapped in Pachinko-type micro-traps (bottom half). When the two parts of the device are placed together the wells enclose each cell. Oil is flown in through the channels of the device to produce isolated and sealed microchamber around each cell. Different fluids can be flowed in and out of the device, alternating with oil, to rapidly switch between sealed and unsealed microenvironment around each cell. A fluorescent ratiometric dual pH and oxygen sensor is placed in each well. The thesis focuses on measuring changes in the oxygen consumption rate of each cell within a well. Live and dead cells are identified using a fluorescent live/dead cell assay. Finally, the technology is designed to be scalable for high-throughput applications by controlling the flow rate of the system and increasing the cell array density.
The goal of this thesis is to design, optimize and implement a device that can measure the oxygen consumption rate (OCR) of live single cells. A microfluidic device has been designed with the ability to rapidly seal and unseal microchambers containing individual cells and an extracellular optical oxygen sensor for measuring the OCR of live single cells. The device consists of two parts, one with the sensor in microwells (top half) and the other with channels and cells trapped in Pachinko-type micro-traps (bottom half). When the two parts of the device are placed together the wells enclose each cell. Oil is flown in through the channels of the device to produce isolated and sealed microchamber around each cell. Different fluids can be flowed in and out of the device, alternating with oil, to rapidly switch between sealed and unsealed microenvironment around each cell. A fluorescent ratiometric dual pH and oxygen sensor is placed in each well. The thesis focuses on measuring changes in the oxygen consumption rate of each cell within a well. Live and dead cells are identified using a fluorescent live/dead cell assay. Finally, the technology is designed to be scalable for high-throughput applications by controlling the flow rate of the system and increasing the cell array density.
ContributorsRodrigues, Meryl (Author) / Meldrum, Deirdre (Thesis advisor) / Kelbauskas, Laimonas (Committee member) / LaBelle, Jeffrey (Committee member) / Arizona State University (Publisher)
Created2014
Description
Single cell analysis has become increasingly important in understanding disease onset, progression, treatment and prognosis, especially when applied to cancer where cellular responses are highly heterogeneous. Through the advent of single cell computerized tomography (Cell-CT), researchers and clinicians now have the ability to obtain high resolution three-dimensional (3D) reconstructions of single cells. Yet to date, no live-cell compatible version of the technology exists. In this thesis, a microfluidic chip with the ability to rotate live single cells in hydrodynamic microvortices about an axis parallel to the optical focal plane has been demonstrated. The chip utilizes a novel 3D microchamber design arranged beneath a main channel creating flow detachment into the chamber, producing recirculating flow conditions. Single cells are flowed through the main channel, held in the center of the microvortex by an optical trap, and rotated by the forces induced by the recirculating fluid flow. Computational fluid dynamics (CFD) was employed to optimize the geometry of the microchamber. Two methods for the fabrication of the 3D microchamber were devised: anisotropic etching of silicon and backside diffuser photolithography (BDPL). First, the optimization of the silicon etching conditions was demonstrated through design of experiment (DOE). In addition, a non-conventional method of soft-lithography was demonstrated which incorporates the use of two positive molds, one of the main channel and the other of the microchambers, compressed together during replication to produce a single ultra-thin (<200 µm) negative used for device assembly. Second, methods for using thick negative photoresists such as SU-8 with BDPL have been developed which include a new simple and effective method for promoting the adhesion of SU-8 to glass. An assembly method that bonds two individual ultra-thin (<100 µm) replications of the channel and the microfeatures has also been demonstrated. Finally, a pressure driven pumping system with nanoliter per minute flow rate regulation, sub-second response times, and < 3% flow variability has been designed and characterized. The fabrication and assembly of this device is inexpensive and utilizes simple variants of conventional microfluidic fabrication techniques, making it easily accessible to the single cell analysis community.
ContributorsMyers, Jakrey R (Author) / Meldrum, Deirdre (Thesis advisor) / Johnson, Roger (Committee member) / Frakes, David (Committee member) / Arizona State University (Publisher)
Created2012
Description
The purpose of this paper is to understand how companies are finding high potential employees and if they are leaving top talent behind in their approach. Eugene Burke stated in 2014 that 55% of employees that are labeled as a High Potential Employee will turn over and move companies. Burke (2014) also states that the average high potential employee tenure is five years. The Corporate Leadership Council says that on average, 27% of a company's development budget is spent on its high potential program (CEB 2017). For a midsize company, the high potential development budget is almost a million dollars for only a handful of employees, only to see half of the investment walking out the door to another company . Furthermore, the Corporate Leadership Council said that a study done in 2005 revealed that 50% of high potential employees had significant problems within their job (Kotlyar and Karkowsky 2014). Are time and resources are being given to the wrong employees and the right employees are being overlooked? This paper exams how companies traditionally select high potential employees and where companies are potentially omitting employees who would be better suited for the program. This paper proposes that how a company discovers their top talent will correlate to the number of turnovers or struggles that a high potential employee has on their job. Future research direction and practical considerations are also presented in this paper.
ContributorsHarrison, Carrie (Author) / Mizzi, Philip (Thesis director) / Ruediger, Stefan (Committee member) / Department of Management and Entrepreneurship (Contributor) / School of Sustainability (Contributor) / Department of Supply Chain Management (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
In which industry that has ever been profit generating, does a firm profit from their failure? The United States has a mass incarceration problem. With 25% of the world prison population residing in the US, spending on detention costs the US government $80 billion annually. Over 50% of the individuals incarcerated in America are of black or Latino descent. This massive growth in the incarcerated population of America began in the 1970s and with the passive support of American citizens has created an industry whose players profit from the detention of people. Currently, the privately run detention facilities in the United States hold 7% of state prisoners, 18% of federal prisoners, and nearly 75% of ICE's undocumented detainee population. The detention of people for profit is an idea rooted in the same profit motive that allowed the institution of slavery to flourish. However even after the 13th Amendment abolished slavery in the U.S., the oppressive forces behind slave-era economics have been perpetuated through legislation and policies that continued the stratification of society and reinforcement of the social order. With the help of corporate lobbyists, political action committees, and organizations such as the American Legislative Exchange Council, the corporate shareholders of private prisons, such as CoreCivic and The GEO Group, are able to directly align their profit-driven interests with those of federal and state legislators. By the incorporation of legislation and policy into state and federal law, the shareholders of private prisons are able to directly affect legislation as well as their own potential for profit. The justification for the usage of private prisons is thought to be seen in the price savings and flexibility that it provides for federal and state governments. However, due to the law enforcement contractor's exemption from public record laws, there is no clear evidence of where the cost savings occur, or even if there are cost savings at all. Is it ethical for a for-profit-prison corporation to be responsible for the care, security, and rehabilitation of an individual, when if they fail to rehabilitate the individual, it will add to the number of inmates under their control? The measure of a prison's failure to rehabilitate an inmate is considered the recidivism rate, and is affected when an inmate leaves a detention facility, commits another crime, then is arrested. This profit motive is causing our society to incarcerate increasing numbers of people in private prisons. For-profit prisons financially benefit from long-term incarceration and recidivism. The passive investments from public and private employees and institutions through investment corporations are the legs that allow the private prison industry to stand. Twenty-nine investment firms, such as The Vanguard Group and Fidelity Investments, own nearly two-thirds of the two largest players in the private prison industry. This includes the passive investments by public institutions such as the Arizona State University Foundation's $600 million endowment fund as well as the $500 million directly invested into CoreCivic and GEO Group from the University of Texas/ Texas A&M Investment Management Company. The goal of abolishing private prisons will require years of litigation against the giants of the industry as well as the governmental entities supporting them. However, we can start today by demanding divestiture by our school and similar institutions as well continuing to share the knowledge of the oppressive forces associated with the detention of individuals for profit.
ContributorsBayham, Michael (Author) / Gomez, Alan (Thesis director) / Dacey, John (Committee member) / W.P. Carey School of Business (Contributor) / Department of Supply Chain Management (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
The purpose of this thesis is to explore how Blockchain technology can help solve problems large corporations commonly face. For example, it is a common problem for large businesses and organizations to manage sales contracts with thousands of items on them. Likewise, it can be difficult to accurately monitor complex payment histories with thousands of items on them. Another issue is the difficulty that is introduced when making periodic reconciliations based on separate recording systems. At a broader level, some organizations may hesitate to do business with new strange companies or oversea companies for the first time because they do not trust that the other organization can deliver what they promise. Such problems cost organizations a lot of money, effort, and time to solve. However, Blockchain technology, first developed in 2009, could revolutionize how the business community deals with these common problems. The shared and immutable ledger on Blockchain can help organizations to keep track on transactions, manage the contracts in a smarter way, ensure correct purchase history records, eliminate the periodically reconciliation processes, and provide visibility for real-time transactions.
ContributorsHuynh, Phu Thanh (Author) / Popova, Laura (Thesis director) / Pankaj, Sneha (Committee member) / Department of Supply Chain Management (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
As climate change and air pollution continue to plague the world today, committed citizens are doing their part to minimize their environmental impact. However, financial limitations have hindered a majority of individuals from adopting clean, renewable energy such as rooftop photovoltaic solar systems. England Sustainability Consulting plans to reverse this limitation and increase affordability for residents across Northern California to install solar panel systems for their energy needs. The purpose of this proposal is to showcase a new approach to procuring solar panel system components while offering the same products needed by each customer. We will examine market data to further prove the feasibility of this business approach while remaining profitable and spread our company's vision across all of Northern California.
ContributorsEngland, Kaysey (Author) / Dooley, Kevin (Thesis director) / Keahey, Jennifer (Committee member) / Department of Supply Chain Management (Contributor) / School of Social and Behavioral Sciences (Contributor) / W.P. Carey School of Business (Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
The National Basketball Association (NBA) is one of the Big Four Sporting Leagues of US Professional Sports. In recent years, the NBA has enjoyed milestone seasons in both attendance and television ratings, resulting in steady increases to both, over the previous decade. (Morgan, 2017) This surge can be attributed in part to the integration of "cultural recognition" initiatives and the overall message of inclusivity on the part of NBA franchises, with their respective promotions and advertisements such as television, social media, radio, etc. Heritage Nights, such as "Noche Latina," among other variants in the NBA, typically feature culturally influenced changes to team logos, giveaways, and other consumer offerings. In markets where Hispanics make up a significant percentage of the fan-base, such as Phoenix, NBA franchises such as the Phoenix Suns must ascertain the financial or perceptual impacts, associated with risks of stereotyping, offending or otherwise unintentionally alienating different categories of fans. To this end, data was collected from the local NBA franchises' fanbase, specifically Phoenix Suns season-ticket holders, and was statistically checked for significant relationships between both categories of fans and several different variables. This analysis found that only $192K in revenue is being missed through the investment of Heritage Nights, and that fan perceptions of stereotypical or offensive giveaways and practices have no significant effect on game or event attendance, despite the stereotypes toward giveaways and practices still being present. Implications of this study provide possible next steps for the Suns and continue to widen the scope of demographical sports marketing both in professional basketball and beyond.
ContributorsGibbens, Patrick Alexander (Author) / Eaton, John (Thesis director) / McIntosh, Daniel (Committee member) / Department of Supply Chain Management (Contributor) / School of Music (Contributor) / Department of Marketing (Contributor) / W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Sagebrush Coffee is a small business in Chandler, Arizona that purchases green beans, roasts them in small batches for quality, and ships fresh, gourmet roasted coffee beans across the nation. Deciding which coffee beans to buy and roast is one of the most crucial business decisions Sagebrush and other gourmet coffee roasters face. Further complicating this decision is the fact that coffee is a crop, and like all crops, has a specific growing season and the exact same product cannot usually be ordered from year to year, even if it proves to be successful. The goal of this research is to use data analytics and visualization to help Sagebrush make better purchasing decisions by identifying consumer purchasing trends and providing a recommendation for their portfolio mix. In the end, I found that Latin American coffees are popular with both returning and first-time customers, but a specific country of origin does not appear to be associated with the top coffee producing countries. Additionally, December is a critical month for Sagebrush and Sagebrush should make sure to target the states with the most sales: California, Pennsylvania, and New York. Arizona has growth potential as it is not one of the top three locations, despite the presence of a physical store. Also included in the following report is a portfolio recommendation suggesting how many of each product based on region, processing type, and roast level to carry in inventory.
ContributorsBlue, Jessica Morgan (Author) / Kellso, James (Thesis director) / Davila, Eddie (Committee member) / Department of Information Systems (Contributor) / Economics Program in CLAS (Contributor) / Department of Supply Chain Management (Contributor) / Morrison School of Agribusiness (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Throughout my experience in college, I learned many different techniques to communicate effectively. Most professors emphasized the importance of speaking clearly, and the ability to influence others. Dr. Kashiwagi piqued my interest when he explained his thoughts on how he wanted us to communicate to him. The criteria were simple, speak to him in a way that he could easily understand, without having to think. If thinking took place for him in the conversation, he determined that the person spoke too complexly and that his understanding of the student was low. After hearing this in class, I thought back to past conversations with my managers. I then wondered if I explained things clearer or simplified my wording, would things have gone better? I was also curious about simplicity in communication through writing, and how different presentations of information affected understanding. To further analyze these issues, I explored multiple research reports on verbal communication. Furthermore, I set up an experiment to test two common types of visual communication. The research concludes that Dr. Kashiwagi's theory was indeed correct, simplicity in conversation reduces miscommunication. The effectiveness of simplicity in written communication was partially proven by the survey results. The results indicated that the time required to fully understand a given topic dropped significantly if the information was depicted in a simplified format (list format). The more complex paragraph (textbook format) did have a higher level of understanding. However, the participants rated the textbook format job objectives as more complex, and stressful. After gathering the research, and running the experiment it can be concluded that by simplifying verbal communication, there are negligible differences in understanding of the topic, but the time of understanding decreases significantly.
ContributorsWilliams, Matthew Scott (Author) / Kashiwagi, Jacob (Thesis director) / Abraham, Seth (Committee member) / Department of Supply Chain Management (Contributor) / Barrett, The Honors College (Contributor)
Created2017-12
Description
Although there are some key qualities that all good leaders employ, variations in effective leadership approaches are evident across different cultures. This project sought to compare and analyze the differences and similarities in leadership principles between Chinese and American business cultures, with emphasis on the divergence caused by the influences of history, culture and politics.
ContributorsLe Tourneur, Maxine Archondakis (Author) / McKinnon, David (Thesis director) / LePine, Marcie (Committee member) / Department of Supply Chain Management (Contributor) / Department of Management and Entrepreneurship (Contributor) / Barrett, The Honors College (Contributor)
Created2017-12