The relationship between the characteristics of the urban land system and land surface temperature (LST) has received increasing attention in urban heat island and sustainability research, especially for desert cities. This research generally employs medium or coarser spatial resolution data and primarily focuses on the effects of a few classes of land-cover composition and pattern at the neighborhood or larger level using regression models. This study explores the effects of land system architecture—composition and configuration, both pattern and shape, of fine-grain land-cover classes—on LST of single family residential parcels in the Phoenix, Arizona (southwestern USA) metropolitan area. A 1 m resolution land-cover map is used to calculate land architecture metrics at the parcel level, and 6.8 m resolution MODIS/ASTER data are employed to retrieve LST. Linear mixed-effects models quantify the impacts of land configuration on LST at the parcel scale, controlling for the effects of land composition and neighborhood characteristics. Results indicate that parcel-level land-cover composition has the strongest association with daytime and nighttime LST, but the configuration of this cover, foremost compactness and concentration, also affects LST, with different associations between land architecture and LST at nighttime and daytime. Given information on land system architecture at the parcel level, additional information based on geographic and socioeconomic variables does not improve the generalization capability of the statistical models. The results point the way towards parcel-level land-cover design that helps to mitigate the urban heat island effect for warm desert cities, although tradeoffs with other sustainability indicators must be considered.

This study seeks to determine the role of land architecture—the composition and configuration of land cover—as well as cadastral/demographic/economic factors on land surface temperature (LST) and the surface urban heat island effect of Phoenix, Arizona. It employs 1 m National Agricultural Imagery Program data of land-cover with 120mLandsat-derived land surface temperature, decomposed to 30 m, a new measure of configuration, the normalized moment of inertia, and U.S. Census data to address the question for two randomly selected samples comprising 523 and 545 residential neighborhoods (census blocks) in the city. The results indicate that, contrary to most other studies, land configuration has a stronger influence on LST than land composition. In addition, both land configuration and architecture combined with cadastral, demographic, and economic variables, capture a significant amount of explained variance in LST. The results indicate that attention to land architecture in the development of or reshaping of neighborhoods may ameliorate the summer extremes in LST.

X-ray free-electron lasers provide novel opportunities to conduct single particle analysis on nanoscale particles. Coherent diffractive imaging experiments were performed at the Linac Coherent Light Source (LCLS), SLAC National Laboratory, exposing single inorganic core-shell nanoparticles to femtosecond hard-X-ray pulses. Each facetted nanoparticle consisted of a crystalline gold core and a differently shaped palladium shell. Scattered intensities were observed up to about 7 nm resolution. Analysis of the scattering patterns revealed the size distribution of the samples, which is consistent with that obtained from direct real-space imaging by electron microscopy. Scattering patterns resulting from single particles were selected and compiled into a dataset which can be valuable for algorithm developments in single particle scattering research.

Viral protein U (Vpu) is a type-III integral membrane protein encoded by Human Immunodeficiency Virus-1 (HIV- 1). It is expressed in infected host cells and plays several roles in viral progeny escape from infected cells, including down-regulation of CD4 receptors. But key structure/function questions remain regarding the mechanisms by which the Vpu protein contributes to HIV-1 pathogenesis. Here we describe expression of Vpu in bacteria, its purification and characterization. We report the successful expression of PelB-Vpu in Escherichia coli using the leader peptide pectate lyase B (PelB) from Erwinia carotovora. The protein was detergent extractable and could be isolated in a very pure form. We demonstrate that the PelB signal peptide successfully targets Vpu to the cell membranes and inserts it as a type I membrane protein. PelB-Vpu was biophysically characterized by circular dichroism and dynamic light scattering experiments and was shown to be an excellent candidate for elucidating structural models.