Matching Items (687)
Filtering by
- Creators: Dean, W.P. Carey School of Business
- Member of: Barrett, The Honors College Thesis/Creative Project Collection
Description
The coronavirus envelope (E) protein is a structural component of all coronavirus. Obtaining purified E protein in an efficient, clean, and reliable manner is needed for ongoing studies. Toward this goal the E protein of Mouse Hepatitis Virus (MHV) coronavirus was tagged with with either 6 or 10 histidine (his) residues which can be used for affinity chromatography purification. The his-tags were introduced by PCR into a cDNA of the MHV A59 virus strain at the carboxy end of the E gene. A reverse genetics approach was then used to assemble three full-length cDNAs of the viral genome, two modified with the hig-tags and a control wild-type (WT) without a tag . Full-length genomic RNAs were transcribed and electroporated into baby hamster kidney cells that express the MHV receptor (BHKR) and L2 rat lung cells. Virus was recovered after 72 h only from the 6X his-tagged genome and the WT control. Western blotting using antibodies against the E protein or the nucleocapsid (N) protein was performed after cells were infected with the recovered WT and 6X-tagged recombinant viruses. The E protein was not detected with the E antibody, but was detected with a histidine probe was used to detect the histidine residues. This indicates that the tagged protein is expressed and that the tag is present.
ContributorsHesser, Kathryn Sarah (Author) / Hogue, Brenda G. (Thesis director) / Hogue, Ian B. (Committee member) / Lim, Efram (Committee member) / Dean, W.P. Carey School of Business (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
As undergraduate students focus on the perceived adventure, growth opportunities, social connections, freedom and fun, they may not be aware of the dangers and risks associated with studying abroad. Despite university-created crisis prevention measures such as policies, documentation, and orientations warning of the risk of travel, students who study abroad face some form of a crisis every year. Universities warn travelers of the dangers of crime and the psychological issues associated with liminal experiences and culture shock, preparing students for the harsh reality that immersion into a foreign culture is an intense and sometimes taxing experience. Faculty and staff dedicate a tremendous amount of time and energy to ensure our students are braced for their travel experience, yet students still experience immense hardships. In a comprehensive analysis of this phenomenon, we seek to find and explore reasons and variables that account for this chasm. We suspect the reason for this chasm, despite good efforts, is the variance between the resources that are provided and needed both upon entry into host country, and re-entry into native country. In extensively reviewing existing scholarly literature, reviewing case studies, conducting examinations of multi-causal variables, and analyzing measurable data, we suggest that study abroad preparation resources must adapt in order to accommodate an ever-evolving undergraduate tourist experience. In Section I the research team provides an introduction and underscores the central question of the study. Section II includes an extensive literature review in order to establish a definition of culture shock, determine what universities currently do to mitigate culture shock and risk, and assess the efficacy of these strategies. The research team subsequently identifies a lacuna -- the gap or point of departure from existing literature and research that this study seeks to fill. Section III presents our hypothesis, while Section IV offers an outline of precise Methodology. Section V includes an in-depth Data Analysis using findings dependent upon surveys and interviews as discussed in Methodology. Section VI presents policy recommendations or a “fix” based upon findings presented in Data Analysis. Section VII presents a conclusion, offering a culmination of deductions and implications, proving the relevance of this study to Arizona State University.
ContributorsGrimes, Grace Cameron (Co-author) / Church, Elise (Co-author) / Iheduru, Okechukwu (Thesis director) / McWilliams, Claire (Committee member) / Dean, W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Hepatocellular carcinoma (HCC) is a type of liver cancer common in Sub-Saharan Africa and South East Asian countries. Each year more than 700,000 new cases and more than 600,000 deaths are recorded worldwide due to HCC. According to the American Cancer Society HCC is ranked the 5th most common cancer worldwide with a male:female susceptibility ratio ranging between 2:1 and 8:1. HCC risk factors include lifestyle behaviors, such as persistent alcohol abuse and smoking, prolonged exposure to aflatoxins, chronic viral hepatitis infections, inherited metabolic diseases and non-alcoholic fatty liver diseases. To understand the genetic effects underlying sex-bias in HCC, it is necessary to include the sex chromosomes in genomics analyses. X and Y chromosomes are often discluded in genomics studies because of the technical and analytical challenges: sequence homology. The purpose of this thesis is to analyze the effects of sex chromosome complement aware read mapping to germline variant calling. 10 male and 10 female tumor adjacent samples from The Cancer Genome Atlas Liver Hepatocellular Carcinoma (TCGA LIHC) cohort were processed using sex-aware and default reference and the concordance of the two approaches was examined. We detected a higher disconcordance of 0.69% on variants called on the X chromosome and a disconcordance of 0.51% on variants called on the Y chromosomes for the reference and alternative alleles respectively compared to autosomes. Variants called on the REF/ALT genotypes had a disconcordances of 1.00%, 1.05%, 1.35% and 12.34% for the autosomes, chromosome 7, the X, and the Y chromosome, respectively. At the end of the project we concluded that the generated datasets showed the effect of sex-aware read mapping on variant calling. Though the data did not show the sites that can be called as variants in one dataset but not in the other, rather the concordance looked at sites that were called as variants in both data sets.
ContributorsPhiri, Lovender Teresa (Co-author) / Phiri, Lovender (Co-author) / Wilson Sayres, Melissa (Thesis director) / Buetow, Kenneth (Committee member) / Natri, Heini (Committee member) / School of Life Sciences (Contributor) / Dean, W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
There are many parallels between human and equine sports medicine, including the roots of athletics, the development of specialized medicine, and injuries to the athletes. The most remarkable similarities are seen in tendinopathies. Because of the similarities between human and equine tendons, equine athletes serve as one of the best animal models to study tendon physiology and tendon injuries for application to human medicine. Because of this, many therapies have already successfully crossed from one realm of sports medicine to the other, the most notable of which are stem cell therapy, Interleukin-1 Receptor Antagonist (IRAP), and platelet rich plasma (PRP) therapies. There are also several therapies on the horizon that are very promising to potentially be successful in both human and equine athletes. However, some of these novel therapies are raising ethical questions. There are many regulations in place to protect against or address potential ethical conflicts in human medicine. The same trend is not seen in equine medicine. While there are rules enforced by many equestrian federations regarding ethical concerns and veterinary medicine, the information is not nearly as complete as it should be. Because they lack the autonomy that typical human patient possesses, equine athletes require significantly more advocacy from their veterinarians than human athletes do from their physicians. Additionally, there is a lack of large animal model studies secondary to cost and overall value of equine athletes to their owners, riders, and trainers. Ultimately it becomes an issue of veterinary ethics whether to pursue a novel or conventional treatment for an equine athlete. With biotechnology advancing as quickly as it is, new studies must be done and new regulations must be written in order to keep all fields of sports medicine operating safely and ethically for all athletes involved, regardless of species.
ContributorsWaslewski, Samantha Paige (Author) / Lynch, John (Thesis director) / Foley, James (Committee member) / School of Life Sciences (Contributor) / Dean, W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Usability in the Internet of Things (IOT) in the Smart Home is a new concept with limited current research, especially in user perception. User perception is an important aspect in the development of Smart Home Internet of Things systems, including smart home products and devices and in the design of the Smart Home experience. The aim of my thesis is to investigate user perceptions of Smart Home Internet of Things systems to understand how usable current Smart Home Internet of Things systems are from the perception of the user and how that impacts the Smart Home experience. By understanding the usability of Smart Home Internet of Things systems, we can better design Smart Home devices and products for a user-centered experience. My thesis informs the design of user-centered Smart Home Internet of Things systems and experiences.
This study includes a literature review and an empirical study. The empirical study is a usability testing survey that investigates user perceptions of Smart Home technology that make up the Internet of Things system in the Smart Home. Both closed-ended questions and open-ended questions are included in the survey for a comprehensive study. Main results showed that current smart home systems are somewhat usable. Results also showed that smart home users are satisfied with current smart home systems, believes that smart home technology adds value to their lifestyle, and will continue to use smart home technology. Main results showed that the strengths of current smart home systems design are efficiency, learnability, memorability, and enjoyability. Main results showed that there is a trend between age group and smart home user perceptions. These findings inform the design of user-centered smart home systems.
This study includes a literature review and an empirical study. The empirical study is a usability testing survey that investigates user perceptions of Smart Home technology that make up the Internet of Things system in the Smart Home. Both closed-ended questions and open-ended questions are included in the survey for a comprehensive study. Main results showed that current smart home systems are somewhat usable. Results also showed that smart home users are satisfied with current smart home systems, believes that smart home technology adds value to their lifestyle, and will continue to use smart home technology. Main results showed that the strengths of current smart home systems design are efficiency, learnability, memorability, and enjoyability. Main results showed that there is a trend between age group and smart home user perceptions. These findings inform the design of user-centered smart home systems.
ContributorsChuong, Samantha (Author) / Bowman, Diana (Thesis director) / Michael, Katina (Committee member) / Dean, W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
The classification of financially at-risk is an expansive term that fits the personal profile of most individuals when it comes to the conditioning of their attitude toward money management, particularly in the planning and investment of that money for the achievement of long-term goals. In the case of this thesis, we focus primarily on those who have made a career in professional athletics and entertainment. The behavioral finance tendencies of these two industry professions are widely regarded as insufficient and often damaging the to the longevity of achieved financial security. This ideology stems primarily from an environment where individuals enjoy rapid wealth accumulation in a highly competitive and constantly transitioning role within their respective crafts. The subjectively common behavioral shortcomings of these world-class athletes and performers and uncertain day-to-day security of the professions which these at-risk individuals possess make for highly unfavorable circumstances when striving to achieve a lifetime of income and a secure retirement. In examining individuals of these classes who have faced grave financial hardship, this thesis will serve as a basis for identifying measures to recondition problematic behavioral tendencies that ultimately cause disengagement from a prudent financial plan. Therefore, this thesis will also serve as a framework to determine what investment strategies will complement the behavioral modifications financial planners strive to instill in these individuals, so that professional athletes, celebrities, and financially at-risk professionals alike may achieve higher probability of creating financial freedom through the engaged execution of a goals-based financial plan.
ContributorsKeller, Charles Phillip (Author) / Licon, Wendell (Thesis director) / Budolfson, Arthur (Committee member) / Department of Supply Chain Management (Contributor) / Dean, W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Problem Statement
Company X does not currently have a proven hedging method that allows for forecasting flexibility and currency fluctuations, while still reducing the risk of de-designation in their international construction projects. To solve this issue, we will analyze historical currency fluctuations over the past 5 years, forecasting error rates, the impact of de-designation, and earnings per share impact.
Company X does not currently have a proven hedging method that allows for forecasting flexibility and currency fluctuations, while still reducing the risk of de-designation in their international construction projects. To solve this issue, we will analyze historical currency fluctuations over the past 5 years, forecasting error rates, the impact of de-designation, and earnings per share impact.
ContributorsBlackburn, Jamie Sue (Co-author) / Lopez, Ivan (Co-author) / Miller, Cole (Co-author) / Plocher, Jasmine (Co-author) / Sinacori, Anthony (Co-author) / Simonson, Mark (Thesis director) / Thomas, Jodi (Committee member) / Dean, W.P. Carey School of Business (Contributor) / Department of Finance (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
In this paper, I analyze the pharmaceutical supply chain to identify the main causes of drug surplus. The main example I use throughout the paper is the current opioid epidemic, which has resulted in thousands of fatalities, caused by overdoses. After researching the industry players and their relationships in the supply chain, I have identified four main causes of drug surplus: the consolidation of pharmaceutical corporations with third-party manufacturers, along with consolidation within the wholesaler industry; the inappropriate pricing of opioid-based prescriptions negotiated by pharmacy benefit managers (PBMs); the significant influence of pharmaceutical corporations on physicians, leading to potentially unethical practices; and lastly patients openly distributing leftover prescriptions to the market, and looking for prescriptions elsewhere. To alleviate the drug surplus issue, I provide three solutions: implement both blockchain and reverse logistics into the pharmaceutical supply chain, improving transparency, and allowing patients to return incomplete prescriptions; and research the consolidation of PBMs with providers (hospital systems, clinics, etc.) to increase buyer power and appropriately price opioid-based prescriptions.
ContributorsRutkowski, Sarah (Author) / Helm, Jonathan (Thesis director) / Wiedmer, Robert (Committee member) / Department of Information Systems (Contributor) / W.P. Carey School of Business (Contributor) / Department of Supply Chain Management (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
This thesis investigates the potential of life cycle analysis for more sustainable sourcing strategies in organizations. Using the example of the College of Lake County (CLC) in Illinois, I study how life-cycle analysis can help to improve the procurement of products and services in higher education. Currently, CLC's purchasing team does not understand how sourcing affects operational and environmental performance. In addition, CLC's purchasing team does not communicate effectively with other departments from a product utilization standpoint. The objective of this research is to analyze CLC's current product procurement process and to assess the feasibility of implementing life cycle analysis tools. Further, I evaluate different life cycle analysis tools and provide recommendations to CLC about the applicability of these tools so that they may be implemented into the university in the future. First, I find that both the procurement and IT department at CLC are not familiar with life-cycle analysis tools and hence, do not know about the life cycle of their processes and services. Second, I identify professional life cycle analysis tools relevant for CLC. Two software options, GaBi and SimaPro, are discussed. Finally, I suggest six steps for a successful implementation of life cycle analysis at CLC: (1) form an interdisciplinary team, (2) analyze demand and collect additional data, (3) conduct a product life cycle analysis using a software tool, (4) define which products to analyze further, (5) conduct life cycle costing analysis with the same software tool, and (6) utilize these results for decisions and delegation of responsibility.
ContributorsGotsch, Rachel Lynne (Author) / Wiedmer, Robert (Thesis director) / Kashiwagi, Jacob (Committee member) / Department of Supply Chain Management (Contributor) / Department of Finance (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
This thesis examines two electronic games, PlayerUnknown’s Battlegrounds (PUBG) and Fortnite, which rose to popularity as battle royale-style videogames, to question whether they are viable candidates for success as esports. In his 2018 Barrett Honors Thesis, Noah Hilliker developed a model for assessing electronic games as successful esports. His thesis, titled “Ambassador Communities, Hybrid Spectatorship, and Other Indicators of Success in eSports” applied his model of indicators of success to League of Legends, Overwatch and Hearthstone. In this thesis, I have applied his indicators to (PUBG) and Fortnite, which differ significantly from the games he previously analyzed, precisely as I argue here, because they are battle royale games. These indicators fall under three categories: the nature of fans, the overall design of the game and the relationship between game developers and fans. The data that was examined for this investigation included academic literature on esports, posts on online platforms, news articles, YouTube content and forums. The following indicators were analyzed in their application to the collected data for both games: self-organization, spectator-player hybridity, ambassadors, clear center of action, demonstration of skill, mechanical variance, game balance, character of fan/developer relationship and feedback to fans. Through this project, I apply a previous approach to analyzing esports success to two games, Fortnite and PUBG, in order to analyze how well the model fits battle royale-style games.
ContributorsSmith, Brenden (Author) / Ingram-Waters, Mary (Thesis director) / Schmidt, Peter (Committee member) / School of Life Sciences (Contributor) / Dean, W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05