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Glycosaminoglycan (GAG) binding by the cytokine pleiotrophin (PTN) was examined by expressing both thrombospondin 1 type-1 repeat domains of PTN separately, as PTN-N and PTN-C. PTN-N contains residues 31-89, and PTN-C contains residues 90-146. Nuclear magnetic resonance (NMR) experiments were conducted on both PTN-N and PTN-C to elucidate GAG binding

Glycosaminoglycan (GAG) binding by the cytokine pleiotrophin (PTN) was examined by expressing both thrombospondin 1 type-1 repeat domains of PTN separately, as PTN-N and PTN-C. PTN-N contains residues 31-89, and PTN-C contains residues 90-146. Nuclear magnetic resonance (NMR) experiments were conducted on both PTN-N and PTN-C to elucidate GAG binding regions. Titration with heparin dp6 showed a twofold increase in affinity when expressing PTN-N and PTN-C separately rather than as intact PTN. Paramagnetic relaxation rate enhancement experiments and surface paramagnetic relaxation enhancement (PRE) perturbation experiments were used to determine which residues were involved in GAG binding. One binding site was observed in PTN-N, around residue T82, and two binding sites were observed in PTN-C, one around residue K93 and the other around residue G142. These observed binding sites agree with the binding sites already proposed by the Wang lab group and other studies. Future work on the subject could be done on confirming that other varieties and length GAGs bind at the same sites, as well as examining the effect longer GAG fragments have on the affinity of intact PTN versus separate domains.
ContributorsKuch, Nathaniel Jacob (Author) / Wang, Xu (Thesis director) / Van Horn, Wade (Committee member) / School of Molecular Sciences (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2015-12
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Description
About 1% of the United States adult population currently suffers from schizophrenia. The symptoms of schizophrenia can be broken down into three main categories including: positive symptoms such as psychoses, negative symptoms such as anhedonia, and cognitive symptoms such as memory difficulties. The early growth response 3 (Egr3) is part

About 1% of the United States adult population currently suffers from schizophrenia. The symptoms of schizophrenia can be broken down into three main categories including: positive symptoms such as psychoses, negative symptoms such as anhedonia, and cognitive symptoms such as memory difficulties. The early growth response 3 (Egr3) is part of a family of genes known as the immediate early genes (IEGs), which are zinc-finger transcription factors. IEGs are not protein synthesis dependent, which means that they can be activated quickly, within 30-45 minutes, in response to certain environmental stimuli such as sleep deprivation. Egr3, an activity dependent gene, may be up-regulated by both genetic and environmental cues. Egr3 is thought to play an integral role in a biochemical pathway that may explain the onset of schizophrenia. However, the exact causes of schizophrenia remain unknown. Egr3 is not only activated in response to environmental factors, but has also been linked to many genes that are associated with schizophrenia in humans (Huentelman et al., 2015). Post-mortem brain tissue studies of patients with schizophrenia have decreased levels of EGR3 in their prefrontal cortex (PFC) and mice lacking Egr3 (Egr3 -/-) exhibit schizophrenia-like phenotypes such as locomotor hyperactivity. Egr 3 -/- mice also exhibit a diminished head twitch response to 2,5-Dimethoxy-4-iodoamphetamine (DOI), a 5-HT2A agonist (Yamada, et al., 2007; Gallitano-Mendel, et al., 2008). A link was established between schizophrenia patients and the serotonin 2A receptor (5-HT2AR) upon recognizing that 5-HT2AR agonists like lysergic acid diethylamide (LSD) create hallucinations similar to those in schizophrenic patients and 5-HT2AR antagonists such as the second-generation antipsychotic clozapine can reverse those hallucinations (Sommer, 2012). Paradoxically, however, post-mortem studies of schizophrenia patients have actually shown a decrease in PFC 5-HT2ARs as well as a 70% decrease found in the PFC of Egr3 -/- mice (Rasmussen, et al., 2010; Williams, et al., 2012). Therefore, we hypothesize that EGR3 directly regulates expression of 5-HT2ARs. To test this we will use virus-mediated overexpression of 5-HT2ARs in the PFCs of mice to see if we can rescue the schizophrenia-like phenotypes of the Egr3 -/- mice. After bilateral PFC stereotaxic injection of herpes simplex virus (HSV) with enhanced green fluorescent protein (EGFP) or HSV-Htr2a-EGFP in both wild type (WT) and Egr3 -/- mice, the mice were behaviorally tested using locomotor activity and DOI-induced head twitch response. We found that Egr3-/- mice, compared to WT mice, demonstrated locomotor hyperactivity and a decreased DOI-induced head twitch response, confirming prior findings, but no significant main effect of virus. A significant effect of the HSV-Htr2a-EGFP was seen when comparing DOI-induced head twitch response in WT mice to Egr3 -/- mice. WT mice showed a higher number of head twitches in comparison to the knockout mice. These findings suggest further research must be conducted in order to investigate whether a functional 5-HT2AR is being translated and correctly transported to the membrane. These findings may also point to an unknown factor mediating the regulation between Egr3 and 5-HT2ARs.
ContributorsHoebee, Shelby Marie (Author) / Van Horn, Wade (Thesis director) / Gallitano, Amelia (Committee member) / Department of Psychology (Contributor) / School of Molecular Sciences (Contributor) / School of Criminology and Criminal Justice (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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The aim of this study is to examine the relationship between Assisted Cycle Therapy, leisure time activity levels, fine motor control, and grip force in older adults with Down syndrome (DS), all of which affect activities of daily living (ADL) and therefore quality of life. This is relevant because this

The aim of this study is to examine the relationship between Assisted Cycle Therapy, leisure time activity levels, fine motor control, and grip force in older adults with Down syndrome (DS), all of which affect activities of daily living (ADL) and therefore quality of life. This is relevant because this particular group is at risk for developing early onset Alzheimer's disease (AD), which presents itself uniquely in this population. The parent or guardian of six participants with DS completed Godin's Leisure Time Exercise Questionnaire and the participants themselves completed Purdue Pegboard and grip force assessments before and after an 8-week exercise intervention. The results were inconsistent with past research, with no change being seen in fine motor control or grip force and a decrease being seen in leisure activity. These findings are indicative of the importance of the effect of fatigue on leisure activity as well as maintaining elevated heart rate throughout exercise interventions.
ContributorsGomez, Elizabeth Danielle (Author) / Ringenbach, Shannon (Thesis director) / Coon, David (Committee member) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor) / Department of Psychology (Contributor)
Created2015-05
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Description

Living a healthy and balanced life can be difficult for college students at Arizona State<br/>University due to the barrier of high cost of healthy food. To understand more about this problem<br/>we conducted research on the Tempe campus through surveys and virtual focus groups. We<br/>discovered that the cost of healthy food

Living a healthy and balanced life can be difficult for college students at Arizona State<br/>University due to the barrier of high cost of healthy food. To understand more about this problem<br/>we conducted research on the Tempe campus through surveys and virtual focus groups. We<br/>discovered that the cost of healthy food is one of the main barriers preventing students from<br/>eating healthy. We also learned that the students would be more willing to eat healthier if they<br/>could access healthy foods at a more reasonable price. Our solution to this problem was Eunoia,<br/>a service that allows students to receive discounts on healthy food and incentivize them to eat<br/>healthier in the future. Our company creates an innovative relationship between our customers,<br/>their private health insurance companies and local grocery stores throughout the Phoenix Metro<br/>area. Students at Arizona State University will be able to purchase healthy food items discounted<br/>by up to 30%. These discounts will be funded by their health insurance companies as well as the<br/>local grocery stores they purchase from. Our business model allows our customers to live<br/>healthier lives while also providing value to partnered health insurance companies and grocery<br/>stores. Once we established our business model, we spoke with students at Arizona State<br/>University and representatives from health insurance companies. Through these demographics,<br/>we received positive feedback and early traction with our idea. Our goal is to be able to<br/>implement our product in the Arizona State University community and then expand this product<br/>into a more general market to help all people live a pure and balanced life.

ContributorsEvans, Hayden Curtis (Co-author) / Wijesinghe, Megan (Co-author) / Zimprich, Preston (Co-author) / Byrne, Jared (Thesis director) / Hall, Rick (Committee member) / School of Molecular Sciences (Contributor) / Dean, W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description

In this formative research project, we seek to better understand the general barriers to refugee access to higher education. Using mixed methods research--which included surveys, interviews, and course data--we evaluate the benefits and challenges experienced by refugee students in Israel who are enrolled in Arizona State University's Education for Humanity

In this formative research project, we seek to better understand the general barriers to refugee access to higher education. Using mixed methods research--which included surveys, interviews, and course data--we evaluate the benefits and challenges experienced by refugee students in Israel who are enrolled in Arizona State University's Education for Humanity programs. In the end, this case study resulted in 24 recommend programmatic changes designed to eliminate the barriers that prevent refugee students from accessing and succeeding in higher education.

ContributorsJackman, Julia (Co-author) / Altaf, Amal (Co-author) / DeLargy, Pamela (Thesis director) / Mokwa, Michael (Committee member) / School of Civic & Economic Thought and Leadership (Contributor) / School of Molecular Sciences (Contributor) / School of Human Evolution & Social Change (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Description

Serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL) has enabled the determination of protein structures and protein reaction intermediates in millisecond to microsecond time resolutions. Mix-and-Inject crystallography (MISC) at XFELs enables fast mixing in the magnitude of milliseconds in order to achieve desired reaction time points. For these

Serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL) has enabled the determination of protein structures and protein reaction intermediates in millisecond to microsecond time resolutions. Mix-and-Inject crystallography (MISC) at XFELs enables fast mixing in the magnitude of milliseconds in order to achieve desired reaction time points. For these experiments, numerical simulations of a hydrodynamic flow mixer capable of fast mixing by diffusion has been developed using both COMSOL Multiphysics 5.6 and QuickerSims Computational Fluid Dynamics (CFD) Toolbox for MATLAB. These simulation programs were compared by calculations of mixing times and concentration flow profiles. Mixing times in the range of 1-10 ms were calculated in COMSOL under certain flow rate conditions whereas mixing times in the range of 6-15 ms were calculated with QuickerSims. From these mixing times, reaction intermediates can be varied from sub-millisecond to several hundred millisecond time points for a MISC experiment. Explanations for the discrepancies between the two models were attributed to variations in parameter definitions and meshing. Further analysis on the mixing characteristics were investigated by calculating an analytical solution to the convection-diffusion equation for fluid flow in a two-dimensional rectangular channel. The concentration profile along the width of the channel for the analytical solution was compared with the numerical solution obtained with COMSOL and QuickerSims. Upon comparison, it was determined that the diffusion coefficient may not be a significant factor for the disagreement between the two hydrodynamic flow models.

ContributorsGuzman, Manuel Alexander (Author) / Ros, Alexandra (Thesis director) / Williams, Peter (Committee member) / Hayes, Mark (Committee member) / School of Mathematical and Statistical Sciences (Contributor) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description

British colonialism was a well-established facet of Western history. The British have been especially notable in colonizing countries within Africa as well as India. In terms of India, the ramifications of their actions have had a significant impact on the social and political structure from the 19th century to modernity.

British colonialism was a well-established facet of Western history. The British have been especially notable in colonizing countries within Africa as well as India. In terms of India, the ramifications of their actions have had a significant impact on the social and political structure from the 19th century to modernity. Many scholars have alleged that brutalities set forth by the British and the discriminatory practices enforced onto the Indian population were entirely new. However, while the violence incurred cannot be ignored, the actual governance and structural changes were heavily influenced by the Hindu caste system already established within India. The Hindu caste system is a centuries-old practice that designates followers into five, family-determined, class denominations from highest to lowest: Brahmins (priests), Kshatriyas (rulers and warriors), Vaishyas (merchants, landowners, skilled workers), Shudras (farmworkers, servants, and unskilled workers), and Dalits (out-casts). The given thesis focuses on the perceptions and interactions of the British in 19th century India as well as the structural changes enforced by the British. Numerous nineteenth-century texts such as Up the Country by Emily Eden and India in 1848 by Arthur Mills, M.P. Murray were centered on the environment and day-to-day changes of India in the form of travel/informational readings. Both texts are utilized in this argument to highlight how the caste system was a contributing factor in British administration, contrary to the general perception of scholars. More importantly, Up the Country, offers a glimpse of the realities within northern India, where the British had the strongest control and is utilized to draw parallels between the caste system and British actions during the 1800s. The effects of discrimination and inequality continue to have a considerable effect on the Indian population to this day. However, the British have hastily been given total blame for such ramifications without considering the role of Indian societal principles in these disparities.

ContributorsNidamanuri, Sreecharita (Author) / Agruss, David (Thesis director) / Soares, Rebecca (Committee member) / School of Molecular Sciences (Contributor) / Dean, W.P. Carey School of Business (Contributor) / WPC Graduate Programs (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Description

Ants are widespread species of eusocial insects, and myrmecophily describes the species which are associated with ants. Many mites are myrmecophilous species and interact with hosts in many ways such as phoresis or parasitism. The relationship between ants and mites are interesting as parasitic species could be used to control

Ants are widespread species of eusocial insects, and myrmecophily describes the species which are associated with ants. Many mites are myrmecophilous species and interact with hosts in many ways such as phoresis or parasitism. The relationship between ants and mites are interesting as parasitic species could be used to control the spread of invasive ant species. For this project, I reviewed the existing literature on myrmecophilous mites around the world and compiled a database of ant-mite associations, which I then used to characterize factors such as host specificity, attachment sites, and biogeographical patterns. This work demonstrates that existing research on myrmecophilous mites has been both geographically and taxonomically biased and highlights the need for much more comprehensive surveys of mites living in association with ants.

ContributorsLin, Chan-An (Author) / Taylor, Jesse (Thesis director) / Rabeling, Christian (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Description

As much as SARS-CoV-2 has altered the way humans live since the beginning of 2020, this virus's deadly nature has required clinical testing to meet 2020's demands of higher throughput, higher accuracy and higher efficiency. Information technology has allowed institutions, like Arizona State University (ASU), to make strategic and operational

As much as SARS-CoV-2 has altered the way humans live since the beginning of 2020, this virus's deadly nature has required clinical testing to meet 2020's demands of higher throughput, higher accuracy and higher efficiency. Information technology has allowed institutions, like Arizona State University (ASU), to make strategic and operational changes to combat the SARS-CoV-2 pandemic. At ASU, information technology was one of the six facets identified in the ongoing review of the ASU Biodesign Clinical Testing Laboratory (ABCTL) among business, communications, management/training, law, and clinical analysis. The first chapter of this manuscript covers the background of clinical laboratory automation and details the automated laboratory workflow to perform ABCTL’s COVID-19 diagnostic testing. The second chapter discusses the usability and efficiency of key information technology systems of the ABCTL. The third chapter explains the role of quality control and data management within ABCTL’s use of information technology. The fourth chapter highlights the importance of data modeling and 10 best practices when responding to future public health emergencies.

ContributorsLeung, Michael (Co-author) / Kandan, Mani (Co-author) / Knox, Garrett (Co-author) / Woo, Sabrina (Co-author) / Compton, Carolyn (Thesis director) / Dudley, Sean (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Description

Apolipoprotein (ApoE) plays an important role in the transport of lipids in the brain for normal functioning. There are three different isoforms of ApoE which are coded for by three alleles (E2, E3, E4). Patients carrying at least one copy of ApoE E4 are known to be at higher

Apolipoprotein (ApoE) plays an important role in the transport of lipids in the brain for normal functioning. There are three different isoforms of ApoE which are coded for by three alleles (E2, E3, E4). Patients carrying at least one copy of ApoE E4 are known to be at higher risk for developing Alzheimer’s disease (AD) and earlier onset of symptoms. This is due to the buildup of amyloid plaques and neurofibrillary tangles of the brain from the accumulation of tau proteins, which are associated with the progression of Alzheimer’s disease. However, findings on ApoE E2 have shown that it may be a protective allele since it is linked to a decreased risk of formation of amyloid plaques and neurofibrillary tangles. To study this phenomenon within the context of a local population group, polymerase chain reaction and gel electrophoresis were conducted on extracted DNA samples. The principal goal in this research study was to genotype ApoE variants using single nucleotide polymorphism (SNP) specific primers, and polymerase chain reaction to analyze the frequency in the Tempe population to determine future healthcare needs.

ContributorsBernal, Miranda (Author) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05