People have known about mass biodiversity loss and the human actions that drive it for decades now, and yet we have largely failed levels to change our behavior to protect the environment. What’s failing to motivate people to change? Some conservation psychologists have partially blamed the negative way we communicate about environmental issues for paralyzing audiences into doing nothing because they feel helpless to change such a big problem. Instead, many psychologists have called for using positive emotions in communication to motivate an audience, but there’s still little research showing whether that’s a more effective approach or not. To study whether positive or negative emotions are really more motivational for inspiring change, I looked at how different emotions were used in the discourse about an emerging conservation technology called de-extinction as a case study. De-extinction claims to be both a tool for fighting biodiversity loss and for inspiring more positive and inspiring narratives in conservation. In this thesis, I examine those claims by exploring five emotions that the discourse around de-extinction elicits: fear, guilt, grief, awe and hope. I examined the motivating power of those emotions and what kind of actions de-extinction discourse motivates or fails to motivate through the way it uses those emotions. I found that de-extinction discourse erases negative emotions and boosts positive ones as many conservation psychologists recommend. However, de-extinction discourse accomplishes this in misleading ways: it minimizes the sense of importance of ongoing extinctions by framing extinction as a reversible phenomenon, and it overstates the ability of technology alone to combat the extinction crisis without requiring societal change. As a result, de-extinction discourse could risk making the public less motivated to take personal action to forward conservation goals. I conclude that positivity or negativity should not be the central concerns for motivating action, but rather efficacy and honesty.

In theory, Electric Vehicle (EV) ownership and renewable energy seem like a perfect solution to our climate crisis; however, unless done properly, the effects can be less than ideal. We need to find a way to maximize the impact of our efforts to reduce carbon emissions, which is exactly what the heart of my paper gets to. Carbon emissions are bad for the environment because they comprise a large majority of greenhouse gases. Greenhouse gases have recently become dramatically out of balance and have resulted in an increase in respiratory diseases from smog and air pollution, as well as extreme weather and an increase in wildfires. Getting these greenhouse gases back in balance and maintaining an ecological balance is the goal of sustainability. According to the Environmental Protection Agency (the EPA), transportation makes up 29% of greenhouse gas emissions in the US followed closely by electricity generation at 28%, which makes Electric Vehicles the perfect target for reducing greenhouse gas emissions<br/>Arizona has many unique constraints when it comes to its electric infrastructure and its electric generation energy mix, which means the impacts of EV ownership become extremely complicated.<br/> In my paper, I aim to address the question: What are the carbon impact effects of Electric Vehicles (EVs) in Arizona through the lens of 1) the time of day that charging occurs, 2) the infrastructure needed to support EV penetration and 3) the incentives given to the public to help provide the impetus for making greener choices? Using the best available research on how EVs are being adopted to reduce emissions, I will provide conclusive recommendations and a framework for how Arizona can best reduce carbon emissions through EVs.

The purpose of this research is to exploit the neglect of specific populations and diseases in Latin America through an epidemiological literature review. As a small part of a larger publication, the foci of this research was the infectious disease, helminthiasis. Using manually indexed abstracts from the National Library of Medicine database in PubMed, 4,594 papers were synthesized and then processed for further review. Of those papers, 29 provided information about helminths in indigenous populations. These papers were reviewed and used in prevalence data extraction and variable analysis. The main conclusion was to reveal the fact that from an entire health database less than 30 papers provided information about the persistence of helminths in indigenous communities of Latin America. Not only that but the few papers that could be analyzed had consistently high prevalence ratios.

In this thesis paper, the mental health consequences of the COVID-19 pandemic are discussed. Chapter 1 discusses what inspired me to write this thesis and follows with a discussion of social isolation during the COVID-19 pandemic. Chapter 2 takes a step back and discusses biological effects of social isolation in general. Chapter 3 discusses the psychological effects of social isolation. Finally, this thesis concludes with a discussion of what can be done to help those experiencing social isolation during the pandemic.

My research aims to determine the effectiveness of meditation and sleep applications (apps) on the reduction of anxiety and stress in college students, with a focus on sedative piano music. Results showed a significant reduction of stress and anxiety levels in college students when listening to sedative piano music versus non-sedative piano music. Music along with other therapy modalities in meditation and sleep apps show promise in reducing students’ anxiety and stress and promoting their successes.

The microbiome and the immune system are known to work in conjunction to modulate the clearance of pathogens and tolerance of beneficial microbes. A growing area of research seeks to study the potential extent of the involvement of the microbiome in modulating and supporting the immune system during acute allograft rejection. It has been hypothesized that the localized microbiota in each organ produce metabolites that instigate inflammatory immune responses, but whether microbiota interactions precipitate acute allograft rejection is unknown. Therefore, this study focuses on microbiome shifts in the gut and kidney after inducing acute renal transplant rejection in order to implicate gut dysbiosis as a precursor or supporter of allograft rejection. This study also subsequently explores the use of an immune-modulating protein in order to determine differences in the outcome of transplant rejection and potential differences in intestinal microbial load. This experiment sought to induce rejection in BALB/c mice through the use of C57BL/6 mouse renal slivers. Microbiome abundance was analyzed in all experimental groups. Understanding the role of the microbiome in transplant rejection has vast clinical implications and has the potential to enhance pre- and post-operative treatment, and immune management and quality of life following organ transplant.

Generating an astounding $110.7 billion annually in domestic revenue alone [1], the world of accounting is one deceptively lacking automation of its most business-critical processes. While accounting tools do exist for the common person, especially when it is time to pay their taxes, such innovations scarcely exist for many larger industrial tasks. Exceedingly common business events, such as Business Combinations, are surprisingly manual tasks despite their $1.1 trillion valuation in 2020 [2]. This work presents the twin accounting solutions TurboGAAP and TurboIFRS: an unprecedented leap into these murky waters in an attempt to automate and streamline these gigantic accounting tasks once entrusted only to teams of experienced accountants.
A first-to-market approach to a trillion-dollar problem, TurboGAAP and TurboIFRS are the answers for years of demands from the accounting sector that established corporations have never solved.

"Generating an astounding $110.7 billion annually in domestic revenue alone [1], the world of accounting is one deceptively lacking automation of its most business-critical processes. While accounting tools do exist for the common person, especially when it is time to pay their taxes, such innovations scarcely exist for many larger industrial tasks. Exceedingly common business events, such as Business Combinations, are surprisingly manual tasks despite their $1.1 trillion valuation in 2020 [2]. This work presents the twin accounting solutions TurboGAAP and TurboIFRS: an unprecedented leap into these murky waters in an attempt to automate and streamline these gigantic accounting tasks once entrusted only to teams of experienced accountants.
A first-to-market approach to a trillion-dollar problem, TurboGAAP and TurboIFRS are the answers for years of demands from the accounting sector that established corporations have never solved."

Glioblastoma (GB) is one of the deadliest cancers and the most common form of adult primary brain tumors. SGEF (ARHGEF26) has been previously shown to be overexpressed in GB tumors, play a role in cell invasion/migration, and increase temozolomide (TMZ) resistance.[3] It was hypothesized parental LN229 cell lines with SGEF knockdown (LN229-SGEFi) will show decreased metabolism in the MTS assay and decreased colony formation in a colony formation assay compared to parental LN229 cells after challenging the two cell lines with TMZ. For WB and co-immunoprecipitation (co-IP), parental LN229 cells with endogenous SGEF and BRCA were expected to interact and stain in the BRCA1:IP WB. LN229-SGEFi cells were expected to show very little SGEF precipitated due to shRNA targeted knockdown of SGEF. In conditions with mutations in the BRCA1 binding site (LN229-SGEFi + AdBRCAm/AdDM), SGEF expression was expected to decrease compared to parental LN229 or LN229-SGEFi cells reconstituted with WT SGEF (LN229-SGEFi + AdWT). LN229 infected with AdSGEF with a mutated nuclear localization signal (LN229-SGEFi + AdNLS12m) were expected to show BRCA and SGEF interaction since whole cell lysates were used for the co-IP. MTS data showed no significant differences in metabolism between the two cell lines at all three time points (3, 5, and 7 days). Western blot analysis was successful at imaging both SGEF and BRCA1 protein bands from whole cell lysate. The CFA showed no significant difference between cell lines after being challenged with 500uM TMZ. The co-IP immunoblot showed staining for BRCA1 and SGEF for all lysate samples, including unexpected lysates such as LN229-SGEFi, LN229-SGEFi + AdBRCAm, and LN229-SGEFi + AdDM. These results suggested either an indirect protein interaction between BRCA1 and SGEF, an additional BRCA binding site not included in the consensus, or possible detection of the translocated SGEF in knockdown cells lines since shRNA cannot enter the nucleus. Further optimization of CO-IP protocol, MTS assay, and CFA will be needed to characterize the SGEF/BRCA1 interaction and its role in cell survival.

As part of Arizona State University’s net-zero carbon initiative, 1000 mesquite trees were planted on a vacant plot of land at West Campus to sequester carbon from the atmosphere. Urban forestry is typically a method of carbon capture in temperate areas, but it is hypothesized that the same principle can be employed in arid regions as well. To test this hypothesis a carbon model was constructed using the pools and fluxes measured at the Carbon sink and learning forest at West Campus. As an ideal, another carbon model was constructed for the mature mesquite forest at the Hassayampa River Preserve to project how the carbon cycle at West Campus could change over time as the forest matures. The results indicate that the West Campus plot currently functions as a carbon source while the site at the Hassayampa river preserve currently functions as a carbon sink. Soil composition at both sites differ with inorganic carbon contributing to the largest percentage at West Campus, and organic carbon at Hassayampa. Predictive modeling using biomass accumulation estimates and photosynthesis rates for the Carbon Sink Forest at West Campus both predict approximately 290 metric tons of carbon sequestration after 30 years. Modeling net ecosystem exchange predicts that the West Campus plot will begin to act as a carbon sink after 33 years.