Matching Items (27)

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Surgical procedures and postsurgical tissue processing significantly affect expression of genes and EGFR-pathway proteins in colorectal cancer tissue

Description

An understanding of tissue data variability in relation to processing techniques during and postsurgery would be desirable when testing surgical specimens for clinical diagnostics, drug development, or identification of predictive

An understanding of tissue data variability in relation to processing techniques during and postsurgery would be desirable when testing surgical specimens for clinical diagnostics, drug development, or identification of predictive biomarkers.
Specimens of normal and colorectal cancer (CRC) tissues removed during colon and liver resection surgery were obtained at the beginning of surgery and postsurgically, tissue was fixed at 10, 20, and 45 minutes. Specimens were analyzed from 50 patients with primary CRC and 43 with intrahepatic metastasis of CRC using a whole genome gene expression array. Additionally, we focused on the epidermal growth factor receptor pathway and quantified proteins and their phosphorylation status in relation to tissue processing timepoints.
Gene and protein expression data obtained from colorectal and liver specimens were influenced by tissue handling during surgery and by postsurgical processing time. To obtain reliable expression data, tissue processing for research and diagnostic purposes needs to be highly standardized.

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Date Created
  • 2014-11-03

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ING4 Tumor Suppressor and Chemotherapy Response in Triple-negative Breast Cancer

Description

Triple-negative breast cancer (TNBC) is defined by the lack of three receptors (estrogen, progesterone, and HER2 receptors) and accounts for 12-17% of breast cancers. TNBC is an aggressive form

Triple-negative breast cancer (TNBC) is defined by the lack of three receptors (estrogen, progesterone, and HER2 receptors) and accounts for 12-17% of breast cancers. TNBC is an aggressive form of the disease associated with high rates of recurrence and mortality within five years. Inhibitor of Growth 4 (ING4) is a gene deleted in 16.5% and downregulated in 34% of breast tumors. The correlation between ING4 deficiencies and advanced tumors and poor patient survival implicates its tumor suppressive function in breast cancer. Low ING4 expression has been correlated with NFκB activation in metastatic breast tumors. Moreover, ING4 has been shown to inhibit NFkB-mediated gene transcription in various cancers, suggesting that ING4 may suppress cancer by inhibiting NFkB activation. However, the contribution of ING4 deficiencies and NFkB activation to aggressive TNBC progression is currently not well understood. We investigated the role of ING4 in the MDAmb231 TNBC cell line by genetically engineering the cells to overexpress or delete ING4. Cell growth and sensitivity to the chemotherapeutic agent doxorubicin were evaluated between the ING4-modified cell lines with or without TNFα to activate NFκB. The results showed that cell growths were comparable between the vector controls and ING4 overexpressing or deleted cell lines. In addition, TNFα treatment did not alter the growths of all cell lines, indicating that ING4 with or without NFkB activation did not play a role in determining the growth rates of TNBC. However, ING4 overexpressing cells were 20-30% more sensitive to 10 μM doxorubicin treatment, whereas ING4-deleted cells were 20-50% more resistant, suggesting that ING4 may determine chemotherapy response in TNBC. These findings suggest that tumors with low levels of ING4 may be more resistant to chemotherapy, thus requiring higher dosage and/or additional chemotherapy in patient treatment. Unexpectedly, TNFα sensitized all cell lines to doxorubicin regardless of ING4 expression levels, suggesting a TNFα function outside of NFκB activation in increasing doxorubicin sensitivity. It implicates that TNFα treatment may increase chemotherapy response in TNBC patients.

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Date Created
  • 2017-12

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The Relationship Between Healthcare Provider’s Physical Activity and Predictive Patient Care Treatment Plans

Description

This research evaluates the need for health providers to prioritize their personal health as a means to improve their patient care. Due to the traditional healthcare system maintaining a patient-centric

This research evaluates the need for health providers to prioritize their personal health as a means to improve their patient care. Due to the traditional healthcare system maintaining a patient-centric focus, physicians became victims to the very diseases they were treating their patents for. The sacrifice of one's own health caused physicians to be more susceptible to both institutional and perceptual barriers that limited their engagement in preventative care counseling. Their own personal lifestyle habits, such as physical activity, played an influential role when prescribing treatment plans, and thus, could serve as a compromising factor in substandard care of a patient. The research suggested that providers who sustained healthier lifestyles by practicing what they preach are more efficient at delivering quality care to their patients in comparison to providers living an unhealthy lifestyle. With a provider's responsibility and obligation to continuously provide optimal care, there is a need to promote the health of a provider to establish both reliable and standardize patient care within the healthcare system. In addition to the research, three personal testimonials are included to help demonstrate the potential effects of a physician’s personal health in their medical practice.

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Created

Date Created
  • 2020-05

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Hepatitis C Prevention Measures in Pakistan

Description

Hepatitis C virus (HCV) is endemic in Pakistan, with 5% of the population suffering from the disease. A unique aspect about HCV in Pakistan is the major role that healthcare

Hepatitis C virus (HCV) is endemic in Pakistan, with 5% of the population suffering from the disease. A unique aspect about HCV in Pakistan is the major role that healthcare workers play in its transmission, by reusing needles and giving therapeutic injections when they are not needed. This issue is furthered by patients’ misconceptions that invasive treatments, like injections, are more effective than oral medicines. The purpose of this project was to create a short video that addressed this inaccurate and dangerous perception, by educating Pakistanis about HCV and how to prevent infection and reinfection. In addition to disease transmission, accessibility to treatment options in Pakistan were also discussed. The video featured Pakistani physicians and some young adults. There were several limitations that delimited the project, including time, budget, the sudden death of a project participant, and the current COVID-19 epidemic as well as cultural, language, and physical barriers that come from filming a video about Pakistan as Americans. In the future, this video can serve as a framework for future efforts.

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Created

Date Created
  • 2020-05

Human Biospecimen Preanalytics

Description

The purpose of this thesis creative project was to create an educational video to present research findings on the increasingly important issue of human biospecimen preanalytic variables. When a human

The purpose of this thesis creative project was to create an educational video to present research findings on the increasingly important issue of human biospecimen preanalytic variables. When a human biospecimen, such as blood, urine, or tissue, is removed from the body, it is subjected to a plethora of variables that are not recorded or regulated in a vast majority of cases. Frequently, these samples arrive at the research or pathology lab with an unknown history, then undergo analysis for translational research purposes, or to guide clinical management decisions. Thus, compromised specimen quality caused by preanalytic variables has substantial, and potentially devastating, downstream effects. To identify the preanalytic variables with the greatest impact on blood and tissue specimen quality, 45 articles were gathered using PubMed and Google Scholar databases and cited. Based on the articles, the top five variables with the most detrimental effects were identified for both blood and tissue samples. Multiple sets of parameters ensuring specimen fitness were compared for each of the five variables for each specimen type. Then, specific parameters guaranteeing the fitness of the greatest number of analytes were verified. To present the research findings in greater detail, a paper was written that focused on identifying the top variables and key parameters to ensure analyte fitness. To present the overall issue in an easy-to-digest format, a storyboard and script were created as a guideline for a final video project. Ultimately, two alternate versions of the video were created to pertain to the audience of choice (one version for patients, one version for professionals). It is the hope that these videos will be used as educational tools to continue efforts to standardize and enforce human biospecimen preanalytic variable parameters. This is a necessary step to improve the accuracy of our biomedical research data and the healthcare of patients worldwide.

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Created

Date Created
  • 2018-12

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Monitoring the Molecular Quality of Biopsy Tissue Samples Using Volatile Organic Compounds

Description

A major challenge with tissue samples used for biopsies is the inability to monitor their molecular quality before diagnostic testing. When tissue is resected from a patient, the cells are

A major challenge with tissue samples used for biopsies is the inability to monitor their molecular quality before diagnostic testing. When tissue is resected from a patient, the cells are removed from their blood supply and normal temperature-controlled environment, which causes significant biological stress. As a result, the molecular composition and integrity undergo significant change. Currently, there is no method to track the effects of these artefactual stresses on the sample tissue to determine any deviations from the actual patient physiology. Without a way to track these changes, pathologists have to blindly trust that the tissue samples they are given are of high quality and fit for molecular analysis; physicians use the analysis to make diagnoses and treatment plans based on the assumption that the samples are valid. A possible way to track the quality of the tissue is by measuring volatile organic compounds (VOCs) released from the samples. VOCs are carbon-based chemicals with high vapor pressure at room temperature. There are over 1,800 known VOCs within humans and a number of these exist in every tissue sample. They are individualized and often indicative of a person’s metabolic condition. For this reason, VOCs are often used for diagnostic purposes. Their usefulness in diagnostics, reflectiveness of a person’s metabolic state, and accessibility lends them to being beneficial for tracking degradation. We hypothesize that there is a relationship between the change in concentration of the volatile organic compounds of a sample, and the molecular quality of a sample. This relationship is what would indicate the accuracy of the tissue quality used for a biopsy in relation to the tissue within the body.

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Created

Date Created
  • 2020-05

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The Making of a COVID-19 Lab: A Business Exploration

Description

For our project, we explored the growth of the ASU BioDesign Clinical Testing Laboratory (ABCTL) from a standard university research lab to a COVID-19 testing facility through a business lens.

For our project, we explored the growth of the ASU BioDesign Clinical Testing Laboratory (ABCTL) from a standard university research lab to a COVID-19 testing facility through a business lens. The lab has pioneered the saliva-test in the Western United States. This thesis analyzes the laboratory from various business concepts and aspects. The business agility of the lab and it’s quickness to innovation has allowed the lab to enjoy great success. Looking into the future, the laboratory has a promising future and will need to answer many questions to remain the premier COVID-19 testing institution in Arizona.

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Created

Date Created
  • 2021-05

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The Making of a COVID-19 Lab: A Business Exploration

Description

For our project, we explored the growth of the ASU BioDesign Clinical Testing Laboratory (ABCTL) from a standard university research lab to a COVID-19 testing facility through a business lens.

For our project, we explored the growth of the ASU BioDesign Clinical Testing Laboratory (ABCTL) from a standard university research lab to a COVID-19 testing facility through a business lens. The lab has pioneered the saliva-test in the Western United States. This thesis analyzes the laboratory from various business concepts and aspects. The business agility of the lab and it’s quickness to innovation has allowed the lab to enjoy great success. Looking into the future, the laboratory has a promising future and will need to answer many questions to remain the premier COVID-19 testing institution in Arizona.

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Agent

Created

Date Created
  • 2021-05

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The Making of a COVID-19 Lab: A Business Exploration

Description

For our project, we explored the growth of the ASU BioDesign Clinical Testing Laboratory (ABCTL) from a standard university research lab to a COVID-19 testing facility through a business lens.

For our project, we explored the growth of the ASU BioDesign Clinical Testing Laboratory (ABCTL) from a standard university research lab to a COVID-19 testing facility through a business lens. The lab has pioneered the saliva-test in the Western United States. This thesis analyzes the laboratory from various business concepts and aspects. The business agility of the lab and it’s quickness to innovation has allowed the lab to enjoy great success. Looking into the future, the laboratory has a promising future and will need to answer many questions to remain the premier COVID-19 testing institution in Arizona.

Contributors

Agent

Created

Date Created
  • 2021-05

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The Making of a COVID-19 Lab: A Business Exploration

Description

For our project, we explored the growth of the ASU BioDesign Clinical Testing Laboratory (ABCTL) from a standard university research lab to a COVID-19 testing facility through a business lens.

For our project, we explored the growth of the ASU BioDesign Clinical Testing Laboratory (ABCTL) from a standard university research lab to a COVID-19 testing facility through a business lens. The lab has pioneered the saliva-test in the Western United States. This thesis analyzes the laboratory from various business concepts and aspects. The business agility of the lab and it’s quickness to innovation has allowed the lab to enjoy great success. Looking into the future, the laboratory has a promising future and will need to answer many questions to remain the premier COVID-19 testing institution in Arizona.

Contributors

Agent

Created

Date Created
  • 2021-05