Matching Items (347)
Description
Premise of the study: Land-plant plastid genomes have only rarely undergone significant changes in gene content and order. Thus, discovery of additional examples adds power to tests for causes of such genome-scale structural changes.
Methods: Using next-generation sequence data, we assembled the plastid genome of saguaro cactus and probed the nuclear genome for transferred plastid genes and functionally related nuclear genes. We combined these results with available data across Cactaceae and seed plants more broadly to infer the history of gene loss and to assess the strength of phylogenetic association between gene loss and loss of the inverted repeat (IR).
Key results: The saguaro plastid genome is the smallest known for an obligately photosynthetic angiosperm (∼113 kb), having lost the IR and plastid ndh genes. This loss supports a statistically strong association across seed plants between the loss of ndh genes and the loss of the IR. Many nonplastid copies of plastid ndh genes were found in the nuclear genome, but none had intact reading frames; nor did three related nuclear-encoded subunits. However, nuclear pgr5, which functions in a partially redundant pathway, was intact.
Conclusions: The existence of an alternative pathway redundant with the function of the plastid NADH dehydrogenase-like complex (NDH) complex may permit loss of the plastid ndh gene suite in photoautotrophs like saguaro. Loss of these genes may be a recurring mechanism for overall plastid genome size reduction, especially in combination with loss of the IR.
Methods: Using next-generation sequence data, we assembled the plastid genome of saguaro cactus and probed the nuclear genome for transferred plastid genes and functionally related nuclear genes. We combined these results with available data across Cactaceae and seed plants more broadly to infer the history of gene loss and to assess the strength of phylogenetic association between gene loss and loss of the inverted repeat (IR).
Key results: The saguaro plastid genome is the smallest known for an obligately photosynthetic angiosperm (∼113 kb), having lost the IR and plastid ndh genes. This loss supports a statistically strong association across seed plants between the loss of ndh genes and the loss of the IR. Many nonplastid copies of plastid ndh genes were found in the nuclear genome, but none had intact reading frames; nor did three related nuclear-encoded subunits. However, nuclear pgr5, which functions in a partially redundant pathway, was intact.
Conclusions: The existence of an alternative pathway redundant with the function of the plastid NADH dehydrogenase-like complex (NDH) complex may permit loss of the plastid ndh gene suite in photoautotrophs like saguaro. Loss of these genes may be a recurring mechanism for overall plastid genome size reduction, especially in combination with loss of the IR.
ContributorsSanderson, Michael J. (Author) / Copetti, Dario (Author) / Burquez, Alberto (Author) / Bustamante, Enriquena (Author) / Charboneau, Joseph L. M. (Author) / Eguiarte, Luis E. (Author) / Kumar, Sudhir (Author) / Lee, Hyun Oh (Author) / Lee, Junki (Author) / McMahon, Michelle (Author) / Steele, Kelly (Author) / Wing, Rod (Author) / Yang, Tae-Jin (Author) / Zwickl, Derrick (Author) / Wojciechowski, Martin (Author) / College of Integrative Sciences and Arts (Contributor) / College of Liberal Arts and Sciences (Contributor) / School of Life Sciences (Contributor)
Created2015-07-01
Description
This survey was used to identify the concerns of the public. There are many population techniques that the public feels are harmful to the behavior and nature of the horses. This research defined the different techniques and history of the situation to then analyze the public's opinion of the topic. The survey was trying to determine if any of the techniques could be accepted by the public to control the population of the horses that won’t put the forest, other species, and the herds themselves in danger of possible overpopulation effects.
The data collected from this research showed conflict for the subject. Some of the data was skewed due to conflicting viewpoints on the topic. Analyzing the other aspects of the data we saw noticed that 73% of the servers felt the horses were wild. A majority agreed that the horses should stay there and not be relocated. Some individuals who took the survey were interested in adopting out the horses to help manage horses, there were some concerns with background checks for these adopters since slaughter houses and poor living conditions is a concern.
The data collected from this research showed conflict for the subject. Some of the data was skewed due to conflicting viewpoints on the topic. Analyzing the other aspects of the data we saw noticed that 73% of the servers felt the horses were wild. A majority agreed that the horses should stay there and not be relocated. Some individuals who took the survey were interested in adopting out the horses to help manage horses, there were some concerns with background checks for these adopters since slaughter houses and poor living conditions is a concern.
ContributorsPadayachee, Brittany (Co-author) / Hoover, Sierra (Co-author) / Roen, Duane (Thesis director) / Murphree, Julie (Committee member) / College of Integrative Sciences and Arts (Contributor) / Barrett, The Honors College (Contributor)
Created2020-12
Description
This survey was used to identify the concerns of the public. There are many population techniques that the public feels are harmful to the behavior and nature of the horses. This research defined the different techniques and history of the situation to then analyze the public's opinion of the topic. The survey was trying to determine if any of the techniques could be accepted by the public to control the population of the horses that won’t put the forest, other species, and the herds themselves in danger of possible overpopulation effects.
The data collected from this research showed conflict for the subject. Some of the data was skewed due to conflicting viewpoints on the topic. Analyzing the other aspects of the data we saw noticed that 73% of the servers felt the horses were wild. A majority agreed that the horses should stay there and not be relocated. Some individuals who took the survey were interested in adopting out the horses to help manage horses, there were some concerns with background checks for these adopters since slaughter houses and poor living conditions is a concern.
The data collected from this research showed conflict for the subject. Some of the data was skewed due to conflicting viewpoints on the topic. Analyzing the other aspects of the data we saw noticed that 73% of the servers felt the horses were wild. A majority agreed that the horses should stay there and not be relocated. Some individuals who took the survey were interested in adopting out the horses to help manage horses, there were some concerns with background checks for these adopters since slaughter houses and poor living conditions is a concern.
ContributorsHoover, Sierra Nicole (Co-author) / Padayachee, Brittany (Co-author) / Roen, Duane (Thesis director) / Murphree, Julie (Committee member) / College of Integrative Sciences and Arts (Contributor) / Barrett, The Honors College (Contributor)
Created2020-12
Description
The purpose of this project was to create a workshop for adults without dance experience to immerse them in dance as a form of therapy. The goals of this workshop are to foster physical awareness and physical creativity. There are four main areas the participants would get experience in from this workshop including self-care/awareness, expression, gesture and choreography. These four areas are the ways that the two main goals manifest themselves. For this project I reviewed research by many different professionals from the therapy, psychology, self-care, dance, and dance therapy fields. Their studies as well as my own experience aided in putting together my themes for the workshop and class activities. I include my methodology, lesson plans, a workbook, and my reflection on the process.
ContributorsWilson, Deepika (Author) / Roses-Thema, Cynthia (Thesis director) / Giorgis, Cyndi (Committee member) / College of Integrative Sciences and Arts (Contributor) / Barrett, The Honors College (Contributor)
Created2020-12
Description
Particulate matter (PM) air pollution is a known factor to exacerbate cardiopulmonary diseases. We previously demonstrated that PM mediated endothelial injury and barrier disruption via modulation of the endothelial cytoskeleton and cell-cell junctions, while the effects of PM exposure on cell-cell communication and gap junction activity are still unknown. This study is focused on the characterization of PM-mediated endothelial dysfunction via Connexin 43 (Cx43), the most abundant Gap junction protein expressed in lung endothelial cells (ECs). PM exposure induces a time-dependent elevation of Cx43 in human lung ECs, at both mRNA and protein levels. N-acetyl-cysteine (NAC), an ROS scavenger, significantly suppresses PM-induced Cx43 expression. Membrane-associated and ER/ Golgi apparatus Cx43 protein are elevated upon PM challenge. In addition, PM also activates the gap junction activity, indicated by the transportation of green fluorescence dye between two adjacent ECs. Moreover, GAP27, a selective Cx43 channel inhibitor, attenuates PM-reduced human lung EC barrier disruption, measured by trans-endothelial electrical resistance (TER) with an electric cell-substrate impedance sensing system. Moreover, knock-down the expression of Cx43 by its selective siRNA alleviates PM-induced MLC phosphorylation. These results highly suggest that Cx43 plays a key role in PM-mediated endothelial barrier disruption and signal transduction. Cx43 may deputy as a therapeutic target in PM-mediated cardiopulmonary disorders.
ContributorsKheshtchin-Kamel, Nabia (Author) / Welcome, Natalie (Thesis director) / Wang, Ting (Committee member) / College of Integrative Sciences and Arts (Contributor) / Barrett, The Honors College (Contributor)
Created2020-12
Description
“Sam’s Emotional Day: A Child’s Guide to Recognizing and Handling Emotions” is a creative project that fulfills the thesis requirement for Barrett, The Honors College at Arizona State University. It is an illustrated children’s book meant to help children ages 4-7 identify, understand, and manage the six basic emotions. The illustrations of the main character throughout the book display clear facial expressions associated with each emotion, which were designed for children to easily identify them. The text in the story portion will prompt children to understand the feelings behind each emotion in a simple matter, and the end of the book provides techniques for them to manage emotions. Research on the importance of teaching children emotions, how to teach them, and similar children’s literature are discussed. Facial expressions and feelings while experiencing an emotion was also researched, which is presented in the creative process. An analysis of the text and illustrations is provided as well.
ContributorsMartinson, Hannah Marie (Author) / Jimenez-Arista, Laura (Thesis director) / Pereira, Jennifer (Committee member) / College of Integrative Sciences and Arts (Contributor) / Barrett, The Honors College (Contributor)
Created2020-12
Description
The United Nations outlined seventeen goals in their 2015 resolution aimed at securing a more sustainable future for the entire world. These goals cover all parts of life and are meant to standardize quality of life at a high level for all countries. Specific to the Maasai people of Kenya and Tanzania, there are five main goals that would tackle some of the biggest issues facing the Maasai and greatly improve their lives. These goals include the promotion of good health and well-being, ensuring a quality education for school-age children, supporting efforts for gender equality in all communities, and securing clean and safe water and sanitation services for all people around the world. There are many Non-Governmental Organizations (NGOs) in the Maasai community that are striving to support them and realize these Sustainable Development Goals. Organizations like the Maasai Girls Education Fund and Aid Tanzania have established educational programs to support children, mainly girls, in their efforts to get an education. Others like End Water Poverty and the Maasai Association have programs to ensure safe, accessible drinking water is made available to Maasai communities across Kenya and Tanzania. Lastly, NGOs like My Chosen Vessels have programs that support the economic sustainability and livestock management of the Maasai while also ensuring that Maasai families receive clean water and access to an education. The work and practices of NGOs like these can serve as examples of how to efficiently and effectively address and tackle issues in developing countries, specifically regarding Maasai women and girls, to move closer to achieving these UN Sustainable Development Goals and lead a more sustainable future. Many studies have been done researching the political and economic struggles for Maasai communities and how it impacts them. Vandana Shiva coined the term, “Water Democracy” in her book Water Wars to express the multiple reasons why water is a necessity and right. Many Maasai groups do not have easy access to basic essentials for their everyday lives and NGOs have been noticing this more and more. Dorothy Hodgson writes about the history of Maasai NGOs, and mentions the multiple conflicts that they have had with each other, causing many Maasai groups to have different lifestyles. The connections between the history of the Maasai tribe, their current economy and their current relationship with the government and NGOs all impact women in their communities in different ways.
ContributorsWacloff, Jianna K (Author) / Martin, Thomas (Thesis director) / Oberle, Eric (Committee member) / College of Integrative Sciences and Arts (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
This policy proposal paper is designed to address concerns about the protection of data
concerning citizens of the United States. The first step is to explore the need for federal
legislation because of the problems of cyberattacks, data loss and leakage, and big data. The
proposal then analyses how other countries had already addressed these concerns for their
citizens through legislation by looking at their regulation and the results of implementation. The
paper adjacently discusses the importance of American values of privacy as a fundamental right,
the free market, and protection from the private sector within a cybersecurity paradigm. From
this combined research, the paper yields a proposal of how the U.S. government should address
the situation through federal policy. The policy outlines cybersecurity measures to protect
information from cyberattacks and data loss and leakage, rights of American citizens that
organizations need to uphold, and the creation of a commission that provides resources and
education to domestic and foreign organizations.
concerning citizens of the United States. The first step is to explore the need for federal
legislation because of the problems of cyberattacks, data loss and leakage, and big data. The
proposal then analyses how other countries had already addressed these concerns for their
citizens through legislation by looking at their regulation and the results of implementation. The
paper adjacently discusses the importance of American values of privacy as a fundamental right,
the free market, and protection from the private sector within a cybersecurity paradigm. From
this combined research, the paper yields a proposal of how the U.S. government should address
the situation through federal policy. The policy outlines cybersecurity measures to protect
information from cyberattacks and data loss and leakage, rights of American citizens that
organizations need to uphold, and the creation of a commission that provides resources and
education to domestic and foreign organizations.
ContributorsBrockman, Emily M. (Author) / Hollinger, Keith (Thesis director) / Walsh, Tatiana (Committee member) / Martin, Thomas (Committee member) / College of Integrative Sciences and Arts (Contributor) / Information Technology (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
In most bird species, females disperse prior to their first breeding attempt, while males remain close to the place they were hatched for their entire lives (Greenwood and Harvey (1982)). Explanations for such female bias in natal dispersal have focused on the potential benefits that males derive from knowing the local environment to establish territories, while females search for suitable mates (Greenwood (1980)). However, the variables shaping dispersal decisions appear more complex (Mabry et al. (2013), Végvári et al. (2018)). There are a number of different variables that could act as a driving force behind dispersal including the social mating system, food competition, inbreeding avoidance, predation, and others. Here, we investigate whether females are the dispersing sex in great-tailed grackles, which have a mating system where the males hold territories and the females choose which territory to place their nest in (Johnson et al. (2000)). We used genetic approaches to identify sex biases in the propensity to disperse. In the experiment, we found that the male grackles were less related to each other while the female grackles were more related to each other. Building on that, the average distance between closely related individuals of the male group was longer than the average distance of closely related females. But, the mantel correlograms for the males and females both lack a consistent trend. Overall, the results indicated suggest that the males are the dispersing sex while the females are potentially philopatric and that the average dispersal distance for the grackle is greater than 2000 meters, the size of the sampling range used in the experiment. These results will inform our long-term study on the relationship between behavioral flexibility and rapid geographic range expansion by elucidating which individuals are likely to experience similar conditions across their lives, and which are likely to face new conditions when they become breeders.
ContributorsSevchik, August L (Author) / Langergraber, Kevin (Thesis director) / Logan, Corina (Committee member) / College of Integrative Sciences and Arts (Contributor) / School of Molecular Sciences (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
In this paper, I describe the development of a unique approach to developing strategies for games in which success can only be measured by the final outcome of the game, preventing the use of heuristics. I created and evaluated evolutionary algorithms, applying them to develop strategies for tic-tac-toe. Strategies are comprised of neural networks with randomly initiated weights. A population of candidate strategies are created, each strategy competes individually against each other strategy, and evolutionary operators are applied to create subsequent generations of strategies. The set of strategies within a generation of the evolutionary algorithm forms a metagame that evolves as the algorithm progresses. Hypothesis testing shows that strategies produced by this approach significantly outperform a baseline of entirely random action, although they are still far from optimal gameplay.
ContributorsRodriguez, Julien Guillermo (Author) / Martin, Thomas (Thesis director) / Powers, Brian (Committee member) / College of Integrative Sciences and Arts (Contributor, Contributor) / Department of Information Systems (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05