Matching Items (122)
Description
Filtration for microfluidic sample-collection devices is desirable for sample selection, concentration, preprocessing, and downstream manipulation, but microfabricating the required sub-micrometer filtration structure is an elaborate process. This thesis presents a simple method to fabricate polydimethylsiloxane (PDMS) devices with an integrated membrane filter that will sample, lyse, and extract the DNA from microorganisms in aqueous environments. An off-the-shelf membrane filter disc was embedded in a PDMS layer and sequentially bound with other PDMS channel layers. No leakage was observed during filtration. This device was validated by concentrating a large amount of cyanobacterium Synechocystis in simulated sample water with consistent performance across devices. After accumulating sufficient biomass on the filter, a sequential electrochemical lysing process was performed by applying 5VDC across the filter. This device was further evaluated by delivering several samples of differing concentrations of cyanobacterium Synechocystis then quantifying the DNA using real-time PCR. Lastly, an environmental sample was run through the device and the amount of photosynthetic microorganisms present in the water was determined. The major breakthroughs in this design are low energy demand, cheap materials, simple design, straightforward fabrication, and robust performance, together enabling wide-utility of similar chip-based devices for field-deployable operations in environmental micro-biotechnology.
ContributorsLecluse, Aurelie (Author) / Meldrum, Deirdre (Thesis advisor) / Chao, Joseph (Thesis advisor) / Westerhoff, Paul (Committee member) / Arizona State University (Publisher)
Created2011
Description
Single cell phenotypic heterogeneity studies reveal more information about the pathogenesis process than conventional bulk methods. Furthermore, investigation of the individual cellular response mechanism during rapid environmental changes can only be achieved at single cell level. By enabling the study of cellular morphology, a single cell three-dimensional (3D) imaging system can be used to diagnose fatal diseases, such as cancer, at an early stage. One proven method, CellCT, accomplishes 3D imaging by rotating a single cell around a fixed axis. However, some existing cell rotating mechanisms require either intricate microfabrication, and some fail to provide a suitable environment for living cells. This thesis develops a microvorterx chamber that allows living cells to be rotated by hydrodynamic alone while facilitating imaging access. In this thesis work, 1) the new chamber design was developed through numerical simulation. Simulations revealed that in order to form a microvortex in the side chamber, the ratio of the chamber opening to the channel width must be smaller than one. After comparing different chamber designs, the trapezoidal side chamber was selected because it demonstrated controllable circulation and met the imaging requirements. Microvortex properties were not sensitive to the chambers with interface angles ranging from 0.32 to 0.64. A similar trend was observed when chamber heights were larger than chamber opening. 2) Micro-particle image velocimetry was used to characterize microvortices and validate simulation results. Agreement between experimentation and simulation confirmed that numerical simulation was an effective method for chamber design. 3) Finally, cell rotation experiments were performed in the trapezoidal side chamber. The experimental results demonstrated cell rotational rates ranging from 12 to 29 rpm for regular cells. With a volumetric flow rate of 0.5 µL/s, an irregular cell rotated at a mean rate of 97 ± 3 rpm. Rotational rates can be changed by altering inlet flow rates.
ContributorsZhang, Wenjie (Author) / Frakes, David (Thesis advisor) / Meldrum, Deirdre (Thesis advisor) / Chao, Shih-hui (Committee member) / Wang, Xiao (Committee member) / Arizona State University (Publisher)
Created2011
Description
This honors thesis utilizes smart home components and concepts from Dr. Burleson's Game as Life, Life as Game (GaLLaG) systems. The thesis focuses on an automated lifestyle, where individuals utilize technology, such as door sensors, appliance and lamp modules, and system notifications, to assist in daily activities. The findings from our efforts to date indicate that after weeks of observations, there is no evidence that automated lifestyles create more productive and healthy lifestyles and lead to overall satisfaction in life; however, there are certain design principles that would assist future home automation applications.
ContributorsRosales, Justin Bart (Author) / Burleson, Winslow (Thesis director) / Walker, Erin (Committee member) / Hekler, Eric (Committee member) / Civil, Environmental and Sustainable Engineering Programs (Contributor) / Barrett, The Honors College (Contributor)
Created2013-05
Description
Laboratory automation systems have seen a lot of technological advances in recent times. As a result, the software that is written for them are becoming increasingly sophisticated. Existing software architectures and standards are targeted to a wider domain of software development and need to be customized in order to use them for developing software for laboratory automation systems. This thesis proposes an architecture that is based on existing software architectural paradigms and is specifically tailored to developing software for a laboratory automation system. The architecture is based on fairly autonomous software components that can be distributed across multiple computers. The components in the architecture make use of asynchronous communication methodologies that are facilitated by passing messages between one another. The architecture can be used to develop software that is distributed, responsive and thread-safe. The thesis also proposes a framework that has been developed to implement the ideas proposed by the architecture. The framework is used to develop software that is scalable, distributed, responsive and thread-safe. The framework currently has components to control very commonly used laboratory automation devices such as mechanical stages, cameras, and also to do common laboratory automation functionalities such as imaging.
ContributorsKuppuswamy, Venkataramanan (Author) / Meldrum, Deirdre (Thesis advisor) / Collofello, James (Thesis advisor) / Sarjoughian, Hessam S. (Committee member) / Johnson, Roger (Committee member) / Arizona State University (Publisher)
Created2012
Description
Single cell analysis has become increasingly important in understanding disease onset, progression, treatment and prognosis, especially when applied to cancer where cellular responses are highly heterogeneous. Through the advent of single cell computerized tomography (Cell-CT), researchers and clinicians now have the ability to obtain high resolution three-dimensional (3D) reconstructions of single cells. Yet to date, no live-cell compatible version of the technology exists. In this thesis, a microfluidic chip with the ability to rotate live single cells in hydrodynamic microvortices about an axis parallel to the optical focal plane has been demonstrated. The chip utilizes a novel 3D microchamber design arranged beneath a main channel creating flow detachment into the chamber, producing recirculating flow conditions. Single cells are flowed through the main channel, held in the center of the microvortex by an optical trap, and rotated by the forces induced by the recirculating fluid flow. Computational fluid dynamics (CFD) was employed to optimize the geometry of the microchamber. Two methods for the fabrication of the 3D microchamber were devised: anisotropic etching of silicon and backside diffuser photolithography (BDPL). First, the optimization of the silicon etching conditions was demonstrated through design of experiment (DOE). In addition, a non-conventional method of soft-lithography was demonstrated which incorporates the use of two positive molds, one of the main channel and the other of the microchambers, compressed together during replication to produce a single ultra-thin (<200 µm) negative used for device assembly. Second, methods for using thick negative photoresists such as SU-8 with BDPL have been developed which include a new simple and effective method for promoting the adhesion of SU-8 to glass. An assembly method that bonds two individual ultra-thin (<100 µm) replications of the channel and the microfeatures has also been demonstrated. Finally, a pressure driven pumping system with nanoliter per minute flow rate regulation, sub-second response times, and < 3% flow variability has been designed and characterized. The fabrication and assembly of this device is inexpensive and utilizes simple variants of conventional microfluidic fabrication techniques, making it easily accessible to the single cell analysis community.
ContributorsMyers, Jakrey R (Author) / Meldrum, Deirdre (Thesis advisor) / Johnson, Roger (Committee member) / Frakes, David (Committee member) / Arizona State University (Publisher)
Created2012
Description
Violence in schools occurs throughout America, prevalent to the point of daily happenstance. The epidemic of violence in our society is in sore need of healing efforts. Ending Bullying with Multiple Architectures focuses on the violence of bullying in young children and adolescents, in an effort to mitigate bullying at a critical age, before it transcends into their adult behavior. Bullying begins in elementary schools, a time when our minds are extremely impressionable and our behavioral habits take birth. Bullying may happen for a certain segment of a person's life, but the effects transcend a person's entire life. People who bully may follow a familial cycle of bullying and people who are bullied may become bullies and start a new cycle. With bullying and aggressive behavior increasing exponentially in schools, our society is growing up in a place where it is acceptable to react aggressively to stressful or undesirable situations. Today, violence in our society infiltrates every aspect of our lives, from road rage, to grocery store quarrels, to family ties breaking, to gun violence in school and public spaces. Unplanned acts of violence occur in "spur of the moments". Is our society so impatient, aggressive, antagonistic, individualistic, and isolated because we have been conditioned as human beings to behave this way? Did we miss our chance to work cordially as a community, peacefully and patiently, because we put progress and productivity in front of community and collaboration? How can architecture slow you down, keep you aware of your surroundings and facilitate collaboration and getting along? Why do we accept abrupt anger and violence, and how can architecture create, improve or encourage positive behavioral habits in our impressionable young minds? Ending Bullying with Multiple Architectures translates existing bullying strategies (social architecture) into physical architectural intervention, in an effort to mitigate bullying at the critical age when behavioral habits take birth. This project challenges efficiency based design in order to complement the human experience. By creating healthier spaces that foster wholeness, we can heal violence at this critical age, and thus hopefully reduce future societal violence as a whole.
ContributorsRaghani, Divya Nikita (Author) / Shraiky, James (Thesis director) / Hejduk, Renata (Committee member) / Civil, Environmental and Sustainable Engineering Programs (Contributor) / Barrett, The Honors College (Contributor) / The Design School (Contributor) / School of Social and Behavioral Sciences (Contributor)
Created2015-05
Description
Engineering education has long sought to incorporate greater diversity into engineering programs to prepare the profession to meet the engineering challenges of society. Increasing or retaining the conative diversity of engineering programs may help extend other kinds of diversity in the profession as well (Marburger, 2004). One measure of conation is the Kolbe ATM index.
Kolbe ATM is an index developed by Kathy Kolbe to measure the conative traits on an individual. The index assigns each individual a value in four categories, or Action Modes, that indicates their level of insistence on a scale of 1 to 10 in that Action Mode (Kolbe, 2004). The four Action Modes are:
• Fact Finder – handling of information or facts
• Follow Thru – need to pattern or organize
• Quick Start – management of risk or uncertainty
• Implementor – interaction with space or tangibles
The Kolbe A (TM) index assigns each individual a value that indicates their level of insistence with 1-3 representing resistant, preventing problems in a particular Action Mode; 4-6 indicating accommodation, flexibility in a particular Action Mode; and 7-10 indicating insistence in an Action Mode, initiating solutions in that Action Mode (Kolbe, 2004).
To promote retention of conative diversity, this study examines conative diversity in two engineering student populations, a predominately freshmen population at Chandler Gilbert Community College and a predominately junior population at Arizona State University. Students in both population took a survey that asked them to self-report their GPA, satisfaction with required courses in their major, Kolbe ATM conative index, and how much their conative traits help them in each of the classes on the survey. The classes in the survey included two junior level classes at ASU, Engineering Business Practices and Structural Analysis; as well as four freshmen engineering classes, Physics Lecture, Physics Lab, English Composition, and Calculus I.
This study finds that student satisfaction has no meaningful correlation with student GPA.
The study also finds that engineering programs have a dearth of resistant Fact Finders from the freshmen level on and losses resistant Follow Thrus and insistent Quick Starts as time progresses. Students whose conative indices align well with the structure of the engineering program tend to consider their conative traits helpful to them in their engineering studies. Students whose conative indices misalign with the structure of the program report that they consider their strengths less helpful to them in their engineering studies.
This study recommends further research into the relationship between satisfaction with major and conation and into perceived helpfulness of conative traits by students. Educators should continue to use Kolbe A (TM) in the classroom and perform further research on the impacts of conation on diversity in engineering programs.
Kolbe ATM is an index developed by Kathy Kolbe to measure the conative traits on an individual. The index assigns each individual a value in four categories, or Action Modes, that indicates their level of insistence on a scale of 1 to 10 in that Action Mode (Kolbe, 2004). The four Action Modes are:
• Fact Finder – handling of information or facts
• Follow Thru – need to pattern or organize
• Quick Start – management of risk or uncertainty
• Implementor – interaction with space or tangibles
The Kolbe A (TM) index assigns each individual a value that indicates their level of insistence with 1-3 representing resistant, preventing problems in a particular Action Mode; 4-6 indicating accommodation, flexibility in a particular Action Mode; and 7-10 indicating insistence in an Action Mode, initiating solutions in that Action Mode (Kolbe, 2004).
To promote retention of conative diversity, this study examines conative diversity in two engineering student populations, a predominately freshmen population at Chandler Gilbert Community College and a predominately junior population at Arizona State University. Students in both population took a survey that asked them to self-report their GPA, satisfaction with required courses in their major, Kolbe ATM conative index, and how much their conative traits help them in each of the classes on the survey. The classes in the survey included two junior level classes at ASU, Engineering Business Practices and Structural Analysis; as well as four freshmen engineering classes, Physics Lecture, Physics Lab, English Composition, and Calculus I.
This study finds that student satisfaction has no meaningful correlation with student GPA.
The study also finds that engineering programs have a dearth of resistant Fact Finders from the freshmen level on and losses resistant Follow Thrus and insistent Quick Starts as time progresses. Students whose conative indices align well with the structure of the engineering program tend to consider their conative traits helpful to them in their engineering studies. Students whose conative indices misalign with the structure of the program report that they consider their strengths less helpful to them in their engineering studies.
This study recommends further research into the relationship between satisfaction with major and conation and into perceived helpfulness of conative traits by students. Educators should continue to use Kolbe A (TM) in the classroom and perform further research on the impacts of conation on diversity in engineering programs.
ContributorsSmith, Logan Farren (Author) / Seager, Thomas P. (Thesis director) / Adams, Elizabeth A. (Committee member) / Civil, Environmental and Sustainable Engineering Programs (Contributor) / Barrett, The Honors College (Contributor)
Created2015-05
Description
Utilizing an urban canopy model (UCM) developed by Zhihua Wang, Ph.D. for a research study conducted for the National Asphalt Pavement Association (NAPA), several scenarios were run in order to determine the impact on the mitigation of the urban heat island (UHI) effect. These scenarios included various roof albedo, wall albedo, ground albedo, a combination of all three albedos, roof emissivity, wall emissivity, ground emissivity, a combination of all three emissivities, and normalized building height as independent variables. Dependent variables included canyon air temperature, effective ground temperature, effective roof temperature, effective wall temperature, and sensible heat flux. It was found that emissivity does play a part in reducing the different dependent variables; however, typically emissivity values are already within a preferred range that not much can be done with them. Normalized building height has a minor impact but the impact that it does have upon the different variables is lessened with lower values of the normalized building height. Increasing the wall albedo decreased the canyon air temperature and the effective wall temperature the most compared to the other variables when considering expenses. An increase in roof albedo reduced effective roof temperature and sensible heat flux the most when taking into consideration the cost of changing the albedo of the surface. Larger values of ground albedo helped to reduce the effective ground temperature more than the other variables considered when a budget is necessary.
ContributorsHousenga, Hannah Eileen (Author) / Kaloush, Kamil (Thesis director) / Wang, Zhihua (Committee member) / Civil, Environmental and Sustainable Engineering Programs (Contributor) / Barrett, The Honors College (Contributor)
Created2015-05
Description
"The Legal Adventures of Frankie and Rosie" is a creative project that explores the nontraditional format of comics to express creative nonfiction. The project is a set of 30 independent comics that focuses on two primary college-going students who are based off of the authors. The characters, Frankie and Rosie narrate their stories through dialogue. The authors use this narrative model to archive their college experience at ASU. Representing creative nonfiction through comics yields an amalgamated format that can be challenging for both the writers to produce as well as for the readers to consume. Ultimately, the project serves as an attempt to test whether or not the comic medium can stand by itself as an appropriate format to express creative nonfictional narratives without becoming a diluted combination of its purer predecessors.
ContributorsBennewitz, Dana (Co-author) / Ray, Payel (Co-author) / Martin, Thomas (Thesis director) / Scott Lynch, Jacquelyn (Committee member) / Civil, Environmental and Sustainable Engineering Programs (Contributor) / Barrett, The Honors College (Contributor) / School of Geographical Sciences and Urban Planning (Contributor) / Electrical Engineering Program (Contributor) / School of Social Transformation (Contributor) / School of Mathematical and Statistical Sciences (Contributor)
Created2015-05
Description
This dissertation details an attempt to experimentally evaluate the Giroud et al. (1995) concentration factors for geomembranes loaded in tension perpendicular to a seam by laboratory measurement. Field observations of the performance of geomembrane liner systems indicates that tears occur at average strains well below the yield criteria. These observations have been attributed, in part, to localized strain concentrations in the geomembrane loaded in tension in a direction perpendicular to the seam. Giroud et al. (1995) has presented theoretical strain concentration factors for geomembrane seams loaded in tension when the seam is perpendicular to the applied tensile strain. However, these factors have never been verified. This dissertation was prepared in fulfillment of the requirements for graduation from Barrett, the Honors College at Arizona State University. The work described herein was sponsored by the National Science Foundation as a part of a larger research project entitled "NEESR: Performance Based Design of Geomembrane Liner Systems Subject to Extreme Loading." The work is motivated by geomembrane tears observed at the Chiquita Canyon landfill following the 1994 Northridge earthquake. Numerical analysis of the strains in the Chiquita Canyon landfill liner induced by the earthquake indicated that the tensile strains, were well below the yield strain of the geomembrane material. In order to explain why the membrane did fail, strain concentration factors due to bending at seams perpendicular to the load in the model proposed by Giroud et al. (1995) had to be applied to the geomembrane (Arab, 2011). Due to the localized nature of seam strain concentrations, digital image correlation (DIC) was used. The high resolution attained with DIC had a sufficient resolution to capture the localized strain concentrations. High density polyethylene (HDPE) geomembrane samples prepared by a leading geomembrane manufacturer were used in the testing described herein. The samples included both extrusion fillet and dual hot wedge fusion seams. The samples were loaded in tension in a standard triaxial test apparatus. to the seams in the samples including both extrusion fillet and dual hot wedge seams. DIC was used to capture the deformation field and strain fields were subsequently created by computer analysis.
ContributorsAndresen, Jake Austin (Author) / Kavazanjian, Edward (Thesis director) / Gutierrez, Angel (Committee member) / Civil, Environmental and Sustainable Engineering Programs (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05