Matching Items (147)
Description
Infections caused by the Hepatitis C Virus (HCV) are very common worldwide, affecting up to 3% of the population. Chronic infection of HCV may develop into liver cirrhosis and liver cancer which is among the top five of the most common cancers. Therefore, vaccines against HCV are under intense study in order to prevent HCV from harming people's health. The envelope protein 2 (E2) of HCV is thought to be a promising vaccine candidate because it can directly bind to a human cell receptor and plays a role in viral entry. However, the E2 protein production in cells is inefficient due to its complicated matured structure. Folding of E2 in the endoplasmic reticulum (ER) is often error-prone, resulting in production of aggregates and misfolded proteins. These incorrect forms of E2 are not functional because they are not able to bind to human cells and stimulate antibody response to inhibit this binding. This study is aimed to overcome the difficulties of HCV E2 production in plant system. Protein folding in the ER requires great assistance from molecular chaperones. Thus, in this study, two molecular chaperones in the ER, calreticulin and calnexin, were transiently overexpressed in plant leaves in order to facilitate E2 folding and production. Both of them showed benefits in increasing the yield of E2 and improving the quality of E2. In addition, poorly folded E2 accumulated in the ER may cause stress in the ER and trigger transcriptional activation of ER molecular chaperones. Therefore, a transcription factor involved in this pathway, named bZIP60, was also overexpressed in plant leaves, aiming at up-regulating a major family of molecular chaperones called BiP to assist protein folding. However, our results showed that BiP mRNA levels were not up-regulated by bZIP60, but they increased in response to E2 expression. The Western blot analysis also showed that overexpression of bZIP60 had a small effect on promoting E2 folding. Overall, this study suggested that increasing the level of specific ER molecular chaperones was an effective way to promote HCV E2 protein production and maturation.
ContributorsHong, Fan (Author) / Mason, Hugh (Thesis advisor) / Gaxiola, Roberto (Committee member) / Chang, Yung (Committee member) / Chen, Qiang (Committee member) / Arizona State University (Publisher)
Created2011
Description
My thesis project, "Reforming the NCAA: How the governing body of intercollegiate athletics should handle student-athletes into the future," sets out to create a working blueprint for how the NCAA should handle the relationship between student-athletes, universities, and the NCAA from this point forward. The NCAA has come under fire in the past 10 years for its failure to meet modern social constructs and provide student-athletes with the resources necessary to achieving a successful educational and athletic experience, and through my thesis, I formulated reforms the NCAA can adopt and enact to respond to the growing issues within intercollegiate athletics. I began the process last spring with my thesis director as we selected a topic together, and I researched a variety of topics relating to current NCAA issues throughout the summer and fall. In the fall, I outlined sources I hoped to interview, and I conducted interviews over winter break. I spent the first two months of 2015 writing and refining my thesis, and through March, I created a PowerPoint presentation I used to defend my thesis project. During the process, I met with my thesis director at critical junctures to discuss the direction of the project and to determine how to find a delicate balance between creating attainable goals for reform and overstepping my boundaries. After a successful thesis defense, I made small revisions to the thesis and had my project re-approved by my director and second reader. In the coming weeks after submitting my thesis, I plan on exploring the possibility of having my thesis published and received critiques from those in the industry who follow collegiate athletics closely.
ContributorsCrowley, Kerry O Shea (Author) / McGuire, Tim (Thesis director) / Kurland, Brett (Committee member) / College of Letters and Sciences (Contributor) / Walter Cronkite School of Journalism and Mass Communication (Contributor) / Barrett, The Honors College (Contributor)
Created2015-05
Description
Surface plasmon resonance (SPR) has emerged as a popular technique for elucidating subtle signals from biological events in a label-free, high throughput environment. The efficacy of conventional SPR sensors, whose signals are mass-sensitive, diminishes rapidly with the size of the observed target molecules. The following work advances the current SPR sensor paradigm for the purpose of small molecule detection. The detection limits of two orthogonal components of SPR measurement are targeted: speed and sensitivity. In the context of this report, speed refers to the dynamic range of measured kinetic rate constants, while sensitivity refers to the target molecule mass limitation of conventional SPR measurement. A simple device for high-speed microfluidic delivery of liquid samples to a sensor surface is presented to address the temporal limitations of conventional SPR measurement. The time scale of buffer/sample switching is on the order of milliseconds, thereby minimizing the opportunity for sample plug dispersion. The high rates of mass transport to and from the central microfluidic sensing region allow for SPR-based kinetic analysis of binding events with dissociation rate constants (kd) up to 130 s-1. The required sample volume is only 1 μL, allowing for minimal sample consumption during high-speed kinetic binding measurement. Charge-based detection of small molecules is demonstrated by plasmonic-based electrochemical impedance microscopy (P-EIM). The dependence of surface plasmon resonance (SPR) on surface charge density is used to detect small molecules (60-120 Da) printed on a dextran-modified sensor surface. The SPR response to an applied ac potential is a function of the surface charge density. This optical signal is comprised of a dc and an ac component, and is measured with high spatial resolution. The amplitude and phase of local surface impedance is provided by the ac component. The phase signal of the small molecules is a function of their charge status, which is manipulated by the pH of a solution. This technique is used to detect and distinguish small molecules based on their charge status, thereby circumventing the mass limitation (~100 Da) of conventional SPR measurement.
ContributorsMacGriff, Christopher Assiff (Author) / Tao, Nongjian (Thesis advisor) / Wang, Shaopeng (Committee member) / LaBaer, Joshua (Committee member) / Chae, Junseok (Committee member) / Arizona State University (Publisher)
Created2013
Description
This dissertation investigates the condition of skeletal muscle insulin resistance using bioinformatics and computational biology approaches. Drawing from several studies and numerous data sources, I have attempted to uncover molecular mechanisms at multiple levels. From the detailed atomistic simulations of a single protein, to datamining approaches applied at the systems biology level, I provide new targets to explore for the research community. Furthermore I present a new online web resource that unifies various bioinformatics databases to enable discovery of relevant features in 3D protein structures.
ContributorsMielke, Clinton (Author) / Mandarino, Lawrence (Committee member) / LaBaer, Joshua (Committee member) / Magee, D. Mitchell (Committee member) / Dinu, Valentin (Committee member) / Willis, Wayne (Committee member) / Arizona State University (Publisher)
Created2013
Description
Recombinant protein expression is essential to biotechnology and molecular medicine, but facile methods for obtaining significant quantities of folded and functional protein in mammalian cell culture have been lacking. Here I describe a novel 37-nucleotide in vitro selected sequence that promotes unusually high transgene expression in a vaccinia driven cytoplasmic expression system. Vectors carrying this sequence in a monocistronic reporter plasmid produce >1,000-fold more protein than equivalent vectors with conventional vaccinia promoters. Initial mechanistic studies indicate that high protein expression results from dual activity that impacts both transcription and translation. I suggest that this motif represents a powerful new tool in vaccinia-based protein expression and vaccine development technology.
ContributorsFlores, Julia Anne (Author) / Chaput, John C (Thesis advisor) / Jacobs, Bertram (Committee member) / LaBaer, Joshua (Committee member) / Arizona State University (Publisher)
Created2012
Description
Salmonella enterica is a gastrointestinal (GI) pathogen that can cause systemic diseases. It invades the host through the GI tract and can induce powerful immune responses in addition to disease. Thus, it is considered as a promising candidate to use as oral live vaccine vectors. Scientists have been making great efforts to get a properly attenuated Salmonella vaccine strain for a long time, but could not achieve a balance between attenuation and immunogenicity. So the regulated delayed attenuation/lysis Salmonella vaccine vectors were proposed as a design to seek this balance. The research work is progressing steadily, but more improvements need to be made. As one of the possible improvements, the cyclic adenosine monophosphate (cAMP) -independent cAMP receptor protein (Crp*) is expected to protect the Crp-dependent crucial regulator, araC PBAD, in these vaccine designs from interference by glucose, which decreases synthesis of cAMP, and enhance the colonizing ability by and immunogenicity of the vaccine strains. In this study, the cAMP-independent crp gene mutation, crp-70, with or without araC PBAD promoter cassette, was introduced into existing Salmonella vaccine strains. Then the plasmid stability, growth rate, resistance to catabolite repression, colonizing ability, immunogenicity and protection to challenge of these new strains were compared with wild-type crp or araC PBAD crp strains using western blots, enzyme-linked immunosorbent assays (ELISA) and animal studies, so as to evaluate the effects of the crp-70 mutation on the vaccine strains. The performances of the crp-70 strains in some aspects were closed to or even exceeded the crp+ strains, but generally they did not exhibit the expected advantages compared to their wild-type parents. Crp-70 rescued the expression of araC PBAD fur from catabolite repression. The strain harboring araC PBAD crp-70 was severely affected by its slow growth, and its colonizing ability and immunogenicity was much weaker than the other strains. The Pcrp crp-70 strain showed relatively good ability in colonization and immune stimulation. Both the araC PBAD crp-70 and the Pcrp crp-70 strains could provide certain levels of protection against the challenge with virulent pneumococci, which were a little lower than for the crp+ strains.
ContributorsShao, Shihuan (Author) / Curtiss, Roy (Thesis advisor) / Arizona State University (Publisher)
Created2012
Description
The relationship between mental illness (or "madness," as Socrates labeled psychosis, as well as a wide range of states of thought and emotion [Jamison, 1993]) and creativity has been noted since before the times of Socrates. The questions that this noted relationship pose are significant. Famous authors, poets, artists, and musicians who suffered from various forms of mental illness are littered throughout history. Edgar Allen Poe himself noted, "Men have called me mad but the question is not yet settled, whether madness is or is not the loftiest intelligence \u2014 whether much that is glorious \u2014 whether all that is profound \u2014 does not spring from disease of thought \u2014 from moods of mind exalted at the expense of the general intellect." Only in recent years have researchers begun to classify these disorders of past artists using modern diagnostic measures. While the concept of a relationship between creativity and mental illness is fascinating in and of itself, understanding the implications of a possible correlation is essential to the care and treatment that individuals with the diseases are given. If, indeed, creativity is linked with affective disorders, what does this mean for the treatment of these disorders? By eliminating the symptoms of mood disorders, are we also inadvertently eliminating creativity? It is important to acknowledge the possibility that mental illnesses like bipolar disorder include benefits to those afflicted. How can we treat the negative symptoms of mood disorders while enhancing the positive symptoms? In order to conclude that there is a link between creativity and mood disorders, it is first necessary to reexamine and reestablish the processes by which mood disorders are diagnosed. Although currently diagnosed on a categorical scale \u2014 meaning the patient is diagnosed under the black-and-white category of manic or depressive at a given point in time \u2014 this paper will argue that mood disorders can be better diagnosed and treated on a continuum of mood states. Furthermore, by viewing mood disorders on such a continuum, writers like Anne Sexton and Sylvia Plath, whose diagnoses are debated by historians and professionals, can be established as having a diagnosable affective disorder, since both experienced symptoms of depression and mania, if not in the precise timing and manner proscribed by the Diagnostic Manual. Finally, by determining these writers' diagnoses, a relationship can be explored between their creative states and their mood states.
ContributorsKugler, Danica Eileen (Author) / Montesano, Mark (Thesis director) / Holbo, Christine (Committee member) / Barrett, The Honors College (Contributor) / College of Letters and Sciences (Contributor) / Department of Psychology (Contributor) / Department of English (Contributor)
Created2015-05
Description
This full-length, two-act play explores the way loss brings together and tears apart two families in the United States, a lower-middle class Mexican family and a relatively wealthy white family. Throughout the play we explore family dynamics, culture, and how we all ultimately cope with navigating a complex and often devastating world. While this thesis project has completed the honors requirements, the play itself is still under construction. The version you see here is a final thesis project, but not a final product.
ContributorsBilker, Molly Alexandra (Author) / King de Ramirez, Carmen (Thesis director) / Stephens, Mary (Committee member) / Barrett, The Honors College (Contributor) / College of Letters and Sciences (Contributor) / Walter Cronkite School of Journalism and Mass Communication (Contributor)
Created2015-05
Description
Armenia is a radiant and unique country, known primarily for its vast history, delicious food, and traditional folklore dance. Although Armenia was constantly ravaged by invading armies, the nation was strong enough to retain its own identity and culture. The rich dance heritage remained a living tradition into the 20th century, when the Turks tried to destroy the dance heritage and left fragments of the dances. Even though many of these fragments were lost due to urbanization, the dance techniques are still continuously studied by scholars and people all around the world. For my creative project, I chose to perform an Armenian Folk Dance, as dance is an immense part of Armenian’s lives, and is an essential part of the Armenian cultural heritage. Dance is one of the primary ways by which one can observe and gain understanding of the Armenian people, their identity and culture. Dance is a way for Armenians to express themselves, as it is a form of art which combines elements of their rich culture, event-filled past, and their spiritual nature. The folklore dance has always sustained a leading role throughout Armenian history, as it runs through the blood of Armenians, being passed down from generation to generation. The Armenian people have survived and endured various historical challenges, but they have been able to preserve their unique dance elements and forms. To this day, Armenians continue to use dance to keep their culture and identity alive. It is a way to express freedom, and celebrate that even though we as a nation have faced many hardships, us Armenians have survived, and will always continue to survive with our faith, passion, determination, and strong will.
ContributorsKaprelian, Daniella Mary (Author) / McILwraith, Heide (Thesis director) / Alford, Eddie (Committee member) / Barrett, The Honors College (Contributor) / College of Letters and Sciences (Contributor)
Created2015-05
Evaluation of Calcium and Sodium Salts on Seed Germination and Early Growth of Leucaena leucocephala
Description
This study was conducted to determine the effect of various sodium and calcium-based saline treatments on the germination and early growth of Leucaena leucocephala. The evaluation of calcium-based salts has never been studied for its effects on Leucaena leucocephala. This experiment utilized 5 increasing concentrations of sodium chloride, calcium chloride, and a 1:1 ratio of NaCl: CaCl2. The growth and germination of these seeds were recorded over the course of 11 days. Two trials of this experiment were run- the first using seeds collected from ASU's Polytechnic campus and the second using seeds collected from roadside landscaping. The results of both trials indicated that germination and early growth are affected by the different saline treatments. For trial one, the seeds germinated best under treatments involving either 0.0125 or 0.025 M CaCl2 or NaCl: CaCl2. For trial two, the seeds germinated best under treatments involving either 0.025 or 0.05 M CaCl2 or NaCl: CaCl2. Thus, seeds from Leucaena leucocephala are best germinated under saline treatments consisting of either just calcium chloride or a 1:1 mixture of sodium chloride and calcium chloride.
ContributorsFiorentin, Giovanna Marie (Author) / Martin, Chris (Thesis director) / Stutz, Jean (Committee member) / Barrett, The Honors College (Contributor) / College of Letters and Sciences (Contributor)
Created2015-05