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- Creators: School of Life Sciences
Description
I am evaluating the genomic basis of a model of heat tolerance in which organisms succumb to warming when their demand for oxygen exceeds their supply. This model predicts that tolerance of hypoxia should correlate genetically with tolerance of heat. To evaluate this prediction, I tested heat and hypoxia tolerance in several genetic lines of Drosophila melanogaster. I hypothesized that genotypes that can fly better at high temperatures are also able to fly well at hypoxia. Genotypes from the Drosophila Genetic Reference Panel (DGRP) were assessed for flight at hypoxia and normal temperature (12% O2 and 25°C) as well as normoxia and high temperature (21% O2 and 39°C). After testing 66 lines from the DGRP, the oxygen- and capacity-limited thermal tolerance theory is supported; hypoxia-resistant lines are more likely to be heat-resistant. This supports previous research, which suggested an interaction between the tolerance of the two environmental variables. I used this data to perform a genome-wide association study to find specific single-nucleotide polymorphisms associated with heat tolerance and hypoxia tolerance but found no specific genomic markers. Understanding factors that limit an organism’s stress tolerance as well as the regions of the genome that dictate this phenotype should enable us to predict how organisms may respond to the growing threat of climate change.
ContributorsFredette-Roman, Jacob Daniel (Author) / Angilletta, Michael (Thesis director) / VandenBrooks, John (Committee member) / Youngblood, Jacob (Committee member) / School of Life Sciences (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
The United States is one of the countries with the highest rates of maternal mortality in the world, with significantly higher rates than other developed countries. Maternal mortality is defined as the death of women who are pregnant or recently gave birth. Many studies suggest that those deaths are preventable and result from a lack of quality care and unnecessary medical interventions. It is notable that there are specific populations, which I describe as “vulnerable,” that are at increased risk of maternal mortality, including women of color, women in poverty, and women with low socioeconomic statuses. The United States also has lower rates of midwife-assisted births than other developed countries, which suggests that there is an association between adverse birth outcomes, such as maternal mortality, and midwifery care. In my thesis, I investigate that association and answer the question: Do midwives lower rates of adverse birth outcomes in vulnerable populations? I describe the history of midwifery in the United States and analyze the factors that lead to lower adverse birth outcomes in midwife-attended births. I then suggest methods for integrating midwives into the United States healthcare system, which I suggest will lower rates of maternal mortality.
ContributorsOreilly, Megan Ashley (Author) / Maienschein, Jane (Thesis director) / Abboud, Carolina (Committee member) / Ellsworth Bowers, Esther (Committee member) / School of Life Sciences (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
The bulk of this thesis is the translation itself—of Episodes 8-10—with timed subtitles. However, I also wanted to provide commentary on a few issues of interest that I came across during the translation. Conveying meaning from one language to the other does not simply involve translating the denotations of each word, but also the social context, humor, tone, and other aspects surrounding the situation. Whether words are used individually to interject, or come together to form unique idioms or phrases, the challenge presented to the translator is the choices: what words do I select to best convey not only the dictionary sense, but also the contextual meaning? This is especially difficult in wordplay-type situations. If there’s no perfect option, can I find a cultural analog that differs from the original denotation, but preserves the intention of the words? Otherwise, do I find the original important enough that it is worth educating the reader in order to preserve it—or, do I see no other translation option but to educate as a last resort? These are all choices that I had to make, and in this thesis I will be discussing the most interesting situations that came up, and my choices in dealing with them.
ContributorsLiong, Katerina Vakana (Author) / Tueller, Michael A. (Thesis director) / Printezis, Antonios (Committee member) / School of Life Sciences (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
The objective of this study was to compare the effectiveness of a newly developed DJO Global cervical collar with the previously studied Össur Americas Miami J collar in restricting cervical spine movement and reducing tissue interface pressure. 3D kinematic data were obtained for twelve healthy participants volunteers (6 female, 6 male) using a 10 camera infrared motion capture system (Motion Analysis Corp.). Cervical range of motion (CROM) in each plane was calculated as the angle between the head and thorax rigid-body axes. CROM was calculated using custom-written Matlab (MathWorks, Natick, MA) scripts. Tissue interface pressure (TIP) was measured between the head and the collar with three flexible pressure sensor pads over the anterior mandibles and occiput. The distribution of interface pressures was obtained in both the seated and supine positions. Both collars significantly restricted range of motion in all movement directions (p < 0.001) compared to no collar. There were no statistically significant differences in restrictiveness nor tissue interface pressures between the collars. Both collars exhibited similar CROM restriction in all planes and similar interface pressures in both positions. The newly developed DJO collar properly functioned as it markedly restricted spinal movement and produced low contact pressures. The Miami J collar has long been scientifically recognized as an effective collar; however, our data shows that the latest DJO collar was able to exhibit comparable contact pressures and decreases in cervical motion. As manufacturers produce improved collar designs, continued scientific testing should be executed in search of a collar capable of enhanced CROM restriction and the diminution of TIP.
ContributorsAraghi, Kasra (Author) / Gile, Gillian (Thesis director) / McCamley, John (Committee member) / Jacofsky, Marc (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
Malignant Pleural Mesothelioma is a type of lung cancer usually discovered at an advanced stage at which point there is no cure. Six primary MPM cell lines were used to conduct in vitro research to make conclusions about specific gene mutations associated with Mesothelioma. DNA exome sequencing, a time efficient and inexpensive technique, was used for identifying specific DNA mutations. Computational analysis of exome sequencing data was used to make conclusions about copy number variation among common MPM genes. Results show a CDKN2A gene heterozygous deletion in Meso24 cell line. This data is validated by a previous CRISPR-Cas9 outgrowth screen for Meso24 where the knocked-out gene caused increased Meso24 growth.
ContributorsKrdi, Ghena (Author) / Plaisier, Christopher (Thesis director) / Wilson, Melissa (Committee member) / School of Life Sciences (Contributor) / Hugh Downs School of Human Communication (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
Yellow-bellied marmots (Marmota flavivent) are semi-fossorial ground-dwelling sciurid rodents native to the western United States. They are facultatively social and live in colonies that may contain over 50 individuals. Marmot populations are well studied in terms of their diet, life cycle, distribution, and behavior, however, knowledge about viruses associated with marmots is very limited. In this study we aim to identify DNA viruses by non-invasive sampling of their feces. Viral DNA was extracted from fecal material of 35 individual marmots collected in Colorado and subsequently submitted to rolling circle amplification for circular molecule enrichment. Using a viral metagenomics approach which included high-throughput sequencing and verification of viral genomes using PCR, cloning and sequencing, a diverse group of single-stranded (ss) DNA viruses were identified. Diverse ssDNA viruses were identified that belong to two established families, Genomoviridae (n=7) and Anelloviridae (n=1) and several others that belong to unclassified circular replication associated encoding single-stranded (CRESS) DNA virus groups (n=19). There were also circular DNA molecules extracted (n=4) that appear to encode one viral-like gene and are composed of <1545 nt. The viruses that belonged to the family Genomoviridae clustered with those in the Gemycircularvirus genus. The genomoviruses were extracted from 6 samples. These clustered with gemycircularvirus extracted from arachnids and feces. The anellovirus, extracted from one sample, identified here has a genome sequence that is most similar to those from other rodent species, lagomorphs, and mosquitos. The CRESS viruses identified here were extracted from 9 samples and are novel and cluster with others identified from avian species. This study gives a snapshot of viruses associated with marmots based on fecal sampling.
ContributorsKhalifeh, Anthony (Author) / Varsani, Arvind (Thesis director) / Kraberger, Simona (Committee member) / Dolby, Greer (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
Multiple sclerosis is currently deemed the most common autoimmune disease. By definition, multiple sclerosis, known more commonly as MS, involves an immune-mediated process in which an abnormal response of the body’s immune system is directed against the central nervous system (“Definition of MS,” n.d.). Common treatment protocols call for daily, monthly, or yearly disease-modifying medications. These drugs are taken indefinitely to stop the spread and appearance of new lesions, improve symptoms, and offer relief to the afflicted individuals. The necessity for patients to take these basic medical treatments is paramount, however, it should not be overlooked to make lifestyle changes as well. The purpose of this paper is to give a detailed understanding of multiple sclerosis, its etiology evolution, and medical advancements, while emphasizing the necessary transitions which must be made from a nutritional and lifestyle management standpoint. A brief focus will be placed on sleep, exercise, and stress management, with an emphasis on nutrition.
ContributorsDeets, Breanna L (Author) / Levinson, Simin (Thesis director) / Grant, Shauna (Committee member) / Department of Management and Entrepreneurship (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
In “The Trouble of Wilderness,” William Cronon (1995) states the concept of wilderness, historically, is based on the romanticized ideal of the “untamed” frontier that influenced the American expansion ideals in the late 1800s and early 1900s, including the initial conservation movement. This idea of wilderness is defined by “empty” lands that needed to be utilized by the civilized Anglo-Americans, or lands that needed to be preserved from human alterations. Wilderness was separate from humans and, therefore, was also thought to be land that had been unaltered by human touch. The disappearing frontier was being turned into farmlands and civilization, so the Anglo-Americans, the ones who culturally viewed undeveloped land as a place for recreation, wished to save the ‘wilderness’ that was not yet being used. But as will be discussed it was in fact being used just not by the Anglo-Americans. This wilderness that they were trying to preserve became the national parks, such as Yellowstone and Yosemite. Under this rationale, Indigenous peoples were forced off the land to create the illusion of these places fitting this romanticized idea of wilderness. This essay examines the national parks in context of this concept of wilderness being free from humans and how national parks rationalized the removal of Indigenous people from these “wild” lands by using this concept of wilderness. Specifically, it uses the history of Yellowstone and Yosemite parks, which are some of the first parks to enter the National Park System, as sites of understanding how the idea wilderness was conceptualized by the American government during the late 1800s as places that are separate from humans. This essay argues that these ideals are based on racist and xenophobic approaches that the early United States government used in regards to relationships with Indigenous people. To discuss these ideas, this paper will examine the language used in early government documents regarding the policies of the national parks along with art and writings from this time period to show how the public and government viewed these national parks and the Indigenous people in the surrounding areas. Particularly, this paper will consider the original documents that established the national parks and the language that was used in these documents. It will then compare these policies from the origins of the national parks to the policies in place now regarding Indigenous people, such as the reparations that are trying to be made in these areas.
ContributorsSease, Emma Lynne (Author) / Richter, Jennifer (Thesis director) / Minteer, Ben (Committee member) / School of Life Sciences (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
Current culturing methods allow for human neural progenitor cells to be differentiated into neurons for use in diagnostic tools and disease modeling. An issue arises in the relatively low number of cells that can be successfully expanded and differentiated using these current methods, making the progress of research dependent on these cultures as a large number of cells are needed to conduct relevant assays. This project focuses on the expansion and differentiation of human neural progenitor cells cultured on microcarriers and within a rotating bioreactor system as a way to increase the total number of cells generated. Additionally, cryopreservation and the characteristics of these neurons post thaw is being investigated to create a way for long term storage, as well as, a method for standardizing cell lines between multiple experiments at different time points. The experiments covered in this study are aimed to compare the characteristics of differentiated human neurons, both demented and non-demented cell lines between pre-cryopreservation, freshly differentiated neurons and post-cryopreservation neurons. The assays conducted include immunofluorescence, calcium imaging, quantitative polymerase chain reaction, flow cytometry and ELISA data looking at Alzheimer’s disease traits. With the data collected within this study, the use of bioreactors, in addition to, cryopreservation of human neurons for long term storage can be better implemented into human neural progenitor cell research. Both of these aspects will increase the output of these cultures and potentially remove the bottleneck currently found within human neural disease modeling.
ContributorsHenson, Tanner Jay (Author) / Brafman, David (Thesis director) / Kodibagkar, Vikram (Committee member) / School of Life Sciences (Contributor) / Harrington Bioengineering Program (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
Homeless populations are often disproportionately impacted by several diseases due to factors such as overcrowding, unsanitary conditions, lack of access to healthcare and most importantly lack of education. The purpose of this project was to decrease a part of this health gap by spreading awareness of certain illnesses impacting Arizona’s homeless population and to increase the use plausible prevention methods. This was done through the creation of three simplified brochures that contained information regarding influenza, hepatitis, and schizophrenia. Two surveys were distributed to a local homeless population; the first survey was given prior to handing out the brochures and the second survey was given a week later after the participants had some time to read the information from the brochures. The data from the surveys supported the goal of the project by showing an increase in overall awareness of the diseases as well as an increase in behavioral changes that would lead to the increase of plausible prevention methods.
ContributorsBanuelos, Jason (Author) / Quaranta, Kimberly (Thesis director) / Szeli, Eva (Committee member) / School of Life Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05