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Description
Breast cancer is the leading cause of cancer-related deaths of women in the united states. Traditionally, Breast cancer is predominantly treated by a combination of surgery, chemotherapy, and radiation therapy. However, due to the significant negative side effects associated with these traditional treatments, there has been substantial efforts to develo

Breast cancer is the leading cause of cancer-related deaths of women in the united states. Traditionally, Breast cancer is predominantly treated by a combination of surgery, chemotherapy, and radiation therapy. However, due to the significant negative side effects associated with these traditional treatments, there has been substantial efforts to develop alternative therapies to treat cancer. One such alternative therapy is a peptide-based therapeutic cancer vaccine. Therapeutic cancer vaccines enhance an individual's immune response to a specific tumor. They are capable of doing this through artificial activation of tumor specific CTLs (Cytotoxic T Lymphocytes). However, in order to artificially activate tumor specific CTLs, a patient must be treated with immunogenic epitopes derived from their specific cancer type. We have identified that the tumor associated antigen, TPD52, is an ideal target for a therapeutic cancer vaccine. This designation was due to the overexpression of TPD52 in a variety of different cancer types. In order to start the development of a therapeutic cancer vaccine for TPD52-related cancers, we have devised a two-step strategy. First, we plan to create a list of potential TPD52 epitopes by using epitope binding and processing prediction tools. Second, we plan to attempt to experimentally identify MHC class I TPD52 epitopes in vitro. We identified 942 potential 9 and 10 amino acid epitopes for the HLAs A1, A2, A3, A11, A24, B07, B27, B35, B44. These epitopes were predicted by using a combination of 3 binding prediction tools and 2 processing prediction tools. From these 942 potential epitopes, we selected the top 50 epitopes ranked by a combination of binding and processing scores. Due to the promiscuity of some predicted epitopes for multiple HLAs, we ordered 38 synthetic epitopes from the list of the top 50 epitope. We also performed a frequency analysis of the TPD52 protein sequence and identified 3 high volume regions of high epitope production. After the epitope predictions were completed, we proceeded to attempt to experimentally detected presented TPD52 epitopes. First, we successful transduced parental K562 cells with TPD52. After transduction, we started the optimization process for the immunoprecipitation protocol. The optimization of the immunoprecipitation protocol proved to be more difficult than originally believed and was the main reason that we were unable to progress past the transduction of the parental cells. However, we believe that we have identified the issues and will be able to complete the experiment in the coming months.
ContributorsWilson, Eric Andrew (Author) / Anderson, Karen (Thesis director) / Borges, Chad (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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Description

Maricopa County is the home of the Phoenix metropolitan area, an expansive city with serious air quality concerns. To ameliorate air quality in the county, the Maricopa County Air Quality Department developed a website and mobile application called "Clean Air Make More" as a means of outreach and engagement. In

Maricopa County is the home of the Phoenix metropolitan area, an expansive city with serious air quality concerns. To ameliorate air quality in the county, the Maricopa County Air Quality Department developed a website and mobile application called "Clean Air Make More" as a means of outreach and engagement. In doing this, the county has found a way to engender a bilateral relationship between individuals and their government agency. This study analyzes the effectiveness of Clean Air Make More in establishing this relationship and engaging the community in efforts to improve air quality. It concludes that the design of the application effectively meets user needs, but marketing efforts should target populations disposed to taking action regarding air quality.

ContributorsLapoint, Maggie Lane (Author) / Johnston, Erik W., 1977- (Thesis director) / Hondula, David M. (Committee member) / Barrett, The Honors College (Contributor) / W. P. Carey School of Business (Contributor) / School of International Letters and Cultures (Contributor)
Created2015-05
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Description

Background:
Environmental heat exposure is a public health concern. The impacts of environmental heat on mortality and morbidity at the population scale are well documented, but little is known about specific exposures that individuals experience.

Objectives:
The first objective of this work was to catalyze discussion of the role of personal heat exposure

Background:
Environmental heat exposure is a public health concern. The impacts of environmental heat on mortality and morbidity at the population scale are well documented, but little is known about specific exposures that individuals experience.

Objectives:
The first objective of this work was to catalyze discussion of the role of personal heat exposure information in research and risk assessment. The second objective was to provide guidance regarding the operationalization of personal heat exposure research methods.

Discussion:
We define personal heat exposure as realized contact between a person and an indoor or outdoor environment that poses a risk of increases in body core temperature and/or perceived discomfort. Personal heat exposure can be measured directly with wearable monitors or estimated indirectly through the combination of time–activity and meteorological data sets. Complementary information to understand individual-scale drivers of behavior, susceptibility, and health and comfort outcomes can be collected from additional monitors, surveys, interviews, ethnographic approaches, and additional social and health data sets. Personal exposure research can help reveal the extent of exposure misclassification that occurs when individual exposure to heat is estimated using ambient temperature measured at fixed sites and can provide insights for epidemiological risk assessment concerning extreme heat.

Conclusions:
Personal heat exposure research provides more valid and precise insights into how often people encounter heat conditions and when, where, to whom, and why these encounters occur. Published literature on personal heat exposure is limited to date, but existing studies point to opportunities to inform public health practice regarding extreme heat, particularly where fine-scale precision is needed to reduce health consequences of heat exposure.

ContributorsKuras, Evan R. (Author) / Richardson, Molly B. (Author) / Calkins, Mirian M. (Author) / Ebi, Kristie L. (Author) / Gohlke, Julia M. (Author) / Hess, Jeremy J. (Author) / Hondula, David M. (Author) / Kintziger, Kristina W. (Author) / Jagger, Meredith A. (Author) / Middel, Ariane (Author) / Scott, Anna A. (Author) / Spector, June T. (Contributor) / Uejio, Christopher K. (Author) / Vanos, Jennifer K. (Author) / Zaitchik, Benjamin F. (Author)
Created2017-08
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Description
In June 2016, the Arizona Department of Health Services (ADHS) with researchers from Arizona State University (ASU) convened a one-day workshop of public health professionals and experts from Arizona’s county and state agencies to advance statewide preparedness for extreme weather events and climate change. The United States Centers for Disease

In June 2016, the Arizona Department of Health Services (ADHS) with researchers from Arizona State University (ASU) convened a one-day workshop of public health professionals and experts from Arizona’s county and state agencies to advance statewide preparedness for extreme weather events and climate change. The United States Centers for Disease Control and Prevention (CDC) sponsors the Climate-Ready Cities and States Initiative, which aims to help communities across the country prepare for and prevent projected disease burden associated with climate change. Arizona is one of 18 public health jurisdictions funded under this initiative. ADHS is deploying the CDC’s five-step Building Resilience Against Climate Effects (BRACE) framework to assist counties and local public health partners with becoming better prepared to face challenges associated with the impacts of climate-sensitive hazards. Workshop participants engaged in facilitated exercises designed to rigorously consider social vulnerability to hazards in Arizona and to prioritize intervention activities for extreme heat, wildfire, air pollution, and flooding.

This report summarizes the proceedings of the workshop focusing primarily on two sessions: the first related to social vulnerability mapping and the second related to the identification and prioritization of interventions necessary to address the impacts of climate-sensitive hazards.
ContributorsRoach, Matthew (Author) / Hondula, David M. (Author) / Putnam, Hana (Author) / Chhetri, Nalini (Author) / Chakalian, Paul (Author) / Watkins, Lance (Author) / Dufour, Brigette (Author)
Created2016-11-28