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- Creators: Ira A. Fulton Schools of Engineering
- Resource Type: Text
- Status: Published
Description
Fluoroquinolone antibiotics have been known to cause severe, multisystem adverse side effects, termed fluoroquinolone toxicity (FQT). This toxicity syndrome can present with adverse effects that vary from individual to individual, including effects on the musculoskeletal and nervous systems, among others. The mechanism behind FQT in mammals is not known, although various possibilities have been investigated. Among the hypothesized FQT mechanisms, those that could potentially explain multisystem toxicity include off-target mammalian topoisomerase interactions, increased production of reactive oxygen species, oxidative stress, and oxidative damage, as well as metal chelating properties of FQs. This review presents relevant information on fluoroquinolone antibiotics and FQT and explores the mechanisms that have been proposed. A fluoroquinolone-induced increase in reactive oxygen species and subsequent oxidative stress and damage presents the strongest evidence to explain this multisystem toxicity syndrome. Understanding the mechanism of FQT in mammals is important to aid in the prevention and treatment of this condition.
ContributorsHall, Brooke Ashlyn (Author) / Redding, Kevin (Thesis director) / Wideman, Jeremy (Committee member) / Borges, Chad (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description
Culture informs ideas about healthy and acceptable body types. Through globalization the U.S.-European body model has become increasingly significant in local contexts, influencing local body models. While Puerto Ricans have historically valued plump bodies - a biocultural legacy of a historically food scarce environment - this dissertation investigated shifts in these ideals across generations to a stronger preference for thinness. A sample of 23 intergenerational family triads of women, and one close male relative or friend per woman, were administered quantitative questionnaires. Ethnographic interviews were conducted with a sub-sample of women from 16 triads and 1 quintet. Questions about weight history and body sizes were used to address cultural changes in body models. Findings indicate the general trend for all generations has been a reduction in the spectrum of acceptable bodies to an almost singular idealized thin body. Female weight gain during puberty and influence of media produced varied responses across age groups. Overall, Puerto Ricans find it acceptable to gain weight with ageing, during a divorce, and postpartum. Thin bodies are associated with beauty and health, but healthy women that do not resemble the thin ideal, submit themselves to dangerous weight loss practices to achieve self and social acceptance. Further research and direct interventions need to be conducted to alter perceptions that conflate beauty with health in order to address the `normative discontent' women of all ages experience. Weight discrimination and concern with being overweight were evident in Puerto Rican everyday life, indicated by the role of media and acculturation in this study. Anti-fat attitudes were stronger for individuals that identified closely with United States culture. Exposure to drama and personal transformation television programs are associated with increased body image dissatisfaction, and increased exposure to variety shows and celebrity news shows is associated with increased anti-fat attitudes and body dissatisfaction. In sum, the positive valuation of fat in the Puerto Rican cultural body size model in the 1970s has shifted toward a negative valuation of fat and a preference for thin body size.
ContributorsRodriguez-Soto, Isa (Author) / Maupin, Jonathan (Thesis advisor) / Wutich, Amber (Committee member) / Walters-Pacheco, Kattia (Committee member) / Arizona State University (Publisher)
Created2013
Description
Despite the arid climate of Maricopa County, Arizona, vector-borne diseases have presented significant health challenges to the residents and public health professionals of Maricopa County in the past, and will continue to do so in the foreseeable future. Currently, West Nile virus is the only mosquitoes-transmitted disease actively, and natively, transmitted throughout the state of Arizona. In an effort to gain a more complete understanding of the transmission dynamics of West Nile virus this thesis examines human, vector, and environment interactions as they exist within Maricopa County. Through ethnographic and geographic information systems research methods this thesis identifies 1) the individual factors that influence residents' knowledge and behaviors regarding mosquitoes, 2) the individual and regional factors that influence residents' knowledge of mosquito ecology and the spatial distribution of local mosquito populations, and 3) the environmental, demographic, and socioeconomic factors that influence mosquito abundance within Maricopa County. By identifying the factors that influence human-vector and vector-environment interactions, the results of this thesis may influence current and future educational and mosquito control efforts throughout Maricopa County.
ContributorsKunzweiler, Colin (Author) / Boone, Christopher (Thesis advisor) / Wutich, Amber (Committee member) / Brewis-Slade, Alexandra (Committee member) / Arizona State University (Publisher)
Created2013
Description
Food deserts are the collection of deprived food environments and limit local residents from accessing healthy and affordable food. This dissertation research in San Lorenzo, Paraguay tests if the assumptions about food deserts in the Global North are also relevant to the Global South. In the Global South, the recent growth of supermarkets is transforming local food environments and may worsen residential food access, such as through emerging more food deserts globally. This dissertation research blends the tools, theories, and frameworks from clinical nutrition, public health, and anthropology to identify the form and impact of food deserts in the market city of San Lorenzo, Paraguay. The downtown food retail district and the neighborhood food environment in San Lorenzo were mapped to assess what stores and markets are used by residents. The food stores include a variety of formal (supermarkets) and informal (local corner stores and market vendors) market sources. Food stores were characterized using an adapted version of the Nutrition Environment Measures Survey for Stores (NEMS-S) to measure store food availability, affordability, and quality. A major goal in this dissertation was to identify how and why residents select a type of food store source over another using various ethnographic interviewing techniques. Residential store selection was linked to the NEMS-S measures to establish a connection between the objective quality of the local food environment, residential behaviors in the local food environment, and nutritional health status. Using a sample of 68 households in one neighborhood, modeling suggested the quality of local food environment does effect weight (measure as body mass index), especially for those who have lived longer in poorer food environments. More generally, I find that San Lorenzo is a city-wide food desert, suggesting that research needs to establish more nuanced categories of poor food environments to address how food environments emerge health concerns in the Global South.
ContributorsGartin, Meredith (Author) / Brewis Slade, Alexandra (Thesis advisor) / Boone, Christopher (Committee member) / Wutich, Amber (Committee member) / Arizona State University (Publisher)
Created2012
Description
Cancer claims hundreds of thousands of lives every year in US alone. Finding ways for early detection of cancer onset is crucial for better management and treatment of cancer. Thus, biomarkers especially protein biomarkers, being the functional units which reflect dynamic physiological changes, need to be discovered. Though important, there are only a few approved protein cancer biomarkers till date. To accelerate this process, fast, comprehensive and affordable assays are required which can be applied to large population studies. For this, these assays should be able to comprehensively characterize and explore the molecular diversity of nominally "single" proteins across populations. This information is usually unavailable with commonly used immunoassays such as ELISA (enzyme linked immunosorbent assay) which either ignore protein microheterogeneity, or are confounded by it. To this end, mass spectrometric immuno assays (MSIA) for three different human plasma proteins have been developed. These proteins viz. IGF-1, hemopexin and tetranectin have been found in reported literature to show correlations with many diseases along with several carcinomas. Developed assays were used to extract entire proteins from plasma samples and subsequently analyzed on mass spectrometric platforms. Matrix assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometric techniques where used due to their availability and suitability for the analysis. This resulted in visibility of different structural forms of these proteins showing their structural micro-heterogeneity which is invisible to commonly used immunoassays. These assays are fast, comprehensive and can be applied in large sample studies to analyze proteins for biomarker discovery.
ContributorsRai, Samita (Author) / Nelson, Randall (Thesis advisor) / Hayes, Mark (Thesis advisor) / Borges, Chad (Committee member) / Ros, Alexandra (Committee member) / Arizona State University (Publisher)
Created2012
Description
Bacteria play a vital role in the world ecosystem, more importantly human health and disease. The capability to differentiate and identify these microorganisms serves as an important research objective. In past years, separations-based approaches have served as a way to observe and identify bacteria based on their characteristics. Gradient insulator dielectrophoresis (g-iDEP) provides benefits in identifying serotypes of a single species with precise separation. Separation of Staphylococcus epidermidis in a single g-iDEP microchannel is conducted exploiting their electrophoretic and electrokinetic properties. The cells were captured and concentrated at gates with interacting forces within the microchannel to clearly distinguish between the two strains. These results provide support for g-iDEP serving as a separating method and, furthermore, future clinical applications.
ContributorsDavis, Paige Elizabeth (Author) / Hayes, Mark (Thesis director) / Borges, Chad (Committee member) / Jones, Paul (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor)
Created2015-05
Description
The study examines cross-cultural perceptions of wastewater reuse from 282 participants from four global sites representing varied levels of socio-economic and political development from the Global North and Global South: Spain, New Zealand, Fiji, and Guatemala. The data comes from the Global Ethnohydrology Survey conducted by the School of Human Evolution and Social Change during the summer of 2013. The Global Ethnohydrology Study is a transdisciplinary multi-year research initiative that examines the range of variation in local ecological knowledge of water issues, also known as "ethnohydrology." Participants were asked about their willingness, level of disgust, and concern with using treated wastewater for various daily activities. Additionally, they were asked to draw schematic representations of how wastewater should be treated to become drinkable again. Using visual content analysis, the drawings were coded for a variety of treatment levels and specific treatment processes. Conclusions about the perceived health implications from wastewater reuse that can stem from drinking treated wastewater were made. The relationship between humans and wastewater is one that has many direct social and health impacts on communities at large. In reaction to global limitations of freshwater, wastewater serves as a valuable resource to tap into. This research examines the cross-cultural public health concerns about treated wastewater in order to draw conclusions that can aid in strategic implementation of advocacy and public education about wastewater reuse.
ContributorsPatel, Sarah Shakir (Author) / Wutich, Amber (Thesis director) / Rice, Jacelyn (Committee member) / Barrett, The Honors College (Contributor) / School of Politics and Global Studies (Contributor) / School of Human Evolution and Social Change (Contributor)
Created2015-05
Description
Background: High risk types of human papillomavirus (HPV) are known to cause cancer, including cervical (99%) and oropharyngeal cancer (70%). HPV type 16 is the most common subtype. Three antigens that are critical for integration or tumor progression are E2, E6 and E7. In this study, we developed a systematic approach to identify naturally-processed HPV16-derived HLA class I epitopes for immunotherapy development. Methods: K562 cells, which lack HLA expression, were transduced with each HPV16 antigen using lentivirus and supertransfected with HLA-A2 by nucleofection. Stable cell lines expressing each antigen were selected for and maintained throughout the investigation. In order to establish a Gateway-compatible vector for robust transient gene expression, a Gateway recombination expression cloning cassette was inserted into the commercial Lonza pMAX GFP backbone, which has been experimentally shown to display high transfection expression efficiency. GFP was cloned into the vector and plain K562 cells were transfected with the plasmid by nucleofection. Results: Expression of K562-A2 was tested at various time points by flow cytometry and A2 expression was confirmed. Protein expression was shown for the transduced K562 E7 by Western blot analysis. High transfection efficiency of the pMAX_GFP_Dest vector (up to 97% GFP+ cells) was obtained 48 hours post transfection, comparable to the commercial GFP-plasmid. Conclusion: We have established a rapid system for target viral antigen co-expression with single HLA molecules for analysis of antigen presentation. Using HPV as a model system, our goal is to identify specific antigenic peptide sequences to develop immunotherapeutic treatments for HPV-associated cancers.
ContributorsVarda, Bianca Marie (Author) / Anderson, Karen (Thesis director) / Borges, Chad (Committee member) / Krishna, Sri (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Background: Latinos represent 40.8% of the population in Phoenix (U.S. Census Bureau Population Division, 2010). South Phoenix, also known as the South Mountain Village, defined in geographical terms as area zip codes 85040 and 85042; is a predominantly Latino community comprised of mixed citizenship status households. During the 2010 United States Census 60.3% of the population in South Phoenix identified as Latino, 25.75% of the total population was foreign born. Of the foreign born population, 88.95% were of Latin American origin (United States Census Bureau, 2007-2011 American Community Survey). Understanding how Latino immigrants perceive differences in health between their communities in country of origin and communities in the United States is largely unknown. Irrespective of political positions, understanding how Latino immigrants perceive personal health and the health of their communities is of interest to inform public policy and implement needed interventions in the
public health sphere.
Methods: Semi-structured interviews were collected from 55 adults from the South Phoenix community between November 2009 and September 2010. Interviews were digitally recorded with participant permission and transcribed. Of those collected, 48 transcribed interviews were analyzed using a codebook designed by the researcher. Percent agreement evaluated inter-rater reliability.Results: Latino immigrants in South Phoenix largely agree that health quality is heavily dependent on personal responsibility and not an intrinsic attribute of a given place. Emotional contentedness and distress, both factors of mental health, are impacted by cross-cultural differences between Latino and U.S. culture systems.
Conclusions: As people’s personal perceptions of differences in health are complex concepts influenced by personal backgrounds, culture, and beliefs, attempting to demark a side of the border as ‘healthier’ than the other using personal perceptions is overly simplified and misses central concepts. Instead, exploration of individual variables impacting health allowed this study to gain a more nuanced understanding in how people determine quality of both personal and environmental health. While Latino migrants in South Phoenix largely agree that health is based on personal responsibility and choices, many nonetheless experience higher levels of contentedness and emotional health in their country of origin.
public health sphere.
Methods: Semi-structured interviews were collected from 55 adults from the South Phoenix community between November 2009 and September 2010. Interviews were digitally recorded with participant permission and transcribed. Of those collected, 48 transcribed interviews were analyzed using a codebook designed by the researcher. Percent agreement evaluated inter-rater reliability.Results: Latino immigrants in South Phoenix largely agree that health quality is heavily dependent on personal responsibility and not an intrinsic attribute of a given place. Emotional contentedness and distress, both factors of mental health, are impacted by cross-cultural differences between Latino and U.S. culture systems.
Conclusions: As people’s personal perceptions of differences in health are complex concepts influenced by personal backgrounds, culture, and beliefs, attempting to demark a side of the border as ‘healthier’ than the other using personal perceptions is overly simplified and misses central concepts. Instead, exploration of individual variables impacting health allowed this study to gain a more nuanced understanding in how people determine quality of both personal and environmental health. While Latino migrants in South Phoenix largely agree that health is based on personal responsibility and choices, many nonetheless experience higher levels of contentedness and emotional health in their country of origin.
ContributorsGray, Laurel (Author) / Wutich, Amber (Thesis director) / Quiroga, S. Seline (Committee member) / Nelson, Margaret (Committee member) / Slade, B. Alexandra (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2013-05
Description
The Science of Water Art project is a collaborative work that brings together professionals, community members, college students and children to think about the role that water plays in each of our lives. Using a sample of 4th grade classrooms in Maricopa County, over 3000 drawings of children's perception of water today and in the future were collected. The 9-11 year olds were asked to draw pictures of 1) how they saw water being used in their neighborhood today (T1), and 2) how they imagined water would be used in their neighborhood 100 years from now (T2). The artwork was collected and coded for nine different themes, including: vegetation, scarcity, pollution, commercial sources of water, existing technology, technology innovation, recreational use, domestic use, and natural sources of water. Statistically significant differences were found between boys and girls for vegetation, technology and domestic use themes. This project allows for a look into how climate change and water insecurity is viewed by younger generations and gives a voice to children so that they may share their outlooks on this vital resource.
ContributorsVins, Holly Elizabeth (Author) / Wutich, Amber (Thesis director) / Newland, Judy (Committee member) / Beresford, Melissa (Committee member) / Barrett, The Honors College (Contributor) / School of International Letters and Cultures (Contributor) / School of Social Transformation (Contributor) / School of Human Evolution and Social Change (Contributor)
Created2013-05