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Description
Parkinson’s disease (PD) is a debilitating neurodegenerative disease characterized primarily by physical impairments such as tremors, poor balance, and bradykinesia; however, some individuals with PD will additionally experience numerous nonmotor symptoms such as dementia, depression, and sleep disturbances amongst various other life-altering ailments. Two of the key pathological hallmarks of PD include the death of melanated dopaminergic neurons in the nigrostriatal pathway and the accumulation of Lewy bodies, which are primarily composed of aggregates of the protein α-synuclein (α-syn). Interestingly, members of the chitinase protein family, namely chitinase-3-like protein-1 (L1), have heightened concentrations in a number of neurodegenerative diseases other than PD. To investigate the specific role L1 plays in PD etiology, we evaluated if astrocytic L1 expression was elevated in postmortem brain tissue of PD patients as well as in an α-syn overexpression rat model, and further tested if manipulating astrocytic-specific L1 expression correlated with neuroinflammation and nigral neuronal degeneration in the model. Preliminary histological analysis has shown increased levels of L1 expression in the α-syn model before neuronal loss occurs, and in human tissue, L1 was found to be significantly increased in the postmortem tissue of individuals with PD versus non-diseased controls. Investigations in identifying an astrocytic-specific virus capsid and manipulating L1 expression in the α-syn model are ongoing. This preliminary data thus far supports that increased astrocytic expression of L1 is associated with PD pathology.
ContributorsPettigrew, Tiffany (Author) / Manfredsson, Fredric (Thesis director) / Sandoval, Ivette (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2023-12
Description
Burnout due to work and school-related stress negatively impacts college student’s confidence, motivation, and mindset. Burnout can manifest as lower quality of a student’s education, performance, relationships, and overall well-being. Using the Maslach Burnout Inventory™ - General Survey for Students: MBI-GS (S), researchers assessed the effectiveness of a five-part workshop series titled “A Series on Self-Reflection, Self-Understanding, and Strengthening the Mind,” focused on improving burnout symptoms in students attending ASU’s Barrett, the Honors College, against a control group of non-workshop participating honors students. Data was collected through MBI-GS (S) evaluation surveys after each workshop attendance, via the daily honors newsletter, and through subject interviews.
The researchers conducted a qualitative study consistent with case studies and focus groups. Descriptive statistics of survey responses were quantified in Excel, representing varying burnout levels in honors students throughout the Spring 2023 school semester. Overall, the surveys from the workshop attendees and Honors Digest students yielded insignificant differences in burnout levels. The eight interviewee case studies emphasize the complex influences of culture and environment on burnout. While burnout levels between Honors Digest students and workshop attendees were insignificantly different, each interviewee student presented changed perspectives on their studies and themselves.
ContributorsBeyfuss, Natalie (Author) / Kappes, Janelle (Thesis director) / Schuster, Roseanne (Committee member) / Barrett, The Honors College (Contributor) / School of Human Evolution & Social Change (Contributor) / Edson College of Nursing and Health Innovation (Contributor) / School of Life Sciences (Contributor)
Created2023-12
Description
Current methods for quantifying microplastics via LC-MS/MS analysis have been adapted from environmental monitoring protocols and are often inadequate for sampling within complex matrices. This study explores the application of liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the detection of microplastics. The initial phase of this research utilized pork kidney samples to establish a baseline for background and efficacy of sample processing. These findings underscore the complexity of developing a sensitive and specific analytical technique for microplastics in tissues. The observed discrepancies in contamination and replicability between samples emphasize the need for continual method optimization.
ContributorsBabbrah, Ayesha (Author) / Halden, Rolf (Thesis director) / Newell, Melanie (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2023-12
Description
Originally conceived as a way to scaffold molecules of interest into three-dimensional (3D) crystalline lattices for X ray crystallography, the field of deoxyribonucleic acid (DNA) nanotechnology has dramatically evolved since its inception. The unique properties of DNA nanostructures have promoted their use not only for X ray crystallography, but for a suite of biomedical applications as well. The work presented in this dissertation focuses on both of these exciting applications in the field: 1) Nucleic acid nanostructures as multifunctional drug and vaccine delivery platforms, and 2) 3D DNA crystals for structure elucidation of scaffolded guest molecules.Chapter 1 illustrates how a wide variety of DNA nanostructures have been developed for the delivery of drugs and vaccine components. However, their applications are limited under physiological conditions due to their lack of stability in low salt environments, susceptibility to enzymatic degradation, and tendency for endosomal entrapment. To address these issues, Chapter 2 describes a PEGylated peptide coating molecule was designed to electrostatically adhere to and protect DNA origami nanostructures and to facilitate their cytosolic delivery by peptide-mediated endosomal escape. The development of this molecule will aid in the use of nucleic acid nanostructures for biomedical purposes, such as the delivery of messenger ribonucleic acid (mRNA) vaccine constructs. To this end, Chapter 3 discusses the fabrication of a structured mRNA nanoparticle for more cost-efficient mRNA vaccine manufacture and proposes a multi-epitope mRNA nanostructure vaccine design for targeting human papillomavirus (HPV) type 16-induced head and neck cancers.
DNA nanotechnology was originally envisioned to serve as three-dimensional scaffolds capable of positioning proteins in a rigid array for their structure elucidation by X ray crystallography. Accordingly, Chapter 4 explores design parameters, such as sequence and Holliday junction isomeric forms, for efficient crystallization of 3D DNA lattices. Furthermore, previously published DNA crystal motifs are used to site-specifically position and structurally evaluate minor groove binding molecules with defined occupancies. The results of this study provide significant advancement towards the ultimate goal of the field.
ContributorsHenry, Skylar J.W. (Author) / Stephanopoulos, Nicholas (Thesis advisor) / Anderson, Karen (Thesis advisor) / Blattman, Joseph (Committee member) / Yan, Hao (Committee member) / Arizona State University (Publisher)
Created2023
Description
Alzheimer's disease (AD) is characterized by the progressive loss of cognitive function and memory, and is the most common cause of dementia. Alzheimer's disease targets the brain and is associated with the accumulation of abnormal protein aggregates that disrupt neural network communication and lead to various facets of cognitive decline. Current problems in AD research originates from the inability to identify individuals that are at risk. With the limitations of patients already displaying signs of neurodegeneration, strategies for prevention are not effective. Through developing a comprehensive cognitive test that tests a range of mental abilities, data can be collected, and a longitudinal study can be proposed to track cognitive decline in a multifaceted approach over a period of time. Previous literature indicates that functional decline is present years before the manifestation of symptoms in Alzheimer’s disease. Identifying pre-diagnostic cognitive and functional changes could lead to an improved and wider selection for preventive treatment trials. By designing a comprehensive cognitive experiment, an initial data set was obtained, and trends related to time and answer response were analyzed. The scope, applications and limitations within this experimental design is discussed, and future directions are proposed.
ContributorsDale, Anna (Author) / Hartwell, Leland (Thesis director) / Huseby, Carol (Committee member) / Barrett, The Honors College (Contributor) / School of Music, Dance and Theatre (Contributor) / School of Life Sciences (Contributor)
Created2023-12
ContributorsOsha, Peyton (Author) / Alperin, Kate (Co-author) / James, Darith (Thesis director) / Mun, Chung Jung (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / School of Human Evolution & Social Change (Contributor)
Created2023-12
ContributorsOsha, Peyton (Author) / Alperin, Kate (Co-author) / James, Darith (Thesis director) / Mun, Chung Jung (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / School of Human Evolution & Social Change (Contributor)
Created2023-12
Description
The objective of this meta-analysis is to holistically evaluate the existing body of literature on the anti-neoplastic potential of snake and bee venom. In recent years, venom-based therapeutics have emerged as a promising solution for combating cancer, generating a notable rise in publications on the topic. Consequently, this comprehensive study aims to assess the current state of research and identify trends that may guide future investigations. Following the guidelines established by PRISMA, a total sample of 26 research papers were extracted from the electronic databases, PubMed and Scopus. These papers were categorized based on their publication dates, and research questions were formulated regarding three main topics: venom type, cancer-targeting mechanism, and cancer type. Statistical analysis of the research questions was performed using 2x2 contingency tables for a chi-square test. The results of the analysis reveal a statistically significant increase in publications focused on cell death mechanisms and breast cancer in recent years. This increase in publications reflects a growing interest in the potential for venom to induce apoptosis in cancer cells and target the unique biological properties of breast cancer. Overall, this meta-analysis offers insight into the evolving sphere of venom-based cancer research, providing a glimpse into the potential trajectory of this field.
ContributorsHolder, Marina (Author) / Amdam, Gro (Thesis director) / Mana, Miyeko (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / Economics Program in CLAS (Contributor)
Created2023-12
Description
A review of the experience of running a fully-remote, nationwide clinical trial centered around intermittent fasting and its effects on cognitive health in a midlife population that is living with stress, obesity, and a memory that is not as good as it used to be. As the two project managers, we had a myriad of responsibilities, which came with many challenges and successes. The preliminary results have been analyzed and the study is now in the process of manuscript development.
ContributorsOsha, Peyton (Author) / Alperin, Kate (Co-author) / James, Darith (Thesis director) / Mun, Chung Jung (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / School of Human Evolution & Social Change (Contributor)
Created2023-12
Description
Climate change is a well-known global threat to societal systems; however, its effects on
the health of individuals are often less evident. Physicians who aim to properly treat patients
holistically must be educated on the various forms of illness and disease projected to be
exacerbated by climate change. Without this necessary climate education, physicians run the risk of being unable to fulfill the most sacred charge of the Hippocratic oath: Do No Harm. To
ensure that physicians moving forward are prepared to face this new global health threat, the
prevalence of climate change in current medical school curricula must be examined. Content
analysis of publicly available medical school curricula in the Southwest U.S. was done using
ChatGPT to track the frequency of climate health search terms. Medical school curricula
analyzed included mandatory degree programs as well as optional dual degree programs or
pathways for medical student education. Researchers found that medical schools within the
Southwest region of the United States are not sufficiently preparing students to mitigate the
regional effects of climate change on the health of patients. Mandatory medical degree
curriculum does not sufficiently educate on climate health issues, nor is it present in Utah, New
Mexico, or Colorado. Optional degrees and pathways are available to medical students to enroll
in and may be sufficient to educate a medical student, but are not enticing enough to sufficiently
educate all medical students. Some medical schools have recently conducted revisals of their
mandatory curriculum and still show a lack of education available about climate health issues.
The lack of educational resources for future providers could lead to detrimental health outcomes
for patients, and medical schools in development should take the lead in educating their students about climate health issues.
ContributorsGutierrez, Sean (Author) / Jamieson, Ann (Co-author) / Vanos, Jennifer (Thesis director) / Lobo, Jose (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / Historical, Philosophical & Religious Studies, Sch (Contributor)
Created2023-12