Matching Items (548)
Description
Filtration for microfluidic sample-collection devices is desirable for sample selection, concentration, preprocessing, and downstream manipulation, but microfabricating the required sub-micrometer filtration structure is an elaborate process. This thesis presents a simple method to fabricate polydimethylsiloxane (PDMS) devices with an integrated membrane filter that will sample, lyse, and extract the DNA

Filtration for microfluidic sample-collection devices is desirable for sample selection, concentration, preprocessing, and downstream manipulation, but microfabricating the required sub-micrometer filtration structure is an elaborate process. This thesis presents a simple method to fabricate polydimethylsiloxane (PDMS) devices with an integrated membrane filter that will sample, lyse, and extract the DNA from microorganisms in aqueous environments. An off-the-shelf membrane filter disc was embedded in a PDMS layer and sequentially bound with other PDMS channel layers. No leakage was observed during filtration. This device was validated by concentrating a large amount of cyanobacterium Synechocystis in simulated sample water with consistent performance across devices. After accumulating sufficient biomass on the filter, a sequential electrochemical lysing process was performed by applying 5VDC across the filter. This device was further evaluated by delivering several samples of differing concentrations of cyanobacterium Synechocystis then quantifying the DNA using real-time PCR. Lastly, an environmental sample was run through the device and the amount of photosynthetic microorganisms present in the water was determined. The major breakthroughs in this design are low energy demand, cheap materials, simple design, straightforward fabrication, and robust performance, together enabling wide-utility of similar chip-based devices for field-deployable operations in environmental micro-biotechnology.
ContributorsLecluse, Aurelie (Author) / Meldrum, Deirdre (Thesis advisor) / Chao, Joseph (Thesis advisor) / Westerhoff, Paul (Committee member) / Arizona State University (Publisher)
Created2011
148113-Thumbnail Image.png
Description

In the past decade, a significant shift has emerged around immigration policy, as advocates and policymakers have made various efforts to pass state and local policies related to immigrant integration or restrictions. This thesis offers original insights into current dynamics in immigration federalism through interviews with lawmakers and community activists

In the past decade, a significant shift has emerged around immigration policy, as advocates and policymakers have made various efforts to pass state and local policies related to immigrant integration or restrictions. This thesis offers original insights into current dynamics in immigration federalism through interviews with lawmakers and community activists in Arizona, a leading state when it comes to restricting the lives of undocumented immigrants. Advancing a new framework that connects the lived experience of officials and activists to partisanship, policy, key events, demographics, and racializing events, this thesis bridges isolated bodies of scholarship on immigration and seeks to demonstrate how every person (not just immigrant) are part of America’s current challenges to become a more inclusive nation of immigrants.

ContributorsNeville, Christopher Francis (Author) / Colbern, Allan (Thesis director) / Martinez-Orosco, Rafael (Committee member) / School of Social and Behavioral Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
148117-Thumbnail Image.png
Description

Something Like Human explores corporate social responsibility through a triple lens, providing a content analysis using previous literature and history as the standards for evaluation. Section I reviews the history of corporate social responsibility and how it is currently understood and employed today. Section II turns its focus to a

Something Like Human explores corporate social responsibility through a triple lens, providing a content analysis using previous literature and history as the standards for evaluation. Section I reviews the history of corporate social responsibility and how it is currently understood and employed today. Section II turns its focus to a specific socially conscious corporation, Lush Cosmetics, examining its practices considering the concepts provided in Section I and performing a close analysis of its promotional materials. Section III consists of a mock marketing campaign designed for Lush in light of their social commitments. By the end of this thesis, the goal for the reader is to ask: Can major corporations be something like human?

ContributorsDalgleish, Alayna Rose (Author) / Gruber, Diane (Thesis director) / Thornton, Leslie-Jean (Committee member) / School of Social and Behavioral Sciences (Contributor, Contributor) / Walter Cronkite School of Journalism and Mass Comm (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
147854-Thumbnail Image.png
Description

Veterans are approximately 30% more likely than non-veterans to suffer from severe hearing impairment. Tinnitus, or ringing in the ears, which is increasingly common among military service men and women, has been linked to significant cognitive and psychological impairment and can be worsened by the same sounds that trigger post-traumatic

Veterans are approximately 30% more likely than non-veterans to suffer from severe hearing impairment. Tinnitus, or ringing in the ears, which is increasingly common among military service men and women, has been linked to significant cognitive and psychological impairment and can be worsened by the same sounds that trigger post-traumatic stress disorder (PTSD). In fact, tinnitus and PTSD often present as comorbidities, and recent studies suggest these two disorders may share a common neurological pathway. Additional studies are required to better understand the connection between hearing loss and impaired cognitive function such as that observed in with PTSD. Here, we use the fruit fly, Drosophila melanogaster, to explore the relationship between hearing loss and cognitive function. Negative geotaxis climbing assays and courtship behavior analysis were used to examine neurobehavioral changes induced by prolonged, intense auditory stimulation. Preliminary results suggest that exposure to loud noise for an extended period of time significantly affected Drosophila behavior, with males being more sensitive than females. Based on our results, there appears to be a potential connection between noise exposure and behavior, further suggesting that Drosophila could be an effective model to study the link between hearing loss and PTSD.

ContributorsMichael, Allison Faye (Author) / Hackney-Price, Jennifer (Thesis director) / Sellner, Erin (Committee member) / School of Social and Behavioral Sciences (Contributor) / School of Mathematical and Natural Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
147864-Thumbnail Image.png
Description

Socioeconomic differences have driven society and laid the foundation for the types of opportunities and resources one is eligible to receive. Higher socioeconomic status provides individuals the chance of obtaining an overall better education, occupation, and income. We see this with particular clarity when we examine healthcare. The World Health

Socioeconomic differences have driven society and laid the foundation for the types of opportunities and resources one is eligible to receive. Higher socioeconomic status provides individuals the chance of obtaining an overall better education, occupation, and income. We see this with particular clarity when we examine healthcare. The World Health Organization has regarded healthcare as a fundamental human right, except socioeconomically disadvantaged individuals not only do not have equal access to healthcare, but they also often receive a lower quality of care. These socioeconomic differences are often paired with racial differences, resulting in one group, or set of groups, having social advantage over the others. Although this problem has been discussed throughout the past century, it has not been properly addressed materially and practically. Unequal access to quality healthcare is especially highlighted throughout the COVID-19 pandemic, where there has been evidence that minorities, in particular Black communities, have received inadequate care. Quality healthcare has become unaffordable and a luxury that only certain groups get the privilege of receiving. Not only that, but the ongoing inequalities in the healthcare system have gone so far that they have instilled hostility and mistrust towards the healthcare system.

ContributorsMartinez Castro, Karen (Author) / Sturgess, Jessica (Thesis director) / Sellner, Erin (Committee member) / School of Social and Behavioral Sciences (Contributor) / School of Mathematical and Natural Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
147984-Thumbnail Image.png
Description

In 2020, the world was swept by a global pandemic. It disrupted the lives of millions; many lost their jobs, students were forced to leave schools, and children were left with little to do while quarantined at their houses. Although the media outlets covered very little of how children were

In 2020, the world was swept by a global pandemic. It disrupted the lives of millions; many lost their jobs, students were forced to leave schools, and children were left with little to do while quarantined at their houses. Although the media outlets covered very little of how children were being affected by COVID-19, it was obvious that their group was not immune to the issues the world was facing. Being stuck at home with little to do took a mental and physical toll on many kids. That is when EVOLVE Academy became an idea; our team wanted to create a fully online platform for children to help them practice and evolve their athletics skills, or simply spend part of their day performing a physical and health activity. Our team designed a solution that would benefit children, as well as parents that were struggling to find engaging activities for their kids while out of school. We quickly encountered issues that made it difficult for us to reach our target audience and make them believe and trust our platform. However, we persisted and tried to solve and answer the questions and problems that came along the way. Sadly, the same pandemic that opened the widow for EVOLVE Academy to exist, is now the reason people are walking away from it. Children want real interaction. They want to connect with other kids through more than just a screen. Although the priority of parents remains the safety and security of their kids, parents are also searching and opting for more “human” interactions, leaving EVOLVE Academy with little room to grow and succeed.

ContributorsHernandez, Melany (Co-author) / Parmenter, Taylor (Co-author) / Byrne, Jared (Thesis director) / Kunowski, Jeffrey (Committee member) / Lee, Christopher (Committee member) / Thunderbird School of Global Management (Contributor, Contributor) / School of Social and Behavioral Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description

Evidence of a widening political divide between the Democrat and Republican parties creates concern over the possible ramifications of this phenomenon. One method of assessing dissonance between political parties is through a medium that is shaped by personal beliefs: online dating. Previous research finds that political choice homophily, or a

Evidence of a widening political divide between the Democrat and Republican parties creates concern over the possible ramifications of this phenomenon. One method of assessing dissonance between political parties is through a medium that is shaped by personal beliefs: online dating. Previous research finds that political choice homophily, or a preference for those who are politically similar, is relevant in online dating settings. Researchers find that individuals are significantly more likely to be attracted to or pursue others who have similar political views to themselves. There is, however, a gap in knowledge regarding online dating that occurs separately from conventional online dating sites. Mobile dating apps, applications that are highly popular amongst young adults, have not been thoroughly explored with political choice homophily in mind. The current study aims to test the correlation between dating preference and political beliefs, using the mobile dating app Bumble as a template for a mock (fake) dating app scenario. In the dating app survey that was distributed to 132 ASU students, participants completed a simulated “matching” section where they matched with a list of 15 fabricated Bumble profiles. The fake Bumble profiles randomly contained politically charged or politically neutral statements in the basic info section. Political affiliation of the participant was measured using a sliding scale that quantifies the unidimensional conservative-liberal spectrum on a 0-100 numerical scale. Findings of the survey show that there was no significant difference between participant preference towards politically charged vs. politically neutral mock profiles.

ContributorsGilmore, Ethan James (Author) / Lewis, Stephen (Thesis director) / Halavais, Alexander (Committee member) / School of Social and Behavioral Sciences (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description
Single cell phenotypic heterogeneity studies reveal more information about the pathogenesis process than conventional bulk methods. Furthermore, investigation of the individual cellular response mechanism during rapid environmental changes can only be achieved at single cell level. By enabling the study of cellular morphology, a single cell three-dimensional (3D) imaging system

Single cell phenotypic heterogeneity studies reveal more information about the pathogenesis process than conventional bulk methods. Furthermore, investigation of the individual cellular response mechanism during rapid environmental changes can only be achieved at single cell level. By enabling the study of cellular morphology, a single cell three-dimensional (3D) imaging system can be used to diagnose fatal diseases, such as cancer, at an early stage. One proven method, CellCT, accomplishes 3D imaging by rotating a single cell around a fixed axis. However, some existing cell rotating mechanisms require either intricate microfabrication, and some fail to provide a suitable environment for living cells. This thesis develops a microvorterx chamber that allows living cells to be rotated by hydrodynamic alone while facilitating imaging access. In this thesis work, 1) the new chamber design was developed through numerical simulation. Simulations revealed that in order to form a microvortex in the side chamber, the ratio of the chamber opening to the channel width must be smaller than one. After comparing different chamber designs, the trapezoidal side chamber was selected because it demonstrated controllable circulation and met the imaging requirements. Microvortex properties were not sensitive to the chambers with interface angles ranging from 0.32 to 0.64. A similar trend was observed when chamber heights were larger than chamber opening. 2) Micro-particle image velocimetry was used to characterize microvortices and validate simulation results. Agreement between experimentation and simulation confirmed that numerical simulation was an effective method for chamber design. 3) Finally, cell rotation experiments were performed in the trapezoidal side chamber. The experimental results demonstrated cell rotational rates ranging from 12 to 29 rpm for regular cells. With a volumetric flow rate of 0.5 µL/s, an irregular cell rotated at a mean rate of 97 ± 3 rpm. Rotational rates can be changed by altering inlet flow rates.
ContributorsZhang, Wenjie (Author) / Frakes, David (Thesis advisor) / Meldrum, Deirdre (Thesis advisor) / Chao, Shih-hui (Committee member) / Wang, Xiao (Committee member) / Arizona State University (Publisher)
Created2011
Description

This thesis will assess the relationship between race and perceptions of incarceration through responses gathered from interviews administered to men incarcerated at the Arizona State Prison Complex in Florence, Arizona. The interviews were conducted by incarcerated men through a Participatory Action Research (PAR) project to enhance the prison environment. Critically,

This thesis will assess the relationship between race and perceptions of incarceration through responses gathered from interviews administered to men incarcerated at the Arizona State Prison Complex in Florence, Arizona. The interviews were conducted by incarcerated men through a Participatory Action Research (PAR) project to enhance the prison environment. Critically, men who were interviewed answered the question “What would you do if you were the Director of the Department of Corrections?” The purpose of this thesis is twofold. First, what are the major themes provided to this question? Second, did these themes differ depending on the race or ethnicity of the respondent? The results from this survey can provide a more informed future for corrections that acknowledges the unique criminal justice system experiences held by members of different racial and ethnic groups.

ContributorsQuintero, Karen Rotna (Author) / Wright, Kevin (Thesis director) / Young, Jacob (Committee member) / School of Social and Behavioral Sciences (Contributor) / School of International Letters and Cultures (Contributor) / College of Health Solutions (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description
Laboratory automation systems have seen a lot of technological advances in recent times. As a result, the software that is written for them are becoming increasingly sophisticated. Existing software architectures and standards are targeted to a wider domain of software development and need to be customized in order to use

Laboratory automation systems have seen a lot of technological advances in recent times. As a result, the software that is written for them are becoming increasingly sophisticated. Existing software architectures and standards are targeted to a wider domain of software development and need to be customized in order to use them for developing software for laboratory automation systems. This thesis proposes an architecture that is based on existing software architectural paradigms and is specifically tailored to developing software for a laboratory automation system. The architecture is based on fairly autonomous software components that can be distributed across multiple computers. The components in the architecture make use of asynchronous communication methodologies that are facilitated by passing messages between one another. The architecture can be used to develop software that is distributed, responsive and thread-safe. The thesis also proposes a framework that has been developed to implement the ideas proposed by the architecture. The framework is used to develop software that is scalable, distributed, responsive and thread-safe. The framework currently has components to control very commonly used laboratory automation devices such as mechanical stages, cameras, and also to do common laboratory automation functionalities such as imaging.
ContributorsKuppuswamy, Venkataramanan (Author) / Meldrum, Deirdre (Thesis advisor) / Collofello, James (Thesis advisor) / Sarjoughian, Hessam S. (Committee member) / Johnson, Roger (Committee member) / Arizona State University (Publisher)
Created2012