Matching Items (20)
Description
Although conflict is a normative part of parent–adolescent relationships, conflicts that are long or highly negative are likely to be detrimental to these relationships and to youths’ development. In the present article, sequential analyses of data from 138 parent–adolescent dyads (adolescents’ mean age was 13.44, SD = 1.16; 52 % girls, 79 % non-Hispanic White) were used to define conflicts as reciprocal exchanges of negative emotion observed while parents and adolescents were discussing “hot,” conflictual issues. Dynamic components of these exchanges, including who started the conflicts, who ended them, and how long they lasted, were identified. Mediation analyses revealed that a high proportion of conflicts ended by adolescents was associated with longer conflicts, which in turn predicted perceptions of the “hot” issue as unresolved and adolescent behavior problems. The findings illustrate advantages of using sequential analysis to identify patterns of interactions and, with some certainty, obtain an estimate of the contingent relationship between a pattern of behavior and child and parental outcomes. These interaction patterns are discussed in terms of the roles that parents and children play when in conflict with each other, and the processes through which these roles affect conflict resolution and adolescents’ behavior problems.
ContributorsMoed, Anat (Author) / Gershoff, Elizabeth T. (Author) / Eisenberg, Nancy (Author) / Hofer, Claire (Author) / Losoya, Sandra (Author) / Spinrad, Tracy (Author) / Liew, Jeffrey (Author) / College of Liberal Arts and Sciences (Contributor) / Department of Psychology (Contributor) / Sanford School of Social and Family Dynamics (Contributor)
Created2015-08-01
Description
X-ray free-electron lasers provide novel opportunities to conduct single particle analysis on nanoscale particles. Coherent diffractive imaging experiments were performed at the Linac Coherent Light Source (LCLS), SLAC National Laboratory, exposing single inorganic core-shell nanoparticles to femtosecond hard-X-ray pulses. Each facetted nanoparticle consisted of a crystalline gold core and a differently shaped palladium shell. Scattered intensities were observed up to about 7 nm resolution. Analysis of the scattering patterns revealed the size distribution of the samples, which is consistent with that obtained from direct real-space imaging by electron microscopy. Scattering patterns resulting from single particles were selected and compiled into a dataset which can be valuable for algorithm developments in single particle scattering research.
ContributorsLi, Xuanxuan (Author) / Chiu, Chun-Ya (Author) / Wang, Hsiang-Ju (Author) / Kassemeyer, Stephan (Author) / Botha, Sabine (Author) / Shoeman, Robert L. (Author) / Lawrence, Robert (Author) / Kupitz, Christopher (Author) / Kirian, Richard (Author) / James, Daniel (Author) / Wang, Dingjie (Author) / Nelson, Garrett (Author) / Messerschmidt, Marc (Author) / Boutet, Sebastien (Author) / Williams, Garth J. (Author) / Hartman, Elisabeth (Author) / Jafarpour, Aliakbar (Author) / Foucar, Lutz M. (Author) / Barty, Anton (Author) / Chapman, Henry (Author) / Liang, Mengning (Author) / Menzel, Andreas (Author) / Wang, Fenglin (Author) / Basu, Shibom (Author) / Fromme, Raimund (Author) / Doak, R. Bruce (Author) / Fromme, Petra (Author) / Weierstall, Uwe (Author) / Huang, Michael H. (Author) / Spence, John (Author) / Schlichting, Ilme (Author) / Hogue, Brenda (Author) / Liu, Haiguang (Author) / ASU Biodesign Center Immunotherapy, Vaccines and Virotherapy (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / College of Liberal Arts and Sciences (Contributor) / School of Molecular Sciences (Contributor) / Department of Physics (Contributor) / School of Life Sciences (Contributor)
Created2017-04-11
Description
Serial femtosecond X-ray crystallography (SFX) using an X-ray free electron laser (XFEL) is a recent advancement in structural biology for solving crystal structures of challenging membrane proteins, including G-protein coupled receptors (GPCRs), which often only produce microcrystals. An XFEL delivers highly intense X-ray pulses of femtosecond duration short enough to enable the collection of single diffraction images before significant radiation damage to crystals sets in. Here we report the deposition of the XFEL data and provide further details on crystallization, XFEL data collection and analysis, structure determination, and the validation of the structural model. The rhodopsin-arrestin crystal structure solved with SFX represents the first near-atomic resolution structure of a GPCR-arrestin complex, provides structural insights into understanding of arrestin-mediated GPCR signaling, and demonstrates the great potential of this SFX-XFEL technology for accelerating crystal structure determination of challenging proteins and protein complexes.
ContributorsZhou, X. Edward (Author) / Gao, Xiang (Author) / Barty, Anton (Author) / Kang, Yanyong (Author) / He, Yuanzheng (Author) / Liu, Wei (Author) / Ishchenko, Andrii (Author) / White, Thomas A. (Author) / Yefanov, Oleksandr (Author) / Han, Gye Won (Author) / Xu, Qingping (Author) / de Waal, Parker W. (Author) / Suino-Powell, Kelly M. (Author) / Boutet, Sebastien (Author) / Williams, Garth J. (Author) / Wang, Meitian (Author) / Li, Dianfan (Author) / Caffrey, Martin (Author) / Chapman, Henry N. (Author) / Spence, John (Author) / Fromme, Petra (Author) / Weierstall, Uwe (Author) / Stevens, Raymond C. (Author) / Cherezov, Vadim (Author) / Melcher, Karsten (Author) / Xu, H. Eric (Author) / College of Liberal Arts and Sciences (Contributor) / School of Molecular Sciences (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / Department of Physics (Contributor)
Created2016-04-12
Description
Diacylglycerol kinase catalyses the ATP-dependent conversion of diacylglycerol to phosphatidic acid in the plasma membrane of Escherichia coli. The small size of this integral membrane trimer, which has 121 residues per subunit, means that available protein must be used economically to craft three catalytic and substrate-binding sites centred about the membrane/cytosol interface. How nature has accomplished this extraordinary feat is revealed here in a crystal structure of the kinase captured as a ternary complex with bound lipid substrate and an ATP analogue. Residues, identified as essential for activity by mutagenesis, decorate the active site and are rationalized by the ternary structure. The γ-phosphate of the ATP analogue is positioned for direct transfer to the primary hydroxyl of the lipid whose acyl chain is in the membrane. A catalytic mechanism for this unique enzyme is proposed. The active site architecture shows clear evidence of having arisen by convergent evolution.
ContributorsLi, Dianfan (Author) / Stansfeld, Phillip J. (Author) / Sansom, Mark S. P. (Author) / Keogh, Aaron (Author) / Vogeley, Lutz (Author) / Howe, Nicole (Author) / Lyons, Joseph A. (Author) / Aragao, David (Author) / Fromme, Petra (Author) / Fromme, Raimund (Author) / Basu, Shibom (Author) / Grotjohann, Ingo (Author) / Kupitz, Christopher (Author) / Rendek, Kimberley (Author) / Weierstall, Uwe (Author) / Zatsepin, Nadia (Author) / Cherezov, Vadim (Author) / Liu, Wei (Author) / Bandaru, Sateesh (Author) / English, Niall J. (Author) / Gati, Cornelius (Author) / Barty, Anton (Author) / Yefanov, Oleksandr (Author) / Chapman, Henry N. (Author) / Diederichs, Kay (Author) / Messerschmidt, Marc (Author) / Boutet, Sebastien (Author) / Williams, Garth J. (Author) / Seibert, M. Marvin (Author) / Caffrey, Martin (Author) / College of Liberal Arts and Sciences (Contributor) / School of Molecular Sciences (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / Department of Physics (Contributor)
Created2015-12-17
Description
The purpose of this study was to examine whether dispositional sadness predicted children's prosocial behavior and if sympathy mediated this relation. Constructs were measured when children (n = 256 at time 1) were 18, 30, and 42 months old. Mothers and non-parental caregivers rated children's sadness; mothers, caregivers, and fathers rated children's prosocial behavior; sympathy (concern and hypothesis testing) and prosocial behavior (indirect and direct, as well as verbal at older ages) were assessed with a task in which the experimenter feigned injury. In a panel path analysis, 30-month dispositional sadness predicted marginally higher 42-month sympathy; in addition, 30-month sympathy predicted 42-month sadness. Moreover, when controlling for prior levels of prosocial behavior, 30-month sympathy significantly predicted reported and observed prosocial behavior at 42 months. Sympathy did not mediate the relation between sadness and prosocial behavior (either reported or observed).
ContributorsEdwards, Alison (Author) / Eisenberg, Nancy (Author) / Spinrad, Tracy (Author) / Reiser, Mark (Author) / Wilkens, Natalie (Author) / Liew, Jeffrey (Author) / College of Liberal Arts and Sciences (Contributor) / Department of Psychology (Contributor) / Sanford School of Social and Family Dynamics (Contributor) / School of Mathematical and Statistical Sciences (Contributor)
Created2015-01-01
Description
In recent years, educational policy in the United States has focused extensively on the importance of providing American students with quality training in science, technology, engineering, and mathematics fields (STEM). It is generally agreed upon that STEM fields will provide a large number of the future economy's jobs, and that America needs more students to be adept in STEM skills in order for the country to remain a global economic leader. Discourse has also centered around the gender disparity in these fields; even though women have surpassed men in overall college degree attainment over the last couple decades, there are far fewer women than men in many STEM majors and occupations. STEM and gender inequality has been studied extensively, and the U.S. Department of Education is continuing its research efforts to understand the factors that lead women to choose STEM field with the aim of enlarging the pool of students enter STEM fields. In 2009, the department began a longitudinal study that followed 9th graders into their college years. This data set (the HLS:09) was used in order to assess gender disparities in STEM with a recent, nationally representative sample. Logistic Regression analysis was used in order to identity social variables that interact with gender to predict whether or not a student would choose a STEM major upon entering college. The results of the analysis are considered through a critical lens and discussion of how the social hierarchy of fields of work through occupational income and cultural prestige (i.e. when presidential administrations promote STEM education due to job growth in STEM industries) reproduces inequality through constraining students' choices in college majors and work fields whether or not gender equality is realized.
ContributorsGorry, Lindsey Elaine (Author) / LePore, Paul (Thesis director) / Wilkens, Natalie (Committee member) / Department of Psychology (Contributor) / Sanford School of Social and Family Dynamics (Contributor) / School of Social Transformation (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
I investigated if race/ethnicity was associated with self- and peer-reported victimization and aggression in a sample of 5th through 8th graders (N = 383, 51% males) from two schools in which Hispanic/Latino students were the ethnic-racial majority. Self-reported victimization did not differ between races. In contrast, White students often had higher peer-reported victimization relative to Hispanic and Multi-racial students. Few significant associations were found for aggression. There was some, albeit inconsistent, support for the idea that power imbalance based on race/ethnicity is shifted by numbers. In the future, researchers should conduct studies aimed verifying this notion and that are tailored toward answering questions of mechanism.
ContributorsMitiku, Helen (Author) / Wilkens, Natalie (Thesis director) / Lindstrom Johnson, Sarah (Committee member) / White, Rebecca (Committee member) / School of Molecular Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
The goal of this study is to contribute to the understanding of Mexican-American three- to five-year-old children’s effortful control (EC) and negative emotionality (NE) development by examining whether Mexican-American adolescent mothers’ parenting transacts with their three- to five-year-old children’s EC and NE and by exploring whether mothers’ familism acts as a protective factor. I hypothesized that mothers’ harshness and warmth would transact with EC and NE over time. I further hypothesized that mothers’ familism values would (a) positively predict mothers’ warmth and negatively predict mothers’ harshness, and (b) act as a buffer between low EC and high NE, and high harshness and low warmth. These hypotheses were tested within a sample of Mexican-American adolescent mother-child dyads (N = 204) and assessed longitudinally when children were 36, 48, and 60 months. Mothers were predominantly first generation (i.e., mothers’ parents were born in Mexico; 67%) and spoke English (65%). When children were 36 months, average family income (i.e., wages, public assistance, food stamps) was $24,715 (SD = $19,545) and mothers had started community college (13%) or completed high school/GED (30%), 11th grade (19%), 10th grade (8%), or less than 9th grade (14%). In this sample, transactions between harshness or warmth and EC or NE were not found, but a bidirectional association between NE and harshness was found. Familism marginally negatively predicted harshness, but not warmth. Familism moderated the relation between NE and harshness such that there was only a negative relation between NE and harshness when familism was high. However, familism did not moderate the relations between NE and warmth, or EC and harshness or warmth. The results of this study are discussed with respect to (a) current methodological limitations in the field, such as the need to test or develop parent-report measures of Mexican-American children’s temperament and value-driven socialization goals, (b) future avenues for research, such as person-centered studies of clusters of mothers’ values and how those relate to clusters of parenting behaviors, and (c) implications for interventions addressing parenting behavior of adolescent mothers.
ContributorsBerger, Rebecca H (Author) / Wilkens, Natalie (Thesis advisor) / Spinrad, Tracy (Committee member) / Updegraff, Kimberly (Committee member) / Crnic, Keith (Committee member) / Arizona State University (Publisher)
Created2018
Description
Rapidly growing research on mothers’ perinatal depression, has demonstrated significant links among mothers’ depressive symptoms during pregnancy and the first year postpartum, their parenting, and multiple aspects of children’s development. This prospective longitudinal study contributes to research on mothers’ perinatal depression by examining the mechanisms by which maternal perinatal depression is associated with children’s adjustment early in development in a sample of 204 Mexican-origin adolescent mothers (Mage at Wave 1 = 16.80, SD = 1.0) and their children (58% boys). I expected that adolescent mothers’ negative parenting behaviors would mediate the associations between mothers’ perinatal depressive symptoms and three child outcomes: internalizing symptoms, externalizing behaviors, and cognitive ability. I further hypothesized that mothers’ perceived social support from their family would modify the extent to which mothers’ perinatal depressive symptoms negatively impact their parenting behaviors and their children’s developmental outcomes. Mothers reported on their own depressive symptoms, their perceived social support from their family and their children’s internalizing and externalizing problems; negative parenting was assessed using observational methods; and children’s cognitive ability was assessed using standardized developmental assessments. In this sample, adolescent mothers’ negative parenting behaviors did not significantly mediate the relations between mothers’ perinatal depression and children’s developmental outcomes. Further, perceived social support did not significantly buffer the effects of mothers’ perinatal depression on mothers’ negative parenting or children’s developmental outcomes. However, in line with hypotheses, results indicated that mothers’ prenatal depression had a wider impact on children’s adjustment outcomes than mothers’ postpartum depression, which appeared more specific to children’s internalizing problems. Discussion focuses on implications for intervention addressing adolescent mothers’ perinatal depression, as well as the need to continue to explore protective factors that have the potential to disrupt the negative intergenerational transmission of risks.
ContributorsSeay, Danielle M (Author) / Elam, Kit (Thesis advisor) / Iida, Masumi (Thesis advisor) / Thompson, Marilyn (Committee member) / Wilkens, Natalie (Committee member) / Arizona State University (Publisher)
Created2019
Description
Purpose: The goal of this study was to examine how vocabulary size and inhibitory control affect word learning in bilingual (English-Spanish) children with hearing loss. Experiment 1 examined whether children with larger vocabularies learn and retain more words than children with smaller vocabularies. Experiment 2 examined whether children with better inhibitory control learn and remember more words than children with poorer inhibitory control. In addition, monolingual and bilingual children with and without hearing loss were compared on word learning and inhibitory control tasks.
Method: Seventy-three children between 8 and 12 years of age participated in the study. Forty children had normal hearing (20 monolingual and 20 bilingual) and 33 had hearing loss (20 monolingual and 13 bilingual). For Experiment 1, children completed a receptive vocabulary test in English and Spanish and three word learning tasks consisting of a training and a retention component in English, Spanish, and Arabic. For Experiment 2, children completed the flanker task for inhibitory control.
Results: In Experiment 1, larger total (English + Spanish) receptive vocabularies were predictive of better word training outcomes in all languages and better Spanish word retention, after controlling for age, degree of hearing loss, and maternal education. Children with hearing loss performed more poorly in Spanish and Arabic word training and retention than children with normal hearing. No differences were observed between children with normal hearing and hearing loss in English word learning. In Experiment 2, inhibitory control only predicted English retention outcomes. Children with hearing loss showed poorer inhibitory control than hearing peers. No differences were observed between monolingual and bilingual children, with and without hearing loss, in word learning or inhibitory control.
Conclusions: Language experience (measured by total vocabulary size) helps children learn new words and therefore children with hearing loss should receive well-fitted hearing aids and school accommodations to provide them with access to spoken language. Bilingual exposure does not impair nor facilitate word learning. Bilingual children showed similar difficulties with word learning and inhibitory control as monolingual peers with hearing loss. Hearing loss, probably via language deprivation, has broad effects on children’s executive function skills.
Method: Seventy-three children between 8 and 12 years of age participated in the study. Forty children had normal hearing (20 monolingual and 20 bilingual) and 33 had hearing loss (20 monolingual and 13 bilingual). For Experiment 1, children completed a receptive vocabulary test in English and Spanish and three word learning tasks consisting of a training and a retention component in English, Spanish, and Arabic. For Experiment 2, children completed the flanker task for inhibitory control.
Results: In Experiment 1, larger total (English + Spanish) receptive vocabularies were predictive of better word training outcomes in all languages and better Spanish word retention, after controlling for age, degree of hearing loss, and maternal education. Children with hearing loss performed more poorly in Spanish and Arabic word training and retention than children with normal hearing. No differences were observed between children with normal hearing and hearing loss in English word learning. In Experiment 2, inhibitory control only predicted English retention outcomes. Children with hearing loss showed poorer inhibitory control than hearing peers. No differences were observed between monolingual and bilingual children, with and without hearing loss, in word learning or inhibitory control.
Conclusions: Language experience (measured by total vocabulary size) helps children learn new words and therefore children with hearing loss should receive well-fitted hearing aids and school accommodations to provide them with access to spoken language. Bilingual exposure does not impair nor facilitate word learning. Bilingual children showed similar difficulties with word learning and inhibitory control as monolingual peers with hearing loss. Hearing loss, probably via language deprivation, has broad effects on children’s executive function skills.
Contributorsde Diego Lazaro, Beatriz (Author) / Pittman, Andrea L (Thesis advisor) / Restrepo, María A (Thesis advisor) / Benitez, Viridiana (Committee member) / Wilkens, Natalie (Committee member) / Arizona State University (Publisher)
Created2019