Matching Items (124)
Description

Every season from September to March in Taiji, Japan, around 23,000 dolphins, and other small cetaceans are slaughtered or sold to dolphinariums in the name of a 400-year-old tradition. The word ‘tradition’ is often used to rationalize and justify the terrible acts of animal cruelty, as seen in many countries

Every season from September to March in Taiji, Japan, around 23,000 dolphins, and other small cetaceans are slaughtered or sold to dolphinariums in the name of a 400-year-old tradition. The word ‘tradition’ is often used to rationalize and justify the terrible acts of animal cruelty, as seen in many countries such as bullfighting in Spain, fox hunting in Britain, Thanksgiving in America, and drive hunting in Japan. However, just because something is deemed as a tradition, does not mean it should not be challenged and judged against the standards of morality. Whale and dolphin hunting has stopped becoming a proud cultural tradition of small-scale subsistence whaling and has become a business run on wholesale slaughter and the exploitation of another species. The disconnect between the past and present has led to an evil distortion of the past.
However, this event cannot simply be explained by blaming solely greed and selfishness for driving this long-lasting tradition. By analyzing poems by Misuzu Kaneko, early hunting methods, memorial services, and graves built in the past and comparing them to the current hunting methods, dolphin shows, and the Taiji Whale Museum, one can determine the variety of factors driving these actions and find the point in time when the intentions of these practices shifted. By having a better understanding of the past and the present, one can follow a once-proud tradition becoming a source to justify unethical and cruel behavior.

ContributorsMinotto, Aoi (Author) / Middel, Ariane (Thesis director) / Hagen, Bjoern (Committee member) / Arts, Media and Engineering Sch T (Contributor) / School of Music, Dance and Theatre (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Description

This study examines the impact of spatial landscape configuration (e.g., clustered, dispersed) on land-surface temperatures (LST) over Phoenix, Arizona, and Las Vegas, Nevada, USA. We classified detailed land-cover types via object-based image analysis (OBIA) using Geoeye-1 at 3-m resolution (Las Vegas) and QuickBird at 2.4-m resolution (Phoenix). Spatial autocorrelation (local

This study examines the impact of spatial landscape configuration (e.g., clustered, dispersed) on land-surface temperatures (LST) over Phoenix, Arizona, and Las Vegas, Nevada, USA. We classified detailed land-cover types via object-based image analysis (OBIA) using Geoeye-1 at 3-m resolution (Las Vegas) and QuickBird at 2.4-m resolution (Phoenix). Spatial autocorrelation (local Moran’s I ) was then used to test for spatial dependence and to determine how clustered or dispersed points were arranged. Next, we used Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) data acquired over Phoenix (daytime on 10 June and nighttime on 17 October 2011) and Las Vegas (daytime on 6 July and nighttime on 27 August 2005) to examine day- and nighttime LST with regard to the spatial arrangement of anthropogenic and vegetation features. Local Moran’s I values of each land-cover type were spatially correlated to surface temperature. The spatial configuration of grass and trees shows strong negative correlations with LST, implying that clustered vegetation lowers surface temperatures more effectively. In contrast, clustered spatial arrangements of anthropogenic land-cover types, especially impervious surfaces and open soil, elevate LST. These findings suggest that city planners and managers should, where possible, incorporate clustered grass and trees to disperse unmanaged soil and paved surfaces, and fill open unmanaged soil with vegetation. Our findings are in line with national efforts to augment and strengthen green infrastructure, complete streets, parking management, and transit-oriented development practices, and reduce sprawling, unwalkable housing development.

ContributorsMyint, Soe Win (Author) / Zheng, Baojuan (Author) / Talen, Emily (Author) / Fan, Chao (Author) / Kaplan, Shari (Author) / Middel, Ariane (Author) / Smith, Martin (Author) / Huang, Huei-Ping (Author) / Brazel, Anthony J. (Author)
Created2015-06-29
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Description

This study seeks to determine the role of land architecture—the composition and configuration of land cover—as well as cadastral/demographic/economic factors on land surface temperature (LST) and the surface urban heat island effect of Phoenix, Arizona. It employs 1 m National Agricultural Imagery Program data of land-cover with 120mLandsat-derived land surface

This study seeks to determine the role of land architecture—the composition and configuration of land cover—as well as cadastral/demographic/economic factors on land surface temperature (LST) and the surface urban heat island effect of Phoenix, Arizona. It employs 1 m National Agricultural Imagery Program data of land-cover with 120mLandsat-derived land surface temperature, decomposed to 30 m, a new measure of configuration, the normalized moment of inertia, and U.S. Census data to address the question for two randomly selected samples comprising 523 and 545 residential neighborhoods (census blocks) in the city. The results indicate that, contrary to most other studies, land configuration has a stronger influence on LST than land composition. In addition, both land configuration and architecture combined with cadastral, demographic, and economic variables, capture a significant amount of explained variance in LST. The results indicate that attention to land architecture in the development of or reshaping of neighborhoods may ameliorate the summer extremes in LST.

ContributorsLi, Xiaoxiao (Author) / Li, Wenwen (Author) / Middel, Ariane (Author) / Harlan, Sharon L. (Author) / Brazel, Anthony J. (Author) / Turner II, B. L. (Author)
Created2015-12-29
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Description

Objectives: To provide novel quantification and advanced measurements of surface temperatures (Ts) in playgrounds, employing multiple scales of data, and provide insight into hot-hazard mitigation techniques and designs for improved environmental and public health.

Methods: We conduct an analysis of Ts in two Metro-Phoenix playgrounds at three scales: neighborhood (1 km

Objectives: To provide novel quantification and advanced measurements of surface temperatures (Ts) in playgrounds, employing multiple scales of data, and provide insight into hot-hazard mitigation techniques and designs for improved environmental and public health.

Methods: We conduct an analysis of Ts in two Metro-Phoenix playgrounds at three scales: neighborhood (1 km resolution), microscale (6.8 m resolution), and touch-scale (1 cm resolution). Data were derived from two sources: airborne remote sensing (neighborhood and microscale) and in situ (playground site) infrared Ts (touch-scale). Metrics of surface-to-air temperature deltas (Ts–a) and scale offsets (errors) are introduced.

Results: Select in situ Ts in direct sunlight are shown to approach or surpass values likely to result in burns to children at touch-scales much finer than Ts resolved by airborne remote sensing. Scale offsets based on neighbourhood and microscale ground observations are 3.8 ◦C and 7.3 ◦C less than the Ts–a at the 1 cm touch-scale, respectively, and 6.6 ◦C and 10.1 ◦C lower than touch-scale playground equipment Ts, respectively. Hence, the coarser scales underestimate high Ts within playgrounds. Both natural (tree) and artificial (shade sail) shade types are associated with significant reductions in Ts.

Conclusions: A scale mismatch exists based on differing methods of urban Ts measurement. The sub-meter touch-scale is the spatial scale at which data must be collected and policies of urban landscape design and health must be executed in order to mitigate high Ts in high-contact environments such as playgrounds. Shade implementation is the most promising mitigation technique to reduce child burns, increase park usability, and mitigate urban heating.

ContributorsVanos, Jennifer K. (Author) / Middel, Ariane (Author) / McKercher, Grant R. (Author) / Kuras, Evan R. (Author) / Ruddell, Benjamin L. (Author)
Created2015-11-10
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Description
In oxygenic photosynthesis, Photosystem I (PSI) and Photosystem II (PSII) are two transmembrane protein complexes that catalyze the main step of energy conversion; the light induced charge separation that drives an electron transfer reaction across the thylakoid membrane. Current knowledge of the structure of PSI and PSII is based on

In oxygenic photosynthesis, Photosystem I (PSI) and Photosystem II (PSII) are two transmembrane protein complexes that catalyze the main step of energy conversion; the light induced charge separation that drives an electron transfer reaction across the thylakoid membrane. Current knowledge of the structure of PSI and PSII is based on three structures: PSI and PSII from the thermophilic cyanobacterium Thermosynechococcus elonagatus and the PSI/light harvesting complex I (PSI-LHCI) of the plant, Pisum sativum. To improve the knowledge of these important membrane protein complexes from a wider spectrum of photosynthetic organisms, photosynthetic apparatus of the thermo-acidophilic red alga, Galdieria sulphuraria and the green alga, Chlamydomonas reinhardtii were studied. Galdieria sulphuraria grows in extreme habitats such as hot sulfur springs with pH values from 0 to 4 and temperatures up to 56°C. In this study, both membrane protein complexes, PSI and PSII were isolated from this organism and characterized. Ultra-fast fluorescence spectroscopy and electron microscopy studies of PSI-LHCI supercomplexes illustrate how this organism has adapted to low light environmental conditions by tightly coupling PSI and LHC, which have not been observed in any organism so far. This result highlights the importance of structure-function relationships in different ecosystems. Galdieria sulphuraria PSII was used as a model protein to show the amenability of integral membrane proteins to top-down mass spectrometry. G.sulphuraria PSII has been characterized with unprecedented detail with identification of post translational modification of all the PSII subunits. This study is a technology advancement paving the way for the usage of top-down mass spectrometry for characterization of other large integral membrane proteins. The green alga, Chlamydomonas reinhardtii is widely used as a model for eukaryotic photosynthesis and results from this organism can be extrapolated to other eukaryotes, especially agricultural crops. Structural and functional studies on the PSI-LHCI complex of C.reinhardtii grown under high salt conditions were studied using ultra-fast fluorescence spectroscopy, circular dichroism and MALDI-TOF. Results revealed that pigment-pigment interactions in light harvesting complexes are disrupted and the acceptor side (ferredoxin docking side) is damaged under high salt conditions.
ContributorsThangaraj, Balakumar (Author) / Fromme, Petra (Thesis advisor) / Shock, Everett (Committee member) / Chen, Julian (Committee member) / Arizona State University (Publisher)
Created2010
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Description
Tempe Town Lake is the site of fifteen years’ worth of chemical data collection by ASU researchers. In 2018 the dataSONDE, an instrument capable of measuring different water quality parameters every thirty minutes for a month at a time was installed in the lake. The SONDE has the potential to

Tempe Town Lake is the site of fifteen years’ worth of chemical data collection by ASU researchers. In 2018 the dataSONDE, an instrument capable of measuring different water quality parameters every thirty minutes for a month at a time was installed in the lake. The SONDE has the potential to completely reduce the need for sampling by hand. Before the SONDE becomes the sole means of gathering data, it is important to verify its accuracy. In this study, the measurements gathered by the SONDE (pH, dissolved oxygen, temperature, conductivity and colored dissolved organic matter) were compared to measurements gathered using the verified methods from the past fifteen years.
ContributorsSauer, Elinor Rayne (Author) / Hartnett, Hilairy (Thesis director) / Glaser, Donald (Committee member) / Shock, Everett (Committee member) / Historical, Philosophical & Religious Studies (Contributor) / School of Molecular Sciences (Contributor) / School of Life Sciences (Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2020-12
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Description

Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We

Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.

ContributorsOberthuer, Dominik (Author) / Knoska, Juraj (Author) / Wiedorn, Max O. (Author) / Beyerlein, Kenneth R. (Author) / Bushnell, David A. (Author) / Kovaleva, Elena G. (Author) / Heymann, Michael (Author) / Gumprecht, Lars (Author) / Kirian, Richard (Author) / Barty, Anton (Author) / Mariani, Valerio (Author) / Tolstikova, Aleksandra (Author) / Adriano, Luigi (Author) / Awel, Salah (Author) / Barthelmess, Miriam (Author) / Dorner, Katerina (Author) / Xavier, P. Lourdu (Author) / Yefanov, Oleksandr (Author) / James, Daniel (Author) / Nelson, Garrett (Author) / Wang, Dingjie (Author) / Calvey, George (Author) / Chen, Yujie (Author) / Schmidt, Andrea (Author) / Szczepek, Michael (Author) / Frielingsdorf, Stefan (Author) / Lenz, Oliver (Author) / Snell, Edward (Author) / Robinson, Philip J. (Author) / Sarler, Bozidar (Author) / Belsak, Grega (Author) / Macek, Marjan (Author) / Wilde, Fabian (Author) / Aquila, Andrew (Author) / Boutet, Sebastien (Author) / Liang, Mengning (Author) / Hunter, Mark S. (Author) / Scheerer, Patrick (Author) / Lipscomb, John D. (Author) / Weierstall, Uwe (Author) / Kornberg, Roger D. (Author) / Spence, John (Author) / Pollack, Lois (Author) / Chapman, Henry N. (Author) / Bajt, Sasa (Author) / College of Liberal Arts and Sciences (Contributor) / Department of Physics (Contributor)
Created2017-03-16
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Description

X-ray free-electron lasers provide novel opportunities to conduct single particle analysis on nanoscale particles. Coherent diffractive imaging experiments were performed at the Linac Coherent Light Source (LCLS), SLAC National Laboratory, exposing single inorganic core-shell nanoparticles to femtosecond hard-X-ray pulses. Each facetted nanoparticle consisted of a crystalline gold core and a

X-ray free-electron lasers provide novel opportunities to conduct single particle analysis on nanoscale particles. Coherent diffractive imaging experiments were performed at the Linac Coherent Light Source (LCLS), SLAC National Laboratory, exposing single inorganic core-shell nanoparticles to femtosecond hard-X-ray pulses. Each facetted nanoparticle consisted of a crystalline gold core and a differently shaped palladium shell. Scattered intensities were observed up to about 7 nm resolution. Analysis of the scattering patterns revealed the size distribution of the samples, which is consistent with that obtained from direct real-space imaging by electron microscopy. Scattering patterns resulting from single particles were selected and compiled into a dataset which can be valuable for algorithm developments in single particle scattering research.

ContributorsLi, Xuanxuan (Author) / Chiu, Chun-Ya (Author) / Wang, Hsiang-Ju (Author) / Kassemeyer, Stephan (Author) / Botha, Sabine (Author) / Shoeman, Robert L. (Author) / Lawrence, Robert (Author) / Kupitz, Christopher (Author) / Kirian, Richard (Author) / James, Daniel (Author) / Wang, Dingjie (Author) / Nelson, Garrett (Author) / Messerschmidt, Marc (Author) / Boutet, Sebastien (Author) / Williams, Garth J. (Author) / Hartman, Elisabeth (Author) / Jafarpour, Aliakbar (Author) / Foucar, Lutz M. (Author) / Barty, Anton (Author) / Chapman, Henry (Author) / Liang, Mengning (Author) / Menzel, Andreas (Author) / Wang, Fenglin (Author) / Basu, Shibom (Author) / Fromme, Raimund (Author) / Doak, R. Bruce (Author) / Fromme, Petra (Author) / Weierstall, Uwe (Author) / Huang, Michael H. (Author) / Spence, John (Author) / Schlichting, Ilme (Author) / Hogue, Brenda (Author) / Liu, Haiguang (Author) / ASU Biodesign Center Immunotherapy, Vaccines and Virotherapy (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / College of Liberal Arts and Sciences (Contributor) / School of Molecular Sciences (Contributor) / Department of Physics (Contributor) / School of Life Sciences (Contributor)
Created2017-04-11
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Description

Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of

Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 μm diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 Ångström were recorded. The diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.

ContributorsMunke, Anna (Author) / Andreasson, Jakob (Author) / Aquila, Andrew (Author) / Awel, Salah (Author) / Ayyer, Kartik (Author) / Barty, Anton (Author) / Bean, Richard J. (Author) / Berntsen, Peter (Author) / Bielecki, Johan (Author) / Boutet, Sebastien (Author) / Bucher, Maximilian (Author) / Chapman, Henry N. (Author) / Daurer, Benedikt J. (Author) / DeMirci, Hasan (Author) / Elser, Veit (Author) / Fromme, Petra (Author) / Hajdu, Janos (Author) / Hantke, Max F. (Author) / Higashiura, Akifumi (Author) / Hogue, Brenda (Author) / Hosseinizadeh, Ahmad (Author) / Kim, Yoonhee (Author) / Kirian, Richard (Author) / Reddy, Hemanth K. N. (Author) / Lan, Ti-Yen (Author) / Larsson, Daniel S. D. (Author) / Liu, Haiguang (Author) / Loh, N. Duane (Author) / Maia, Filipe R. N. C. (Author) / Mancuso, Adrian P. (Author) / Muhlig, Kerstin (Author) / Nakagawa, Atsushi (Author) / Nam, Daewoong (Author) / Nelson, Garrett (Author) / Nettelblad, Carl (Author) / Okamoto, Kenta (Author) / Ourmazd, Abbas (Author) / Rose, Max (Author) / van der Schot, Gijs (Author) / Schwander, Peter (Author) / Seibert, M. Marvin (Author) / Sellberg, Jonas A. (Author) / Sierra, Raymond G. (Author) / Song, Changyong (Author) / Svenda, Martin (Author) / Timneanu, Nicusor (Author) / Vartanyants, Ivan A. (Author) / Westphal, Daniel (Author) / Wiedom, Max O. (Author) / Williams, Garth J. (Author) / Xavier, Paulraj Lourdu (Author) / Soon, Chun Hong (Author) / Zook, James (Author) / College of Liberal Arts and Sciences (Contributor, Contributor) / School of Molecular Sciences (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / School of Life Sciences (Contributor) / Infectious Diseases and Vaccinology (Contributor) / Department of Physics (Contributor)
Created2016-08-01
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Description
Serial femtosecond X-ray crystallography (SFX) using an X-ray free electron laser (XFEL) is a recent advancement in structural biology for solving crystal structures of challenging membrane proteins, including G-protein coupled receptors (GPCRs), which often only produce microcrystals. An XFEL delivers highly intense X-ray pulses of femtosecond duration short enough to

Serial femtosecond X-ray crystallography (SFX) using an X-ray free electron laser (XFEL) is a recent advancement in structural biology for solving crystal structures of challenging membrane proteins, including G-protein coupled receptors (GPCRs), which often only produce microcrystals. An XFEL delivers highly intense X-ray pulses of femtosecond duration short enough to enable the collection of single diffraction images before significant radiation damage to crystals sets in. Here we report the deposition of the XFEL data and provide further details on crystallization, XFEL data collection and analysis, structure determination, and the validation of the structural model. The rhodopsin-arrestin crystal structure solved with SFX represents the first near-atomic resolution structure of a GPCR-arrestin complex, provides structural insights into understanding of arrestin-mediated GPCR signaling, and demonstrates the great potential of this SFX-XFEL technology for accelerating crystal structure determination of challenging proteins and protein complexes.
ContributorsZhou, X. Edward (Author) / Gao, Xiang (Author) / Barty, Anton (Author) / Kang, Yanyong (Author) / He, Yuanzheng (Author) / Liu, Wei (Author) / Ishchenko, Andrii (Author) / White, Thomas A. (Author) / Yefanov, Oleksandr (Author) / Han, Gye Won (Author) / Xu, Qingping (Author) / de Waal, Parker W. (Author) / Suino-Powell, Kelly M. (Author) / Boutet, Sebastien (Author) / Williams, Garth J. (Author) / Wang, Meitian (Author) / Li, Dianfan (Author) / Caffrey, Martin (Author) / Chapman, Henry N. (Author) / Spence, John (Author) / Fromme, Petra (Author) / Weierstall, Uwe (Author) / Stevens, Raymond C. (Author) / Cherezov, Vadim (Author) / Melcher, Karsten (Author) / Xu, H. Eric (Author) / College of Liberal Arts and Sciences (Contributor) / School of Molecular Sciences (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / Department of Physics (Contributor)
Created2016-04-12