Diisobutylene maleic acid, or DIBMA, offers a novel approach to integral membrane protein extraction without requiring the use of detergent. This copolymer extracts the protein along with the surrounding lipids, creating native nanodiscs. This method of solubilization is the preferred method, as traditional detergent solubilization can possibly alter the structural and functional integrity of the membrane protein. DIBMA solubilization, on the other hand, is able to create a more stable environment for the integral membrane protein, while allowing purification through commonly used chromatography methods similar to established detergent solubilization protocols. In this project, we study the ability of DIBMA to isolate the integral membrane protein, chloroplast ATP synthase, without the use of detergents.
With the increase in the severity of drought conditions in the Southwest region of the U.S. paired with rising temperatures, it is becoming increasingly important to look at the systems used to keep people cool in hot-arid cities like Tempe, Arizona. Outdoor misting systems are often deployed by businesses. These systems rely on the evaporative cooling effect of water. This study examines the relationship between misting droplet size, water usage, and thermal comfort using low-pressure misting systems, tested within hot and dry conditions representative of the arid U.S. southwest. A model misting system using three nozzle orifice sizes was set up in a controlled heat chamber environment (starting baseline conditions of 40°C air temperature and 15 % relative humidity). Droplet size was measured using water-reactive paper, while water use was determined based on weight-change measurements. These measurements were paired with temperature and humidity measurements observed in several locations around the chamber to allow for a spatial analysis. Thermal comfort is determined based on psychrometric changes (temperature and absolute humidity) within the room. On average, air temperatures decreased between 2 to 4°C depending on nozzle size and sensor location. The 0.4 mm nozzle had a decent spread across the heat chamber and balanced water usage and effectiveness well. Limitations within the study showed ventilation is important for an effective system, corroborating other studies findings and suggesting that adding air circulation could improve evaporation and comfort and thus effectiveness. Finally, visual cues, such as wetted surfaces, can signal businesses to change nozzle sizes and/or make additional modifications to the system area.