This is a theatrical script for a personal narrative driven solo performance piece that focuses on advocating for the value and importance of pursuing civil discourse and intellectual humility in order to change people’s minds and find agreement among disagreement. It tracks a personal story of developing various, conflicting, worldviews and exploring how this conflict can be dealt with, as well as how it can inform dealing with others.

In nature, some animals have an exoskeleton that provides protection, strength, and stability to the organism, but in engineering, an exoskeleton refers to a device that augments or aids human ability. Since the 1890s, engineers have been designing exoskeletal devices, and conducting research into the possible uses of such devices. These bio-inspired mechanisms do not necessarily relate to a robotic device, though since the 1900s, robotic principles have been applied to the design of exoskeletons making their development a subfield in robotic research. There are different multiple types of exoskeletons that target different areas of the human body, and the targeted area depends on the need of the device. Usually, the devices are developed for medical or military usage; for this project, the focus is on medical development of an automated elbow joint to assist in rehabilitation. This project is being developed for therapeutic purposes in conjunction between Arizona State University and Mayo Clinic. Because of the nature of this project, I am responsible for the development of a lightweight brace that could be applied to the elbow joint that was designed by Dr. Kevin Hollander. In this project, my research centered on the use of the Wilmer orthosis brace design, and its possible application to the exoskeleton elbow being developed for Mayo Clinic. This brace is a lightweight solution that provides extra comfort to the user.

The HLA, Human Leukocyte Antigens, are encoded by a polymorphic set of genes where even a single base change can impact the function of the body’s immune response to foreign antigens [1]. Although many methods exist to type these alleles using whole-genome sequencing (WGS), few can use RNA sequencing (RNA-seq) to show the functional expression of the alleles with its inconsistency in coverage, and none of these allow for novel allele discovery. We present an approach using partially ordered graphs to project sequenced data onto the known alleles allowing for accurate and efficient typing of the HLA genes with flexibility for discovering new alleles and tolerance for poor sequence quality. This graph-guided approach to assembling and typing the HLA genes from RNA-seq has applications throughout precision medicine, facilitating the prevention and treatment of autoimmune diseases where allele expression can change. It is also a necessary step for determining donors for organ transplants with the least likelihood of rejection. This novel approach of combining database matching with partially ordered graphs for assembling genetic sequences of RNA-seq data could be applied towards typing other alleles.