Human protein diversity arises as a result of alternative splicing, single nucleotide polymorphisms (SNPs) and posttranslational modifications. Because of these processes, each protein can exists as multiple variants in vivo. Tailored strategies are needed to study these protein variants and understand their role in health and disease. In this work we utilized quantitative mass spectrometric immunoassays to determine the protein variants concentration of beta-2-microglobulin, cystatin C, retinol binding protein, and transthyretin, in a population of 500 healthy individuals. Additionally, we determined the longitudinal concentration changes for the protein variants from four individuals over a 6 month period. Along with the native forms of the four proteins, 13 posttranslationally modified variants and 7 SNP-derived variants were detected and their concentration determined. Correlations of the variants concentration with geographical origin, gender, and age of the individuals were also examined. This work represents an important step toward building a catalog of protein variants concentrations and examining their longitudinal changes.
The ecological impact of energy production and consumption is often relegated in analytical accounts of the evolution of energy systems, where production and consumption patterns are analysed as the interaction of social, economic and technological factors. Ecological and social–ecological dynamics are, we argue, critical in the context of imperatives for access to modern energy services that are inadequate for significant sections of the world's population. The ecological impacts of energy use are often analysed as a set of externalities, many of which are uncertain or unquantifiable, particularly if they stem from earth system change such as anthropogenic climate change. Here we outline the benefits from analysing energy systems as social–ecological systems. We review the extensive literature from ecology and resilience theories, and compare the analytical domains, major findings and emphasis of social–ecological systems with socio-technical transition research. We illustrate these differences with the example of the multi-scale impacts of biofuel expansion. We show that social–ecological systems research combines analysis of interactions with ecological systems and power relations between actors in energy systems, and has the potential to do so across production, distribution and consumption domains whilst illustrating the dynamics of such energy systems, identifying potential trade-offs and regime shifts.
Faced with numerous seemingly intractable social and environmental challenges, many scholars and practitioners are increasingly interested in understanding how to actively engage and transform the existing systems holding such problems in place. Although a variety of analytical models have emerged in recent years, most emphasize either the social or ecological elements of such transformations rather than their coupled nature. To address this, first we have presented a definition of the core elements of a social-ecological system (SES) that could potentially be altered in a transformation. Second, we drew on insights about transformation from three branches of literature focused on radical change, i.e., social movements, socio-technical transitions, and social innovation, and gave consideration to the similarities and differences with the current studies by resilience scholars. Drawing on these findings, we have proposed a framework that outlines the process and phases of transformative change in an SES. Future research will be able to utilize the framework as a tool for analyzing the alteration of social-ecological feedbacks, identifying critical barriers and leverage points and assessing the outcome of social-ecological transformations.