Matching Items (309)
Description
This project uses the tintype process to create contemporary portraits. The tintype is a nineteenth century photographic process that creates a direct positive on a sheet of metal. The images were created using the same process from the 1800s, as well as a more contemporary method. The natural imperfections caused by both processes were used to help examine the intimacy and emotion present in the portraits.
ContributorsRichey, Jordyn Elizabeth (Author) / Danh, Binh (Thesis director) / Schneider, Betsy (Committee member) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor) / School of Art (Contributor)
Created2014-05
Description
Pathogenic Gram-negative bacteria employ a variety of molecular mechanisms to combat host defenses. Two-component regulatory systems (TCR systems) are the most ubiquitous signal transduction systems which regulate many genes required for virulence and survival of bacteria. In this study, I analyzed different TCR systems in two clinically-relevant Gram-negative bacteria, i.e., oral pathogen Porphyromonas gingivalis and enterobacterial Escherichia coli. P. gingivalis is a major causative agent of periodontal disease as well as systemic illnesses, like cardiovascular disease. A microarray study found that the putative PorY-PorX TCR system controls the secretion and maturation of virulence factors, as well as loci involved in the PorSS secretion system, which secretes proteinases, i.e., gingipains, responsible for periodontal disease. Proteomic analysis (SILAC) was used to improve the microarray data, reverse-transcription PCR to verify the proteomic data, and primer extension assay to determine the promoter regions of specific PorX regulated loci. I was able to characterize multiple genetic loci regulated by this TCR system, many of which play an essential role in hemagglutination and host-cell adhesion, and likely contribute to virulence in this bacterium. Enteric Gram-negative bacteria must withstand many host defenses such as digestive enzymes, low pH, and antimicrobial peptides (AMPs). The CpxR-CpxA TCR system of E. coli has been extensively characterized and shown to be required for protection against AMPs. Most recently, this TCR system has been shown to up-regulate the rfe-rff operon which encodes genes involved in the production of enterobacterial common antigen (ECA), and confers protection against a variety of AMPs. In this study, I utilized primer extension and DNase I footprinting to determine how CpxR regulates the ECA operon. My findings suggest that CpxR modulates transcription by directly binding to the rfe promoter. Multiple genetic and biochemical approaches were used to demonstrate that specific TCR systems contribute to regulation of virulence factors and resistance to host defenses in P. gingivalis and E. coli, respectively. Understanding these genetic circuits provides insight into strategies for pathogenesis and resistance to host defenses in Gram negative bacterial pathogens. Finally, these data provide compelling potential molecular targets for therapeutics to treat P. gingivalis and E. coli infections.
ContributorsLeonetti, Cori (Author) / Shi, Yixin (Thesis advisor) / Stout, Valerie (Committee member) / Nickerson, Cheryl (Committee member) / Sandrin, Todd (Committee member) / Arizona State University (Publisher)
Created2013
Description
The study of bacterial resistance to antimicrobial peptides (AMPs) is a significant area of interest as these peptides have the potential to be developed into alternative drug therapies to combat microbial pathogens. AMPs represent a class of host-mediated factors that function to prevent microbial infection of their host and serve as a first line of defense. To date, over 1,000 AMPs of various natures have been predicted or experimentally characterized. Their potent bactericidal activities and broad-based target repertoire make them a promising next-generation pharmaceutical therapy to combat bacterial pathogens. It is important to understand the molecular mechanisms, both genetic and physiological, that bacteria employ to circumvent the bactericidal activities of AMPs. These understandings will allow researchers to overcome challenges posed with the development of new drug therapies; as well as identify, at a fundamental level, how bacteria are able to adapt and survive within varied host environments. Here, results are presented from the first reported large scale, systematic screen in which the Keio collection of ~4,000 Escherichia coli deletion mutants were challenged against physiologically significant AMPs to identify genes required for resistance. Less than 3% of the total number of genes on the E. coli chromosome was determined to contribute to bacterial resistance to at least one AMP analyzed in the screen. Further, the screen implicated a single cellular component (enterobacterial common antigen, ECA) and a single transporter system (twin-arginine transporter, Tat) as being required for resistance to each AMP class. Using antimicrobial resistance as a tool to identify novel genetic mechanisms, subsequent analyses were able to identify a two-component system, CpxR/CpxA, as a global regulator in bacterial resistance to AMPs. Multiple previously characterized CpxR/A members, as well as members found in this study, were identified in the screen. Notably, CpxR/A was found to transcriptionally regulate the gene cluster responsible for the biosynthesis of the ECA. Thus, a novel genetic mechanism was uncovered that directly correlates with a physiologically significant cellular component that appears to globally contribute to bacterial resistance to AMPs.
ContributorsWeatherspoon-Griffin, Natasha (Author) / Shi, Yixin (Thesis advisor) / Clark-Curtiss, Josephine (Committee member) / Misra, Rajeev (Committee member) / Nickerson, Cheryl (Committee member) / Stout, Valerie (Committee member) / Arizona State University (Publisher)
Created2013
Description
Since the early 1990's, researchers have been looking at intersections between education and music. After a highly popular study correlating listening to Mozart to temporary increases in spatial reasoning, many other researchers tried to find a link between different musical genres and learning outcomes. Using three musical treatments (Pop, classical, silence), this study had subjects (N=34) complete a reading-based task whereupon they were tested on their comprehension. Using a suite of sensors, data was collected to analyze the participants' emotions and affect while they read from an educational psychology textbook. The present study has two major focuses: They detail whether (1) changes in musical condition affect learning outcomes and (2) whether changes in musical condition affect emotional outcomes. The popular conception that listening to classical music makes you smarter was proven false long ago, but there may actually be some merit to using music to assist one in studying. While there were no significant changes in test scores depending on musical condition; frustration levels were significantly lower for those who listened to classical instead of pop music.
ContributorsPaley, Benjamin Henry (Author) / Atkinson, Robert (Thesis director) / Feisst, Sabine (Committee member) / Barrett, The Honors College (Contributor) / School of Music (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor)
Created2015-05
Description
Hepatitis C virus (HCV) is a globally prevalent infection which is a main contributor to the global burden of liver disease. Due to its ability to establish a chronic infection, and the lack of usefulness of traditional neutralizing antibody vaccine design in producing a protective immune response, a preventative vaccine has been notoriously difficult to produce. To overcome this, a vaccine using non-structural protein 3 (NS3) as a target to elicit a T cell specific immune response is thought to be a possible strategy for eliciting a protective immune response against hepatitis C infection. In this paper, a recombinant strain of measles virus (MV) that expresses HCV NS3 protein was analyzed. The replication fitness of this recombinant virus also indicates that this construct replicates at a higher rate than parental measles strain. It is also demonstrated through western blot analysis of protein expression and immunofluorescence that this recombinant virus expresses both the inserted HCV NS3 protein, as well as native measles proteins.
ContributorsWoell, Dana Marie (Author) / Reyes del Valle, Jorge (Thesis director) / Nickerson, Cheryl (Committee member) / Julik, Emily (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor) / School of Human Evolution and Social Change (Contributor)
Created2015-05
Description
This thesis was an analysis of items in the Late Adolescent Home Observation for Measure of the Environment (LA HOME) after the first wave of N = 138 interviews. The purpose of this project was to learn how to utilize a statistical software such as SPSS to analyze items and interpret results. Frequency analysis, inter-rater reliability (IRR), correlation analysis, internal consistency using Cronbach's alpha, and feedback from research assistants were considered when deciding which items should be eliminated from the measure. After running these analyses, ten items were suggested for deletion including: clean, adolescent's room allows for privacy, reference materials, news, family encourages adolescent to think independently, community service, parent knows where adolescent spends time, weekly household responsibilities, school/career planning, and dentist. Future interviews generating a larger sample size as well as discussions and subsequent revisions to the manual will clarify additional items that may be eliminated from the final version of the instrument.
ContributorsMiller, Ava Kathryn (Author) / Bradley, Robert (Thesis director) / Spinrad, Tracy (Committee member) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor)
Created2015-05
Description
Bacteria play a vital role in the world ecosystem, more importantly human health and disease. The capability to differentiate and identify these microorganisms serves as an important research objective. In past years, separations-based approaches have served as a way to observe and identify bacteria based on their characteristics. Gradient insulator dielectrophoresis (g-iDEP) provides benefits in identifying serotypes of a single species with precise separation. Separation of Staphylococcus epidermidis in a single g-iDEP microchannel is conducted exploiting their electrophoretic and electrokinetic properties. The cells were captured and concentrated at gates with interacting forces within the microchannel to clearly distinguish between the two strains. These results provide support for g-iDEP serving as a separating method and, furthermore, future clinical applications.
ContributorsDavis, Paige Elizabeth (Author) / Hayes, Mark (Thesis director) / Borges, Chad (Committee member) / Jones, Paul (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor)
Created2015-05
Description
In the United States, many new cases of type I diabetes appear among youth. Upon diagnosis, many patients experience psychosocial issues in addition to physical issues, including depression, anxiety, and poor self-esteem. Diabetes educators have found that it is important to form interpersonal connections and trust with their adolescent patients. One tool that may be particularly useful for diabetes educators to implement is art therapy, which combines creativity and problem solving in a practical manner. Art therapy may be particularly helpful for individuals with type 1 diabetes because of the great cognitive and emotional changes, which occur during adolescence. In order for caretakers and educators to implement tenants of art therapy, it is helpful to have a medium, such as an art journal, which provides a foundation on which to process the emotions and thoughts the adolescent is experiencing as they process diabetes and their feelings throughout diagnosis. Keywords: art therapy, type I diabetes
ContributorsFowle, Mikaelah Jenee (Author) / Bodman, Denise (Thesis director) / Rogers, Jennifer (Committee member) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor)
Created2015-05
Description
In the mid-1970s, social scientists began observing marital dyad conversations in laboratory settings with the hope of determining which observable features best discriminate couples who report being either satisfied or unsatisfied with their relationship. These studies continued until about a decade ago when, in addition to increasing laboratory costs slowing the pace of new data collection, researchers realized that distressed couples were easier to quantitatively describe than nondistressed couples. Specifically, distressed couples exhibit rigid patterns of negativity whereas couples who report being maritally satisfied show minimal rigidity in the opposite direction \u2014 positivity. This was, and is, a theoretical dilemma: how can clinicians understand and eventually modify distressed relationships when the behavior of satisfied couples are less patterned, less predictable and more diverse? A recent study by Griffin and Li (2015), using contemporary machine learning techniques, reanalyzed existing marital interaction data and found that, contrary to expectation and existing theory, nondistressed couples should be further subdivided into two groups \u2014 those who are predictably positive or neutral and those who interact using diverse and varying levels of positive and negative behaviors. The latter group is the focus of this thesis. Using these recent findings as discussion points, I review how the unexpected behaviors in this novel group can maintain and possibly perpetuate marital satisfaction.
ContributorsAbdul-Quadir, Hasana (Author) / Griffin, William A. (Thesis director) / Li, Xun (Committee member) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor) / School of International Letters and Cultures (Contributor) / Hugh Downs School of Human Communication (Contributor)
Created2015-05
Description
Vulnerability research is a fairly new field of study and has yet to be applied to fields such as improvisation or online content creation. Making vulnerability public in a way that necessitates improvisation is fundamental to YouTube content creation. This Creative Project focuses on drawing connections between Vulnerability and Improvisation as it relates to creating content for YouTube. I did this through creating my own content which consisted of four YouTube videos centering around the theme of embracing fear. I found that in order to create content, I had to practice vulnerability myself and embrace improvisation if I wished to communicate those same themes to my audience. This project revealed that there are many ways which this powerful vulnerability research is yet to be applied, specifically in the realm of vastly public arenas such as YouTube. This research shows that vulnerability is not only applicable to interpersonal relationships, but has the potential to influenced thousands when used in public spheres.
ContributorsEckert, Rebecca Hope (Author) / Branch, Boyd (Thesis director) / Hughes, Erika (Committee member) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor) / School of Film, Dance and Theatre (Contributor) / Department of English (Contributor)
Created2015-05