Filtering by
- Member of: ASU Regents' Professors Open Access Works
![130319-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-04/130319-Thumbnail%20Image.png?versionId=vGJCgNNle7WqEw_Y.qHeSBSG9Zi2UIo9&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240530/us-west-2/s3/aws4_request&X-Amz-Date=20240530T153726Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=eaaa8d753fbf71d15e04639d798ecf41b58f5bd0ac8cca3e8a70877e439fe5ea&itok=hfN9x8Il)
Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.
![130321-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-04/130321-Thumbnail%20Image.png?versionId=Qz8AVLilG_wPQvC4pSqXEh9tMCf4_cfX&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240615/us-west-2/s3/aws4_request&X-Amz-Date=20240615T011115Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=61ca77ee4cdd66df5ea8d38d91f0022d2278ccc3fbb29d6b1121299ff3bf1b21&itok=DBBpN6q4)
![130322-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-04/130322-Thumbnail%20Image.png?versionId=YCHSKaVcygXd3HHenvL2xzS.G.kixC32&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240530/us-west-2/s3/aws4_request&X-Amz-Date=20240530T153726Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=84121a565f274f48d6dad85b0da158b4de106a8b80e67f52cd88a28592f99586&itok=I8O649eW)
Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 μm diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 Ångström were recorded. The diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.
![130433-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-04/130433-Thumbnail%20Image.png?versionId=5FWlNNKutwsGP2wwp2o9_1HyGN7huODW&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240618/us-west-2/s3/aws4_request&X-Amz-Date=20240618T135758Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=6a59492c60824dd76025aa559bb87a12cd0160db7099353c41961ffe2b0d35b0&itok=VLnJC-x4)
![130274-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-04/130274-Thumbnail%20Image.png?versionId=y2otTFmNxVfGdNlboOFY5tZyqTmTOPne&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240618/us-west-2/s3/aws4_request&X-Amz-Date=20240618T134302Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=8d6b60e768cfa8da9ac6138c9d2ccc3e8d3be65f9b7894c5e66ebfd2e0737b3b&itok=3GiCMrZE)
![130284-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-04/130284-Thumbnail%20Image.png?versionId=RL18_PeDsi0QZ7ifKxgMiJEVL3i4mc.x&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240606/us-west-2/s3/aws4_request&X-Amz-Date=20240606T054759Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=f3d6b15d8c73c0cf7d6967a573a9bcefad0d52398c294984b06db431689cb0d5&itok=MkSjOHVT)
![130301-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-04/130301-Thumbnail%20Image.png?versionId=xqj1fyeOr9I9fWJmok_xApUZSi.vLJWr&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240618/us-west-2/s3/aws4_request&X-Amz-Date=20240618T012352Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=09846605a3e524afb4977a655c57fe81fb5854b12d7f3f89f3c5e18aadb98195&itok=_EM0ee44)
![130302-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-04/130302-Thumbnail%20Image.png?versionId=bRVeSoMNtjtOc8xFDxSUq67rlfgCYFH1&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240605/us-west-2/s3/aws4_request&X-Amz-Date=20240605T205427Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=5397aef26823e23c5d1b8a9b8546cb83c5d889ac489d568fdbe3d29d6b0d301f&itok=yeSP2o7w)
![130303-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-04/130303-Thumbnail%20Image.png?versionId=49yWpTidtPKMv58NC2TOxiCt7hU1PUbz&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240618/us-west-2/s3/aws4_request&X-Amz-Date=20240618T054707Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=642ddbb90ebaf5518b51b086896ba7106b6fb63dddd6ccc4e6726f03a17f23fd&itok=8-_QFPiz)
![130308-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-04/130308-Thumbnail%20Image.png?versionId=hbSopyZGn.0PfhCpwXksN.yga5M5e2sd&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240618/us-west-2/s3/aws4_request&X-Amz-Date=20240618T195222Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=76f78356351921d7d32b5feb72251d61aa25e3ff8d1c5e60e828d644e7112e46&itok=tyCc582q)