Matching Items (69)
Description
Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP–SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP–SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.
ContributorsFromme, Raimund (Author) / Ishchenko, Andrii (Author) / Metz, Markus (Author) / Roy Chowdhury, Shatabdi (Author) / Basu, Shibom (Author) / Boutet, Sebastien (Author) / Fromme, Petra (Author) / White, Thomas A. (Author) / Barty, Anton (Author) / Spence, John (Author) / Weierstall, Uwe (Author) / Liu, Wei (Author) / Cherezov, Vadim (Author) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / College of Liberal Arts and Sciences (Contributor) / Department of Physics (Contributor)
Created2015-08-04
Description
Serial femtosecond crystallography (SFX) has opened a new era in crystallography by permitting nearly damage-free, room-temperature structure determination of challenging proteins such as membrane proteins. In SFX, femtosecond X-ray free-electron laser pulses produce diffraction snapshots from nanocrystals and microcrystals delivered in a liquid jet, which leads to high protein consumption. A slow-moving stream of agarose has been developed as a new crystal delivery medium for SFX. It has low background scattering, is compatible with both soluble and membrane proteins, and can deliver the protein crystals at a wide range of temperatures down to 4°C. Using this crystal-laden agarose stream, the structure of a multi-subunit complex, phycocyanin, was solved to 2.5 Å resolution using 300 µg of microcrystals embedded into the agarose medium post-crystallization. The agarose delivery method reduces protein consumption by at least 100-fold and has the potential to be used for a diverse population of proteins, including membrane protein complexes.
ContributorsConrad, Chelsie (Author) / Basu, Shibom (Author) / James, Daniel (Author) / Wang, Dingjie (Author) / Schaffer, Alexander (Author) / Roy Chowdhury, Shatabdi (Author) / Zatsepin, Nadia (Author) / Aquila, Andrew (Author) / Coe, Jesse (Author) / Gati, Cornelius (Author) / Hunter, Mark S. (Author) / Koglin, Jason E. (Author) / Kupitz, Christopher (Author) / Nelson, Garrett (Author) / Subramanian, Ganesh (Author) / White, Thomas A. (Author) / Zhao, Yun (Author) / Zook, James (Author) / Boutet, Sebastien (Author) / Cherezov, Vadim (Author) / Spence, John (Author) / Fromme, Raimund (Author) / Weierstall, Uwe (Author) / Fromme, Petra (Author) / Department of Chemistry and Biochemistry (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / College of Liberal Arts and Sciences (Contributor) / Department of Physics (Contributor) / School of Molecular Sciences (Contributor)
Created2015-06-30
Description
X-ray free-electron lasers (XFELs) provide new opportunities for structure determination of biomolecules, viruses and nanomaterials. With unprecedented peak brilliance and ultra-short pulse duration, XFELs can tolerate higher X-ray doses by exploiting the femtosecond-scale exposure time, and can thus go beyond the resolution limits achieved with conventional X-ray diffraction imaging techniques. Using XFELs, it is possible to collect scattering information from single particles at high resolution, however particle heterogeneity and unknown orientations complicate data merging in three-dimensional space. Using the Linac Coherent Light Source (LCLS), synthetic inorganic nanocrystals with a core–shell architecture were used as a model system for proof-of-principle coherent diffractive single-particle imaging experiments. To deal with the heterogeneity of the core–shell particles, new computational methods have been developed to extract the particle size and orientation from the scattering data to assist data merging. The size distribution agrees with that obtained by electron microscopy and the merged data support a model with a core–shell architecture.
ContributorsLi, Xuanxuan (Author) / Spence, John (Author) / Hogue, Brenda (Author) / Liu, Haiguang (Author) / College of Liberal Arts and Sciences (Contributor) / Department of Physics (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor)
Created2017-08-27
Description
Waiting for a Passenger / Ship to Go to Sea is a performance made by In Kyung Lee and performed by five dancers. It premiered in Americas Gallery at ASU Art Museum on January 20, January 24, and Jan 27, 2015. The work existed in a container of geometric spatial structure and cyclical rhythmic cycles, which were filled with repetition, accumulation, and minimalistic durational movement vocabulary. The dancers courageously ventured through the rigorous and exacting structure, transforming individual and collective struggles and vulnerabilities into the beauty of being human. This document looks into the background and creation process of the work.
ContributorsYi, In-gyŏng (Author) / Standley, Eileen (Thesis advisor) / Ellsworth, Angela (Committee member) / Fitzgerald, Mary (Committee member) / Arizona State University (Publisher)
Created2015
Description
The ASU School of Dance presents School of Dance LIVE!, September 7-9, with works by dance faculty, performed at Galvin Playhouse.
ContributorsPinholster, Jacob (Director) / Koch, Carolyn (Artistic director, Production manager, Lighting designer) / Swayze, William (Musician, Performer, Composer) / Jones, Ben (Performer) / Mack, Austen (Performer) / Ortego, Garrett (Performer) / Benard, Jacqueline (Costume designer, Creator) / Burk, Ashley (Collaborator deprecated, use Contributor, Performer) / Arredondo, Julia (Performer) / Groom, Léla (Performer) / Giordano, Erin (Performer) / Bouey, Billie-Joe "J." (Performer) / Bartholomew, Jessica (Performer) / Chapman, Eric (Collaborator deprecated, use Contributor)) / Peterson, Haley (Collaborator deprecated, use Contributor)) / Vissicaro, Pegge (Collaborator deprecated, use Contributor)) / Mitchell, John D. (Designer, Musician) / Standley, Eileen (Designer, Choreographer, Performer) / Kyriakides, Yannis (Composer) / Tomooka, Kayla (Performer) / Waitz, Jessica (Performer) / McNutt, Eden (Costume designer, Performer) / DeWitt, Inertia (Musician, Performer) / Murphey, Claudia (Director, Interviewer) / Mumford, Jessica (Videographer, Editor) / Britt, Melissa (Choreographer, Costume designer, Performer) / Alvarez, Emily (Musician) / Salcido, Alejandro (Lighting designer) / Calleros, Vince (Performer) / Dimmick, Saza (Performer) / Granado, Michaela (Performer) / Kusch, Liz (Performer) / Lopez, Cassidy (Performer) / Kaplan, Rob (Composer, Performer) / Rex, Melissa S. (Choreographer, Lighting designer, Technical director) / Bocchino, Corinne (Performer, Performer) / Mihaleva, Galina (Costume designer, Creator) / Cooper, Carol (Performer) / Crissman, Angel (Performer) / Bouey, J (Performer) / Bouey, Majee (Performer) / Bouey, Najee (Performer) / Matthews, Emily (Performer) / Vago, Haley (Performer) / Witzke, Nikki (Performer) / Goodson, Naomi (Performer) / Levin, Felicia (Performer) / Gonzales, Anthony (Performer) / Hughs, Haylee (Performer) / Ling, Amanda (Performer) / Pourzal, Kristopher K. Q. (Performer) / Munoz, Jessica (Performer) / Peterson, Britta (Performer) / Poto, Ana Maria (Performer) / Rickert, Austin (Performer) / Schupp, Karen (Director, Costume designer, Performer) / McMahon Ward, Frances (Editor) / Khoilian, Jarek (Cinematographer) / Heath, Jason (Musician) / Page, Martin (Composer) / Fairweather, Brian (Composer) / Thornton, Trevor (Composer) / Richardson, Chris (Composer) / Fitzgerald, Mary (Choreographer, Performer) / Ford, Lindsey (Performer) / Garibay, Elissa (Performer) / Barrett, Kristen (Performer) / Herberger Institute School of Dance (Musician)
Created2012
Description
The ASU School of Dance presents Emerging Artists I, February 9-12, with works by guest artists, dance faculty, and students, performed at Dance Studio Theatre, PEBE 132.
ContributorsSedlack, Kate (Choreographer, Lighting designer, Costume designer) / Bormann, Megan (Dancer) / Brack, Ashley (Dancer) / Hauk, Chelsey (Dancer) / Koeppel, Alex (Dancer) / Pesque, Julia (Dancer) / Roberts, Cassie (Dancer) / Roethler, Eva (Dancer) / Spenceley, Jenni (Dancer) / Trujillo, David (Dancer) / Fitzgerald, Mary (Artistic director, Choreographer, Lighting designer, Costume designer) / Ammerman, Mark C. (Technical director, Set designer, Lighting designer) / Benard, Jacqueline (Costume designer) / Mihaleva, Galina (Costume designer) / Swayze, William (Sound designer, Composer) / Archambault, Jared (Stage manager) / King, Natalie (Dancer) / Skrentny, Adrian (Choreographer, Lighting designer, Costume designer, Set designer, Dancer) / Bajromovic, Saban (Musician) / Parsons, Niamh (Musician) / Cortez, Sarah (Dancer) / Galindo, Rolanda (Dancer) / Kenworthy, Julianna (Dancer, Choreographer, Costume designer) / Kriston, Jordan (Dancer) / Wong, Jennifer (Dancer) / Taylor, Michelle (Choreographer) / Hart, Keira (Lighting designer) / Smith, Vanessa (Costume designer, Dancer) / Chesley, Andrea (Choreographer, Lighting designer, Costume designer, Dancer) / Chappman, Richard (Composer, Performer) / Childers, Emily (Dancer) / Cortez, Maggie (Dancer) / Howe, Martha E. (Choreographer) / Blake, Casey (Lighting designer) / Blukis, Audrey (Dancer) / Holcman, Sarah (Choreographer, Lighting designer, Costume designer, Set designer) / Lecuona, Margarita (Musician) / Santaolalla, Gustavo (Musician) / Aste, Cuti (Musician) / Lobos, Jorge (Musician) / Zamora, Maria Esther (Musician) / González, Polito (Musician) / Lindl, Roberto (Musician) / Sternke, Amy (Dancer) / Anderson, Laurie (Musician) / McGloin, Aaron (Lighting designer) / Bingle, Meghan (Dancer) / Jordan, Paul (Dancer) / Kuo, Alexis I-Cheng (Dancer) / Lederman, Christiana (Dancer) / Lerner, Kristin (Dancer) / Parish, Sara (Dancer) / Hatzis, Amy E. (Choreographer, Composer, Costume designer, Lighting designer) / Dumont, Marguerite (Dancer) / Ebling, Brett (Dancer) / Kettner, Lauren (Dancer) / Pannier, Nicci (Dancer) / Kelley, Jean (Costume designer, Dancer) / Monk, Meredith (Composer) / Kriston, Micah (Choreographer, Lighting designer, Costume designer) / Fox, Stacey (Cinematographer) / Herberger Institute School of Dance (Musician)
Created2006
Description
The ASU School of Dance presents Emerging Artists I, February 15-18, with works by guest artists, dance faculty, and undergrad students, performed at Dance Studio Theatre, PEBE 132.
ContributorsTsukayama, Jennifer (Choreographer) / Benard, Jacqueline (Costume designer) / Dessingue, Colette (Costume designer, Dancer) / Ammerman, Mark (Lighting designer, Set designer) / Burnett, Cherie (Dancer, Choreographer, Costume designer, Lighting designer) / Doherty, Kelly (Dancer) / Lathrop, Cerrin (Dancer, Choreographer, Costume designer, Lighting designer) / Scott, Jillian (Dancer, Choreographer, Costume designer, Lighting designer) / Happel, Nancy (Choreographer, Costume designer) / Brahem, Anauar (Musician) / Kuo, I-Wen (Costume designer) / McGloin, Aaron (Lighting designer, Stage manager) / Kuo, Alexis I-Cheng (Dancer) / Fitzgerald, Mary (Choreographer) / Albeniz, Isaac (Musician) / Nitz, Ryan (Performer) / Mihaleva, Galina (Costume designer) / Vessey, Julia (Lighting designer, Set designer) / Wajih, Sonja (Dancer) / Scott, Laurie (Lighting designer) / Faciana, Maria (Dancer) / Roberts, Cassandra (Dancer) / Millford, Tobie (Musician) / Massiah, Monique (Dancer) / Roberts, Cassie (Dancer) / Van Buskirk, Kelly (Dancer) / Allred, Candice (Choreographer, Costume designer, Lighting designer) / Adams, Shardai (Dancer) / Caron, Bridgette (Dancer) / Hammer, Michelle (Dancer) / Hughes, Whitney (Dancer) / Kozulla, Ashle (Dancer) / Blukis, Audrey (Dancer) / Mc Caskill, Katie (Dancer) / Mooney, Elina (Artistic director) / Koch, Carolyn (Production manager) / Ammerman, Mark C. (Technical director, Set designer) / Swayze, William (Sound designer) / Murphey, Claudia (Artistic director)
Created2007
Description
The ASU School of Dance presents Emerging Artists II, February 16-19, with works by dance faculty and undergrad students, performed at Dance Studio Theatre, PEBE 132.
ContributorsBingle, Meghan (Choreographer, Lighting designer, Costume designer, Dancer) / Anderson, Laurie (Composer) / Callandine, Tess (Dancer) / Fellenz, Aaron (Dancer) / Harrison, Lauren (Dancer) / Jordan, Paul (Dancer) / Lederman, Christiana (Dancer) / Richardson, Kathryn (Dancer, Costume designer) / Smith, Vanessa (Dancer, Dancer) / Tonding, Tara (Dancer, Dancer, Costume designer) / McMahan, Jeff (Choreographer, Director) / Reker, Steven (Costume designer, Dancer) / Kemmerer, Shelley (Choreographer, Musician, Lighting designer, Costume designer, Set designer, Dancer) / Ammerman, Mark C. (Set designer, Lighting designer, Technical director) / Harrison, Christina (Dancer) / Hart, Keira (Lighting designer, Dancer, Costume designer) / Maywald, Marilyn (Dancer, Choreographer, Lighting designer, Costume designer) / Young, Liz (Dancer) / Martinez, Meredith (Choreographer, Lighting designer, Costume designer, Dancer) / Martinez, Cristobal (Composer) / Allred, Candice (Dancer) / Robinett, Danae (Dancer) / Ross, Janie (Dancer) / Hall Mckee, Lisa (Choreographer) / Atwood, Laura (Lighting designer) / Lerner, Kristin (Choreographer, Performer, Lighting designer, Costume designer) / Krauss, Alison (Musician) / Harris, Emmylou (Musician) / Welch, Gillian (Musician) / Goldstein, Malcolm (Musician) / Kenworthy, Julianna (Dancer) / Kriston, Jordan (Dancer) / Sternke, Amy (Dancer) / Greer, Lindsey S. (Choreographer, Composer, Lighting designer, Costume designer) / Candler, Martha (Dancer) / Ebling, Brett (Dancer) / Fox, Megan (Dancer) / Hatzis, Amy E. (Dancer) / Mollicone, Karissa (Dancer) / Van Buskirk, Kelly (Dancer) / Hookala, Rayn (Choreographer, Costume designer, Set designer) / Kukes, Sarah (Lighting designer) / Mihaleva, Galina (Costume designer) / Carlsen, Christine (Videographer) / Schupp, Karen (Choreographer) / Addy, Obo (Composer) / Stevens, Sufjan (Musician) / McGloin, Aaron (Dancer) / Fitzgerald, Mary (Artistic director) / Benard, Jacqueline (Costume designer) / Swayze, William (Sound designer) / Vessey, Julia (Stage manager)
Created2006
Description
The ASU School of Dance presents Emerging Artists II, February 14-17, with works by dance BA and MFA students Cassie Roberts, Katie McCaskill, Lydia Sakolsky-Basquill, Kelly Doherty, and Kristin Tovson, performed at the Dance Studio Theatre.
ContributorsHergerber Institute School of Dance (Publisher) / Ackermann, Jennifer Keane (Choreographer) / McGloin, Aaron (Lighting designer) / Mihaleva, Galina (Costume designer, Costume designer) / Roberts, Cassie (Costume designer, Dancer) / Ebling, Brett (Choreographer, Costume designer) / Trujillo, Davey (Lighting designer, Dancer) / Abruzzi, Lucio (Dancer) / Dumont, Marguerite (Dancer) / Hosack, Brittany (Dancer) / Hauk, Chelsey (Dancer) / Kozulla, Ashle (Dancer) / Murray, Leigh (Dancer) / Mollicone, Karissa (Dancer) / Buskirk, Kelly Van (Dancer) / Fitzgerald, Mary (Choreographer, Costume designer, Set designer) / Koch, Carolyn (Lighting designer) / McCaskill, Katie (Costume designer, Dancer) / Ammerman, Mark (Set designer) / Limon, Jose (Choreographer) / Sakolsky-Basquill, Lydia (Costume designer, Dancer) / Doherty, Kelley (Choreographer, Costume designer, Dancer) / Benard, Jacqueline (Costume designer) / Faciana, Maria (Dancer)
Created2008
Description
The ASU School of Dance presents Emerging Artists I, October 24-26, with works by dance MFA candidates Sara Malan-McDonald and Holly Woodridge, performed at the Dance Studio Theatre, PEBE 132.
ContributorsHerberger Institute School of Dance (Publisher) / Malan-McDonald, Sara (Choreographer, Costume designer, Dancer) / Millicam, Kendahl Lyn (Collaborator deprecated, use Contributor, Dancer) / Woolridge, Holly (Choreographer, Costume designer) / Barrett, Rachael (Dancer) / Boles, Leah (Dancer) / Claudel, Kacie (Dancer) / Harding, Susannah (Dancer) / Horton, Toni (Dancer) / Kusch, Liz (Dancer) / Medina, Tasheena (Dancer) / Sadler, Jessica (Dancer) / Koch, Carolyn (Lighting designer) / Benard, Jacqueline (Costume designer) / Mapes, Aileen (Costume designer, Dancer) / Mihaleva, Galina (Costume designer) / Ammerman, Mark C. (Set designer) / Beck, Jeanette (Dancer) / Gonzalez, Elissa (Dancer) / Hook, Brittany (Dancer) / Kroon, Anjuli (Dancer) / Landon, Melissa (Dancer) / Levin, Gianna (Dancer) / Levin, Gillian (Dancer) / Breen, Leslie (Collaborator deprecated, use Contributor)) / Feliciano, Cynthia (Collaborator deprecated, use Contributor)) / Fitzgerald, Mary (Choreographer, Costume designer) / Basting, Samantha (Dancer) / DeVries, Debbie (Dancer) / McDonald, Aaron (Dancer) / Tovson, Kristin (Dancer) / Endicott, Jenna (Dancer) / Fisher, Rose (Dancer) / Kirwan, Molly (Dancer)
Created2008