Matching Items (33)
Description
Serial femtosecond crystallography (SFX) has opened a new era in crystallography by permitting nearly damage-free, room-temperature structure determination of challenging proteins such as membrane proteins. In SFX, femtosecond X-ray free-electron laser pulses produce diffraction snapshots from nanocrystals and microcrystals delivered in a liquid jet, which leads to high protein consumption. A slow-moving stream of agarose has been developed as a new crystal delivery medium for SFX. It has low background scattering, is compatible with both soluble and membrane proteins, and can deliver the protein crystals at a wide range of temperatures down to 4°C. Using this crystal-laden agarose stream, the structure of a multi-subunit complex, phycocyanin, was solved to 2.5 Å resolution using 300 µg of microcrystals embedded into the agarose medium post-crystallization. The agarose delivery method reduces protein consumption by at least 100-fold and has the potential to be used for a diverse population of proteins, including membrane protein complexes.
ContributorsConrad, Chelsie (Author) / Basu, Shibom (Author) / James, Daniel (Author) / Wang, Dingjie (Author) / Schaffer, Alexander (Author) / Roy Chowdhury, Shatabdi (Author) / Zatsepin, Nadia (Author) / Aquila, Andrew (Author) / Coe, Jesse (Author) / Gati, Cornelius (Author) / Hunter, Mark S. (Author) / Koglin, Jason E. (Author) / Kupitz, Christopher (Author) / Nelson, Garrett (Author) / Subramanian, Ganesh (Author) / White, Thomas A. (Author) / Zhao, Yun (Author) / Zook, James (Author) / Boutet, Sebastien (Author) / Cherezov, Vadim (Author) / Spence, John (Author) / Fromme, Raimund (Author) / Weierstall, Uwe (Author) / Fromme, Petra (Author) / Department of Chemistry and Biochemistry (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / College of Liberal Arts and Sciences (Contributor) / Department of Physics (Contributor) / School of Molecular Sciences (Contributor)
Created2015-06-30
Description
X-ray free-electron lasers (XFELs) provide new opportunities for structure determination of biomolecules, viruses and nanomaterials. With unprecedented peak brilliance and ultra-short pulse duration, XFELs can tolerate higher X-ray doses by exploiting the femtosecond-scale exposure time, and can thus go beyond the resolution limits achieved with conventional X-ray diffraction imaging techniques. Using XFELs, it is possible to collect scattering information from single particles at high resolution, however particle heterogeneity and unknown orientations complicate data merging in three-dimensional space. Using the Linac Coherent Light Source (LCLS), synthetic inorganic nanocrystals with a core–shell architecture were used as a model system for proof-of-principle coherent diffractive single-particle imaging experiments. To deal with the heterogeneity of the core–shell particles, new computational methods have been developed to extract the particle size and orientation from the scattering data to assist data merging. The size distribution agrees with that obtained by electron microscopy and the merged data support a model with a core–shell architecture.
ContributorsLi, Xuanxuan (Author) / Spence, John (Author) / Hogue, Brenda (Author) / Liu, Haiguang (Author) / College of Liberal Arts and Sciences (Contributor) / Department of Physics (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor)
Created2017-08-27
Description
Structural studies on living cells by conventional methods are limited to low resolution because radiation damage kills cells long before the necessary dose for high resolution can be delivered. X-ray free-electron lasers circumvent this problem by outrunning key damage processes with an ultra-short and extremely bright coherent X-ray pulse. Diffraction-before-destruction experiments provide high-resolution data from cells that are alive when the femtosecond X-ray pulse traverses the sample. This paper presents two data sets from micron-sized cyanobacteria obtained at the Linac Coherent Light Source, containing a total of 199,000 diffraction patterns. Utilizing this type of diffraction data will require the development of new analysis methods and algorithms for studying structure and structural variability in large populations of cells and to create abstract models. Such studies will allow us to understand living cells and populations of cells in new ways. New X-ray lasers, like the European XFEL, will produce billions of pulses per day, and could open new areas in structural sciences.
Contributorsvan der Schot, Gijs (Author) / Svenda, Martin (Author) / Maia, Filipe R. N. C. (Author) / Hantke, Max F. (Author) / DePonte, Daniel P. (Author) / Seibert, M. Marvin (Author) / Aquila, Andrew (Author) / Schulz, Joachim (Author) / Kirian, Richard (Author) / Liang, Mengning (Author) / Stellato, Francesco (Author) / Bari, Sadia (Author) / Iwan, Bianca (Author) / Andreasson, Jakob (Author) / Timneanu, Nicusor (Author) / Bielecki, Johan (Author) / Westphal, Daniel (Author) / Nunes de Almeida, Francisca (Author) / Odic, Dusko (Author) / Hasse, Dirk (Author) / Carlsson, Gunilla H. (Author) / Larsson, Daniel S. D. (Author) / Barty, Anton (Author) / Martin, Andrew V. (Author) / Schorb, Sebastian (Author) / Bostedt, Christoph (Author) / Bozek, John D. (Author) / Carron, Sebastian (Author) / Ferguson, Ken (Author) / Rolles, Daniel (Author) / Rudenko, Artem (Author) / Epp, Sascha W. (Author) / Foucar, Lutz (Author) / Rudek, Benedikt (Author) / Erk, Benjamin (Author) / Hartmann, Robert (Author) / Kimmel, Nils (Author) / Holl, Peter (Author) / Englert, Lars (Author) / Loh, N. Duane (Author) / Chapman, Henry N. (Author) / Andersson, Inger (Author) / Hajdu, Janos (Author) / Ekeberg, Tomas (Author) / College of Liberal Arts and Sciences (Contributor)
Created2016-08-01
Description
The ASU School of Dance presents Undergraduate Projects Showing, October 25-26, with works by undergraduate dance students, performed at Margaret Gisolo Dance Studio.
ContributorsPinholster, Jacob (Director, Artistic director) / Koch, Carolyn (Production manager) / Rex, Melissa S. (Technical director, Lighting designer) / Swayze, William (Musician) / Benard, Jacqueline (Costume designer) / Mihaleva, Galina (Costume designer) / Dodt, Alli (Artistic director) / Edwards, Allison (Artistic director, Performer) / Levin, Felicia (Performer) / Hughes, Haylee (Performer) / Groom, Léla (Performer) / Gastelo, Jr., Gabriel (Lighting designer) / Singleton, Kiah (Choreographer, Lighting designer, Costume designer, Performer) / DeSantis, Kimberly (Performer) / Freirich, Gordon (Performer) / Harkey, Noah (Performer) / Nguyen, Dana (Performer) / Bartholomew, Jessica (Choreographer, Musician, Costume designer) / Dorrel, Kayla (Performer) / Johnson, Sarah (Performer) / LeBlanc, Casey (Performer) / Norris, Sarah (Performer) / Rivera, Paola (Performer) / Sammons, Rylee (Performer) / Soto, Jose (Performer) / Wardarski, Jessie (Performer) / Witzke, Nikki (Performer, Choreographer, Costume designer) / Moraco, Steve (Videographer, Videographer) / Jackson, Sydney (Choreographer, Costume designer) / Castronova, Naomi (Performer) / Garcia, Lacee (Performer) / Keefe, Shelby (Performer) / Kerr, Elena (Performer) / Waitz, Jessica (Performer) / Riojas, David (Choreographer, Costume designer) / Calvano, Jourdan (Performer) / Locker, Rain (Performer) / Pullman, Gracie (Performer) / Reis, Ashley (Performer) / Nunn, Jasmine (Choreographer, Costume designer) / Koji, Saito (Musician) / Avery, Vickie (Musician) / Salcido, Alejandro (Lighting designer) / Baker, Ashley (Performer) / Siegfried, Jordyn (Performer) / Yoder, Allyson (Performer) / Gallagher, Grace (Choreographer, Costume designer, Videographer, Performer) / Dix, Geoffrey (Musician) / Stephens, Alexis (Choreographer, Costume designer, Performer) / Keating, Zoe (Musician) / Hernandez, Martha "Patty" (Choreographer, Costume designer, Performer) / Yuen, Priscilla (Performer) / Herberger Institute School of Dance (Musician)
Created2012
Description
The ASU School of Dance presents School of Dance LIVE!, September 7-9, with works by dance faculty, performed at Galvin Playhouse.
ContributorsPinholster, Jacob (Director) / Koch, Carolyn (Artistic director, Production manager, Lighting designer) / Swayze, William (Musician, Performer, Composer) / Jones, Ben (Performer) / Mack, Austen (Performer) / Ortego, Garrett (Performer) / Benard, Jacqueline (Costume designer, Creator) / Burk, Ashley (Collaborator deprecated, use Contributor, Performer) / Arredondo, Julia (Performer) / Groom, Léla (Performer) / Giordano, Erin (Performer) / Bouey, Billie-Joe "J." (Performer) / Bartholomew, Jessica (Performer) / Chapman, Eric (Collaborator deprecated, use Contributor)) / Peterson, Haley (Collaborator deprecated, use Contributor)) / Vissicaro, Pegge (Collaborator deprecated, use Contributor)) / Mitchell, John D. (Designer, Musician) / Standley, Eileen (Designer, Choreographer, Performer) / Kyriakides, Yannis (Composer) / Tomooka, Kayla (Performer) / Waitz, Jessica (Performer) / McNutt, Eden (Costume designer, Performer) / DeWitt, Inertia (Musician, Performer) / Murphey, Claudia (Director, Interviewer) / Mumford, Jessica (Videographer, Editor) / Britt, Melissa (Choreographer, Costume designer, Performer) / Alvarez, Emily (Musician) / Salcido, Alejandro (Lighting designer) / Calleros, Vince (Performer) / Dimmick, Saza (Performer) / Granado, Michaela (Performer) / Kusch, Liz (Performer) / Lopez, Cassidy (Performer) / Kaplan, Rob (Composer, Performer) / Rex, Melissa S. (Choreographer, Lighting designer, Technical director) / Bocchino, Corinne (Performer, Performer) / Mihaleva, Galina (Costume designer, Creator) / Cooper, Carol (Performer) / Crissman, Angel (Performer) / Bouey, J (Performer) / Bouey, Majee (Performer) / Bouey, Najee (Performer) / Matthews, Emily (Performer) / Vago, Haley (Performer) / Witzke, Nikki (Performer) / Goodson, Naomi (Performer) / Levin, Felicia (Performer) / Gonzales, Anthony (Performer) / Hughs, Haylee (Performer) / Ling, Amanda (Performer) / Pourzal, Kristopher K. Q. (Performer) / Munoz, Jessica (Performer) / Peterson, Britta (Performer) / Poto, Ana Maria (Performer) / Rickert, Austin (Performer) / Schupp, Karen (Director, Costume designer, Performer) / McMahon Ward, Frances (Editor) / Khoilian, Jarek (Cinematographer) / Heath, Jason (Musician) / Page, Martin (Composer) / Fairweather, Brian (Composer) / Thornton, Trevor (Composer) / Richardson, Chris (Composer) / Fitzgerald, Mary (Choreographer, Performer) / Ford, Lindsey (Performer) / Garibay, Elissa (Performer) / Barrett, Kristen (Performer) / Herberger Institute School of Dance (Musician)
Created2012
Description
The ASU School of Dance presents Graduate Projects Showing, April 4-5, with works by dance graduate students, performed at Margaret Gisolo Dance Studio, PEBE 132.
ContributorsPellegrino, Laura (Performer, Costume designer, Set designer) / Ullom, Kathryn (Performer, Costume designer, Set designer, Sound designer, Artistic director) / Benard, Jacqueline (Costume designer) / Akerly, Julie (Choreographer, Musician, Costume designer, Videographer, Performer) / LeBlanc, Casey (Choreographer) / Rivera, Paola (Choreographer) / Salcido, Alejandro (Lighting designer, Performer) / Dorvel, Kayla (Choreographer, Performer) / Groom, Chloe (Choreographer, Performer) / Pietruszka, Emily (Choreographer, Performer) / Finlayson, Kelsey (Choreographer, Costume designer, Videographer, Performer) / Richter, Max (Musician) / Barrett, Kristen (Performer) / Bartholomew, Jessica (Performer) / Edmonds, Hallie (Performer) / Waxlax, Kenze (Performer) / Wall-MacLane, Laurel (Choreographer, Sound designer, Costume designer, Performer) / Kelly, Anthony James (Lighting designer, Performer) / Waugh, Whitney (Choreographer, Costume designer, Performer) / Cheney, Bethany (Choreographer, Costume designer, Performer) / Reich, Steve (Musician) / Nicolai, Carsten (Musician) / Koch, Carolyn (Lighting designer, Production manager) / Muhammah-Hays, Ajah (Performer) / Kerr, Elena (Performer) / Montoya, Reyna (Performer) / Pulliam, Gracie (Performer) / Stein, Denise A. (Choreographer, Composer, Lighting designer, Costume designer, Performer) / Tankian, Serj (Composer) / Malakian, Daron (Composer) / Karlsson, Christian (Composer) / Winnberg, Pontus (Composer) / Wyatt, Andrew (Composer) / DeWitt, Inertia (Conceptor, Videographer, Performer) / Catron, Jade (Performer) / Munoz, Jessica (Performer) / Singleton, Kiah (Performer) / Rex-Flint, Melissa S. (Technical director) / Pourzal, Kristopher K. Q. (Choreographer, Costume designer, Performer) / Rombi, Philippe (Composer) / Dessay, Natalie (Musician) / Crissman, Angel (Choreographer, Musician, Lighting designer, Costume designer, Performer) / Ragsdale, Tricia (Choreographer) / Ray, Johnny (Musician) / Reis, Ashley (Performer) / Stephens, Alexis (Performer) / Redondo, Steven (Performer) / Wardarski, Jessie (Performer) / Veigh, Joshua (Performer) / Peterson, Britta Joy (Choreographer, Lighting designer, Costume designer) / Edwards, Allison (Performer) / Gastelo, Gabriel (Performer) / Goodson, Naomi (Performer) / Groom, Léla (Performer) / Levin, Felicia (Performer) / Rodriguez, Juan (Performer) / Dove, Simon (Artistic director)
Created2012
Description
The ASU School of Dance presents The Dance Annual, March 2-4, with works by dance faculty, alumni, graduate, undergraduate, and visiting artists, performed at the Galvin Playhouse Theatre.
ContributorsGonzales, Anthony (Choreographer, Lighting designer, Costume designer, Performer) / Law, Cullen Arthur (Musician) / Koch, Carolyn (Lighting designer, Production manager) / Benard, Jacqueline (Costume designer) / Cooper, Hannah (Performer) / Giordano, Erin (Performer) / Goodson, Naomi L. (Performer) / Konzal, Michaela (Performer) / Lopez, Cassidy (Performer) / Pellegrino, Laura (Performer, Choreographer, Film editor, Costume designer, Set designer) / Pietruszka, Emily (Performer) / Rodriguez, Juan (Performer, Choreographer, Lighting designer) / Wall-MacLane, Laurel (Choreographer, Lighting designer, Costume designer) / Chapman, Eric (Performer) / Kelly, Anthony James (Performer) / Montoya, Reyna (Performer) / Munoz, Jessica (Performer) / Nunn, Jasmine (Performer) / Singleton, Kiah (Performer) / Rex, Melissa S. (Lighting designer, Technical director) / Peterson, Britta Joy (Choreographer, Lighting designer, Costume designer) / Vining, Robin (Composer) / Groom, Léla (Costume designer, Performer) / Forest, McKenna (Performer) / Levin, Felicia (Performer) / Sykes, Cleo (Performer) / Bartholomew, Jessica (Performer) / Crissman, Angel (Performer) / Edwards, Allison (Performer) / Gastelo, Gabriel (Performer) / Stein, Denise A. (Performer, Choreographer, Lighting designer, Costume designer) / Gersony, Alyssa (Choreographer, Costume designer, Performer) / Ford, Mary (Lighting designer) / Schupp, Miss Karen (Choreographer) / Page, Martin (Composer) / Fairweather, Brian (Composer) / Thornton, Trevor (Composer) / Richardson, Chris (Composer) / Yellman, Billy (Composer) / Arredondo, Julie (Performer) / Bouey, Billie-Joe "J." (Performer) / Burk, Ashley (Performer) / Matthews, Emily (Performer) / Vago, Haley (Performer) / Witzke, Nikki (Performer) / Standley, Eileen (Choreographer) / Kyriakides, Yannis (Musician) / Moor, Andy (Musician) / Mihaleva, Galina (Costume designer) / Ling, Amanda (Performer) / Handel, George Friederic (Composer) / Catron, Jade (Performer) / Alvarez, Emily (Musician) / Dove, Simon (Artistic director)
Created2012
Description
The ASU School of Dance presents Undergraduate Projects Showing, October 27-28, with works by dance undergraduate students performed at the Dance Studio Theatre, PEBE 132.
*Tape 2 for October 28th is not available due to the original video tape being corrupted.
ContributorsDove, Simon (Director, Artistic director) / Koch, Carolyn (Production manager) / Rex, Melissa S. (Technical director) / Gonzales, Anthony (Choreographer, Lighting designer) / Rodriguez, Juan (Performer) / Bouey, Billie-Joe "J." (Choreographer, Lighting designer, Costume designer) / Burke, Ashley (Performer) / Catron, Jade (Performer) / Karr, Elena (Performer) / Larkin, Cody (Performer) / LeBlanc, Casey (Performer) / Levin, Felicia (Performer) / Lewicki, Luke (Performer) / Munoz, Jessica (Performer) / Norris, Sarah (Performer) / Pietruska, Emily (Performer) / Reis, Ashley (Performer) / Rivera, Paolo (Performer) / Stevens, Alexis (Performer) / Vago, Haley (Performer) / Yuen, Pricilla (Choreographer, Lighting designer, Costume designer, Performer) / Cooper, Hannah (Lighting designer, Artistic director) / Alvarez, Emily (Choreographer, Costume designer, Performer) / Salcido, Alex (Lighting designer)
Created2011
Description
The ASU School of Dance presents Graduate Projects Showing, October 20-21, with works by dance graduate students, performed at Margaret Dance Studio, PEBE 132.
ContributorsPeterson, Britta Joy (Choreographer, Lighting designer, Costume designer) / Rex, Melissa S. (Lighting designer, Technical director) / Groom, Léla (Costume designer, Performer, Performer) / Benard, Jacqueline (Costume designer) / Levin, Felicia (Costume designer, Performer) / Sykes, Cleo (Costume designer, Performer) / Forest, McKenna (Costume designer, Performer) / Bartholomew, Jessica (Performer) / Barrett, Kristen (Performer) / Edwards, Allison (Performer) / Gastelo, Gabriel (Performer) / Rodriguez, Juan (Performer) / Stein, Denise A. (Choreographer, Lighting designer, Costume designer, Performer) / Akerly, Julie (Choreographer) / Salcido, Alejandro (Lighting designer) / Peterson, Haley (Costume designer) / Crissman, Angel (Choreographer, Costume designer, Performer) / Derrick, Jardon (Performer) / Groom, Chloe (Performer) / Pietruszka, Emily (Performer) / Buck, Helen (Videographer) / Finlayson, Kelsey (Choreographer, Costume designer) / Wall-MacLane, Laurel (Choreographer, Lighting designer, Costume designer) / Burk, Ashley (Performer) / Hampton, Samantha (Performer) / Stephans, Alexis (Performer) / Stuard, Jacque (Performer) / Webber, Jessica (Performer) / Ragsdale, Tricia (Choreographer, Costume designer, Performer) / Ramsey, Ashleee Jo (Choreographer, Performer) / Kelly, Anthony James (Lighting designer, Performer) / Pourzal, Kristopher Kareem Quarles (Choreographer, Videographer, Costume designer) / Bradley, LaTefia (Performer) / Chapman, Eric (Performer) / Cooper, Hannah (Performer) / Montoya, Reyna (Performer) / Munoz, Jessica (Performer) / Nunn, Jasmine (Performer) / Singleton, Kiah (Performer) / DeWitt, Inertia (Choreographer, Costume designer, Videographer, Performer) / Ling, Amanda (Choreographer, Costume designer, Performer) / Antonietti, Lilly (Performer) / Dorbel, Kayla (Performer) / Adams, Alexandra (Performer) / Reis, Ashley (Performer) / Rivera, Paola (Performer) / Catron, Jade (Performer)
Created2011
Description
The ASU School of Dance presents The Dance Annual, April 15-17, with works by dance faculty, alumni, graduate, undergraduate, and visiting artists, performed at Margaret Gisolo Dance Studio.
ContributorsPellegrino, Laura (Choreographer, Editor, Costume designer, Performer, Dancer) / Ullom, Kathryn (Costume designer, Performer) / Rodriguez, Juan (Choreographer, Lighting designer, Performer, Costume designer) / Bouey, BJ (Lighting designer) / Benard, Jacqueline (Costume designer) / Mihaleva, Galina (Costume designer) / Burke, Ashley (Performer) / Laney, Echo (Performer) / Levin, Felicia (Performer, Dancer) / Groom, Léla (Performer) / Sykes, Cleo (Performer) / Kelly, Anthony James (Choreographer, Lighting designer, Performer) / Angkham, Veng David (Performer) / Codina, Tessa (Performer) / Cooper, Hannah (Performer) / Konzal, Michaela (Performer) / Schneider, Samantha (Performer) / Hillerby, Rebecca Blair, Penelope Salt (Choreographer, Performer, Dancer) / Bedford, Crystal (Performer, Choreographer, Costume designer, Dancer) / Kosowski, Jenna (Choreographer) / Wrobel, Tara, Georgina Bliss (Lighting designer, Choreographer, Costume designer, Performer, Dancer) / Zuccola, Renee (Performer) / Wall-MacLane, Laurel (Choreographer, Performer) / Chapman, Eric (Performer, Costume designer) / Gonzales, Anthony (Performer, Dancer) / Brown, Alyssa (Performer, Dancer) / Karr, Mandi (Performer, Dancer) / McHale, Samantha (Performer) / Mayes, Paige (Performer, Costume designer) / Myers, III, J. Ivory (Performer) / Tamez, Laura (Performer, Costume designer) / Wilcox, Madeline (Performer) / Mitchell, John (Performer) / Standley, Eileen (Performer) / Page, Monica (Performer) / Yuen, Priscilla (Performer) / Duarte, Cristian (Choreographer) / Koch, Carolyn (Lighting designer) / Ford, Mary (Performer, Costume designer, Dancer) / Gersony, Alyssa (Performer, Costume designer, Dancer) / Millizer, Emily (Performer, Costume designer, Dancer) / Waugh, Whitney, Foxy McGillicutty (Performer, Costume designer, Dancer) / Zakrewski, Emily, Pennelopi Lawson (Performer, Costume designer, Dancer) / Britt, Melissa (Choreographer) / Castro, Angel (Lighting designer) / Lerma, Miguel (Choreographer, Costume designer, Performer) / Spranger, Emily (Choreographer, Costume designer) / Vining, Robin (Composer) / Trujillo, Davey (Lighting designer) / Ammerman, Mark C. (Set designer, Lighting designer) / Fletcher, Kasey (Dancer) / Ferrell, Rebecca A. (Choreographer, Costume designer, Dancer) / Hull, Scott (Sound designer) / Raviotta, Todd A. (Sound designer) / Ling, Amanda, Blair Ellis (Performer, Dancer) / Barnard, Ashley (Dancer) / Bocchino, Corinne (Dancer) / Bouey, J (Dancer) / Montoya, Reyna (Dancer)
Created2011