Matching Items (60)
Description
Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ∼700 Å diameter. Microcrystals delivered in viscous agarose medium diffracted to ∼40 Å resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is an important step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.
ContributorsLawrence, Robert (Author) / Conrad, Chelsie (Author) / Zatsepin, Nadia (Author) / Grant, Thomas D. (Author) / Liu, Haiguang (Author) / James, Daniel (Author) / Nelson, Garrett (Author) / Subramanian, Ganesh (Author) / Aquila, Andrew (Author) / Hunter, Mark S. (Author) / Liang, Mengning (Author) / Boutet, Sebastien (Author) / Coe, Jesse (Author) / Spence, John (Author) / Weierstall, Uwe (Author) / Liu, Wei (Author) / Fromme, Petra (Author) / Cherezov, Vadim (Author) / Hogue, Brenda (Author) / Biodesign Institute (Contributor) / Infectious Diseases and Vaccinology (Contributor) / Applied Structural Discovery (Contributor) / Department of Chemistry and Biochemistry (Contributor) / College of Liberal Arts and Sciences (Contributor) / Department of Physics (Contributor) / School of Life Sciences (Contributor)
Created2015-08-20
Description
Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP–SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP–SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.
ContributorsFromme, Raimund (Author) / Ishchenko, Andrii (Author) / Metz, Markus (Author) / Roy Chowdhury, Shatabdi (Author) / Basu, Shibom (Author) / Boutet, Sebastien (Author) / Fromme, Petra (Author) / White, Thomas A. (Author) / Barty, Anton (Author) / Spence, John (Author) / Weierstall, Uwe (Author) / Liu, Wei (Author) / Cherezov, Vadim (Author) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / College of Liberal Arts and Sciences (Contributor) / Department of Physics (Contributor)
Created2015-08-04
Description
Serial femtosecond crystallography (SFX) has opened a new era in crystallography by permitting nearly damage-free, room-temperature structure determination of challenging proteins such as membrane proteins. In SFX, femtosecond X-ray free-electron laser pulses produce diffraction snapshots from nanocrystals and microcrystals delivered in a liquid jet, which leads to high protein consumption. A slow-moving stream of agarose has been developed as a new crystal delivery medium for SFX. It has low background scattering, is compatible with both soluble and membrane proteins, and can deliver the protein crystals at a wide range of temperatures down to 4°C. Using this crystal-laden agarose stream, the structure of a multi-subunit complex, phycocyanin, was solved to 2.5 Å resolution using 300 µg of microcrystals embedded into the agarose medium post-crystallization. The agarose delivery method reduces protein consumption by at least 100-fold and has the potential to be used for a diverse population of proteins, including membrane protein complexes.
ContributorsConrad, Chelsie (Author) / Basu, Shibom (Author) / James, Daniel (Author) / Wang, Dingjie (Author) / Schaffer, Alexander (Author) / Roy Chowdhury, Shatabdi (Author) / Zatsepin, Nadia (Author) / Aquila, Andrew (Author) / Coe, Jesse (Author) / Gati, Cornelius (Author) / Hunter, Mark S. (Author) / Koglin, Jason E. (Author) / Kupitz, Christopher (Author) / Nelson, Garrett (Author) / Subramanian, Ganesh (Author) / White, Thomas A. (Author) / Zhao, Yun (Author) / Zook, James (Author) / Boutet, Sebastien (Author) / Cherezov, Vadim (Author) / Spence, John (Author) / Fromme, Raimund (Author) / Weierstall, Uwe (Author) / Fromme, Petra (Author) / Department of Chemistry and Biochemistry (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / College of Liberal Arts and Sciences (Contributor) / Department of Physics (Contributor) / School of Molecular Sciences (Contributor)
Created2015-06-30
Description
X-ray free-electron lasers (XFELs) provide new opportunities for structure determination of biomolecules, viruses and nanomaterials. With unprecedented peak brilliance and ultra-short pulse duration, XFELs can tolerate higher X-ray doses by exploiting the femtosecond-scale exposure time, and can thus go beyond the resolution limits achieved with conventional X-ray diffraction imaging techniques. Using XFELs, it is possible to collect scattering information from single particles at high resolution, however particle heterogeneity and unknown orientations complicate data merging in three-dimensional space. Using the Linac Coherent Light Source (LCLS), synthetic inorganic nanocrystals with a core–shell architecture were used as a model system for proof-of-principle coherent diffractive single-particle imaging experiments. To deal with the heterogeneity of the core–shell particles, new computational methods have been developed to extract the particle size and orientation from the scattering data to assist data merging. The size distribution agrees with that obtained by electron microscopy and the merged data support a model with a core–shell architecture.
ContributorsLi, Xuanxuan (Author) / Spence, John (Author) / Hogue, Brenda (Author) / Liu, Haiguang (Author) / College of Liberal Arts and Sciences (Contributor) / Department of Physics (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor)
Created2017-08-27
Description
The ASU School of Dance presents Emerging Artists I, February 9-12, with works by guest artists, dance faculty, and students, performed at Dance Studio Theatre, PEBE 132.
ContributorsSedlack, Kate (Choreographer, Lighting designer, Costume designer) / Bormann, Megan (Dancer) / Brack, Ashley (Dancer) / Hauk, Chelsey (Dancer) / Koeppel, Alex (Dancer) / Pesque, Julia (Dancer) / Roberts, Cassie (Dancer) / Roethler, Eva (Dancer) / Spenceley, Jenni (Dancer) / Trujillo, David (Dancer) / Fitzgerald, Mary (Artistic director, Choreographer, Lighting designer, Costume designer) / Ammerman, Mark C. (Technical director, Set designer, Lighting designer) / Benard, Jacqueline (Costume designer) / Mihaleva, Galina (Costume designer) / Swayze, William (Sound designer, Composer) / Archambault, Jared (Stage manager) / King, Natalie (Dancer) / Skrentny, Adrian (Choreographer, Lighting designer, Costume designer, Set designer, Dancer) / Bajromovic, Saban (Musician) / Parsons, Niamh (Musician) / Cortez, Sarah (Dancer) / Galindo, Rolanda (Dancer) / Kenworthy, Julianna (Dancer, Choreographer, Costume designer) / Kriston, Jordan (Dancer) / Wong, Jennifer (Dancer) / Taylor, Michelle (Choreographer) / Hart, Keira (Lighting designer) / Smith, Vanessa (Costume designer, Dancer) / Chesley, Andrea (Choreographer, Lighting designer, Costume designer, Dancer) / Chappman, Richard (Composer, Performer) / Childers, Emily (Dancer) / Cortez, Maggie (Dancer) / Howe, Martha E. (Choreographer) / Blake, Casey (Lighting designer) / Blukis, Audrey (Dancer) / Holcman, Sarah (Choreographer, Lighting designer, Costume designer, Set designer) / Lecuona, Margarita (Musician) / Santaolalla, Gustavo (Musician) / Aste, Cuti (Musician) / Lobos, Jorge (Musician) / Zamora, Maria Esther (Musician) / González, Polito (Musician) / Lindl, Roberto (Musician) / Sternke, Amy (Dancer) / Anderson, Laurie (Musician) / McGloin, Aaron (Lighting designer) / Bingle, Meghan (Dancer) / Jordan, Paul (Dancer) / Kuo, Alexis I-Cheng (Dancer) / Lederman, Christiana (Dancer) / Lerner, Kristin (Dancer) / Parish, Sara (Dancer) / Hatzis, Amy E. (Choreographer, Composer, Costume designer, Lighting designer) / Dumont, Marguerite (Dancer) / Ebling, Brett (Dancer) / Kettner, Lauren (Dancer) / Pannier, Nicci (Dancer) / Kelley, Jean (Costume designer, Dancer) / Monk, Meredith (Composer) / Kriston, Micah (Choreographer, Lighting designer, Costume designer) / Fox, Stacey (Cinematographer) / Herberger Institute School of Dance (Musician)
Created2006
Description
The ASU School of Dance presents Rhythms of Life, November 3-5, with works by guest artists performed at Dance Studio Theatre, PEBE 132.
ContributorsVissicaro, Pegge (Artistic director) / Mooney, Elina (Artistic director) / Koch, Carolyn (Production manager) / Ammerman, Mark C. (Technical director, Lighting designer, Set designer) / Mihaleva, Galina (Costume designer) / Benard, Jacqueline (Costume designer) / Swayze, William (Sound designer) / Valentin-Martinez, Joel (Artistic director, Choreographer) / Amegago, Modesto Mawulolo (Performer) / Chipaumire, Nora (Performer, Choreographer) / Butty, Tamba (Director, Performer) / Bodou, Princess (Performer) / Byne, Princess (Performer) / Dogbeh, Decontee (Performer) / Fredericks, Roseline (Performer) / Gaie, Aaron (Performer) / Morris, Janet (Performer) / Morris, Musa (Performer) / Morris, Musu (Performer) / Morris, Peace (Performer) / Morris, Viddy (Performer) / Nyankum, William (Performer) / Toe, Eliza (Performer) / Wallace, Chuco Glen (Performer) / Caldee, Víctor (Director, Performer) / Cactellanos, Yaisa Pérez (Performer) / Rodíguez, Edwin J (Performer) / Alvarez, Idalberto (Performer) / Becerra, Jhon (Performer) / Domínguez, Miguel (Performer) / Wilson, Sule Greg C. (Choreographer, Performer) / Downs, Lila (Musician) / Milam, Nichole (Dancer) / Verrelli, Emily (Dancer) / Yanez, Avery (Dancer) / Silvestre, Rosangela (Choreographer) / Lunn, Dani (Dancer, Dancer) / Badaró, Luiz (Musician) / Edwards, Margit (Dancer) / Brundage, Kirk (Musician) / Pallais, Mario (Musician) / Moraes, Katia (Vocalist deprecated, use Singer)) / dos Santos, Maria Lourdes Silvestre (Costume designer) / Williams, Shelby (Dancer) / Jones, Erica (Dancer) / Abdullah, Laila (Dancer) / Mullen, Kimberly (Dancer) / Yulin, Linda (Dancer) / Oliveira, Valter (Costume designer)
Created2006
Description
The ASU School of Dance presents Celebrating the Nagrin Legend, September 16-18, with works by dance faculty and visiting artists, performed at the Galvin Playhouse Theatre.
ContributorsNagrin, Daniel (Choreographer) / O'Hara, Shane (Performer) / Mooney, Elina (Choreographer, Dancer, Artistic director) / Cage, John (Musician) / Bolcom, William (Musician) / Ammerman, Mark C. (Set designer, Technical director) / Mihaleva, Galina (Costume designer, Set designer) / Benard, Jacqueline (Costume designer, Set designer) / Waters, Renee (Dancer) / Keuter, Cliff (Choreographer) / Chopin, Frederic (Musician) / Ammerman, Candace (Dancer) / Robins, Amber (Dancer) / Sobotnik, Morton (Musician) / Morita, Theodora (Musician) / Pitot, Genevieve (Musician) / Juste, Roxane D'Orleans (Dancer) / Kenton, Stan (Musician) / Rugolo, Pete (Musician) / Swayze, William (Sound designer)
Created2005
Description
The ASU School of Dance presents New Danceworks I, October 20-23, with works by dance graduate students, performed at the Dance Studio Theatre, PEBE 132.
ContributorsBlake, Casey (Choreographer) / Niehaus, AJ (Choreographer, Composer, Costume designer, Dancer, Set designer) / Fellenz, Aaron (Lighting designer) / Niehaus, Susan (Costume designer) / Bingle, Meghan (Dancer) / Rajko, Stjepan (Dancer, Dancer) / Ryan, Rachel (Dancer) / Wong, Siew (Dancer) / Hart, Keira (Choreographer, Lighting designer, Set designer, Dancer) / Ammerman, Mark C. (Lighting designer, Technical director) / Holcman, Sarah (Dancer, Performer) / Kriston, Jordan (Dancer) / Lederman, Christiana (Dancer) / McGloin, Aaron (Dancer, Performer) / Iglesias, Alberto (Musician) / Burnett, Cherie (Dancer) / Kelley, Jean (Dancer) / Kemmerer, Shelley (Dancer) / Piver, Harper (Dancer) / Richardson, Katheryn (Dancer) / Young, Liz (Choreographer, Costume designer) / Stevens, Sufjan (Composer) / Nielsdottir, Sigridur (Composer) / Shustankovich, Dmitri (Composer) / Everett, Mark Oliver (Composer) / Wainright, Rufus (Composer) / Coates, Steven (Composer) / Swayze, William (Sound designer) / Atwood, Laura (Lighting designer, Stage manager) / Burns, Tuey (Performer) / Howe, Martha (Performer) / Kenworthy, Julianna (Performer) / Maywald, Marilyn (Performer) / Parish, Sara (Performer) / Richardson, Kathryn (Performer) / Wajih, Sonja (Performer) / Tsukayama, Jennifer (Artistic director) / Benard, Jacqueline (Costume designer) / Galina, Mihaleva (Costume designer)
Created2005
Description
The ASU School of Dance presents Sharing Unimaginable Worlds: Spring Concert 2006, April 27-30, with works by dance faculty, alumni, graduate, undergraduate, and visiting artists, performed at Galvin Playhouse.
ContributorsBauer, Lindsey (Choreographer, Costume designer) / Atwood, Laura (Composer) / Vessey, Julia (Lighting designer, Dancer) / Adams, Shardai (Dancer) / Allred, Candice (Dancer) / Braun, Cc (Dancer) / Caban, Francesco (Dancer) / Evans, Laura (Mitchell) (Dancer) / Felix, Steven (Dancer) / Fisher, Rose (Dancer) / Gill, Kimisha (Dancer) / Howe, Martha E. (Dancer) / Hutchinson, Kade (Dancer) / Jones, Monique (Dancer) / McCaskill, Katie (Dancer) / McDonald, Maria (Dancer) / Manus, Nicole (Dancer) / Nielsen, Erica (Dancer) / Rajko, Stjepan (Dancer) / Robinette, Danae (Dancer) / Ross, Janie (Dancer) / Ryan, Rachel (Dancer) / Verrelli, Emily (Dancer) / Willis, Blaire (Dancer) / Zaksek, Brittany (Dancer) / Zeims, Ellie (Dancer) / Karpanty, Kimberly (Choreographer) / Ammerman, Mark C. (Lighting designer, Technical director, Lighting designer, Set designer) / Benard, Jacqueline (Costume designer) / Mihaleva, Galina (Costume designer, Costume designer) / Burnett, Cherie (Dancer) / Dessingue, Colette (Dancer) / Kriston, Jordan (Dancer) / Lathrop, Cerrin (Dancer) / Roberts, Cassie (Dancer) / Sedlack, Kate (Dancer) / Gandolfi, Tristan (Dancer) / Kettner, Lauren (Dancer) / Koretski, Marissa (Dancer) / Kuo, Alexis I-Cheng (Dancer) / McGloin, Aaron (Dancer, Lighting designer) / Reker, Steven (Dancer) / Scott, Jillian (Dancer, Choreographer, Dancer) / Schupp, Karen (Choreographer, Dancer) / Koch, Carolyn (Lighting designer) / Vanier, Luc (Director, Choreographer) / Burns, Christopher (Musician) / Maruszewski, Evan (Animator) / Ma, Shouze (Choreographer, Dancer, Artistic director) / Pärt, Arvo (Musician) / Bruch, Max (Musician) / Blukis, Audrey (Dancer) / Kelley, Jean (Dancer) / Parish, Sara (Dancer) / Bingle, Meghan (Choreographer, Dancer) / Flanagan, Mollie (Lighting designer) / Harrison, Lauren (Dancer) / Tonding, Tara (Dancer) / Lavista, Claudia (Choreographer, Costume designer) / Ruiz, Victor Manuel (Choreographer, Lighting designer, Costume designer) / Garcia, Jesus "Chui" (Musician) / Hart, Keira (Dancer) / Kemmerer, Shelley (Dancer) / Kenworthy, Julianna (Dancer) / Lederman, Christiana (Dancer) / Milam, Nichole (Dancer) / Richardson, Kathryn (Dancer) / Smith, Vanessa (Dancer) / Swayze, William (Sound designer) / Herberger Institute School of Dance (Musician)
Created2006
Description
The ASU School of Dance presents Poetry Both Fierce and Fragile: Spring Concert, April 19-22, with works by guest artists, dance faculty, graduate, and undergraduate students, performed at Galvin Playhouse.
ContributorsMesman, Travis (Choreographer, Dancer) / Coltrane, Ravi (Musician, Performer) / Perdomo, Luis (Performer) / Gress, Drew (Performer) / Strickland, E.J. (Performer) / Benard, Jacqueline (Costume designer) / Mihaleva, Galina (Costume designer) / Sandstrom, Philip W. (Lighting designer) / Nagrin, Daniel (Director) / Tovson, Kristin (Choreographer) / Basting, Samantha (Dancer) / Malan-McDonald, Sara (Dancer) / Mapes, Aileen (Dancer) / Keuter, Cliff (Choreographer, Set designer, Costume designer) / Kaplan, Robert (Musician) / Parish, Sara (Costume designer, Dancer) / Ammerman, Mark C. (Lighting designer, Set designer, Technical director) / Moses, Robert (Choreographer) / Branca, Glenn (Musician) / Cardoza, Alicia (Dancer) / Howe, Martha E. (Dancer) / Massiah, Monique (Dancer) / Doherty, Kelley (Dancer) / Harrison, Christina (Dancer, Performer) / Mooney, Elina (Choreographer, Artistic director) / Ettinger, John (Musician) / Hester, Michael (Musician) / Smith, J. B. (Musician) / Blumenfeld-Jones, Donald (Dancer) / Ma, Shouze (Dancer) / Limon, Jose (Choreographer) / Kodaly, Zoltan (Musician) / Watt, Nina (Director) / Lee, Ming Cho (Costume designer, Set designer) / McGloin, Aaron (Performer) / Balderamma, Andrew (Performer) / Baum, Doug (Performer) / Johnson, George (Performer, Performer) / McNamara, Caitlyn (Performer) / Sheldrick, Sarah (Performer) / Spondello, Alison (Performer) / Grabionowski, Nicole (Performer) / Hancock, Whitney (Performer) / Hauk, Chelsey (Performer) / Kosowski, Jenna (Performer) / Wooldridge, Holly (Performer) / Evans, Laura (Performer) / Mollicone, Karissa (Performer) / Felix, Steven (Performer) / Trujillo, Davey (Performer) / Bormann, Megan (Performer) / Clegg, Brittany (Performer) / DeVries, Debbie (Performer) / Sakolsky-Basquill, Lydia (Performer) / Schwab, Katies (Performer) / Koch, Carolyn (Production manager) / Swayze, William (Sound designer)
Created2007