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Description
Antibodies are essential for structural determinations and functional studies of membrane proteins, but antibody generation is limited by the availability of properly-folded and purified antigen. We describe the first application of genetic immunization to a structurally diverse set of membrane proteins to show that immunization of mice with DNA alone

Antibodies are essential for structural determinations and functional studies of membrane proteins, but antibody generation is limited by the availability of properly-folded and purified antigen. We describe the first application of genetic immunization to a structurally diverse set of membrane proteins to show that immunization of mice with DNA alone produced antibodies against 71% (n = 17) of the bacterial and viral targets. Antibody production correlated with prior reports of target immunogenicity in host organisms, underscoring the efficiency of this DNA-gold micronanoplex approach. To generate each antigen for antibody characterization, we also developed a simple in vitro membrane protein expression and capture method. Antibody specificity was demonstrated upon identifying, for the first time, membrane-directed heterologous expression of the native sequences of the FopA and FTT1525 virulence determinants from the select agent Francisella tularensis SCHU S4. These approaches will accelerate future structural and functional investigations of therapeutically-relevant membrane proteins.
ContributorsHansen, Debra (Author) / Robida, Mark (Author) / Craciunescu, Felicia (Author) / Loskutov, Andrey (Author) / Dorner, Katerina (Author) / Rodenberry, John-Charles (Author) / Wang, Xiao (Author) / Olson, Tien (Author) / Patel, Hetal (Author) / Fromme, Petra (Author) / Sykes, Kathryn (Author) / Biodesign Institute (Contributor) / Innovations in Medicine (Contributor) / Applied Structural Discovery (Contributor) / College of Liberal Arts and Sciences (Contributor) / School of Molecular Sciences (Contributor)
Created2016-02-24
Description

The ASU School of Dance presents Emerging Artists I, February 9-12, with works by guest artists, dance faculty, and students, performed at Dance Studio Theatre, PEBE 132.

ContributorsSedlack, Kate (Choreographer, Lighting designer, Costume designer) / Bormann, Megan (Dancer) / Brack, Ashley (Dancer) / Hauk, Chelsey (Dancer) / Koeppel, Alex (Dancer) / Pesque, Julia (Dancer) / Roberts, Cassie (Dancer) / Roethler, Eva (Dancer) / Spenceley, Jenni (Dancer) / Trujillo, David (Dancer) / Fitzgerald, Mary (Artistic director, Choreographer, Lighting designer, Costume designer) / Ammerman, Mark C. (Technical director, Set designer, Lighting designer) / Benard, Jacqueline (Costume designer) / Mihaleva, Galina (Costume designer) / Swayze, William (Sound designer, Composer) / Archambault, Jared (Stage manager) / King, Natalie (Dancer) / Skrentny, Adrian (Choreographer, Lighting designer, Costume designer, Set designer, Dancer) / Bajromovic, Saban (Musician) / Parsons, Niamh (Musician) / Cortez, Sarah (Dancer) / Galindo, Rolanda (Dancer) / Kenworthy, Julianna (Dancer, Choreographer, Costume designer) / Kriston, Jordan (Dancer) / Wong, Jennifer (Dancer) / Taylor, Michelle (Choreographer) / Hart, Keira (Lighting designer) / Smith, Vanessa (Costume designer, Dancer) / Chesley, Andrea (Choreographer, Lighting designer, Costume designer, Dancer) / Chappman, Richard (Composer, Performer) / Childers, Emily (Dancer) / Cortez, Maggie (Dancer) / Howe, Martha E. (Choreographer) / Blake, Casey (Lighting designer) / Blukis, Audrey (Dancer) / Holcman, Sarah (Choreographer, Lighting designer, Costume designer, Set designer) / Lecuona, Margarita (Musician) / Santaolalla, Gustavo (Musician) / Aste, Cuti (Musician) / Lobos, Jorge (Musician) / Zamora, Maria Esther (Musician) / González, Polito (Musician) / Lindl, Roberto (Musician) / Sternke, Amy (Dancer) / Anderson, Laurie (Musician) / McGloin, Aaron (Lighting designer) / Bingle, Meghan (Dancer) / Jordan, Paul (Dancer) / Kuo, Alexis I-Cheng (Dancer) / Lederman, Christiana (Dancer) / Lerner, Kristin (Dancer) / Parish, Sara (Dancer) / Hatzis, Amy E. (Choreographer, Composer, Costume designer, Lighting designer) / Dumont, Marguerite (Dancer) / Ebling, Brett (Dancer) / Kettner, Lauren (Dancer) / Pannier, Nicci (Dancer) / Kelley, Jean (Costume designer, Dancer) / Monk, Meredith (Composer) / Kriston, Micah (Choreographer, Lighting designer, Costume designer) / Fox, Stacey (Cinematographer) / Herberger Institute School of Dance (Musician)
Created2006
Description

The ASU School of Dance presents Rhythms of Life, November 3-5, with works by guest artists performed at Dance Studio Theatre, PEBE 132.

ContributorsVissicaro, Pegge (Artistic director) / Mooney, Elina (Artistic director) / Koch, Carolyn (Production manager) / Ammerman, Mark C. (Technical director, Lighting designer, Set designer) / Mihaleva, Galina (Costume designer) / Benard, Jacqueline (Costume designer) / Swayze, William (Sound designer) / Valentin-Martinez, Joel (Artistic director, Choreographer) / Amegago, Modesto Mawulolo (Performer) / Chipaumire, Nora (Performer, Choreographer) / Butty, Tamba (Director, Performer) / Bodou, Princess (Performer) / Byne, Princess (Performer) / Dogbeh, Decontee (Performer) / Fredericks, Roseline (Performer) / Gaie, Aaron (Performer) / Morris, Janet (Performer) / Morris, Musa (Performer) / Morris, Musu (Performer) / Morris, Peace (Performer) / Morris, Viddy (Performer) / Nyankum, William (Performer) / Toe, Eliza (Performer) / Wallace, Chuco Glen (Performer) / Caldee, Víctor (Director, Performer) / Cactellanos, Yaisa Pérez (Performer) / Rodíguez, Edwin J (Performer) / Alvarez, Idalberto (Performer) / Becerra, Jhon (Performer) / Domínguez, Miguel (Performer) / Wilson, Sule Greg C. (Choreographer, Performer) / Downs, Lila (Musician) / Milam, Nichole (Dancer) / Verrelli, Emily (Dancer) / Yanez, Avery (Dancer) / Silvestre, Rosangela (Choreographer) / Lunn, Dani (Dancer, Dancer) / Badaró, Luiz (Musician) / Edwards, Margit (Dancer) / Brundage, Kirk (Musician) / Pallais, Mario (Musician) / Moraes, Katia (Vocalist deprecated, use Singer)) / dos Santos, Maria Lourdes Silvestre (Costume designer) / Williams, Shelby (Dancer) / Jones, Erica (Dancer) / Abdullah, Laila (Dancer) / Mullen, Kimberly (Dancer) / Yulin, Linda (Dancer) / Oliveira, Valter (Costume designer)
Created2006
Description

The ASU School of Dance presents Celebrating the Nagrin Legend, September 16-18, with works by dance faculty and visiting artists, performed at the Galvin Playhouse Theatre.

ContributorsNagrin, Daniel (Choreographer) / O'Hara, Shane (Performer) / Mooney, Elina (Choreographer, Dancer, Artistic director) / Cage, John (Musician) / Bolcom, William (Musician) / Ammerman, Mark C. (Set designer, Technical director) / Mihaleva, Galina (Costume designer, Set designer) / Benard, Jacqueline (Costume designer, Set designer) / Waters, Renee (Dancer) / Keuter, Cliff (Choreographer) / Chopin, Frederic (Musician) / Ammerman, Candace (Dancer) / Robins, Amber (Dancer) / Sobotnik, Morton (Musician) / Morita, Theodora (Musician) / Pitot, Genevieve (Musician) / Juste, Roxane D'Orleans (Dancer) / Kenton, Stan (Musician) / Rugolo, Pete (Musician) / Swayze, William (Sound designer)
Created2005
Description

The ASU School of Dance presents New Danceworks I, October 20-23, with works by dance graduate students, performed at the Dance Studio Theatre, PEBE 132.

ContributorsBlake, Casey (Choreographer) / Niehaus, AJ (Choreographer, Composer, Costume designer, Dancer, Set designer) / Fellenz, Aaron (Lighting designer) / Niehaus, Susan (Costume designer) / Bingle, Meghan (Dancer) / Rajko, Stjepan (Dancer, Dancer) / Ryan, Rachel (Dancer) / Wong, Siew (Dancer) / Hart, Keira (Choreographer, Lighting designer, Set designer, Dancer) / Ammerman, Mark C. (Lighting designer, Technical director) / Holcman, Sarah (Dancer, Performer) / Kriston, Jordan (Dancer) / Lederman, Christiana (Dancer) / McGloin, Aaron (Dancer, Performer) / Iglesias, Alberto (Musician) / Burnett, Cherie (Dancer) / Kelley, Jean (Dancer) / Kemmerer, Shelley (Dancer) / Piver, Harper (Dancer) / Richardson, Katheryn (Dancer) / Young, Liz (Choreographer, Costume designer) / Stevens, Sufjan (Composer) / Nielsdottir, Sigridur (Composer) / Shustankovich, Dmitri (Composer) / Everett, Mark Oliver (Composer) / Wainright, Rufus (Composer) / Coates, Steven (Composer) / Swayze, William (Sound designer) / Atwood, Laura (Lighting designer, Stage manager) / Burns, Tuey (Performer) / Howe, Martha (Performer) / Kenworthy, Julianna (Performer) / Maywald, Marilyn (Performer) / Parish, Sara (Performer) / Richardson, Kathryn (Performer) / Wajih, Sonja (Performer) / Tsukayama, Jennifer (Artistic director) / Benard, Jacqueline (Costume designer) / Galina, Mihaleva (Costume designer)
Created2005
Description

The ASU School of Dance presents Sharing Unimaginable Worlds: Spring Concert 2006, April 27-30, with works by dance faculty, alumni, graduate, undergraduate, and visiting artists, performed at Galvin Playhouse.

ContributorsBauer, Lindsey (Choreographer, Costume designer) / Atwood, Laura (Composer) / Vessey, Julia (Lighting designer, Dancer) / Adams, Shardai (Dancer) / Allred, Candice (Dancer) / Braun, Cc (Dancer) / Caban, Francesco (Dancer) / Evans, Laura (Mitchell) (Dancer) / Felix, Steven (Dancer) / Fisher, Rose (Dancer) / Gill, Kimisha (Dancer) / Howe, Martha E. (Dancer) / Hutchinson, Kade (Dancer) / Jones, Monique (Dancer) / McCaskill, Katie (Dancer) / McDonald, Maria (Dancer) / Manus, Nicole (Dancer) / Nielsen, Erica (Dancer) / Rajko, Stjepan (Dancer) / Robinette, Danae (Dancer) / Ross, Janie (Dancer) / Ryan, Rachel (Dancer) / Verrelli, Emily (Dancer) / Willis, Blaire (Dancer) / Zaksek, Brittany (Dancer) / Zeims, Ellie (Dancer) / Karpanty, Kimberly (Choreographer) / Ammerman, Mark C. (Lighting designer, Technical director, Lighting designer, Set designer) / Benard, Jacqueline (Costume designer) / Mihaleva, Galina (Costume designer, Costume designer) / Burnett, Cherie (Dancer) / Dessingue, Colette (Dancer) / Kriston, Jordan (Dancer) / Lathrop, Cerrin (Dancer) / Roberts, Cassie (Dancer) / Sedlack, Kate (Dancer) / Gandolfi, Tristan (Dancer) / Kettner, Lauren (Dancer) / Koretski, Marissa (Dancer) / Kuo, Alexis I-Cheng (Dancer) / McGloin, Aaron (Dancer, Lighting designer) / Reker, Steven (Dancer) / Scott, Jillian (Dancer, Choreographer, Dancer) / Schupp, Karen (Choreographer, Dancer) / Koch, Carolyn (Lighting designer) / Vanier, Luc (Director, Choreographer) / Burns, Christopher (Musician) / Maruszewski, Evan (Animator) / Ma, Shouze (Choreographer, Dancer, Artistic director) / Pärt, Arvo (Musician) / Bruch, Max (Musician) / Blukis, Audrey (Dancer) / Kelley, Jean (Dancer) / Parish, Sara (Dancer) / Bingle, Meghan (Choreographer, Dancer) / Flanagan, Mollie (Lighting designer) / Harrison, Lauren (Dancer) / Tonding, Tara (Dancer) / Lavista, Claudia (Choreographer, Costume designer) / Ruiz, Victor Manuel (Choreographer, Lighting designer, Costume designer) / Garcia, Jesus "Chui" (Musician) / Hart, Keira (Dancer) / Kemmerer, Shelley (Dancer) / Kenworthy, Julianna (Dancer) / Lederman, Christiana (Dancer) / Milam, Nichole (Dancer) / Richardson, Kathryn (Dancer) / Smith, Vanessa (Dancer) / Swayze, William (Sound designer) / Herberger Institute School of Dance (Musician)
Created2006
Description

The ASU School of Dance presents Poetry Both Fierce and Fragile: Spring Concert, April 19-22, with works by guest artists, dance faculty, graduate, and undergraduate students, performed at Galvin Playhouse.

ContributorsMesman, Travis (Choreographer, Dancer) / Coltrane, Ravi (Musician, Performer) / Perdomo, Luis (Performer) / Gress, Drew (Performer) / Strickland, E.J. (Performer) / Benard, Jacqueline (Costume designer) / Mihaleva, Galina (Costume designer) / Sandstrom, Philip W. (Lighting designer) / Nagrin, Daniel (Director) / Tovson, Kristin (Choreographer) / Basting, Samantha (Dancer) / Malan-McDonald, Sara (Dancer) / Mapes, Aileen (Dancer) / Keuter, Cliff (Choreographer, Set designer, Costume designer) / Kaplan, Robert (Musician) / Parish, Sara (Costume designer, Dancer) / Ammerman, Mark C. (Lighting designer, Set designer, Technical director) / Moses, Robert (Choreographer) / Branca, Glenn (Musician) / Cardoza, Alicia (Dancer) / Howe, Martha E. (Dancer) / Massiah, Monique (Dancer) / Doherty, Kelley (Dancer) / Harrison, Christina (Dancer, Performer) / Mooney, Elina (Choreographer, Artistic director) / Ettinger, John (Musician) / Hester, Michael (Musician) / Smith, J. B. (Musician) / Blumenfeld-Jones, Donald (Dancer) / Ma, Shouze (Dancer) / Limon, Jose (Choreographer) / Kodaly, Zoltan (Musician) / Watt, Nina (Director) / Lee, Ming Cho (Costume designer, Set designer) / McGloin, Aaron (Performer) / Balderamma, Andrew (Performer) / Baum, Doug (Performer) / Johnson, George (Performer, Performer) / McNamara, Caitlyn (Performer) / Sheldrick, Sarah (Performer) / Spondello, Alison (Performer) / Grabionowski, Nicole (Performer) / Hancock, Whitney (Performer) / Hauk, Chelsey (Performer) / Kosowski, Jenna (Performer) / Wooldridge, Holly (Performer) / Evans, Laura (Performer) / Mollicone, Karissa (Performer) / Felix, Steven (Performer) / Trujillo, Davey (Performer) / Bormann, Megan (Performer) / Clegg, Brittany (Performer) / DeVries, Debbie (Performer) / Sakolsky-Basquill, Lydia (Performer) / Schwab, Katies (Performer) / Koch, Carolyn (Production manager) / Swayze, William (Sound designer)
Created2007
Description

The ASU School of Dance presents Emerging Artists I, February 15-18, with works by guest artists, dance faculty, and undergrad students, performed at Dance Studio Theatre, PEBE 132.

ContributorsTsukayama, Jennifer (Choreographer) / Benard, Jacqueline (Costume designer) / Dessingue, Colette (Costume designer, Dancer) / Ammerman, Mark (Lighting designer, Set designer) / Burnett, Cherie (Dancer, Choreographer, Costume designer, Lighting designer) / Doherty, Kelly (Dancer) / Lathrop, Cerrin (Dancer, Choreographer, Costume designer, Lighting designer) / Scott, Jillian (Dancer, Choreographer, Costume designer, Lighting designer) / Happel, Nancy (Choreographer, Costume designer) / Brahem, Anauar (Musician) / Kuo, I-Wen (Costume designer) / McGloin, Aaron (Lighting designer, Stage manager) / Kuo, Alexis I-Cheng (Dancer) / Fitzgerald, Mary (Choreographer) / Albeniz, Isaac (Musician) / Nitz, Ryan (Performer) / Mihaleva, Galina (Costume designer) / Vessey, Julia (Lighting designer, Set designer) / Wajih, Sonja (Dancer) / Scott, Laurie (Lighting designer) / Faciana, Maria (Dancer) / Roberts, Cassandra (Dancer) / Millford, Tobie (Musician) / Massiah, Monique (Dancer) / Roberts, Cassie (Dancer) / Van Buskirk, Kelly (Dancer) / Allred, Candice (Choreographer, Costume designer, Lighting designer) / Adams, Shardai (Dancer) / Caron, Bridgette (Dancer) / Hammer, Michelle (Dancer) / Hughes, Whitney (Dancer) / Kozulla, Ashle (Dancer) / Blukis, Audrey (Dancer) / Mc Caskill, Katie (Dancer) / Mooney, Elina (Artistic director) / Koch, Carolyn (Production manager) / Ammerman, Mark C. (Technical director, Set designer) / Swayze, William (Sound designer) / Murphey, Claudia (Artistic director)
Created2007
Description

The ASU School of Dance presents Emerging Artists II, February 16-19, with works by dance faculty and undergrad students, performed at Dance Studio Theatre, PEBE 132.

ContributorsBingle, Meghan (Choreographer, Lighting designer, Costume designer, Dancer) / Anderson, Laurie (Composer) / Callandine, Tess (Dancer) / Fellenz, Aaron (Dancer) / Harrison, Lauren (Dancer) / Jordan, Paul (Dancer) / Lederman, Christiana (Dancer) / Richardson, Kathryn (Dancer, Costume designer) / Smith, Vanessa (Dancer, Dancer) / Tonding, Tara (Dancer, Dancer, Costume designer) / McMahan, Jeff (Choreographer, Director) / Reker, Steven (Costume designer, Dancer) / Kemmerer, Shelley (Choreographer, Musician, Lighting designer, Costume designer, Set designer, Dancer) / Ammerman, Mark C. (Set designer, Lighting designer, Technical director) / Harrison, Christina (Dancer) / Hart, Keira (Lighting designer, Dancer, Costume designer) / Maywald, Marilyn (Dancer, Choreographer, Lighting designer, Costume designer) / Young, Liz (Dancer) / Martinez, Meredith (Choreographer, Lighting designer, Costume designer, Dancer) / Martinez, Cristobal (Composer) / Allred, Candice (Dancer) / Robinett, Danae (Dancer) / Ross, Janie (Dancer) / Hall Mckee, Lisa (Choreographer) / Atwood, Laura (Lighting designer) / Lerner, Kristin (Choreographer, Performer, Lighting designer, Costume designer) / Krauss, Alison (Musician) / Harris, Emmylou (Musician) / Welch, Gillian (Musician) / Goldstein, Malcolm (Musician) / Kenworthy, Julianna (Dancer) / Kriston, Jordan (Dancer) / Sternke, Amy (Dancer) / Greer, Lindsey S. (Choreographer, Composer, Lighting designer, Costume designer) / Candler, Martha (Dancer) / Ebling, Brett (Dancer) / Fox, Megan (Dancer) / Hatzis, Amy E. (Dancer) / Mollicone, Karissa (Dancer) / Van Buskirk, Kelly (Dancer) / Hookala, Rayn (Choreographer, Costume designer, Set designer) / Kukes, Sarah (Lighting designer) / Mihaleva, Galina (Costume designer) / Carlsen, Christine (Videographer) / Schupp, Karen (Choreographer) / Addy, Obo (Composer) / Stevens, Sufjan (Musician) / McGloin, Aaron (Dancer) / Fitzgerald, Mary (Artistic director) / Benard, Jacqueline (Costume designer) / Swayze, William (Sound designer) / Vessey, Julia (Stage manager)
Created2006
Description

The ASU School of Dance presents Graduate Showings, March 8, with works by dance graduate students, performed at the Dance Studio Theatre, PEBE 132.

ContributorsWright, Emily (Choreographer) / Ma, Yo Yo (Musician) / Manus, Nicole (Dancer, Speaker) / Ross, Janie (Dancer, Choreographer, Dancer, Artistic director) / Wentzel, Dana (Dancer) / Yanez, Avery (Dancer) / Braun, C-c (Choreographer, Dancer) / Caldwell, Nicole (Dancer) / Nielsen, Erica (Dancer) / Sena, Kathleen (Dancer) / Isham, Mark (Musician) / Biggs, Kristi (Dancer) / Cardoza, Alicia (Dancer) / Grejo, Trista (Dancer) / McNamara, Caitlyn (Dancer) / Jeanlouis, Toussaint (Dancer) / Schwab, Katie (Dancer) / Smith, Allison (Dancer) / Howe, Martha (Dancer) / Harrison, Christina (Choreographer, Dancer) / Parish, Sara (Dancer) / Kosowski, Jenna (Choreographer, Dancer) / Gerzso, Andrew (Musician) / Kang, Hae-Sun (Musician) / Brack, Ashley (Dancer) / Thorngren, Lisa (Dancer, Choreographer) / Vessey, Julia (Choreographer) / Davis, Miles (Musician) / Borman, Megan (Dancer) / Hosack, Brittany (Dancer) / Messiah, Monique (Dancer) / Mitchell, Laura M (Dancer) / Pearson, Amanda (Dancer) / Glass, Philip (Musician) / Piver, Harper (Choreographer, Dancer) / King, Natalie (Speaker) / Ammerman, Mark C. (Technical director) / Herberger Institute School of Dance (Producer)
Created2006