Matching Items (148)
Description
A coincidence reporter construct, consisting of the p21-promoter and two luciferase genes (Firefly and Renilla), was constructed for the screening of drugs that might inhibit Olig2's tumorigenic role in glioblastoma. The reporter construct was tested using an Olig2 inhibitor, HSP990, as well as short hairpin RNA targeting Olig2. Further confirmatory analysis is needed before the reporter cell line is ready for high-throughput screening at the NIH and lead compound selection.
ContributorsCusimano, Joseph Michael (Author) / LaBaer, Joshua (Thesis director) / Mangone, Marco (Committee member) / Mehta, Shwetal (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2014-05
Description
Background: Breast cancer is the most frequently diagnosed cancer and the leading cause of cancer deaths in females worldwide, accounting for 23% of all new cancer cases and 14% of all total cancer deaths in 2008. Five tumor-normal pairs of primary breast epithelial cells were treated for infinite proliferation by using a ROCK inhibitor and mouse feeder cells. Methods: Raw paired-end, 100x coverage RNA-Seq data was aligned to the Human Reference Genome Version 19 using BWA and Tophat. Gene differential expression analysis was completed using Cufflinks and Cuffdiff. Interactive Genome Viewer was used for data visualization. Results: 15 genes were found to be down-regulated by at least one log-fold change in 4/5 of tumor samples. 75 genes were found to be down-regulated in 3/5 of our tumor samples by at least one log-fold change. 11 genes were found to be up-regulated in 4/5 of our tumor samples, and 68 genes were identified to be up-regulated in 3/5 of the tumor samples by at least one-fold change. Conclusion: Expression changes in genes such as AZGP1, AGER, ALG11, and S1007 suggest a disruption in the glycosylation pathway. No correlation was found between Cufflink's Her2 gene-expression and DAKO score classification.
ContributorsHernandez, Fernando (Author) / Anderson, Karen (Thesis director) / Mangone, Marco (Committee member) / Park, Jin (Committee member) / Barrett, The Honors College (Contributor) / Department of Information Systems (Contributor)
Created2013-05
Description
The aim of this thesis was to explore whether major life changes can have a visual, perceptible impact on facial changes. The proposed mediation model suggested that changes in personality serve as a mediating factor between life experiences and facial changes throughout the lifetime. The proposed model was tested by examining (1) perceived personality changes, (2) perceived physical changes, and (3) major life changes in photos of individuals' old-aged faces compared to their respective younger faces. Participants in the current study viewed old and young photos of 29 Miss America pageant winners and rated how much each older face changed from its respective younger face on the following criteria: age change, overall change, personality change, and physical change. Responses were aggregated across participants for each target, and personality and physical items were separately composited into single measures of overall perceived personality change and overall perceived physical change. Results did not support the proposed model; however, some marginally significant correlations were found between the number of times the targets experienced a change in marital status and the appearance of being calm, feminine, and less changed in older age. However, these correlations were in the reverse direction from what was expected; further research is needed to understand how marital changes influence, and are influenced by, personality and physical changes. As a form of face perception, the processes underlying the proposed model are discussed in terms of possible social consequences. Further research is needed to explore whether changes in life events, such as the ones presented here, are related to specific facets of personality and physical changes, and how these perceptions translate to important social outcomes. Suggestions for future research pertaining to these issues are discussed.
ContributorsMichael, Kendra (Author) / Kwan, Virginia (Thesis director) / Knight, George (Committee member) / Neuberg, Steven (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
What characteristics do people prefer in potential mates? Previous studies have explored this question, discovering that preferred characteristics vary by people's sex and sexual strategy, but have implied that these preferences remain constant across the lifespan. We suggest, however, that systematic variation exists in individuals' mate preferences across the lifespan, as they shift their investments from mating toward parenting. We suggest that the characteristics of a potential mate can be viewed as affordances that assist or hinder an individual in achieving certain fundamental goals. Incorporating the framework of Life History Theory with this affordance-management approach to social behavior, we propose that an individual's life stage, sex, and life history strategy together serve as the basis for these goals and thereby shape the characteristics people seek in potential mates. Using data collected from participants aged 18-45 recruited on Amazon's Mechanical Turk, we tested a range of hypotheses derived from our approach. In general, results provide mixed support for a role of life stage in shaping mate preferences. For example, nurturance and social competence were viewed as more necessary characteristics in a mate by participants invested in parenting. Moreover, as their investment in mating increased, females expressed a greater preference for ambition in their potential mates, but males did not. Other predictions were not borne out, however, suggesting that there is still much to be learned from investigating the relationship between life stage and mate preferences.
ContributorsKalina, Catherine Ellen (Author) / Neuberg, Steven (Thesis director) / Glenberg, Arthur (Committee member) / Gangestad, Steven (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor) / Graduate College (Contributor)
Created2014-05
Description
The United States has become home to the largest incarcerated population in the world, containing 25% of the world's prisoners (NAACP, 2013). Within this population, young men of color appear to be severely overrepresented. This phenomenon can be better understood with the aid of a multi-disciplinary approach within the social sciences. Evolutionary theory is combined with multiple psychological and sociological perspectives, in order to more deeply understand the multi-level intersection of prejudice and discrimination against society's disadvantaged or vulnerable populations. A synthesis of the multiple theoretical angles of social dominance theory, affordance management, and life history theory is used to suggest a threat-based, attributional framework for understanding punitive decision-making and policy support. This conceptualization also considers the importance of the legal system in effecting social change. Future research within the legal arena is recommended to enable a more refined understanding of punitive ideology and implicit bias within the criminal justice system.
ContributorsLeiferman, Lindsay M (Author) / Szeli, Ãâ°va (Thesis director) / Gómez, Alan (Committee member) / Neuberg, Steven (Committee member) / Barrett, The Honors College (Contributor) / School of Social Transformation (Contributor) / Department of Psychology (Contributor)
Created2014-05
Description
miRNAs are short non-coding regulatory RNAs that have an important roles in a wide range of biological processes. Dysfunction of miRNA regulation has also been shown to occur in diseases such as cancer. Despite the widespread influence of miRNAs in these contexts, the vast majority of miRNA targets are poorly characterized. The aim of this research project was to gain a better understating of miRNA targeting by using the model organism C. elegans. In order to do this I adapted a novel high-throughput assay to detect miRNA targets for use with the C. elegans 3`UTRome. As a proof of principle I performed this assay on 96 C. elegans 3`UTRs using high-throughput techniques. The results revealed miRNA interactions with two predicted 3`UTR targets for the miRNA lin-4 and ten unpredicted targets. The results also corroborated previous findings that certain worm miRNAs require special modifications to be expressed in human cells.
ContributorsKotagama, Kasuen Indrajith Bandara (Author) / Mangone, Marco (Thesis director) / Anderson, Karen (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2013-12
Description
Alternative polyadenylation (APA) is the biological mechanism in which the same gene can have multiple 3'untranslated region (3'UTR) isoforms due to the presence of multiple polyadenylation signal (PAS) elements within the pre mRNAs. Because APA produces mRNA transcripts that have different 3'UTR isoforms, certain transcripts may be subject to post-transcriptional regulation by regulatory non-coding RNAs, such as microRNAs or RNA binding proteins defects of which have been implicated in diseases such as cancer. Despite the increasing level of information, functional understanding of the molecular mechanisms involved in transcription is still poorly understood, nor is it clear why APA is necessary at a cell or tissue-specific level. To address these questions I wanted to develop a set of sensor strain plasmids capable of detecting cleavage and polyadenylation in vivo, inject the complete sensor strain plasmid into C. elegans and prepare stable transgenic lines, and perform proof-of-principle RNAi feeding experiments targeting genes associated with the cleavage and polyadenylation complex machinery. I demonstrated that it was possible to create a plasmid capable of detecting cleavage and polyadenylation in C. elegans; however, issues arose during the RNAi assays indicating the sensor strain plasmid was not sensitive enough to the RNAi to effectively detect in the worms. Once the problems involved with sensitivity and variability in the RNAi effects are resolved, the plasmid would be able to better address questions regarding the functional understanding of molecular mechanisms involved in transcription termination.
ContributorsWilky, Henry Patrick (Author) / Mangone, Marco (Thesis director) / Newbern, Jason (Committee member) / Blazie, Stephen (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
A prior experiment by Li and colleagues found that when participants rated same sex faces in physical attractiveness, their self-reports of religiosity were higher in comparison to those that rated opposite sex faces. Could this be due to participants feeling their sexuality was threatened or misunderstood? In the current experiment, we attempted to replicate these findings and extend them by using a pseudo personality test that presented false feedback to participants. This feedback explained that their personalities were similar to homosexual or heterosexual people. Four hundred and fifty participants from Amazon Mturk were randomized into these conditions. We also measured homophobia, moral values, and the believability of the experiment. Results displayed no replication of the original findings. Men were more homophobic than women, while displaying lower moral values and religiosity. Those that self-reported being more homophobic also reported being more religious and moral. In conditions of sexual threat (homosexual personality, same sex faces) and sexual comfort (heterosexual personality, opposite sex faces), self-reports of moral values increased. Participants that reported believing the feedback displayed higher religiosity in both sexual threat and sexual comfort conditions. For a more concrete understanding of the relationship between religiosity, mating goals, and threats to sexuality, more research needs to be performed.
ContributorsHobaica, Steven Matthew (Author) / Cohen, Adam (Thesis director) / Knight, George (Committee member) / Neuberg, Steven (Committee member) / Barrett, The Honors College (Contributor) / School of International Letters and Cultures (Contributor) / Department of Psychology (Contributor) / College of Public Programs (Contributor)
Created2014-12
Description
The purpose of this thesis study was to examine whether the "war on cancer" metaphor influences cancer perception and treatment decision. A total of 249 undergraduates (152 females) from a large southwestern university participated in an online survey experiment and were either randomly assigned to the control condition (N=123) or to the war prime condition (N=126). Participants in the control condition did not receive the metaphor manipulation while participants in the war prime condition received the subtle "war on cancer" metaphor prime. After the prime was given, participants read a scenario, answered questions related to the situation, and responded to demographic questions. The results suggested that, compared to participants in the no-prime condition, participants exposed to the war metaphor were more likely to (a) view melanoma as an acute disease, (b) choose chemotherapy over molecular tests, and (c) prefer more aggressive treatment. These findings illustrated the unintended consequences of the "war on cancer" slogan. The results were encouraging and in the predicted direction, but the effect size was small. The discussion section described possible future directions for research.
ContributorsShangraw, Ann Mariah (Author) / Kwan, Virginia (Thesis director) / Neuberg, Steven (Committee member) / Cavanaugh Toft, Carolyn (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor)
Created2015-05
Description
Previous studies exploring variability in sentencing decisions have consistently found gender differences, such that women receive lighter sentences than men. In the proposed study, I present a new framework for understanding gender differences in sentencing preferences, including circumstances under which no gender differences should emerge. The Affordance Management Approach suggests that our minds are attuned to both group- and individual-level threats and opportunities that others afford us. I conceptualize the sentencing difference between men and women as driven by perceived affordances that assist or hinder an individual in achieving certain fundamental goals. When faced with sanctioning an offender in our community, the offender's sex, the victim's age, and environmental variables such as the ratio of men to women may influence our decision-making, because these factors have affordance implications. Thus, I hypothesized that individuals will express differences in the sentencing of offenders who commit assault, and that these differences vary by offender sex, victim age, and sex-ratio. The results indicate that, as predicted, female offenders received lighter sentencing than men when the offender committed an assault against a same-sex adult, but received equally punitive sentences as men when the assault was committed against a child. In general, results do not support a consistent effect of sex ratio as a factor when making sentencing decisions. Although results do not fully support the current study's specific hypotheses, there remains much to be gained from applying an affordance management perspective to understanding variability in sentencing between the sexes.
ContributorsUzzanti, Charlene Ann (Author) / Neuberg, Steven (Thesis director) / Knight, George (Committee member) / Salerno, Jessica (Committee member) / Barrett, The Honors College (Contributor) / School of Criminology and Criminal Justice (Contributor) / Department of Psychology (Contributor)
Created2015-05