Matching Items (67)
Description
The RAS/MAPK (RAS/Mitogen Activated Protein Kinase) pathway is a highly conserved, canonical signaling cascade that is highly involved in cellular growth and proliferation as well as cell migration. As such, it plays an important role in development, specifically in development of the nervous system. Activation of ERK is indispensable for the differentiation of Embryonic Stem Cells (ESC) into neuronal precursors (Li z et al, 2006). ERK signaling has also shown to mediate Schwann cell myelination of the peripheral nervous system (PNS) as well as oligodendrocyte proliferation (Newbern et al, 2011). The class of developmental disorders that result in the dysregulation of RAS signaling are known as RASopathies. The molecular and cell-specific consequences of these various pathway mutations remain to be elucidated. While there is evidence for altered DNA transcription in RASopathies, there is little work examining the effects of the RASopathy-linked mutations on protein translation and post-translational modifications in vivo. RASopathies have phenotypic and molecular similarities to other disorders such as Fragile X Syndrome (FXS) and Tuberous Sclerosis (TSC) that show evidence of aberrant protein synthesis and affect related pathways. There are also well-defined downstream RAS pathway elements involved in translation. Additionally, aberrant corticospinal axon outgrowth has been observed in disease models of RASopathies (Xing et al, 2016). For these reasons, this present study examines a subset of proteins involved in translation and translational regulation in the context of RASopathy disease states. Results indicate that in both of the tested RASopathy model systems, there is altered mTOR expression. Additionally the loss of function model showed a decrease in rps6 activation. This data supports a role for the selective dysregulation of translational control elements in RASopathy models. This data also indicates that the primary candidate mechanism for control of altered translation in these modes is through the altered expression of mTOR.
ContributorsHilbert, Alexander Robert (Author) / Newbern, Jason (Thesis director) / Olive, M. Foster (Committee member) / Bjorklund, Reed (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2017-05
Description
For centuries, religion has been a part of people's lives, impacting their well-being. Well-being includes both positive and negative emotions that make people feel satisfied. Religion can affect how people handle their physical health, social behavior, support, and stress. Interestingly, more people have been abandoning religion since 1990, even though it has positive effects on well-being. In this study, I sought to understand the extent to which leaving religion is associated with all aspects of well-being, including spiritual well-being. I asked 606 Arizona State University undergraduates to rate their well-being from two different time periods in their life. Participants were first asked if they were currently religious or nonreligious;135 said they had left/changed religions. If participants indicated that they did not leave/change religions, they were asked about their well-being before and after starting at ASU. I examined perceived change in well-being to understand how religion can influence many aspects of an individual’s life. Leaving or changing religion was positively associated with well-being. Those who reported leaving religion reported better environmental, emotional, social, educational, occupational, and spiritual well-being. In the control group (ASU), educational and spiritual well-being had a significant increase, which is interesting. The results showed that changing/leaving religion had a positive association on well-being.
ContributorsCarbine, Analy (Author) / Cohen, Adam (Thesis director) / Corbin, William (Committee member) / Johnson, Kathryn (Committee member) / Barrett, The Honors College (Contributor) / Sanford School of Social and Family Dynamics (Contributor) / Department of Psychology (Contributor)
Created2023-12
Description
The future of driving is largely headed towards autonomous vehicles, and this is clear with companies such as Tesla, Waymo, and even tech giant Apple. Many professionals predict that autonomous vehicles will likely be commercially available and legal to use in some places by the late 2020s [15]. There are some benefits to the rapid development of autonomous vehicle controllers, such as more independence for those who can’t drive due to impairments, the potential for reduced traffic, as well as possibly decreasing the number of accidents. Though these are promising prospects, there are ethical concerns regarding the implementation of such technology. The goal of this thesis is to provide an introductory literature review that discusses the history of autonomous vehicles, different levels of autonomy, ethical considerations in autonomous systems, and prior work on characterizing human driving behaviors and implementing these behaviors with autonomous vehicle controllers. Finally, recommendations are proposed for data collection on human driving behaviors in an ongoing NSF-funded project at Arizona State University, “Embodiment of Human Values Profiles in Autonomous Vehicles via Psychomimetic Controller Design.”
ContributorsYoung, Brittine (Author) / Berman, Spring (Thesis director) / Johnson, Kathryn (Committee member) / Barrett, The Honors College (Contributor) / Department of Physics (Contributor) / Historical, Philosophical & Religious Studies, Sch (Contributor) / Mechanical and Aerospace Engineering Program (Contributor)
Created2022-05
Description
This dissertation describes a series of four studies on cognitive aging, working memory, and cognitive flexibility in dogs (Canis lupus familiaris) and their wild relatives. In Chapters 2 and 3, I designed assessments for age-related cognitive deficits in pet dogs which can be deployed rapidly using inexpensive and accessible materials. These novel tests can be easily implemented by owners, veterinarians, and clinicians and therefore, may improve care for elderly dogs by aiding in the diagnosis of dementia. In addition, these widely deployable tests may facilitate the use of dementia in pet dogs as a naturally occurring model of Alzheimer’s Disease in humans.In Chapters 4 and 5, I modified one of these tests to demonstrate for the first time that coyotes (Canis latrans) and wolves (Canis lupus lupus) develop age-related deficits in cognitive flexibility. This was an important first step towards differentiating between the genetic and environmental components of dementia in dogs and in turn, humans. Unexpectedly, I also detected cognitive deficits in young, adult dogs and wolves but not coyotes. These finding add to a recent shift in understanding cognitive development in dogs which may improve cognitive aging tests as well as training, care, and use of working and pet dogs. These findings also suggest that the ecology of coyotes may select for flexibility earlier in development.
In Chapter 5, I piloted the use of the same cognitive flexibility test for red and gray foxes so that future studies may test for lifespan changes in the cognition of small-bodied captive canids. More broadly, this paradigm may accommodate physical and behavioral differences between diverse pet and captive animals. In Chapters 4 and 5, I examined which ecological traits drive the evolution of behavioral flexibility and in turn, species resilience. I found that wolves displayed less flexibility than dogs and coyotes suggesting that species which do not rely heavily on unstable resources may be ill-equipped to cope with human habitat modification. Ultimately, this comparative work may help conservation practitioners to identify and protect species that cannot cope with rapid and unnatural environmental change.
ContributorsVan Bourg, Joshua (Author) / Wynne, Clive D (Thesis advisor) / Aktipis, C. Athena (Committee member) / Gilby, Ian C (Committee member) / Young, Julie K (Committee member) / Arizona State University (Publisher)
Created2022
Description
The Autonomous Vehicle (AV), also known as self-driving car, promises to be a game changer for the transportation industry. This technology is predicted to drastically reduce the number of traffic fatalities due to human error [21].
However, road driving at any reasonable speed involves some risks. Therefore, even with high-tech AV algorithms and sophisticated sensors, there may be unavoidable crashes due to imperfection of the AV systems, or unexpected encounters with wildlife, children and pedestrians. Whenever there is a risk involved, there is the need for an ethical decision to be made [33].
While ethical and moral decision-making in humans has long been studied by experts, the advent of artificial intelligence (AI) also calls for machine ethics. To study the different moral and ethical decisions made by humans, experts may use the Trolley Problem [34], which is a scenario where one must pull a switch near a trolley track to redirect the trolley to kill one person on the track or do nothing, which will result in the deaths of five people. While it is important to take into account the input of members of a society and perform studies to understand how humans crash during unavoidable accidents to help program moral and ethical decision-making into self-driving cars, using the classical trolley problem is not ideal, as it is unrealistic and does not represent moral situations that people face in the real world.
This work seeks to increase the realism of the classical trolley problem for use in studies on moral and ethical decision-making by simulating realistic driving conditions in an immersive virtual environment with unavoidable crash scenarios, to investigate how drivers crash during these scenarios. Chapter 1 gives an in-depth background into autonomous vehicles and relevant ethical and moral problems; Chapter 2 describes current state-of-the-art online tools and simulators that were developed to study moral decision-making during unavoidable crashes. Chapters 3 focuses on building the simulator and the design of the crash scenarios. Chapter 4 describes human subjects experiments that were conducted with the simulator and their results, and Chapter 5 provides conclusions and avenues for future work.
However, road driving at any reasonable speed involves some risks. Therefore, even with high-tech AV algorithms and sophisticated sensors, there may be unavoidable crashes due to imperfection of the AV systems, or unexpected encounters with wildlife, children and pedestrians. Whenever there is a risk involved, there is the need for an ethical decision to be made [33].
While ethical and moral decision-making in humans has long been studied by experts, the advent of artificial intelligence (AI) also calls for machine ethics. To study the different moral and ethical decisions made by humans, experts may use the Trolley Problem [34], which is a scenario where one must pull a switch near a trolley track to redirect the trolley to kill one person on the track or do nothing, which will result in the deaths of five people. While it is important to take into account the input of members of a society and perform studies to understand how humans crash during unavoidable accidents to help program moral and ethical decision-making into self-driving cars, using the classical trolley problem is not ideal, as it is unrealistic and does not represent moral situations that people face in the real world.
This work seeks to increase the realism of the classical trolley problem for use in studies on moral and ethical decision-making by simulating realistic driving conditions in an immersive virtual environment with unavoidable crash scenarios, to investigate how drivers crash during these scenarios. Chapter 1 gives an in-depth background into autonomous vehicles and relevant ethical and moral problems; Chapter 2 describes current state-of-the-art online tools and simulators that were developed to study moral decision-making during unavoidable crashes. Chapters 3 focuses on building the simulator and the design of the crash scenarios. Chapter 4 describes human subjects experiments that were conducted with the simulator and their results, and Chapter 5 provides conclusions and avenues for future work.
ContributorsKankam, Immanuella (Author) / Berman, Spring (Thesis advisor) / Johnson, Kathryn (Committee member) / Yong, Sze Zheng (Committee member) / Arizona State University (Publisher)
Created2019
Description
Evidence from the 20th century demonstrated that early life stress (ELS) produces long lasting neuroendocrine and behavioral effects related to an increased vulnerability towards psychiatric illnesses such as major depressive disorder, post-traumatic stress disorder, schizophrenia, and substance use disorder. Substance use disorders (SUDs) are complex neurological and behavioral psychiatric illnesses. The development, maintenance, and relapse of SUDs involve multiple brain systems and are affected by many variables, including socio-economic and genetic factors. Pre-clinical studies demonstrate that ELS affects many of the same systems, such as the reward circuitry and executive function involved with addiction-like behaviors. Previous research has focused on cocaine, ethanol, opiates, and amphetamine, while few studies have investigated ELS and methamphetamine (METH) vulnerability. METH is a highly addictive psychostimulant that when abused, has deleterious effects on the user and society. However, a critical unanswered question remains; how do early life experiences modulate both neural systems and behavior in adulthood? The emerging field of neuroepigenetics provides a potential answer to this question. Methyl CpG binding protein 2 (MeCP2), an epigenetic tag, has emerged as one possible mediator between initial drug use and the transition to addiction. Additionally, there are various neural systems that undergo long lasting epigenetics changes after ELS, such as the response of the hypothalamo-pituitary-adrenal (HPA) axis to stressors. Despite this, little attention has been given to the interactions between ELS, epigenetics, and addiction vulnerability. The studies described herein investigated the effects of ELS on METH self-administration (SA) in adult male rats. Next, we investigated the effects of ELS and METH SA on MeCP2 expression in the nucleus accumbens and dorsal striatum. Additionally, we investigated the effects of virally-mediated knockdown of MeCP2 expression in the nucleus accumbens core on METH SA, motivation to obtain METH under conditions of increasing behavioral demand, and reinstatement of METH-seeking in rats with and without a history of ELS. The results of these studies provide insights into potential epigenetic mechanisms by which ELS can produce an increased vulnerability to addiction in adulthood. Moreover, these studies shed light on possible novel molecular targets for treating addiction in individuals with a history of ELS.
ContributorsLewis, Candace (Author) / Olive, M. Foster (Thesis advisor) / Hammer, Ronald (Committee member) / Neisewander, Janet (Committee member) / Sanabria, Federico (Committee member) / Arizona State University (Publisher)
Created2015
Description
Trees serve as a natural umbrella to mitigate insolation absorbed by features of the urban environment, especially building structures and pavements. For a desert community, trees are a particularly valuable asset because they contribute to energy conservation efforts, improve home values, allow for cost savings, and promote enhanced health and well-being. The main obstacle in creating a sustainable urban community in a desert city with trees is the scarceness and cost of irrigation water. Thus, strategically located and arranged desert trees with the fewest tree numbers possible potentially translate into significant energy, water and long-term cost savings as well as conservation, economic, and health benefits. The objective of this dissertation is to achieve this research goal with integrated methods from both theoretical and empirical perspectives.
This dissertation includes three main parts. The first part proposes a spatial optimization method to optimize the tree locations with the objective to maximize shade coverage on building facades and open structures and minimize shade coverage on building rooftops in a 3-dimensional environment. Second, an outdoor urban physical scale model with field measurement is presented to understand the cooling and locational benefits of tree shade. The third part implements a microclimate numerical simulation model to analyze how the specific tree locations and arrangements influence outdoor microclimates and improve human thermal comfort. These three parts of the dissertation attempt to fill the research gap of how to strategically locate trees at the building to neighborhood scale, and quantifying the impact of such arrangements.
Results highlight the significance of arranging residential shade trees across different geographical scales. In both the building and neighborhood scales, research results recommend that trees should be arranged in the central part of the building south front yard. More cooling benefits are provided to the building structures and outdoor microclimates with a cluster tree arrangement without canopy overlap; however, if residents are interested in creating a better outdoor thermal environment, open space between trees is needed to enhance the wind environment for better human thermal comfort. Considering the rapid urbanization process, limited water resources supply, and the severe heat stress in the urban areas, judicious design and planning of trees is of increasing importance for improving the life quality and sustaining the urban environment.
ContributorsZhao, Qunshan (Author) / Wentz, Elizabeth (Thesis advisor) / Sailor, David (Committee member) / Wang, Zhi-Hua (Committee member) / Arizona State University (Publisher)
Created2017
Description
Autonomous vehicle technology has been evolving for years since the Automated Highway System Project. However, this technology has been under increased scrutiny ever since an autonomous vehicle killed Elaine Herzberg, who was crossing the street in Tempe, Arizona in March 2018. Recent tests of autonomous vehicles on public roads have faced opposition from nearby residents. Before these vehicles are widely deployed, it is imperative that the general public trusts them. For this, the vehicles must be able to identify objects in their surroundings and demonstrate the ability to follow traffic rules while making decisions with human-like moral integrity when confronted with an ethical dilemma, such as an unavoidable crash that will injure either a pedestrian or the passenger.
Testing autonomous vehicles in real-world scenarios would pose a threat to people and property alike. A safe alternative is to simulate these scenarios and test to ensure that the resulting programs can work in real-world scenarios. Moreover, in order to detect a moral dilemma situation quickly, the vehicle should be able to identify objects in real-time while driving. Toward this end, this thesis investigates the use of cross-platform training for neural networks that perform visual identification of common objects in driving scenarios. Here, the object detection algorithm Faster R-CNN is used. The hypothesis is that it is possible to train a neural network model to detect objects from two different domains, simulated or physical, using transfer learning. As a proof of concept, an object detection model is trained on image datasets extracted from CARLA, a virtual driving environment, via transfer learning. After bringing the total loss factor to 0.4, the model is evaluated with an IoU metric. It is determined that the model has a precision of 100% and 75% for vehicles and traffic lights respectively. The recall is found to be 84.62% and 75% for the same. It is also shown that this model can detect the same classes of objects from other virtual environments and real-world images. Further modifications to the algorithm that may be required to improve performance are discussed as future work.
Testing autonomous vehicles in real-world scenarios would pose a threat to people and property alike. A safe alternative is to simulate these scenarios and test to ensure that the resulting programs can work in real-world scenarios. Moreover, in order to detect a moral dilemma situation quickly, the vehicle should be able to identify objects in real-time while driving. Toward this end, this thesis investigates the use of cross-platform training for neural networks that perform visual identification of common objects in driving scenarios. Here, the object detection algorithm Faster R-CNN is used. The hypothesis is that it is possible to train a neural network model to detect objects from two different domains, simulated or physical, using transfer learning. As a proof of concept, an object detection model is trained on image datasets extracted from CARLA, a virtual driving environment, via transfer learning. After bringing the total loss factor to 0.4, the model is evaluated with an IoU metric. It is determined that the model has a precision of 100% and 75% for vehicles and traffic lights respectively. The recall is found to be 84.62% and 75% for the same. It is also shown that this model can detect the same classes of objects from other virtual environments and real-world images. Further modifications to the algorithm that may be required to improve performance are discussed as future work.
ContributorsSankaramangalam Ulhas, Sangeet (Author) / Berman, Spring (Thesis advisor) / Johnson, Kathryn (Committee member) / Yong, Sze Zheng (Committee member) / Arizona State University (Publisher)
Created2019
Description
ABSTRACT
Domestic dogs have assisted humans for millennia. However, the extent to which these helpful behaviors are prosocially motivated remains unclear. To assess the propensity of pet dogs to spontaneously and actively rescue distressed humans, this study tested whether sixty pet dogs would release their seemingly trapped owners from a large box. To examine the causal mechanisms that shaped this behavior, the readiness of each dog to open the box was tested in three conditions: 1) the owner sat in the box and called for help (“Distress” test), 2) an experimenter placed high-value food rewards in the box (“Food” test), and 3) the owner sat in the box and calmly read aloud (“Reading” test).
Dogs were as likely to release their distressed owner as to retrieve treats from inside the box, indicating that rescuing an owner may be a highly rewarding action for dogs. After accounting for ability, dogs released the owner more often when the owner called for help than when the owner read aloud calmly. In addition, opening latencies decreased with test number in the Distress test but not the Reading test. Thus, rescuing the owner could not be attributed solely to social facilitation, stimulus enhancement, or social contact-seeking behavior.
Dogs displayed more stress behaviors in the Distress test than in the Reading test, and stress scores decreased with test number in the Reading test but not in the Distress test. This evidence of emotional contagion supports the hypothesis that rescuing the distressed owner was an empathetically-motivated prosocial behavior. Success in the Food task and previous (in-home) experience opening objects were both strong predictors of releasing the owner. Thus, prosocial behavior tests for dogs should control for physical ability and previous experience.
Domestic dogs have assisted humans for millennia. However, the extent to which these helpful behaviors are prosocially motivated remains unclear. To assess the propensity of pet dogs to spontaneously and actively rescue distressed humans, this study tested whether sixty pet dogs would release their seemingly trapped owners from a large box. To examine the causal mechanisms that shaped this behavior, the readiness of each dog to open the box was tested in three conditions: 1) the owner sat in the box and called for help (“Distress” test), 2) an experimenter placed high-value food rewards in the box (“Food” test), and 3) the owner sat in the box and calmly read aloud (“Reading” test).
Dogs were as likely to release their distressed owner as to retrieve treats from inside the box, indicating that rescuing an owner may be a highly rewarding action for dogs. After accounting for ability, dogs released the owner more often when the owner called for help than when the owner read aloud calmly. In addition, opening latencies decreased with test number in the Distress test but not the Reading test. Thus, rescuing the owner could not be attributed solely to social facilitation, stimulus enhancement, or social contact-seeking behavior.
Dogs displayed more stress behaviors in the Distress test than in the Reading test, and stress scores decreased with test number in the Reading test but not in the Distress test. This evidence of emotional contagion supports the hypothesis that rescuing the distressed owner was an empathetically-motivated prosocial behavior. Success in the Food task and previous (in-home) experience opening objects were both strong predictors of releasing the owner. Thus, prosocial behavior tests for dogs should control for physical ability and previous experience.
ContributorsVan Bourg, Joshua Lazar (Author) / Wynne, Clive D (Thesis advisor) / Gilby, Ian C (Committee member) / Aktipis, C. Athena (Committee member) / Arizona State University (Publisher)
Created2019
Description
Autonomous Vehicles (AVs), or self-driving cars, are poised to have an enormous impact on the automotive industry and road transportation. While advances have been made towards the development of safe, competent autonomous vehicles, there has been inadequate attention to the control of autonomous vehicles in unanticipated situations, such as imminent crashes. Even if autonomous vehicles follow all safety measures, accidents are inevitable, and humans must trust autonomous vehicles to respond appropriately in such scenarios. It is not plausible to program autonomous vehicles with a set of rules to tackle every possible crash scenario. Instead, a possible approach is to align their decision-making capabilities with the moral priorities, values, and social motivations of trustworthy human drivers.Toward this end, this thesis contributes a simulation framework for collecting, analyzing, and replicating human driving behaviors in a variety of scenarios, including imminent crashes. Four driving scenarios in an urban traffic environment were designed in the CARLA driving simulator platform, in which simulated cars can either drive autonomously or be driven by a user via a steering wheel and pedals. These included three unavoidable crash scenarios, representing classic trolley-problem ethical dilemmas, and a scenario in which a car must be driven through a school zone, in order to examine driver prioritization of reaching a destination versus ensuring safety. Sample human driving data in CARLA was logged from the simulated car’s sensors, including the LiDAR, IMU and camera. In order to reproduce human driving behaviors in a simulated vehicle, it is necessary for the AV to be able to identify objects in the environment and evaluate the volume of their bounding boxes for prediction and planning. An object detection method was used that processes LiDAR point cloud data using the PointNet neural network architecture, analyzes RGB images via transfer learning using the Xception convolutional neural network architecture, and fuses the outputs of these two networks. This method was trained and tested on both the KITTI Vision Benchmark Suite dataset and a virtual dataset exclusively generated from CARLA. When applied to the KITTI dataset, the object detection method achieved an average classification accuracy of 96.72% and an average Intersection over Union (IoU) of 0.72, where the IoU metric compares predicted bounding boxes to those used for training.
ContributorsGovada, Yashaswy (Author) / Berman, Spring (Thesis advisor) / Johnson, Kathryn (Committee member) / Marvi, Hamidreza (Committee member) / Arizona State University (Publisher)
Created2020