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Grasshoppers Regulate N: P Stoichiometric Homeostasis by Changing Phosphorus Contents in Their Frass
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Viral protein U (Vpu) is a type-III integral membrane protein encoded by Human Immunodeficiency Virus-1 (HIV- 1). It is expressed in infected host cells and plays several roles in viral progeny escape from infected cells, including down-regulation of CD4 receptors. But key structure/function questions remain regarding the mechanisms by which the Vpu protein contributes to HIV-1 pathogenesis. Here we describe expression of Vpu in bacteria, its purification and characterization. We report the successful expression of PelB-Vpu in Escherichia coli using the leader peptide pectate lyase B (PelB) from Erwinia carotovora. The protein was detergent extractable and could be isolated in a very pure form. We demonstrate that the PelB signal peptide successfully targets Vpu to the cell membranes and inserts it as a type I membrane protein. PelB-Vpu was biophysically characterized by circular dichroism and dynamic light scattering experiments and was shown to be an excellent candidate for elucidating structural models.
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This thesis conducted an evaluation of the performance and return on investment of a 2 x 6m, simple design greenhouse, as a climate control technology. Specifically, differences in internal microclimate conditions between a greenhouse treatment plot, and sun and shaded control plots were assessed and related to observed differences in crop yields across these plots. Growing conditions and productivity of two crops, tomato and swiss chard, which were grown over summer and winter growing seasons, respectively, were compared. It was found that the greenhouse was associated with improved growth conditions (as measured by the R-Index) for both crops but resulted in higher productivity only for tomatoes. Return on investment and food security impacts from the scaling of greenhouse agriculture were also explored.
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