Matching Items (277)
Description
Breast cancer is the leading cause of cancer-related deaths of women in the united states. Traditionally, Breast cancer is predominantly treated by a combination of surgery, chemotherapy, and radiation therapy. However, due to the significant negative side effects associated with these traditional treatments, there has been substantial efforts to develop alternative therapies to treat cancer. One such alternative therapy is a peptide-based therapeutic cancer vaccine. Therapeutic cancer vaccines enhance an individual's immune response to a specific tumor. They are capable of doing this through artificial activation of tumor specific CTLs (Cytotoxic T Lymphocytes). However, in order to artificially activate tumor specific CTLs, a patient must be treated with immunogenic epitopes derived from their specific cancer type. We have identified that the tumor associated antigen, TPD52, is an ideal target for a therapeutic cancer vaccine. This designation was due to the overexpression of TPD52 in a variety of different cancer types. In order to start the development of a therapeutic cancer vaccine for TPD52-related cancers, we have devised a two-step strategy. First, we plan to create a list of potential TPD52 epitopes by using epitope binding and processing prediction tools. Second, we plan to attempt to experimentally identify MHC class I TPD52 epitopes in vitro. We identified 942 potential 9 and 10 amino acid epitopes for the HLAs A1, A2, A3, A11, A24, B07, B27, B35, B44. These epitopes were predicted by using a combination of 3 binding prediction tools and 2 processing prediction tools. From these 942 potential epitopes, we selected the top 50 epitopes ranked by a combination of binding and processing scores. Due to the promiscuity of some predicted epitopes for multiple HLAs, we ordered 38 synthetic epitopes from the list of the top 50 epitope. We also performed a frequency analysis of the TPD52 protein sequence and identified 3 high volume regions of high epitope production. After the epitope predictions were completed, we proceeded to attempt to experimentally detected presented TPD52 epitopes. First, we successful transduced parental K562 cells with TPD52. After transduction, we started the optimization process for the immunoprecipitation protocol. The optimization of the immunoprecipitation protocol proved to be more difficult than originally believed and was the main reason that we were unable to progress past the transduction of the parental cells. However, we believe that we have identified the issues and will be able to complete the experiment in the coming months.
ContributorsWilson, Eric Andrew (Author) / Anderson, Karen (Thesis director) / Borges, Chad (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Visual perceptual learning (VPL) is defined as visual performance improvement after visual experiences. VPL is often highly specific for a visual feature presented during training. Such specificity is observed in behavioral tuning function changes with the highest improvement centered on the trained feature and was originally thought to be evidence for changes in the early visual system associated with VPL. However, results of neurophysiological studies have been highly controversial concerning whether the plasticity underlying VPL occurs within the visual cortex. The controversy may be partially due to the lack of observation of neural tuning function changes in multiple visual areas in association with VPL. Here using human subjects we systematically compared behavioral tuning function changes after global motion detection training with decoded tuning function changes for 8 visual areas using pattern classification analysis on functional magnetic resonance imaging (fMRI) signals. We found that the behavioral tuning function changes were extremely highly correlated to decoded tuning function changes only in V3A, which is known to be highly responsive to global motion with human subjects. We conclude that VPL of a global motion detection task involves plasticity in a specific visual cortical area.
ContributorsShibata, Kazuhisa (Author) / Chang, Li-Hung (Author) / Kim, Dongho (Author) / Nanez, Jose (Author) / Kamitani, Yukiyasu (Author) / Watanabe, Takeo (Author) / Sasaki, Yuka (Author) / New College of Interdisciplinary Arts and Sciences (Contributor)
Created2012-08-28
Description
Spasticity is a neurological disorder in which a target group of muscles remain in a contracted state. In addition to interfering with the function of these muscles, spasticity causes chronic pain and discomfort. Often found in patients with cerebral palsy, multiple sclerosis, or stroke history, spasticity affects an estimated twelve million people worldwide. Not only does spasticity cause discomfort and loss of function, but the condition can lead to contractures, or permanent shortenings of the muscle and connective tissue, if left untreated. Current treatments for spasticity are primarily different forms of muscle relaxant pharmaceuticals. Almost all of these drugs, however, carry unwanted side effects, including total muscle weakness, liver toxicity, and possible dependence. Additionally, kinesiotherapy, conducted by physical therapists at rehabilitation clinics, is often prescribed to people suffering from spasticity. Since kinesiotherapy requires frequent practice to be effective, proper treatment requires constant professional care and clinic appointments, discouraging patient compliance. Consequently, a medical device that could automate relief for spasticity outside of a clinic is desired in the market. While a number of different dynamic splints for hand spasticity are currently on the market, research has shown that these devices, which simply brace the hand in an extended position, do not work through any mechanism to decrease spastic tension over time. Two methods of temporarily reducing spasticity that have been observed in clinical studies are cryotherapy, or the decrease of temperature on a target area, and electrotherapy, which is the delivery of regulated electrical pulses to a target area. It is possible that either of these mechanisms could be incorporated into a medical device aimed toward spastic relief. In fact, electrotherapy is used in a current market device called the SaeboStim, which is advertised to help stroke recovery and spastic reduction. The purpose of this paper is to evaluate the viability of a potential spastic relief device that utilizes cryotherapy to a current and closest competitor, the SaeboStim. The effectiveness of each device in relieving spasticity is reviewed. The two devices are also compared on their ability to address primary customer needs, such as convenience, ease of use, durability, and price. Overall, it is concluded that the cryotherapy device more effectively relieves hand spasticity in users, although the SaeboStim's smaller size and better convenience gives it market appeal, and reveals some of the shortcomings in the preliminary design of the cryotherapy device.
ContributorsWiedeman, Christopher Blaise (Author) / Kleim, Jeffrey (Thesis director) / Buneo, Christopher (Committee member) / W.P. Carey School of Business (Contributor) / Harrington Bioengineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Regardless of religious beliefs, all one has left when they leave this Earth is the meaning the individual has derived from building connections and relationships with other people. I propose whether or not one is able to reach his or her potential and the quality of life that follows is based inherently in the following question: How will you manifest your truth into reality, thus becoming the best at your profession or craft, and spreading your gifts to help the lives of others? The answer to this question, and ultimately the key to leading the best life available is in the equation: vulnerability through playfulness and connection instigates happiness, which results in spreading positivity and facilitating the ability to make fearless decisions that guides one's life as an artist or profession of one's choosing, leading one to their highest potential. This theory applies to everyone and does not discriminate. This is the beginning of a life-long exploration and discovery with these elements as I further my work as an artist, and I will explore these themes from my exposure to the Balinese people and culture, our theatre workshops and experiences there, and through additional inspiration and research. The workshop and clowning in Bali training helped form the theory I have proposed, and further introduced a different approach to acting that I utilize in my own work that incorporates taksu: an achievable state of being that is unanimously understood by the Balinese that influences and shapes how they approach life, work, and each other that I discuss more extensively in my work. I will continue to inquire about how one finds space for imagination and pursuit of taksu without traveling, although traveling is an amazing way to broaden one's scope of perspective. The combination of elements discussed and taksu help create a humanity that is more in touch with inner peace and happiness as a result of spreading both positivity and a positive mindset to others. This in turn, influences and shapes performance and how we approach the craft of acting, while creating emotional truth and integrity within one's craft.
ContributorsSwim, Hannah Grace (Author) / Espinosa, Micha (Thesis director) / de la Garza, Amira (Committee member) / School of Film, Dance and Theatre (Contributor) / W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Arizona State University and Banner Thunderbird Hospital have partnered to provide pre-med students with an internship at a local emergency department. Students entering into this program will have access to each patient's vital signs, medical imaging, lab tests, and medications. This access presents students with an opportunity to learn about a variety of tools used in the assessment and treatment of emergency room patients. In order to enhance the amount of knowledge students take away from the program, I created a handbook summarizing a variety of diagnostic tests and medications. The first section of the handbook (assessment) is spilt up into the three following categories: vital signs, medical imaging, and lab tests. The second section (treatment) consists of one category, medications. Each section was written with emphasis on basic physiology, and is intended to provide pre-med students with a foundation for building further medical knowledge. Although this handbook was tailored to the information students are most likely to encounter working in Banner Thunderbird Hospital's emergency department, it is still appropriate for any student interested in learning about emergency medicine.
ContributorsBecker, Bryson (Author) / Orchinik, Miles (Thesis director) / Washo-Krupps, Delon (Committee member) / School of Life Sciences (Contributor) / W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
This creative group project aims to bring visibility to the diversity of the LGBTQ+ community at Arizona State University by sharing the personal stories and opinions of people from across ASU's rainbow spectrum. Created and produced by a gay couple that met in their first year dorm and a passionate ally, gAySU: Exploring Sparky's Rainbow is a compilation of photos, videos, and stories from ASU students, faculty, and staff that identify with the LGBTQ+ community. When reflecting on their own journey over the past three years, gAySU's creators recognized ASU can feel large with many pockets of communities, yet through trial and error over the past 4 years they managed to explore their identities and grow as individuals, as a couple, and as an ally. By sharing their story and the stories of others, it is the hope that gAySU allows its readers and viewers an insight into what it means to be both a Sun Devil and LGBTQ+, and for those new to or anxious to identify with the LGBTQ+ community, to understand they are not alone in their journey and that it is okay to bleed maroon, gold, and all other colors of the rainbow.
ContributorsKing, Shay Scott (Co-author) / Hendricks, Brock (Co-author) / Hom, Claire (Co-author) / Dove-Viebahn, Aviva (Thesis director) / Sanchez, Daniel (Committee member) / W.P. Carey School of Business (Contributor) / School of Politics and Global Studies (Contributor) / Department of Supply Chain Management (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
This thesis explores the various reasons that Broadway musicals fail, or "flop". It goes into detail on several musicals of different genres, and explains what their shortcomings were, whether it be a less-than-perfect book, a weak score, or a production team that was not experienced in the skills that it takes to put together a musical. It discusses how long the shows ran for, or if they ever made it into a Broadway house. It looks at what the audience reception was like for each show, and whether the shows were a success with the tough critics of Broadway. In addition to this, I have recorded several of my peers performing songs from the musicals I have discussed in the written portion of this thesis. This helps to educate an audience on what it would have been like for one of the flop's real performances. The videos also do a wonderful job of communicating what the show sounds like, and how it can make an audience feel or react. Lastly, it gives more insight into why the musicals they are from failed to thrive in front of a Broadway audience. Through my research, I have discovered that creating a musical is possibly one of the most difficult feats a team of creative people can accomplish. There are countless reasons for failure in these shows, but overall, I feel that every failure had something to offer, and because of that, are surely worth celebrating.
ContributorsUrias-Ramonett, Sedona Lee (Author) / Yatso, Toby (Thesis director) / Dreyfoos, Dale (Committee member) / W.P. Carey School of Business (Contributor) / School of Music (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05