Matching Items (129)
Description
Continuous monitoring in the adequate temporal and spatial scale is necessary for a better understanding of environmental variations. But field deployments of molecular biological analysis platforms in that scale are currently hindered because of issues with power, throughput and automation. Currently, such analysis is performed by the collection of large sample volumes from over a wide area and transporting them to laboratory testing facilities, which fail to provide any real-time information. This dissertation evaluates the systems currently utilized for in-situ field analyses and the issues hampering the successful deployment of such bioanalytial instruments for environmental applications. The design and development of high throughput, low power, and autonomous Polymerase Chain Reaction (PCR) instruments, amenable for portable field operations capable of providing quantitative results is presented here as part of this dissertation. A number of novel innovations have been reported here as part of this work in microfluidic design, PCR thermocycler design, optical design and systems integration. Emulsion microfluidics in conjunction with fluorinated oils and Teflon tubing have been used for the fluidic module that reduces cross-contamination eliminating the need for disposable components or constant cleaning. A cylindrical heater has been designed with the tubing wrapped around fixed temperature zones enabling continuous operation. Fluorescence excitation and detection have been achieved by using a light emitting diode (LED) as the excitation source and a photomultiplier tube (PMT) as the detector. Real-time quantitative PCR results were obtained by using multi-channel fluorescence excitation and detection using LED, optical fibers and a 64-channel multi-anode PMT for measuring continuous real-time fluorescence. The instrument was evaluated by comparing the results obtained with those obtained from a commercial instrument and found to be comparable. To further improve the design and enhance its field portability, this dissertation also presents a framework for the instrumentation necessary for a portable digital PCR platform to achieve higher throughputs with lower power. Both systems were designed such that it can easily couple with any upstream platform capable of providing nucleic acid for analysis using standard fluidic connections. Consequently, these instruments can be used not only in environmental applications, but portable diagnostics applications as well.
ContributorsRay, Tathagata (Author) / Youngbull, Cody (Thesis advisor) / Goryll, Michael (Thesis advisor) / Blain Christen, Jennifer (Committee member) / Yu, Hongyu (Committee member) / Arizona State University (Publisher)
Created2013
Description
Global Positioning System (GPS) is a navigation system widely used in civilian and military application, but its accuracy is highly impacted with consequential fading, and possible loss of communication due to multipath propagation and high power interferences. This dissertation proposes alternatives to improve the performance of the GPS receivers to obtain a system that can be reliable in critical situations. The basic performance of the GPS receiver consists of receiving the signal with an antenna array, delaying the signal at each antenna element, weighting the delayed replicas, and finally, combining the weighted replicas to estimate the desired signal. Based on these, three modifications are proposed to improve the performance of the system. The first proposed modification is the use of the Least Mean Squares (LMS) algorithm with two variations to decrease the convergence time of the classic LMS while achieving good system stability. The results obtained by the proposed LMS demonstrate that the algorithm can achieve the same stability as the classic LMS using a small step size, and its convergence rate is better than the classic LMS using a large step size. The second proposed modification is to replace the uniform distribution of the time delays (or taps) by an exponential distribution that decreases the bit-error rate (BER) of the system without impacting the computational efficiency of the uniform taps. The results show that, for a BER of 0.001, the system can operate with a 1 to 2 dB lower signal-to-noise ratio (SNR) when an exponential distribution is used rather than a uniform distribution. Finally, the third modification is implemented in the design of the antenna array. In this case, the gain of each microstrip element is enhanced by embedding ferrite rings in the substrate, creating a hybrid substrate. The ferrite rings generates constructive interference between the incident and reflected fields; consequently, the gain of a single microstrip element is enhanced by up to 4 dB. When hybrid substrates are used in microstrip element arrays, a significant enhancement in angle range is achieved for a given reflection coefficient compared to using a conventional substrate.
ContributorsRivera-Albino, Alix (Author) / Balanis, Constantine A (Thesis advisor) / Tepedelenlioğlu, Cihan (Committee member) / Kiaei, Sayfe (Committee member) / Aberle, James T (Committee member) / Arizona State University (Publisher)
Created2013
Description
Surface plasmon resonance (SPR) has emerged as a popular technique for elucidating subtle signals from biological events in a label-free, high throughput environment. The efficacy of conventional SPR sensors, whose signals are mass-sensitive, diminishes rapidly with the size of the observed target molecules. The following work advances the current SPR sensor paradigm for the purpose of small molecule detection. The detection limits of two orthogonal components of SPR measurement are targeted: speed and sensitivity. In the context of this report, speed refers to the dynamic range of measured kinetic rate constants, while sensitivity refers to the target molecule mass limitation of conventional SPR measurement. A simple device for high-speed microfluidic delivery of liquid samples to a sensor surface is presented to address the temporal limitations of conventional SPR measurement. The time scale of buffer/sample switching is on the order of milliseconds, thereby minimizing the opportunity for sample plug dispersion. The high rates of mass transport to and from the central microfluidic sensing region allow for SPR-based kinetic analysis of binding events with dissociation rate constants (kd) up to 130 s-1. The required sample volume is only 1 μL, allowing for minimal sample consumption during high-speed kinetic binding measurement. Charge-based detection of small molecules is demonstrated by plasmonic-based electrochemical impedance microscopy (P-EIM). The dependence of surface plasmon resonance (SPR) on surface charge density is used to detect small molecules (60-120 Da) printed on a dextran-modified sensor surface. The SPR response to an applied ac potential is a function of the surface charge density. This optical signal is comprised of a dc and an ac component, and is measured with high spatial resolution. The amplitude and phase of local surface impedance is provided by the ac component. The phase signal of the small molecules is a function of their charge status, which is manipulated by the pH of a solution. This technique is used to detect and distinguish small molecules based on their charge status, thereby circumventing the mass limitation (~100 Da) of conventional SPR measurement.
ContributorsMacGriff, Christopher Assiff (Author) / Tao, Nongjian (Thesis advisor) / Wang, Shaopeng (Committee member) / LaBaer, Joshua (Committee member) / Chae, Junseok (Committee member) / Arizona State University (Publisher)
Created2013
Description
"Sensor Decade" has been labeled on the first decade of the 21st century. Similar to the revolution of micro-computer in 1980s, sensor R&D; developed rapidly during the past 20 years. Hard workings were mainly made to minimize the size of devices with optimal the performance. Efforts to develop the small size devices are mainly concentrated around Micro-electro-mechanical-system (MEMS) technology. MEMS accelerometers are widely published and used in consumer electronics, such as smart phones, gaming consoles, anti-shake camera and vibration detectors. This study represents liquid-state low frequency micro-accelerometer based on molecular electronic transducer (MET), in which inertial mass is not the only but also the conversion of mechanical movement to electric current signal is the main utilization of the ionic liquid. With silicon-based planar micro-fabrication, the device uses a sub-micron liter electrolyte droplet sealed in oil as the sensing body and a MET electrode arrangement which is the anode-cathode-cathode-anode (ACCA) in parallel as the read-out sensing part. In order to sensing the movement of ionic liquid, an imposed electric potential was applied between the anode and the cathode. The electrode reaction, I_3^-+2e^___3I^-, occurs around the cathode which is reverse at the anodes. Obviously, the current magnitude varies with the concentration of ionic liquid, which will be effected by the movement of liquid droplet as the inertial mass. With such structure, the promising performance of the MET device design is to achieve 10.8 V/G (G=9.81 m/s^2) sensitivity at 20 Hz with the bandwidth from 1 Hz to 50 Hz, and a low noise floor of 100 ug/sqrt(Hz) at 20 Hz.
ContributorsLiang, Mengbing (Author) / Yu, Hongyu (Thesis advisor) / Jiang, Hanqing (Committee member) / Kozicki, Micheal (Committee member) / Arizona State University (Publisher)
Created2013
Description
This dissertation proposes a miniature FIR filter that works at microwave frequencies, whose filter response can ideally be digitally programmed. Such a frequency agile device can find applications in cellular communications and wireless networking. The basic concept of the FIR filter utilizes a low loss acoustic waveguide of appropriate geometry that acts as a traveling wave tapped-delay line. The input RF signal is applied by an array of capacitive transducers at various locations on the acoustic waveguide at one end that excites waves of a propagating acoustic mode with varying spatial delays and amplitudes which interfere as they propagate. The output RF signal is picked up at the other end of the waveguide by another array of capacitive transducers. Tuning of the FIR filter coefficients is realized by controlling the DC voltage profile applied to the individual transducers which essentially shapes the overall filter response. Equivalent circuit modeling of the capacitive transducer, acoustic waveguide and transducer-line coupling is presented in this dissertation. A theoretical model for the filter is developed from a general theory of an array of transducers exciting a waveguide and is used to obtain a set of filter design equations. A MATLAB based circuit simulator is developed to simulate the filter responses. Design parameters and simulation results obtained for an example waveguide structure are presented and compared to the values estimated by the theoretical model. A waveguide structure utilizing the Rayleigh-like mode of a ridge is then introduced. A semi-analytical method to obtain propagating elastic modes of such a ridge waveguide etched in an anisotropic crystal is presented. Microfabrication of a filter based on ridges etched in single crystal Silicon is discussed along with details of the challenges faced. Finally, future work and a few alternative designs are presented that can have a better chance of success. Analysis and modeling work to this point has given a good understanding of the working principles, performance tradeoffs and fabrication pitfalls of the proposed device. With the appropriate acoustic waveguide structure, the proposed device could make it possible to realize miniature programmable FIR filters in the GHz range.
ContributorsGalinde, Ameya (Author) / Abbaspour-Tamijani, Abbas (Thesis advisor) / Chae, Junseok (Committee member) / Pan, George (Committee member) / Phillips, Stephen (Committee member) / Arizona State University (Publisher)
Created2013
Description
Under the framework of intelligent management of power grids by leveraging advanced information, communication and control technologies, a primary objective of this study is to develop novel data mining and data processing schemes for several critical applications that can enhance the reliability of power systems. Specifically, this study is broadly organized into the following two parts: I) spatio-temporal wind power analysis for wind generation forecast and integration, and II) data mining and information fusion of synchrophasor measurements toward secure power grids. Part I is centered around wind power generation forecast and integration. First, a spatio-temporal analysis approach for short-term wind farm generation forecasting is proposed. Specifically, using extensive measurement data from an actual wind farm, the probability distribution and the level crossing rate of wind farm generation are characterized using tools from graphical learning and time-series analysis. Built on these spatial and temporal characterizations, finite state Markov chain models are developed, and a point forecast of wind farm generation is derived using the Markov chains. Then, multi-timescale scheduling and dispatch with stochastic wind generation and opportunistic demand response is investigated. Part II focuses on incorporating the emerging synchrophasor technology into the security assessment and the post-disturbance fault diagnosis of power systems. First, a data-mining framework is developed for on-line dynamic security assessment by using adaptive ensemble decision tree learning of real-time synchrophasor measurements. Under this framework, novel on-line dynamic security assessment schemes are devised, aiming to handle various factors (including variations of operating conditions, forced system topology change, and loss of critical synchrophasor measurements) that can have significant impact on the performance of conventional data-mining based on-line DSA schemes. Then, in the context of post-disturbance analysis, fault detection and localization of line outage is investigated using a dependency graph approach. It is shown that a dependency graph for voltage phase angles can be built according to the interconnection structure of power system, and line outage events can be detected and localized through networked data fusion of the synchrophasor measurements collected from multiple locations of power grids. Along a more practical avenue, a decentralized networked data fusion scheme is proposed for efficient fault detection and localization.
ContributorsHe, Miao (Author) / Zhang, Junshan (Thesis advisor) / Vittal, Vijay (Thesis advisor) / Hedman, Kory (Committee member) / Si, Jennie (Committee member) / Ye, Jieping (Committee member) / Arizona State University (Publisher)
Created2013
Description
The fluorescence enhancement by a single Noble metal sphere is separated into excitation/absorption enhancement and the emission quantum yield enhancement. Incorporating the classical model of molecular spontaneous emission into the excitation/absorption transition, the excitation enhancement is calculated rigorously by electrodynamics in the frequency domain. The final formula for the excitation enhancement contains two parts: the primary field enhancement calculated from the Mie theory, and a derating factor due to the backscattering field from the molecule. When compared against a simplified model that only involves the primary Mie theory field calculation, this more rigorous model indicates that the excitation enhancement near the surface of the sphere is quenched severely due to the back-scattering field from the molecule. The degree of quenching depends in part on the bandwidth of the illumination because the presence of the sphere induces a red-shift in the absorption frequency of the molecule and at the same time broadens its spectrum. Monochromatic narrow band illumination at the molecule's original (unperturbed) resonant frequency yields large quenching. For the more realistic broadband illumination scenario, we calculate the final enhancement by integrating over the excitation/absorption spectrum. The numerical results indicate that the resonant illumination scenario overestimates the quenching and therefore would underestimate the total excitation enhancement if the illumination has a broader bandwidth than the molecule. Combining the excitation model with the exact Electrodynamical theory for emission, the complete realistic model demonstrates that there is a potential for significant fluorescence enhancement only for the case of a low quantum yield molecule close to the surface of the sphere. General expressions of the fluorescence enhancement for arbitrarily-shaped metal antennas are derived. The finite difference time domain method is utilized for analyzing these complicated antenna structures. We calculate the total excitation enhancement for the two-sphere dimer. Although the enhancement is greater in this case than for the single sphere, because of the derating effects the total enhancement can never reach the local field enhancement. In general, placing molecules very close to a plasmonic antenna surface yields poor enhancement because the local field is strongly affected by the molecular self-interaction with the metal antenna.
ContributorsZhang, Zhe (Author) / Diaz, Rodolfo E (Thesis advisor) / Lim, Derrick (Thesis advisor) / Pan, George (Committee member) / Yu, Hongyu (Committee member) / Arizona State University (Publisher)
Created2013
Description
With the rapid growth of power systems and the concomitant technological advancements, the goal of achieving smart grids is no longer a vision but a foreseeable reality. Hence, the existing grids are undergoing infrastructural modifications to achieve the diverse characteristics of a smart grid. While there are many subjects associated with the operation of smart grids, this dissertation addresses two important aspects of smart grids: increased penetration of renewable resources, and increased reliance on sensor systems to improve reliability and performance of critical power system components. Present renewable portfolio standards are changing both structural and performance characteristics of power systems by replacing conventional generation with alternate energy resources such as photovoltaic (PV) systems. The present study investigates the impact of increased penetration of PV systems on steady state performance as well as transient stability of a large power system which is a portion of the Western U.S. interconnection. Utility scale and residential rooftop PVs are added to replace a portion of conventional generation resources. While steady state voltages are observed under various PV penetration levels, the impact of reduced inertia on transient stability performance is also examined. The simulation results obtained effectively identify both detrimental and beneficial impacts of increased PV penetration both for steady state stability and transient stability performance. With increased penetration of the renewable energy resources, and with the current loading scenario, more transmission system components such as transformers and circuit breakers are subject to increased stress and overloading. This research work explores the feasibility of increasing system reliability by applying condition monitoring systems to selected circuit breakers and transformers. A very important feature of smart grid technology is that this philosophy decreases maintenance costs by deploying condition monitoring systems that inform the operator of impending failures; or the approach can ameliorate problematic conditions. A method to identify the most critical transformers and circuit breakers with the aid of contingency ranking methods is presented in this study. The work reported in this dissertation parallels an industry sponsored study in which a considerable level of industry input and industry reported concerns are reflected.
ContributorsEftekharnejad, Sara (Author) / Heydt, Gerald (Thesis advisor) / Vittal, Vijay (Thesis advisor) / Si, Jennie (Committee member) / Tylavsky, Daniel (Committee member) / Arizona State University (Publisher)
Created2012
Description
Asymptotic and Numerical methods are popular in applied electromagnetism. In this work, the two methods are applied for collimated antennas and calibration targets, respectively. As an asymptotic method, the diffracted Gaussian beam approach (DGBA) is developed for design and simulation of collimated multi-reflector antenna systems, based upon Huygens principle and independent Gaussian beam expansion, referred to as the frames. To simulate a reflector antenna in hundreds to thousands of wavelength, it requires 1E7 - 1E9 independent Gaussian beams. To this end, high performance parallel computing is implemented, based on Message Passing Interface (MPI). The second part of the dissertation includes the plane wave scattering from a target consisting of doubly periodic array of sharp conducting circular cones by the magnetic field integral equation (MFIE) via Coiflet based Galerkin's procedure in conjunction with the Floquet theorem. Owing to the orthogonally, compact support, continuity and smoothness of the Coiflets, well-conditioned impedance matrices are obtained. Majority of the matrix entries are obtained in the spectral domain by one-point quadrature with high precision. For the oscillatory entries, spatial domain computation is applied, bypassing the slow convergence of the spectral summation of the non-damping propagating modes. The simulation results are compared with the solutions from an RWG-MLFMA based commercial software, FEKO, and excellent agreement is observed.
ContributorsWang, Le, 1975- (Author) / Pan, George (Thesis advisor) / Yu, Hongyu (Committee member) / Aberle, James T., 1961- (Committee member) / Diaz, Rodolfo (Committee member) / Kitchen, Jennifer (Committee member) / Arizona State University (Publisher)
Created2012
Description
Harsh environments have conditions that make collecting scientific data difficult with existing commercial-off-the-shelf technology. Micro Electro Mechanical Systems (MEMS) technology is ideally suited for harsh environment characterization and operation due to the wide range of materials available and an incredible array of different sensing techniques while providing small device size, low power consumption, and robustness. There were two main objectives of the research conducted. The first objective was to design, fabricate, and test novel sensors that measure the amount of exposure to ionizing radiation for a wide range of applications including characterization of harsh environments. Two types of MEMS ionizing radiation dosimeters were developed. The first sensor was a passive radiation-sensitive capacitor-antenna design. The antenna's emitted frequency of peak-intensity changed as exposure time to radiation increased. The second sensor was a film bulk acoustic-wave resonator, whose resonant frequency decreased with increasing ionizing radiation exposure time. The second objective was to develop MEMS sensor systems that could be deployed to gather scientific data and to use that data to address the following research question: do temperature and/or conductivity predict the appearance of photosynthetic organisms in hot springs. To this end, temperature and electrical conductivity sensor arrays were designed and fabricated based on mature MEMS technology. Electronic circuits and the software interface to the electronics were developed for field data collection. The sensor arrays utilized in the hot springs yielded results that support the hypothesis that temperature plays a key role in determining where the photosynthetic organisms occur. Additionally, a cold-film fluidic flow sensor was developed, which is suitable for near-boiling temperature measurement. Future research should focus on (1) developing a MEMS pH sensor array with integrated temperature, conductivity, and flow sensors to provide multi-dimensional data for scientific study and (2) finding solutions to biofouling and self-calibration, which affects sensor performance over long-term deployment.
ContributorsOiler, Jonathon (Author) / Yu, Hongyu (Thesis advisor) / Anbar, Ariel (Committee member) / Hartnett, Hilairy (Committee member) / Scannapieco, Evan (Committee member) / Timmes, Francis (Committee member) / Arizona State University (Publisher)
Created2013