Matching Items (327)
Description
Colorectal cancer (CRC) is one of the most highly diagnosed cancers in the United States and accounts for 9.5% of all new cancer cases worldwide. With a 50% five-year prognosis, it is the second highest cancerous cause of death in the U.S. CRC tumors express antigens that are capable of inducing an immune response. The identification of autoantibodies (AAb) against tumor-associated antigens (TAA) may facilitate personalized tumor treatment in the form of targeted immunotherapy. The objective of this study was to observe the AAb expression raised against a 2000 human gene survey in late-stage colorectal cancer using the Nucleic Acid Programmable Protein Arrays (NAPPA). AAbs from serum samples were collected from 80 patients who died within 24 months of their last blood draw and 80 age and gender matched healthy control were profiled using NAPPA. TAA p53, a well-established protein that is one of the most highly mutated across a variety of cancers, was one of the top candidates based on statistical analysis, which, along with its family proteins p63 and p73 (which showed inverse AAb response profiles) warranted further testing via RAPID ELISA. Statistical analysis from these results revealed an inverse differential relationship between p53 and p63, in which p53 seropositivity was higher in patients than in controls, while the opposite was unexpectedly the case for p63. This study involving the AAb immunoprofiling of advanced stage CRC patients is one of the first to shed light on the high-throughput feasibility of immunoproteomic experiments using protein arrays as well as the identification of immunotherapy targets in a more rapid move towards specialized treatment of advanced CRC.
ContributorsSzeto, Emily (Author) / LaBaer, Joshua (Thesis director) / Qiu, Ji (Committee member) / Demirkan, Gokhan (Committee member) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor)
Created2014-12
Description
While public transit systems are perceived to produce lower GHG emission intensities per passenger miles traveled (PMT) and per vehicle miles traveled (VMT), there is a limited understanding of emissions per PMT/VMT across cities, or of how emissions may change across modes (light, metro, commuter, and bus) and time (e.g., with changing electricity mixes in the future). In order to better understand the GHG emissions intensity of public transit systems, a comparative emissions assessment was developed utilizing the National Transit Database (NTD) which reports energy use from 1997 to 2012 of rail and bus systems across the US. By determining the GHG emission intensities (per VMT or per PMT) for each mode of transit across multiple years, the modes of transit can be better compared between one another. This comparison can help inform future goals to reduce GHG emissions as well as target reductions from the mode of transit that has the highest emissions. The proposed analysis of the NTD and comparison of modal emission intensities will be used to develop future forecasting that can guide public transit systems towards a sustainable future.
ContributorsCano, Alex (Author) / Chester, Mikhail (Thesis director) / Seager, Thomas (Committee member) / Civil, Environmental and Sustainable Engineering Programs (Contributor) / Barrett, The Honors College (Contributor) / School of Sustainability (Contributor) / School of Human Evolution and Social Change (Contributor)
Created2014-12
Description
I began this thesis with one goal in mind: to discover which career path is best for me. The plan was to take a critical look at my life using deductive logic and Information Measurement Theory (IMT) to determine who I am and through that knowledge ultimately align my future with where I would be best suited career-wise. I was interested in this because I was fascinated with IMT and I wanted to apply it to something that I would find useful. I knew that I would be graduating and I also knew that I had no idea what I wanted the rest of my life to look like. For me, it was like killing two birds with one stone: delving deeper into a fascinating theory as well as determining what to do with my future. I began my project by analyzing my own life and creating a timeline of major events in my life. Then, I pieced together the characteristics of my family to gain perspective of their lives and how their lives have influenced mine. As I explored this information and the implications this knowledge had in my life, my thesis began to take a new direction. The more I began to understand myself, the more I realized that I needed to spend more time on this area of my project. I realized the importance of understanding who I am at the core in order to be the best person that I can be. To reach happiness, be fulfilled, be a good person, I needed to spend time understanding myself rather than just jumping forward into my future.
ContributorsCrepeau, Megan Grace (Author) / Kashiwagi, Dean (Thesis director) / Kashiwagi, Jacob (Committee member) / Barrett, The Honors College (Contributor) / School of Human Evolution and Social Change (Contributor)
Created2015-05
Description
This project explores a variety of ways of framing the problem of obesity, beginning with a multidisciplinary assessment of genetic, environmental, cultural, nutritional, and socioeconomic factors involved in the structure and the consequences of each frame. How obesity is framed as a problem has a profound impact on the kinds of solutions that may be deemed scientifically appropriate. But frames are not entirely evidence-based, inasmuch as political and moral values infuse debates about the nature of obesity. Drawing on interdisciplinary resources from bioethics and the philosophy of science, I strive to offer strategic insight in to how to navigate the complexity of these issues.
ContributorsYanamandra, Meghana (Author) / Robert, Jason (Thesis director) / Wharton, Christopher (Committee member) / Drago, Mary (Committee member) / Barrett, The Honors College (Contributor) / School of Sustainability (Contributor) / School of Life Sciences (Contributor) / School of Human Evolution and Social Change (Contributor)
Created2014-05
Description
The interview is a vital part of the reporter's story. In a reporter's quest for the truth, the interview can give the reporter an understanding of the source, a sense of what the source represents to the story and get the source to disclose enough information to properly tell the story. In this paper, the key body language of reporters and sources during the journalistic interview are evaluated. To help the reporter get the most out of an interview, this study observes key behaviors from reporters and sources of five major areas of kinesics: hand gesture, posture, proxemics, mirroring and facial expressions. This study categorized the positive from the negative gestures associated with these major movements in order to help evaluate the attitude of a interview participant and how to best improve relations through a reporter's subtle body gestures.
ContributorsMykleseth, Kathryn Jo (Author) / Thornton, Leslie (Thesis director) / Rueles, Richard (Committee member) / Barrett, The Honors College (Contributor) / Walter Cronkite School of Journalism and Mass Communication (Contributor) / School of Human Evolution and Social Change (Contributor)
Created2014-05
Description
Because children do not have the same decision-making powers as adults in matters affecting their health, their opinions have often been underrepresented in research (Bradding & Horstman, 1999). However, there is growing interest in the way that children view health because this knowledge elicits the development of more child-centered and effective approaches to health education and intervention (Bradding & Horstman, 1999). Professionals have often utilized the write-and-draw technique in school settings to gain a better understanding of how to best implement health education programs. The "bottom-up" approach of the write-and-draw method encourages participation and has been shown to elicit thoughtful responses about how children conceptualize health (Pridmore & Bendelow, 1995). This study uses the write-and-draw method to perform a cross- cultural comparison of child perspectives of health in the United States and Guatemala, countries that represent contrasting paradigms for child health. The results of this study are consistent with previous research, especially the emergent health themes. Children from the United States and Guatemala predominantly depicted health in terms of food. Guatemalan students were more likely to refer to hygienic practices and environmental conditions, while US children mentioned vegetables, water, and exercise as being healthy. For the unhealthy category, themes of poor hygiene, chips, fat/grease, fruit, carbohydrates, and environment were mentioned more often in Guatemala, while U.S. students listed sweets and fast food more frequently. Results support claims made in other literature that children's concepts of health are shaped by life experience and social context. Potential applications of the research include exposing areas (themes) where children are less likely to understand health implications and developing educational curriculum to increase a more comprehensive understanding of health.
ContributorsRenslow, Jillian Marie (Author) / Maupin, Jonathan (Thesis director) / BurnSilver, Shauna (Committee member) / Barrett, The Honors College (Contributor) / School of Human Evolution and Social Change (Contributor) / School of International Letters and Cultures (Contributor)
Created2015-05
Description
This project investigates how experiences colleges create for admitted students impact students' excitement for, satisfaction with, and likelihood to attend the college, analyzed by different subgroups, and how non-yielded students compare their college selection to W. P. Carey on various metrics. This study found that top admit students were less likely to attend, less satisfied, and less excited with the services offered than their counterparts and recommendations were made to improve the gap.
ContributorsGullo, Kelley (Co-author) / Dwosh, Bennett (Co-author) / Ostrom, Amy (Thesis director) / Olsen, Douglas (Committee member) / Desch, Timothy (Committee member) / Barrett, The Honors College (Contributor) / Department of Economics (Contributor) / Department of Marketing (Contributor) / School of Human Evolution and Social Change (Contributor) / Department of Management (Contributor) / W. P. Carey School of Business (Contributor)
Created2015-05
Description
The focus of this project was to look at alternative treatments for endocrine resistant breast cancer (ERBC), which are breast cancers that have become resistant to hormone therapies such as Tamoxifen or aromatase inhibitors. The first part of this project involves investigating the relationship between histone de-acetylase inhibitor Vorinostat and Tamoxifen in MCF7 G11 cells, Tamoxifen resistant sub-clones, according to the PSOC Time grant. The second part involves targeting the androgen receptor (AR) in MCF7 sub-clones with AR antagonists, Bicalutamide and MDV3100, and investigating the possible usage of AR as a biomarker, due to over-expression of AR in ERBC, in accordance with the Mayo ASU Seed Grant.
The synergistic effects between Vorinostat and Tamoxifen observed through a phase II study on breast cancer patients resistant to hormone therapy may involve more than the modulation of ER-alpha to reverse Tamoxifen resistance in ERBC cells. RT-qPCR of genes expressed in Tamoxifen resistant cells, trefoil factor 1(TFF1) and v-myc avian myelocytomatosis viral oncogene homolog (MYC), were evaluated along with ESR1 and Diablo as a control. MYC was observed to have increased expression in the treated cells, whereas the other genes had a decrease in their expression levels after the cells were treated for 3 days with Vorinostat IC30 of 1 µM. As for targeting the AR, MCF7 Tamoxifen sensitive and resistant cells were not affected by the AR antagonists to determine an IC50. The cell viability for all MCF7 sub-clones only decreased for high concentrations of 5.56 µM - 50 µM in Bicalutamide and 16.67 µM – 50 µM of MDV1300. Furthermore, hormone depletion of MCF7 G11 Tamoxifen resistant sub-clones did not show a great response to DHT stimulation or the AR antagonists. In the RT-qPCR, the MCF7 G11 cells showed an increase in mRNA expression for ER, AR, and PR after 4 hours of treatment with estradiol. As for the DHT treatment, ER, AR, PR, and PSA had a minimal increase in the fold change, but the fold change in AR was less than in the estradiol treatment. The Mayo Clinic will investigate the possible usage of AR as a biomarker through immunohistochemistry.
The synergistic effects between Vorinostat and Tamoxifen observed through a phase II study on breast cancer patients resistant to hormone therapy may involve more than the modulation of ER-alpha to reverse Tamoxifen resistance in ERBC cells. RT-qPCR of genes expressed in Tamoxifen resistant cells, trefoil factor 1(TFF1) and v-myc avian myelocytomatosis viral oncogene homolog (MYC), were evaluated along with ESR1 and Diablo as a control. MYC was observed to have increased expression in the treated cells, whereas the other genes had a decrease in their expression levels after the cells were treated for 3 days with Vorinostat IC30 of 1 µM. As for targeting the AR, MCF7 Tamoxifen sensitive and resistant cells were not affected by the AR antagonists to determine an IC50. The cell viability for all MCF7 sub-clones only decreased for high concentrations of 5.56 µM - 50 µM in Bicalutamide and 16.67 µM – 50 µM of MDV1300. Furthermore, hormone depletion of MCF7 G11 Tamoxifen resistant sub-clones did not show a great response to DHT stimulation or the AR antagonists. In the RT-qPCR, the MCF7 G11 cells showed an increase in mRNA expression for ER, AR, and PR after 4 hours of treatment with estradiol. As for the DHT treatment, ER, AR, PR, and PSA had a minimal increase in the fold change, but the fold change in AR was less than in the estradiol treatment. The Mayo Clinic will investigate the possible usage of AR as a biomarker through immunohistochemistry.
ContributorsVorachitti, Merica (Author) / LaBaer, Joshua (Thesis director) / Anderson, Karen (Committee member) / Gonzalez, Laura (Committee member) / Barrett, The Honors College (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2014-05
Description
This paper will explore the themes of performing race in hip-hop music. Through the genesis of the hip-hop industry in the Bronx in the 1970s, rap music has become aligned with afrocentric themes. The music spoke to inner cities and often held themes of economic abandonment, racism, and poverty. Today, non-black hip-hop artists are entering the rap scene. However, the mainstream hip-hop industry is still dominated by black artists. We explore the idea of performing race, specifically performing blackness. Non-black artists do this by dressing a certain way, speaking a certain way, and moving a certain way. We have chosen to identify three case studies to help us explore these ideas and understand how race is still important in the hip-hop industry today. Our case studies include Mellow Man Ace, Jin, and Brother Ali. While success is a complex term, we have discovered that race still follows a predictive outcome in monetary success and fame. The hip-hop industry is always in dynamic change. Our paper attempts to open the dialogue for talking about race and hip-hop music.
ContributorsKoyama, Curtis (Co-author) / Chiu, Carissa (Co-author) / Bhattacharjya, Nilanjana (Thesis director) / Montesano, Mark (Committee member) / Barrett, The Honors College (Contributor) / Department of Marketing (Contributor) / W. P. Carey School of Business (Contributor) / School of Human Evolution and Social Change (Contributor)
Created2014-05
Description
This creative project attempts to capture the fervor of bringing an immersive attraction from page to park by providing the different points of view of several fictitious teams—such as engineers, marketing experts, and set designers—which are involved in the creation and maintenance of a theoretical theme park ride. The specific theme park ride detailed below, tentatively titled The Once and Future Dark Ride, stands as an allegory for the majority of “dark rides” in the theme park world. Different points of view are detailed in order to give readers an approximation of the ride design process.
ContributorsMatsen, Julie Andrea (Author) / Facinelli, Diane (Thesis director) / Foy, Joseph (Committee member) / Barrett, The Honors College (Contributor) / School of Human Evolution and Social Change (Contributor) / Department of English (Contributor)
Created2014-05