Matching Items (320)
Description
Protein AMPylation is a recently discovered and relatively unstudied post-translational modification (PTM). AMPylation has previously been shown to play an important role in metabolic regulation and host pathogenesis in bacteria, but the recent identification of potential AMPylators across many species in every domain of life has supported the possibility that AMPylation could be a more fundamental and physiologically significant regulatory PTM. For the first time, we characterized the auto-AMPylation capability of the human protein SOS1 through in vitro AMPylation experiments using full-length protein and whole-domain truncation mutants. We found that SOS1 can become AMPylated at a tyrosine residue possibly within the Cdc25 domain of the protein, the Dbl homology domain is vital for efficient auto-AMPylation activity, and the C-terminal proline-rich domain exhibits a complex regulatory function. The proline-rich domain alone also appears to be capable of catalyzing a separate, unidentified covalent self-modification using a fluorescent ATP analogue. Finally, SOS1 was shown to be capable of catalyzing the AMPylation of two endogenous human protein substrates: a ubiquitous, unidentified protein of ~49kDa and another breast-cancer specific, unidentified protein of ~28kDa.
ContributorsOber-Reynolds, Benjamin John (Author) / LaBaer, Joshua (Thesis director) / Borges, Chad (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor) / School of Life Sciences (Contributor)
Created2014-05
Description
Abstract
Objective: The purpose of this literature review is to examine the most psychometrically sound (e.g., valid and reliable) instruments measuring physical activity (PA) so that reflection of preschool children’s activity and documentation of intervention effects on preschool child PA is accurate.
Methods: Rigorous validation and calibration studies and those studies designed to test the psychometric properties of PA measurement instruments were specifically sought out to include in this review. Articles were excluded if they did not include specific information about the validity and reliability of the PA measures used with preschool children.
Discussion: Of the six articles reviewed, the systematic review used the most rigorous protocols to conduct its review, yielding the highest level of evidence appraising several validation studies. Because Pfeiffer et al. (2006) utilized the most valid and reliable criterion measure (Cosmed® portable metabolic system), the validation study this research team conducted is deemed to have identified the most valid and reliable cut points to utilize when reducing accelerometry data.
Conclusion: Current cut-points vary widely and greatly affect the reported results of a study. Therefore, it is critical that authors reference validation studies used to support the cut-points that were employed in the data-reduction phase. Currently, validation methods have been identified using high quality criterion measures in rigorous validation studies and thus it is strongly recommended that those cut points be used in data reduction processes.
Objective: The purpose of this literature review is to examine the most psychometrically sound (e.g., valid and reliable) instruments measuring physical activity (PA) so that reflection of preschool children’s activity and documentation of intervention effects on preschool child PA is accurate.
Methods: Rigorous validation and calibration studies and those studies designed to test the psychometric properties of PA measurement instruments were specifically sought out to include in this review. Articles were excluded if they did not include specific information about the validity and reliability of the PA measures used with preschool children.
Discussion: Of the six articles reviewed, the systematic review used the most rigorous protocols to conduct its review, yielding the highest level of evidence appraising several validation studies. Because Pfeiffer et al. (2006) utilized the most valid and reliable criterion measure (Cosmed® portable metabolic system), the validation study this research team conducted is deemed to have identified the most valid and reliable cut points to utilize when reducing accelerometry data.
Conclusion: Current cut-points vary widely and greatly affect the reported results of a study. Therefore, it is critical that authors reference validation studies used to support the cut-points that were employed in the data-reduction phase. Currently, validation methods have been identified using high quality criterion measures in rigorous validation studies and thus it is strongly recommended that those cut points be used in data reduction processes.
ContributorsAcuna, Kelsey Manique (Author) / Jacobson, Diana (Thesis director) / Small, Leigh (Committee member) / Barrett, The Honors College (Contributor) / Arizona State University. College of Nursing & Healthcare Innovation (Contributor) / W. P. Carey School of Business (Contributor)
Created2014-05
Description
This blog is to be used as a resource for communication
etworking and a tool for stress coping methods.
With this blog, it is my objective to aid my peers who might need help recognizing and coping with stress by the following methods:
a) Actualize the burden of Stress—Chronic stress is a burden and can be overwhelming if not managed. By disclosing my own stressors, it is my hope that peers will identify with me, so that I can then change the way they view and handle the stress.
b) Discuss the psychological and physical effects of stress on the body—It is my intent to clarify how unmanaged chronic stress can affect the physical and mental health and how acute stress is normal and healthy.
c) Share my coping methods that I have found effective in five minute or less videos with blurbs about how and why they are effective. I believe showing them to you in these mostly raw and unedited videos help maintain the current theme I am going for—keep things as real and raw as possible. Hopefully, these raw videos will help peers visualize working coping methods!
etworking and a tool for stress coping methods.
With this blog, it is my objective to aid my peers who might need help recognizing and coping with stress by the following methods:
a) Actualize the burden of Stress—Chronic stress is a burden and can be overwhelming if not managed. By disclosing my own stressors, it is my hope that peers will identify with me, so that I can then change the way they view and handle the stress.
b) Discuss the psychological and physical effects of stress on the body—It is my intent to clarify how unmanaged chronic stress can affect the physical and mental health and how acute stress is normal and healthy.
c) Share my coping methods that I have found effective in five minute or less videos with blurbs about how and why they are effective. I believe showing them to you in these mostly raw and unedited videos help maintain the current theme I am going for—keep things as real and raw as possible. Hopefully, these raw videos will help peers visualize working coping methods!
ContributorsLilley, Angela Marie (Author) / Stevens, Carol (Thesis director) / Borgiali, Daniela (Committee member) / Flucker, Ruth (Committee member) / Barrett, The Honors College (Contributor) / Arizona State University. College of Nursing & Healthcare Innovation (Contributor)
Created2013-05
Description
The purpose of this study is to learn about registered nurses' thoughts and experiences about the use of music and music therapy in the nursing field and whether or not nurses believe that there is supporting evidence practicing music therapy. Through an online survey administered to a local chapter of Sigma Theta Tau International Nursing Honors Society via SurveyMonkey, the study will 1) Assess the awareness and knowledge that registered nurses have regarding the use of music in the medical field. 2) Understand bedside nurses' willingness to use music as a complementary medicine in their own practices. 3) Ascertain where and how bedside nurses get knowledge about the use of music in the medical field. 4) Determine what constraints or barriers may influence bedside nurses' utilization of music in their practice. 5) The study will also examine the extent to which bedside nurses have had experience with observing music therapy or have utilized music therapists in their own practice.
ContributorsVintilescu, Maria Oana (Author) / Kenny, Katherine (Thesis director) / Jacobson, Diana (Committee member) / Barrett, The Honors College (Contributor) / Arizona State University. College of Nursing & Healthcare Innovation (Contributor)
Created2013-12
Description
This project focused on determining the nursing influences on first-time mothers during labor. Postpartum women at a local hospital participated by completing a reliable and valid questionnaire to rate the importance of specific nursing supportive behaviors during their labors. The data gathered from this research will provide insight to labor and delivery nurses about how to best care for their patients' emotional, physical, and informational needs
ContributorsKinney, Meghan Marie (Author) / Floegel, Theresa (Thesis director) / Jasper, Marcia (Committee member) / Barrett, The Honors College (Contributor) / Arizona State University. College of Nursing & Healthcare Innovation (Contributor)
Created2013-12
Description
AMPylation is a post-translation modification that has an important role in the survival of many bacterial pathogens by affecting the host cell's molecular signaling. In the course of studying this intercellular manipulation, there has only been modest progression in the identification of the enzymes with AMPylation capabilities (AMPylators) and their respective targets. The reason for these minimal developments is the inability to analyze a large subset of these proteins. Therefore, to increase the efficiency of the identification and characterization of the proteins, Yu et al developed a high-throughput non-radioactive discovery platform using Human Nucleic Acid Programmable Protein Arrays (NAPPA) and a validation platform using bead-based assays. The large-scale unbiased screening of potential substrates for two bacterial AMPylators containing Fic domain, VopS and IbpAFic2, had been performed and dozens of novel substrates were identified and confirmed. With the efficiency of this method, the platform was extended to the identification of novel substrates for a Legionella virulence factor, SidM, containing a different adenylyl transferase domain. The screening was performed using NAPPA arrays comprising of 10,000 human proteins, the active AMPylator SidM, and its inactive D110/112A mutant as a negative control. Many potential substrates of SidM were found, including Rab GTPases and non-GTPase proteins. Several of which have been confirmed with the bead-based AMPylation assays.
ContributorsGraves, Morgan C. (Author) / LaBaer, Joshua (Thesis director) / Qiu, Ji (Committee member) / Yu, Xiaobo (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2013-05
Description
The stigma associated with mental illness has been and continues to be a considerable issue of concern in the care of persons with mental illness. Stigma affects not only those with mental illness, but also their families, healthcare personnel, the social community, and policy formation. Common themes of stigma associated with mental illness are fear, social rejection, stereotyping, negative impact, and a lack of knowledge and awareness of mental illness. Despite a more accurate understanding of mental illness, stigma still exists. Interventions to help reverse the stigma associated with mental illness include education, awareness and an environment of inclusion. Toward this end, a PowerPoint presentation will be gifted to Arizona State University College of Nursing to be shown during the psychiatric mental health rotation outlining mental illness and stigma, and what nurses and future nurses can do to combat this stigma.
ContributorsParsons, Chelsey (Author) / Fargotstein, Barbara (Thesis director) / Hosley, Brenda (Committee member) / Sayles, Judy (Committee member) / Barrett, The Honors College (Contributor) / Arizona State University. College of Nursing & Healthcare Innovation (Contributor)
Created2014-12
Description
This creative project examines the effectiveness of several school based nutritional education and parent based programs along with multi-component interventions. Six published articles were reviewed and summarized to identify the most successful interventions to prevent childhood obesity. In addition to these studies other resources were examined to understand the developmental levels of school-aged and adolescent students. As part of this project a narrated power point covering the key aspects of the nutritional needs of the school-aged child was developed. This power point will be utilized by future nursing students whom are working with parents in the schools or in the community on nutrition strategies. The power point will provide a context for individual or group discussions with parents to offer helpful ideas on how to work effectively with their children. The topic of nutrition and obesity in school-aged children is a current topic in health care especially in environments where nutritional resources are limited. The overall outcome of this project will be to assist in decreasing the incidence of overweight and obese youth and the prevention of the development of premature chronic diseases especially in early adolescence and young adulthood.
ContributorsLopez, Leticia Marie (Author) / Hosley, Brenda (Thesis director) / Speer, Therese (Committee member) / Storto, Pamela (Committee member) / Barrett, The Honors College (Contributor) / Arizona State University. College of Nursing & Healthcare Innovation (Contributor)
Created2014-12
Description
The purpose of this project was to identify proteins associated with the migration and invasion of non-transformed MCF10A mammary epithelial cells with ectopically expressed missense mutations in p53. Because of the prevalence of TP53 missense mutations in basal-like and triple-negative breast cancer tumors, understanding the effect of TP53 mutations on the phenotypic expression of human mammary epithelial cells may offer new therapeutic targets for those currently lacking in treatment options. As such, MCF10A mammary epithelial cells ectopically overexpressing structural mutations (G245S, H179R, R175H, Y163C, Y220C, and Y234C) and DNA-binding mutations (R248Q, R248W, R273C, and R273H) in the DNA-binding domain were selected for use in this project. Overexpression of p53 in the mutant cell lines was confirmed by western blot and q-PCR analysis targeting the V5 epitope tag present in the pLenti4 vector used to transduce TP53 into the mutant cell lines. Characterization of the invasion and migration phenotypes resulting from the overexpression of p53 in the mutant cell lines was achieved using transwell invasion and migration assays with Boyden chambers. Statistical analysis showed that three cell lines—DNA-contact mutants R248W and R273C and structural mutant Y220C—were consistently more migratory and invasive and demonstrated a relationship between the migration and invasion properties of the mutant cell lines. Two families of proteins were then explored: those involved in the Epithelial-Mesenchymal Transition (EMT) and matrix metalloproteinases (MMPs). Results of q-PCR and immunofluorescence analysis of epithelial marker E-cadherin and mesenchymal proteins Slug and Vimentin did not show a clear relationship between mRNA and protein expression levels with the migration and invasiveness phenotypes observed in the transwell studies. Results of western blotting, q-PCR, and zymography of MMP-2 and MMP-9 also did not show any consistent results indicating a definite relationship between MMPs and the overall invasiveness of the cells. Finally, two drugs were tested as possible treatments inhibiting invasiveness: ebselen and SBI-183. These drugs were tested on only the most invasive of the MCF10A p53 mutant cell lines (R248W, R273C, and Y220C). Results of invasion assay following 30 μM treatment with ebselen and SBI-183 showed that ebselen does not inhibit invasiveness; SBI-183, however, did inhibit invasiveness in all three cell lines tested. As such, SBI-183 will be an important compound to study in the future as a treatment that could potentially serve to benefit triple-negative or basal-like breast cancer patients who currently lack therapeutic treatment options.
ContributorsZhang, Kathie Q (Author) / LaBaer, Joshua (Thesis director) / Anderson, Karen (Committee member) / Gonzalez, Laura (Committee member) / Barrett, The Honors College (Contributor) / School of International Letters and Cultures (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2015-05
Description
In the digital humanities, there is a constant need to turn images and PDF files into plain text to apply analyses such as topic modelling, named entity recognition, and other techniques. However, although there exist different solutions to extract text embedded in PDF files or run OCR on images, they typically require additional training (for example, scholars have to learn how to use the command line) or are difficult to automate without programming skills. The Giles Ecosystem is a distributed system based on Apache Kafka that allows users to upload documents for text and image extraction. The system components are implemented using Java and the Spring Framework and are available under an Open Source license on GitHub (https://github.com/diging/).
ContributorsLessios-Damerow, Julia (Contributor) / Peirson, Erick (Contributor) / Laubichler, Manfred (Contributor) / ASU-SFI Center for Biosocial Complex Systems (Contributor)
Created2017-09-28