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Description
Remembered as a virtuoso pianist from the Romantic period, Anton Rubinstein (1829-1894) was well-known for his outstanding piano technique and his series of historic recitals. Rubinstein was also a prolific composer, and piano music constitutes a substantial portion of his output. Scholars and pianists recently have shown interest in Rubinstein’s music, primarily his piano sonatas and études. His Morceaux, however, receive little notice and are seldom performed by pianists today. The project at hand examines four pieces: Impromptu and Serenade from Morceaux, Op. 16, and Ballade and Variations from Morceaux, Op. 104. These works are explored from both compositional and performance perspectives. After a brief introduction about Rubinstein’s life and his piano music, the four pieces from these two sets of Morceaux are described analytically. Performance suggestions follow the description of each piece, concentrating on the technical and expressive challenges for pianists.
ContributorsCai, Jun (Author) / Holbrook, Amy (Thesis advisor) / Meir, Baruch (Thesis advisor) / Creviston, Hannah (Committee member) / Arizona State University (Publisher)
Created2021
Description
Abiotic stresses, such as heat, can drive protein misfolding and aggregation, leading to inhibition of cellular function and ultimately cell death. Unexpectedly, a thermotolerant Escherichia coli was identified from a pool of antibiotic resistant RNA polymerase β subunit (rpoB) mutants. This stress tolerant phenotype was characterized through exposure to high temperature and ethanol. After 30-minute exposure of cells to 55°C or 25% ethanol, the mutant displayed 100 times greater viability than the wild-type, indicating that the rpoB mutation may have broadly affected the cellular environment to reduce protein misfolding and/or prevent protein aggregation. To further test this hypothesis, we examined thermotolerance of cells lacking heat shock chaperone DnaJ (Hsp40), which is a cochaperone of one of the most abundant and conserved chaperones, DnaK (Hsp70). The deletion of dnaJ led to severe growth defects in the wild-type, namely a slower growth rate and extreme filamentation at 42°C. The severity of the growth defects increased after additionally deleting DnaJ analog, CbpA. However, these defects were significantly ameliorated by the rpoB mutation. Finally, the rpoB mutant was found to be minimally affected by the simultaneous depletion of DnaK and DnaJ compared to the wild-type, which failed to form single colonies at 37°C and 42°C. Based on these observations, it is proposed that the rpoB mutant’s robust thermotolerant phenotype results from a cellular environment protective against protein aggregation or improper folding. The folding environment of the rpoB mutants should be further examined to elucidate the mechanism by which both antibiotic resistance and thermotolerance can be conferred.
ContributorsYeh, Melody (Author) / Misra, Rajeev (Thesis director) / Wang, Xuan (Committee member) / Kelly, Keilen (Committee member) / School of Life Sciences (Contributor) / School of International Letters and Cultures (Contributor) / School of Human Evolution & Social Change (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05