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Description
This survey was used to identify the concerns of the public. There are many population techniques that the public feels are harmful to the behavior and nature of the horses. This research defined the different techniques and history of the situation to then analyze the public's opinion of the topic. The survey was trying to determine if any of the techniques could be accepted by the public to control the population of the horses that won’t put the forest, other species, and the herds themselves in danger of possible overpopulation effects.
The data collected from this research showed conflict for the subject. Some of the data was skewed due to conflicting viewpoints on the topic. Analyzing the other aspects of the data we saw noticed that 73% of the servers felt the horses were wild. A majority agreed that the horses should stay there and not be relocated. Some individuals who took the survey were interested in adopting out the horses to help manage horses, there were some concerns with background checks for these adopters since slaughter houses and poor living conditions is a concern.
The data collected from this research showed conflict for the subject. Some of the data was skewed due to conflicting viewpoints on the topic. Analyzing the other aspects of the data we saw noticed that 73% of the servers felt the horses were wild. A majority agreed that the horses should stay there and not be relocated. Some individuals who took the survey were interested in adopting out the horses to help manage horses, there were some concerns with background checks for these adopters since slaughter houses and poor living conditions is a concern.
ContributorsHoover, Sierra Nicole (Co-author) / Padayachee, Brittany (Co-author) / Roen, Duane (Thesis director) / Murphree, Julie (Committee member) / College of Integrative Sciences and Arts (Contributor) / Barrett, The Honors College (Contributor)
Created2020-12
Description
The purpose of this project was to create a workshop for adults without dance experience to immerse them in dance as a form of therapy. The goals of this workshop are to foster physical awareness and physical creativity. There are four main areas the participants would get experience in from this workshop including self-care/awareness, expression, gesture and choreography. These four areas are the ways that the two main goals manifest themselves. For this project I reviewed research by many different professionals from the therapy, psychology, self-care, dance, and dance therapy fields. Their studies as well as my own experience aided in putting together my themes for the workshop and class activities. I include my methodology, lesson plans, a workbook, and my reflection on the process.
ContributorsWilson, Deepika (Author) / Roses-Thema, Cynthia (Thesis director) / Giorgis, Cyndi (Committee member) / College of Integrative Sciences and Arts (Contributor) / Barrett, The Honors College (Contributor)
Created2020-12
Description
Particulate matter (PM) air pollution is a known factor to exacerbate cardiopulmonary diseases. We previously demonstrated that PM mediated endothelial injury and barrier disruption via modulation of the endothelial cytoskeleton and cell-cell junctions, while the effects of PM exposure on cell-cell communication and gap junction activity are still unknown. This study is focused on the characterization of PM-mediated endothelial dysfunction via Connexin 43 (Cx43), the most abundant Gap junction protein expressed in lung endothelial cells (ECs). PM exposure induces a time-dependent elevation of Cx43 in human lung ECs, at both mRNA and protein levels. N-acetyl-cysteine (NAC), an ROS scavenger, significantly suppresses PM-induced Cx43 expression. Membrane-associated and ER/ Golgi apparatus Cx43 protein are elevated upon PM challenge. In addition, PM also activates the gap junction activity, indicated by the transportation of green fluorescence dye between two adjacent ECs. Moreover, GAP27, a selective Cx43 channel inhibitor, attenuates PM-reduced human lung EC barrier disruption, measured by trans-endothelial electrical resistance (TER) with an electric cell-substrate impedance sensing system. Moreover, knock-down the expression of Cx43 by its selective siRNA alleviates PM-induced MLC phosphorylation. These results highly suggest that Cx43 plays a key role in PM-mediated endothelial barrier disruption and signal transduction. Cx43 may deputy as a therapeutic target in PM-mediated cardiopulmonary disorders.
ContributorsKheshtchin-Kamel, Nabia (Author) / Welcome, Natalie (Thesis director) / Wang, Ting (Committee member) / College of Integrative Sciences and Arts (Contributor) / Barrett, The Honors College (Contributor)
Created2020-12
Description
“Sam’s Emotional Day: A Child’s Guide to Recognizing and Handling Emotions” is a creative project that fulfills the thesis requirement for Barrett, The Honors College at Arizona State University. It is an illustrated children’s book meant to help children ages 4-7 identify, understand, and manage the six basic emotions. The illustrations of the main character throughout the book display clear facial expressions associated with each emotion, which were designed for children to easily identify them. The text in the story portion will prompt children to understand the feelings behind each emotion in a simple matter, and the end of the book provides techniques for them to manage emotions. Research on the importance of teaching children emotions, how to teach them, and similar children’s literature are discussed. Facial expressions and feelings while experiencing an emotion was also researched, which is presented in the creative process. An analysis of the text and illustrations is provided as well.
ContributorsMartinson, Hannah Marie (Author) / Jimenez-Arista, Laura (Thesis director) / Pereira, Jennifer (Committee member) / College of Integrative Sciences and Arts (Contributor) / Barrett, The Honors College (Contributor)
Created2020-12
Description
Serial femtosecond X-ray crystallography (SFX) using an X-ray free electron laser (XFEL) is a recent advancement in structural biology for solving crystal structures of challenging membrane proteins, including G-protein coupled receptors (GPCRs), which often only produce microcrystals. An XFEL delivers highly intense X-ray pulses of femtosecond duration short enough to enable the collection of single diffraction images before significant radiation damage to crystals sets in. Here we report the deposition of the XFEL data and provide further details on crystallization, XFEL data collection and analysis, structure determination, and the validation of the structural model. The rhodopsin-arrestin crystal structure solved with SFX represents the first near-atomic resolution structure of a GPCR-arrestin complex, provides structural insights into understanding of arrestin-mediated GPCR signaling, and demonstrates the great potential of this SFX-XFEL technology for accelerating crystal structure determination of challenging proteins and protein complexes.
ContributorsZhou, X. Edward (Author) / Gao, Xiang (Author) / Barty, Anton (Author) / Kang, Yanyong (Author) / He, Yuanzheng (Author) / Liu, Wei (Author) / Ishchenko, Andrii (Author) / White, Thomas A. (Author) / Yefanov, Oleksandr (Author) / Han, Gye Won (Author) / Xu, Qingping (Author) / de Waal, Parker W. (Author) / Suino-Powell, Kelly M. (Author) / Boutet, Sebastien (Author) / Williams, Garth J. (Author) / Wang, Meitian (Author) / Li, Dianfan (Author) / Caffrey, Martin (Author) / Chapman, Henry N. (Author) / Spence, John (Author) / Fromme, Petra (Author) / Weierstall, Uwe (Author) / Stevens, Raymond C. (Author) / Cherezov, Vadim (Author) / Melcher, Karsten (Author) / Xu, H. Eric (Author) / College of Liberal Arts and Sciences (Contributor) / School of Molecular Sciences (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / Department of Physics (Contributor)
Created2016-04-12
Description
Diacylglycerol kinase catalyses the ATP-dependent conversion of diacylglycerol to phosphatidic acid in the plasma membrane of Escherichia coli. The small size of this integral membrane trimer, which has 121 residues per subunit, means that available protein must be used economically to craft three catalytic and substrate-binding sites centred about the membrane/cytosol interface. How nature has accomplished this extraordinary feat is revealed here in a crystal structure of the kinase captured as a ternary complex with bound lipid substrate and an ATP analogue. Residues, identified as essential for activity by mutagenesis, decorate the active site and are rationalized by the ternary structure. The γ-phosphate of the ATP analogue is positioned for direct transfer to the primary hydroxyl of the lipid whose acyl chain is in the membrane. A catalytic mechanism for this unique enzyme is proposed. The active site architecture shows clear evidence of having arisen by convergent evolution.
ContributorsLi, Dianfan (Author) / Stansfeld, Phillip J. (Author) / Sansom, Mark S. P. (Author) / Keogh, Aaron (Author) / Vogeley, Lutz (Author) / Howe, Nicole (Author) / Lyons, Joseph A. (Author) / Aragao, David (Author) / Fromme, Petra (Author) / Fromme, Raimund (Author) / Basu, Shibom (Author) / Grotjohann, Ingo (Author) / Kupitz, Christopher (Author) / Rendek, Kimberley (Author) / Weierstall, Uwe (Author) / Zatsepin, Nadia (Author) / Cherezov, Vadim (Author) / Liu, Wei (Author) / Bandaru, Sateesh (Author) / English, Niall J. (Author) / Gati, Cornelius (Author) / Barty, Anton (Author) / Yefanov, Oleksandr (Author) / Chapman, Henry N. (Author) / Diederichs, Kay (Author) / Messerschmidt, Marc (Author) / Boutet, Sebastien (Author) / Williams, Garth J. (Author) / Seibert, M. Marvin (Author) / Caffrey, Martin (Author) / College of Liberal Arts and Sciences (Contributor) / School of Molecular Sciences (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / Department of Physics (Contributor)
Created2015-12-17
Description
Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophore-binding domains of the Deinococcus radiodurans phytochrome at 2.1 Å resolution. The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser. We find overall good agreement compared to a crystal structure at 1.35 Å resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX.
ContributorsEdlund, Petra (Author) / Takala, Heikki (Author) / Claesson, Elin (Author) / Henry, Leocadie (Author) / Dods, Robert (Author) / Lehtivuori, Heli (Author) / Panman, Matthijs (Author) / Pande, Kanupriya (Author) / White, Thomas (Author) / Nakane, Takanori (Author) / Berntsson, Oskar (Author) / Gustavsson, Emil (Author) / Bath, Petra (Author) / Modi, Vaibhav (Author) / Roy Chowdhury, Shatabdi (Author) / Zook, James (Author) / Berntsen, Peter (Author) / Pandey, Suraj (Author) / Poudyal, Ishwor (Author) / Tenboer, Jason (Author) / Kupitz, Christopher (Author) / Barty, Anton (Author) / Fromme, Petra (Author) / Koralek, Jake D. (Author) / Tanaka, Tomoyuki (Author) / Spence, John (Author) / Liang, Mengning (Author) / Hunter, Mark S. (Author) / Boutet, Sebastien (Author) / Nango, Eriko (Author) / Moffat, Keith (Author) / Groenhof, Gerrit (Author) / Ihalainen, Janne (Author) / Stojkovic, Emina A. (Author) / Schmidt, Marius (Author) / Westenhoff, Sebastian (Author) / College of Liberal Arts and Sciences (Contributor) / School of Molecular Sciences (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / Department of Physics (Contributor)
Created2016-10-19
Description
Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.
ContributorsNogly, Przemyslaw (Author) / Panneels, Valerie (Author) / Nelson, Garrett (Author) / Gati, Cornelius (Author) / Kimura, Tetsunari (Author) / Milne, Christopher (Author) / Milathianaki, Despina (Author) / Kubo, Minoru (Author) / Wu, Wenting (Author) / Conrad, Chelsie (Author) / Coe, Jesse (Author) / Bean, Richard (Author) / Zhao, Yun (Author) / Bath, Petra (Author) / Dods, Robert (Author) / Harimoorthy, Rajiv (Author) / Beyerlein, Kenneth R. (Author) / Rheinberger, Jan (Author) / James, Daniel (Author) / Deponte, Daniel (Author) / Li, Chufeng (Author) / Sala, Leonardo (Author) / Williams, Garth J. (Author) / Hunter, Mark S. (Author) / Koglin, Jason E. (Author) / Berntsen, Peter (Author) / Nango, Eriko (Author) / Iwata, So (Author) / Chapman, Henry N. (Author) / Fromme, Petra (Author) / Frank, Matthias (Author) / Abela, Rafael (Author) / Boutet, Sebastien (Author) / Barty, Anton (Author) / White, Thomas A. (Author) / Weierstall, Uwe (Author) / Spence, John (Author) / Neutze, Richard (Author) / Schertler, Gebhard (Author) / Standfuss, Jorg (Author) / College of Liberal Arts and Sciences (Contributor) / Department of Physics (Contributor) / Department of Chemistry and Biochemistry (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / School of Molecular Sciences (Contributor)
Created2016-08-22
Description
The United Nations outlined seventeen goals in their 2015 resolution aimed at securing a more sustainable future for the entire world. These goals cover all parts of life and are meant to standardize quality of life at a high level for all countries. Specific to the Maasai people of Kenya and Tanzania, there are five main goals that would tackle some of the biggest issues facing the Maasai and greatly improve their lives. These goals include the promotion of good health and well-being, ensuring a quality education for school-age children, supporting efforts for gender equality in all communities, and securing clean and safe water and sanitation services for all people around the world. There are many Non-Governmental Organizations (NGOs) in the Maasai community that are striving to support them and realize these Sustainable Development Goals. Organizations like the Maasai Girls Education Fund and Aid Tanzania have established educational programs to support children, mainly girls, in their efforts to get an education. Others like End Water Poverty and the Maasai Association have programs to ensure safe, accessible drinking water is made available to Maasai communities across Kenya and Tanzania. Lastly, NGOs like My Chosen Vessels have programs that support the economic sustainability and livestock management of the Maasai while also ensuring that Maasai families receive clean water and access to an education. The work and practices of NGOs like these can serve as examples of how to efficiently and effectively address and tackle issues in developing countries, specifically regarding Maasai women and girls, to move closer to achieving these UN Sustainable Development Goals and lead a more sustainable future. Many studies have been done researching the political and economic struggles for Maasai communities and how it impacts them. Vandana Shiva coined the term, “Water Democracy” in her book Water Wars to express the multiple reasons why water is a necessity and right. Many Maasai groups do not have easy access to basic essentials for their everyday lives and NGOs have been noticing this more and more. Dorothy Hodgson writes about the history of Maasai NGOs, and mentions the multiple conflicts that they have had with each other, causing many Maasai groups to have different lifestyles. The connections between the history of the Maasai tribe, their current economy and their current relationship with the government and NGOs all impact women in their communities in different ways.
ContributorsWacloff, Jianna K (Author) / Martin, Thomas (Thesis director) / Oberle, Eric (Committee member) / College of Integrative Sciences and Arts (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
This policy proposal paper is designed to address concerns about the protection of data
concerning citizens of the United States. The first step is to explore the need for federal
legislation because of the problems of cyberattacks, data loss and leakage, and big data. The
proposal then analyses how other countries had already addressed these concerns for their
citizens through legislation by looking at their regulation and the results of implementation. The
paper adjacently discusses the importance of American values of privacy as a fundamental right,
the free market, and protection from the private sector within a cybersecurity paradigm. From
this combined research, the paper yields a proposal of how the U.S. government should address
the situation through federal policy. The policy outlines cybersecurity measures to protect
information from cyberattacks and data loss and leakage, rights of American citizens that
organizations need to uphold, and the creation of a commission that provides resources and
education to domestic and foreign organizations.
concerning citizens of the United States. The first step is to explore the need for federal
legislation because of the problems of cyberattacks, data loss and leakage, and big data. The
proposal then analyses how other countries had already addressed these concerns for their
citizens through legislation by looking at their regulation and the results of implementation. The
paper adjacently discusses the importance of American values of privacy as a fundamental right,
the free market, and protection from the private sector within a cybersecurity paradigm. From
this combined research, the paper yields a proposal of how the U.S. government should address
the situation through federal policy. The policy outlines cybersecurity measures to protect
information from cyberattacks and data loss and leakage, rights of American citizens that
organizations need to uphold, and the creation of a commission that provides resources and
education to domestic and foreign organizations.
ContributorsBrockman, Emily M. (Author) / Hollinger, Keith (Thesis director) / Walsh, Tatiana (Committee member) / Martin, Thomas (Committee member) / College of Integrative Sciences and Arts (Contributor) / Information Technology (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05