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Description
The City of Phoenix Street Transportation Department partnered with the Rob and Melani Walton Sustainability Solutions Service at Arizona State University (ASU) and researchers from various ASU schools to evaluate the effectiveness, performance, and community perception of the new pavement coating. The data collection and analysis occurred across multiple neighborhoods and at varying times across days and/or months over the course of one year (July 15, 2020–July 14, 2021), allowing the team to study the impacts of the surface treatment under various weather conditions.
ContributorsMiddel, Ariane (Author) / Vanos, Jennifer K. (Author) / Hondula, David M. (Author) / Kaloush, Kamil (Author) / Sailor, David (Author) / Medina, Jose R. (Jose Roberto) (Author) / Schneider, Florian (Author) / Ortiz, Johny Cordova (Author) / Campbell, Bill (Author) / Epel, Erin (Contributor) / Rice, Brendan (Contributor) / Garcia, Ruth (Contributor) / Phoenix (Ariz.). Street Transportation Department (Contributor) / City of Phoenix (Funder) / Industrial Development Authority of the County of Maricopa (Funder) / Julie Ann Wrigley Global Institute of Sustainability (Funder) / Arizona State University. Healthy Urban Environments Program (Contributor)
Created2021-09
Description
Mycobacterial infections, as represented by leprosy and tuberculosis, have persisted as human pathogens for millennia. Their environmental counterparts, nontuberculous mycobacteria (NTM), are commodious infectious agents endowed with extensive innate and acquired antimicrobial resistance. The current drug development process selects for antibiotics with high specificity for definitive targets within bacterial metabolic and replication pathways. Because these compounds demonstrate limited efficacy against mycobacteria, novel antimycobacterial agents with unconventional mechanisms of action were identified. Two highly resistant NTMs, Mycobacterium abscessus (Mabs) a rapid-growing respiratory, skin, and soft tissue pathogen, and Mycobacterium ulcerans (MU), the causative agent of Buruli ulcer, were selected as targets. Compounds that indicated antimicrobial activity against other highly resistant pathogens were selected for initial screening. Antimicrobial peptides (AMPs) have demonstrated activity against a variety of bacterial pathogens, including mycobacterial species. Designed antimicrobial peptides (dAMPs), rationally-designed and synthetic contingents, combine iterative features of natural AMPs to achieve superior antimicrobial activity in resistant pathogens. Initial screening identified two dAMPs, RP554 and RP557, with bactericidal activity against Mabs. Clay-associated ions have previously demonstrated bactericidal activity against MU. Synthetic and customizable aluminosilicates have also demonstrated adsorption of bacterial cells and toxins. On this basis, two aluminosilicate materials, geopolymers (GP) and ion-exchange nanozeolites (IE-nZeos), were screened for antimicrobial activity against MU and its fast-growing relative, Mycobacterium marinum (Mmar). GPs demonstrated adsorption of MU cells and mycolactone, a secreted, lipophilic toxin, whereas Cu-nZeos and Ag-nZeos demonstrated antibacterial activity against MU and Mmar. Cumulatively, these results indicate that an integrative drug selection process may yield a new generation of antimycobacterial agents.
ContributorsDermody, Roslyn June (Author) / Haydel, Shelley E (Thesis advisor) / Bean, Heather (Committee member) / Nickerson, Cheryl (Committee member) / Stephanopoulos, Nicholas (Committee member) / Arizona State University (Publisher)
Created2022
Description
Mycobacterium tuberculosis (Mtb), the etiological agent of the tuberculosis disease, is estimated to infect one-fourth of the human population and is responsible for 1.5 million deaths annually. The increased emergence of bacterial resistance to clinical interventions highlights the lack in development of novel antimicrobial therapeutics. Prototypical bacterial two-component systems (TCS) allow for sensing of extracellular stimuli and relay thereof to create a transcriptional response. The prrAB TCS is essential for viability in Mtb, presenting itself as an attractive novel drug target. In Mtb, PrrAB is involved in the adaptation to the intra-macrophage environment and recent work implicates PrrAB in the dosR-dependent hypoxia adaptation. This work defines a direct molecular and regulatory connection between Mtb PrrAB and the dosR-dependent hypoxia response. Using electrophoretic mobility shift assays combined with surface plasmon resonance, the Mtb dosR gene is established as a specific target of PrrA, corroborated by fluorescence reporter assays demonstrating a regulatory relationship. Considering the scarce understanding of prrAB essentiality in nontuberculous mycobacteria and the presence of multiple prrAB orthologs in Mycobacterium smegmatis and Mycobacterium abscessus, CRISPR interference was utilized to evaluate the essentiality of PrrAB beyond Mtb. prrAB was found to be inessential for viability in M. smegmatis yet required for in vitro growth. Conversely, M. abscessus prrAB repression led to enhanced in vitro growth. Diarylthiazole-48 (DAT-48) displayed decreased selectivity against M. abscessus but demonstrated enhanced intrinsic activity upon prrAB repression in M. abscessus. Lastly, to aid in the rapid determination of mycobacterial drug susceptibility and the detection of mycobacterial heteroresistance, the large volume scattering imaging (LVSim) platform was adapted for mycobacteria. Using LVSim, Mtb drug susceptibility was detected phenotypically within 6 hours, and clinically relevant mycobacterial heteroresistance was detected phenotypically within 10 generations. The data generated in these studies provide insight into the essential role of PrrAB in Mtb and its involvement in the dosR-dependent hypoxia adaptation, advance the understanding of mycobacterial PrrAB essentiality and PrrAB-associated mycobacterial growth dependency. These studies further establish molecular and mechanistic connection between PrrAB and DAT-48 in Mtb and M. abscessus and develop a rapid phenotypic drug susceptibility testing platform for mycobacteria.
ContributorsHaller, Yannik Alex (Author) / Haydel, Shelley E (Thesis advisor) / Bean, Heather (Committee member) / Nickerson, Cheryl (Committee member) / Plaisier, Christopher (Committee member) / Acharya, Abhinav (Committee member) / Arizona State University (Publisher)
Created2023
Description
Transorbital surgery has gained recent notoriety due to its incorporation into endoscopic skull base surgery. The body of published literature on the field is cadaveric and observation. The pre-clinical studies are focused on the use of the endoscope only. Furthermore the methodology utilised in the published literature is inconsistent and does not embody the optimal principles of scientific experimentation. This body of work evaluates a minimally invasive novel surgical corridor - the transorbital approach - its validity in neurosurgical practice, as well as both qualitatively and quantitatively assessing available technological advances in a robust experimental fashion. While the endoscope is an established means of visualisation used in clinical transorbital surgery, the microscope has never been assessed with respect to the transorbital approach. This question is investigated here and the anatomical and surgical benefits and limitations of microscopic visualisation demonstrated. The comparative studies provide increased knowledge on specifics pertinent to neurosurgeons and other skull base specialists when planning pre-operatively, such as pathology location, involved anatomical structures, instrument maneuvrability and the advantages and disadvantages of the distinct visualisation technologies. This is all with the intention of selecting the most suitable surgical approach and technology, specific to the patient, pathology and anatomy, so as to perform the best surgical procedure. The research findings illustrated in this body of work are diverse, reproducible and applicable. The transorbital surgical corridor has substantive potential for access to the anterior cranial fossa and specific surgical target structures. The neuroquantitative metrics investigated confirm the utility and benefits specific to the respective visualisation technologies i.e. the endoscope and microscope. The most appropriate setting wherein the approach should be used is also discussed. The transorbital corridor has impressive potential, can utilise all available technological advances, promotes multi-disciplinary co-operation and learning amongst clinicians and ultimately, is a means of improving operative patient care.
ContributorsHoulihan, Lena Mary (Author) / Preul, Mark C. (Thesis advisor) / Vernon, Brent (Thesis advisor) / O' Sullivan, Michael G.J. (Committee member) / Lawton, Michael T. (Committee member) / Santarelli, Griffin (Committee member) / Smith, Brian (Committee member) / Arizona State University (Publisher)
Created2021
Description
Given increasing frequency and intensity of extreme weather events and disparities in socio-economic conditions, managing flood risks has become ever more challenging. Building a community flood resilience becomes an essential strategy to reduce flood risks and achieve sustainable development. However, enhancing community flood resilience presents numerous obstacles and potential downsides. To address such a challenge, this dissertation investigates the interactions among natural, built environment, and actor systems using a case study of flooding in Jakarta, Indonesia, focusing on the Ciliwung river watershed. The study first develops a conceptual framework for community flood resilience by integrating concepts from disaster, flood, and community resilience research to represent how water, structural elements, and actors are connected and can contribute to hazards and vulnerabilities. Building on the framework, the study utilizes a mixed-methods approach, combining stakeholder interviews with network and heatmap analysis to identify the roles and interconnections of relevant actors in Jakarta’s flooding. Next, the study employs a hybrid of agent-based and system dynamics modeling to explore the complex interactions among the watershed’s physical and actor systems. The model evaluates how the level of collaboration among government agencies affects flood management interventions and community resilience under various scenarios concerning weather patterns and land-use change.
The research provides an exploratory tool and a strategic guide for enhancing community flood resilience. The stakeholder analysis reveals the complexity of stakeholder relationships and challenges such as imbalance in resource allocation, authority overlap, and low engagement. These insights highlight the need for trust-building, community involvement, and strategic alignment in flood management efforts. Systems modeling to derive policy recommendations bridges the gap between theoretical frameworks and governance challenges, offering empirical evidence of the critical role of governmental collaboration in reducing exposure to floods. The systems modeling results suggest that under conditions of uncertainties, enhancing community flood resilience requires a coordinated approach among stakeholders.
ContributorsSulistyo, Sinta Rahmawidya (Author) / Agusdinata, Datu Buyung (Thesis advisor) / Aggarwal, Rimjhim (Committee member) / Brundiers, Katja (Committee member) / Arizona State University (Publisher)
Created2024
Description
With less than seven years left to reach the ambitious targets of the United Nations' 2030 Sustainable Development Goals (SDGs), it is imperative to understand how the SDGs are operationalized in practice to support effective governance. One integrative approach, the water, energy, and food (WEF) nexus, has been proposed to facilitate SDGs planning and implementation by incorporating synergies, co-benefits, and trade-offs. In this dissertation, I conduct three interrelated WEF nexus studies using a sustainability lens to develop new approaches and identify actionable measures to support the SDGs. The first paper is a systematic literature review (2015 – 2022) to investigate the extent to which WEF nexus research has generated actionable knowledge to achieve the SDGs. The findings show that the WEF nexus literature explicitly considering the SDGs mainly focuses on governance and environmental protection, with fewer studies focusing on target populations and affordability. In the second paper, I reframed the water quality concerns using a nexus and systems thinking approach in a FEW nexus hotspot, the Rio Negro Basin (RNB) in Uruguay. While Uruguay is committed to the 2030 Agenda for Sustainable Development, sustainability challenges endure in managing synergies and trade-offs, resulting in strategy setbacks for the sustainable development of food, land, water, and oceans. Reframing the water quality problem facilitated the identification of potential alternative intervention points to support local problem-solving capacity. In the third paper, I conducted semi-structured interviews and examined the meeting transcripts of the RNB Commission to understand local perspectives about how the activities and initiatives taking place in the basin enhance or diminish the overall sustainability. Sustainability criteria for river basin planning and management were operationalized through qualitative appraisal questions. The case of the RNB illustrates the challenges of coordinating the national development agenda to local livelihood. This dissertation advances the WEF nexus and sustainability science literature by shedding light on the implications of the research trend to support the SDGs, as well as reframing and appraising a persistent water quality problem to support sustainable development.
ContributorsOjeda Matos, Glorynel (Author) / White, Dave D (Thesis advisor) / Brundiers, Katja (Committee member) / Garcia, Margaret (Committee member) / Arizona State University (Publisher)
Created2023
Description
A description of numerical and analytical work pertaining to models that describe the growth and progression of glioblastoma multiforme (GBM), an aggressive form of primary brain cancer. Two reaction-diffusion models are used: the Fisher-Kolmogorov-Petrovsky-Piskunov equation and a 2-population model that divides the tumor into actively proliferating and quiescent (or necrotic) cells. The numerical portion of this work (chapter 2) focuses on simulating GBM expansion in patients undergoing treatment for recurrence of tumor following initial surgery. The models are simulated on 3-dimensional brain geometries derived from magnetic resonance imaging (MRI) scans provided by the Barrow Neurological Institute. The study consists of 17 clinical time intervals across 10 patients that have been followed in detail, each of whom shows significant progression of tumor over a period of 1 to 3 months on sequential follow up scans. A Taguchi sampling design is implemented to estimate the variability of the predicted tumors to using 144 different choices of model parameters. In 9 cases, model parameters can be identified such that the simulated tumor contains at least 40 percent of the volume of the observed tumor. In the analytical portion of the paper (chapters 3 and 4), a positively invariant region for our 2-population model is identified. Then, a rigorous derivation of the critical patch size associated with the model is performed. The critical patch (KISS) size is the minimum habitat size needed for a population to survive in a region. Habitats larger than the critical patch size allow a population to persist, while smaller habitats lead to extinction. The critical patch size of the 2-population model is consistent with that of the Fisher-Kolmogorov-Petrovsky-Piskunov equation, one of the first reaction-diffusion models proposed for GBM. The critical patch size may indicate that GBM tumors have a minimum size depending on the location in the brain. A theoretical relationship between the size of a GBM tumor at steady-state and its maximum cell density is also derived, which has potential applications for patient-specific parameter estimation based on magnetic resonance imaging data.
ContributorsHarris, Duane C. (Author) / Kuang, Yang (Thesis advisor) / Kostelich, Eric J. (Thesis advisor) / Preul, Mark C. (Committee member) / Crook, Sharon (Committee member) / Gardner, Carl (Committee member) / Arizona State University (Publisher)
Created2023
Description
The learning journey toward sustainability requires the generation of action-oriented knowledge to understand the progress and potential adaptation of strategies to facilitate collective transformation. One integral area where advancing these strategies is essential is higher education. While efforts in the field have identified relevant learning objectives, pedagogies, and broader contributions for sustainability education to make, the sophistication of approaches to evaluating and enhancing collective learning has remained underdeveloped. This dissertation aimed to address this gap by exploring assessment in sustainability education, with the goal of supporting innovations in transdisciplinary practice by developing distinctive approaches for the field through deliberative processes that articulate design frameworks.The first study, Sustainability-Oriented Assessment, applied a formative intervention within an undergraduate course focused on professional skill development to demonstrate how a student-led rubric co-design process that drew from participative, normative, and integrative approaches contributed to student agency, expansive learning, and self-formation. The study demonstrated how students leveraged challenges and changing perspectives towards development as students and sustainability professionals. The second study, Boundary Crossings and Innovations, applied an exploratory action research approach within a community of practice of program leaders and instructors to identify barriers and possibilities for assessment practices, suggesting conceptual, practical, and logistical innovations. The strategies to enable these innovations focused on defining the field and articulating practice characteristics related to interdisciplinary approaches and transdisciplinary strategies, while meeting demands and facilitating innovations across course, program, and institutional levels. The third study, Assessment-Oriented Sustainability, applied a scoping literature review to develop a framework that integrates problem, solution, and learning orientations to sustainability by infusing educational purposes, processes, and principles to strengthen the efficacy of assessment approaches for transdisciplinary strategies.
Insights from the three studies suggest that the practice of assessment plays multifaceted roles as it facilitates the design, implementation, and evaluation of sustainability endeavors. These roles include evaluative and formative functions as it appraises and advances learning, normative and deliberative functions in encouraging discussion and critical reflection, and adaptive and generative functions by indicating, motivating, and enabling dynamic learning and action for sustainability.
ContributorsKing, Jordan (Author) / Fischer, Daniel (Thesis advisor) / Larson, Kelli (Thesis advisor) / Brundiers, Katja (Committee member) / Arizona State University (Publisher)
Created2024
Description
Despite the safe and effective use of attenuated vaccines for over fifty years, measles virus (MV) remains an insidious threat to global health. Problematically, infants less than one year of age, who are the most prone to severe infection and death by measles, cannot be immunized using current MV vaccines. For this dissertation, I generated and performed preclinical evaluation of two novel MV vaccine candidates. Based on data from clinical trials that showed increasing the dosage of current MV vaccines improved antibody responses in six-month-old recipients, I hypothesized that increasing the relevant antigenic stimulus of a standard titer dose would allow safe and effective immunization at a younger age. I generated two modified MVs with increased expression of the hemagglutinin (H) protein, the most important viral antigen for inducing protective neutralizing immunity, in the background of a current vaccine-equivalent. One virus, MVvac2-H2, expressed higher levels of full-length H, resulting in a three-fold increase in H incorporation into virions, while the second, MVvac2-Hsol, expressed and secreted truncated, soluble H protein to its extracellular environment. The alteration to the virion envelope of MVvac2-H2 conferred upon that virus a measurable resistance to in vitro neutralization. In initial screening in adult mouse models of vaccination, both modified MVs proved more immunogenic than their parental strain in outbred mice, while MVvac2-H2 additionally proved more immunogenic in the gold standard MV-susceptible mouse model. Remarkably, MVvac2-H2 better induced protective immunity in the presence of low levels of artificially introduced passive immunity that mimic the passive maternal immunity that currently limits vaccination of young infants, and that strongly inhibited responses to the current vaccine-equivalent. Finally, I developed a more physiological infant-like mouse model for MV vaccine testing, in which MV-susceptible dams vaccinated with the current vaccine-equivalent transfer passive immunity to their pups. This model will allow additional preclinical evaluation of the performance of MVvac2-H2 in pups of immune dams. Altogether, in this dissertation I identify a promising candidate, MVvac2-H2, for a next generation measles vaccine.
ContributorsJulik, Emily (Author) / Reyes del Valle, Jorge (Thesis advisor) / Chang, Yung (Committee member) / Blattman, Joseph (Committee member) / Hogue, Brenda (Committee member) / Nickerson, Cheryl (Committee member) / Arizona State University (Publisher)
Created2016
Description
Scholars have highlighted the role of disturbance and crisis, including disasters, in enabling systemic change towards sustainability. However, there are relatively few empirical studies on how individuals and organizations are able to utilize disasters as opportunities for change towards sustainability. This dissertation addresses three questions applied to two case studies: First, what changes were pursued in the aftermath of disasters, and to what extent did these changes contribute to sustainability? Second, how were people (and their organizations) able to pursue change towards sustainability? Third, what can be learned about seeing and seizing opportunities for change towards sustainability in disaster contexts and about sustaining those introduced changes over time?
The research entailed the creation of a theoretical framework, synthesizing literature from disaster studies and sustainability transition studies, to enable cross-case comparison and the appraisal of sustainability outcomes (Chapter 1). The framework was applied to two empirical case studies of post-disaster recovery: the 2004 Indian Ocean Tsunami in Aceh, Indonesia (Chapter 2), and the 2010-2012 series of earthquakes in the greater Christchurch area, New Zealand (Chapter 3).
The research revealed no systemic change towards sustainability in either case, although change towards sustainability was pursued in various areas, such as housing, educating, caring, and engaging in governance. Opportunities for sustainability emerged at different points following the disaster; change processes are ongoing. The sustainability changes were supported by “Sustainability Change Agents” (SCAs): people who were able to see and seize opportunities for change towards sustainability in the midst of disaster. SCAs were characterized as individuals with various attributes, starting with an ability to perceive opportunities, catalyze others to support this risk-taking endeavor, and stay in the endurance race. The study concludes with some recommendations for interventions to inform pre-disaster sustainability planning. These avenues include a toolbox and a curricular approach that would educate and enable students as future professionals to see and seize opportunities for change towards sustainability in disaster contexts (Chapter 4).
The research entailed the creation of a theoretical framework, synthesizing literature from disaster studies and sustainability transition studies, to enable cross-case comparison and the appraisal of sustainability outcomes (Chapter 1). The framework was applied to two empirical case studies of post-disaster recovery: the 2004 Indian Ocean Tsunami in Aceh, Indonesia (Chapter 2), and the 2010-2012 series of earthquakes in the greater Christchurch area, New Zealand (Chapter 3).
The research revealed no systemic change towards sustainability in either case, although change towards sustainability was pursued in various areas, such as housing, educating, caring, and engaging in governance. Opportunities for sustainability emerged at different points following the disaster; change processes are ongoing. The sustainability changes were supported by “Sustainability Change Agents” (SCAs): people who were able to see and seize opportunities for change towards sustainability in the midst of disaster. SCAs were characterized as individuals with various attributes, starting with an ability to perceive opportunities, catalyze others to support this risk-taking endeavor, and stay in the endurance race. The study concludes with some recommendations for interventions to inform pre-disaster sustainability planning. These avenues include a toolbox and a curricular approach that would educate and enable students as future professionals to see and seize opportunities for change towards sustainability in disaster contexts (Chapter 4).
ContributorsBrundiers, Katja (Author) / Eakin, Hallie C (Thesis advisor) / Sarewitz, Daniel (Committee member) / Awotona, Adenrele (Committee member) / Arizona State University (Publisher)
Created2016