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- Genre: Masters Thesis
Description
A synbody is a newly developed protein binding peptide which can be rapidly produced by chemical methods. The advantages of the synbody producing process make it a potential human proteome binding reagent. Most of the synbodies are designed to bind to specific proteins. The peptides incorporated in a synbody are discovered with peptide microarray technology. Nevertheless, the targets for unknown synbodies can also be discovered by searching through a protein mixture. The first part of this thesis mainly focuses on the process of target searching, which was performed with immunoprecipitation assays and mass spectrometry analysis. Proteins are pulled down from the cell lysate by certain synbodies, and then these proteins are identified using mass spectrometry. After excluding non-specific bindings, the interaction between a synbody and its real target(s) can be verified with affinity measurements. As a specific example, the binding between 1-4-KCap synbody and actin was discovered. This result proved the feasibility of the mass spectrometry based method and also suggested that a high throughput synbody discovery platform for the human proteome could be developed. Besides the application of synbody development, the peptide microarray technology can also be used for immunosignatures. The composition of all types of antibodies existing in one's blood is related to an individual's health condition. A method, called immunosignaturing, has been developed for early disease diagnosis based on this principle. CIM10K microarray slides work as a platform for blood antibody detection in immunosignaturing. During the analysis of an immunosignature, the data from these slides needs to be validated by using landing light peptides. The second part of this thesis focuses on the validation of the data. A biotinylated peptide was used as a landing light on the new CIM10K slides. The data was collected in several rounds of tests and indicated that the variation among landing lights was significantly reduced by using the newly prepared biotinylated peptide compared with old peptide mixture. Several suggestions for further landing light improvement are proposed based on the results.
ContributorsSun, Minyao (Author) / Johnston, Stephen Albert (Thesis advisor) / Diehnelt, Chris Wayne (Committee member) / Stafford, Phillip (Committee member) / Arizona State University (Publisher)
Created2011
Description
African Swine Fever (ASF), endemic in many African countries, is now spreading to other continents. Though ASF is capable of incurring serious economic losses in affected countries, no vaccine exists to provide immunity to animals. Disease control relies largely on rapid diagnosis and the implementation of movement restrictions and strict eradication programs. Developing a scalable, accurate and low cost diagnostic for ASF will be of great help for the current situation. CIM's 10K random peptide microarray is a new high-throughput platform that allows systematic investigations of immune responses associated with disease and shows promise as a diagnostic tool. In this study, this new technology was applied to characterize the immune responses of ASF virus (ASFV) infections and immunizations. Six sets of sera from ASFV antigen immunized pigs, 6 sera from infected pigs and 20 sera samples from unexposed pigs were tested and analyzed statistically. Results show that both ASFV antigen immunized pigs and ASFV viral infected pigs can be distinguished from unexposed pigs. Since it appears that immune responses to other viral infections are also distinguishable on this platform, it holds the potential of being useful in developing a new ASF diagnostic. The ability of this platform to identify specific ASFV antibody epitopes was also explored. A subtle motif was found to be shared among a set of peptides displaying the highest reactivity for an antigen specific antibody. However, this motif does not seem to match with any antibody epitopes predicted by a linear antibody epitope prediction.
ContributorsXiao, Liang (Author) / Sykes, Kathryn (Thesis advisor) / Zhao, Zhan-Gong (Committee member) / Stafford, Phillip (Committee member) / Arizona State University (Publisher)
Created2011
Description
Anecdotally, native Mandarin speakers have difficulty distinguishing between the “s” (as in sink) and the “th” (as in think) sounds as well as between the “a” (as in dad) and “ea” (as in dead) sounds. Here, 29 native English speakers, 52 native Mandarin speakers who live in China, and 34 native Mandarin speakers who have been living in an English language dominant environment were recruited to serve as participants. To assess the phoneme contrasts that may occur in native Mandarin speakers in China, and possible improvement in native Mandarin speakers living in an English environment, relative to Native English speakers living in America, a phoneme discrimination test was administered, three paired phonemes were used in the current study: /b/ paired with /p/ as a control pair, /æ/ paired with /ɛ/, and /θ/ paired with /s/. The results showed that native English speakers have significantly higher accuracy rates for the three paired phoneme discrimination tasks than the native Mandarin speakers who live in China. But there was no significant difference between the native English speakers and native Mandarin speakers who have lived in an English environment on the phonemes or words discriminations tasks.
ContributorsChen, Hao (Author) / Nanez, Jose (Thesis advisor) / Horne, Zachary (Thesis advisor) / Holloway, Steven (Committee member) / Arizona State University (Publisher)
Created2021
Description
Current research has identified a specific type of visual experience that leads to faster cortical processing. Specifically, performance on perceptual learning of a directional-motion leads to faster cortical processing. This is important on two levels; first, cortical processing is positively correlated with cognitive functions and inversely related to age, frontal lobe lesions, and some cognitive disorders. Second, temporal processing has been shown to be relatively stable over time. In order to expand on this line of research, we examined the effects of a different, but relevant visual experience (i.e., implied motion) on cortical processing. Previous fMRI studies have indicated that static images that imply motion activate area V5 or middle temporal/medial superior temporal complex (MT/MST+) of the visual cortex, the same brain region that is activated in response to real motion. Therefore, we hypothesized that visual experience of implied motion may parallel the positive relationship between real directional-motion and cortical processing. Seven subjects participated in a visual task of implied motion for 4 days, and a pre- and post-test of cortical processing. The results indicated that performance on implied motion is systematically different from performance on a dot motion task. Despite individual differences in performance, overall cortical processing increased from day 1 to day 4.
ContributorsVasefi, Aresh (Author) / Nanez, Jose (Thesis advisor) / Duran, Nicholas (Committee member) / Keil, Thomas J. (Committee member) / Arizona State University (Publisher)
Created2014
Description
Affiliative touch, such as physical affection between relationship partners, activates neural systems associated with reward, relaxation, and attachment. Co-sleeping is a common practice among romantic partners, and the social context of sleep is linked to well-being. The effect of touch during sleep, however, remains largely untested. As a first study, 210 married couples were asked how much they generally touched during sleep and how important it was for them to touch during sleep. I hypothesized that perceptions of more spousal touch during sleep, as well as greater importance placed on that touch, would be associated with better quality of sleep. Given the strong links between touch and attachment, and previous findings of poor sleep associated with attachment anxiety, these effects were expected to be greatest among spouses higher in attachment anxiety (who might benefit most from a sense of security arising from touch). Separate regression analyses were run for husbands and wives, controlling for affective symptoms of depression (which were significant predictors of poor sleep for both spouses). For both spouses, higher reports of amount and importance of touch during sleep predicted better quality of sleep. For wives, the predicted interaction was significant, but in the opposite direction: Reported amount and importance of spousal touch during sleep was positively related to sleep quality only among those with lower attachment anxiety, whereas it was unrelated among those with higher attachment anxiety. Higher attachment anxiety also was related to worse sleep among wives, but not husbands. It may be the case that wives who are lowest in attachment anxiety may feel more comfortable when being touched by their partners. As a result, they may touch more often, place more importance on touch, and be more likely to experience rewards of touch such as better sleep quality. The findings lend support to the idea that social touch can serve a regulatory function, even during sleep.
ContributorsShahid, Shiza (Author) / Burleson, Mary H (Thesis advisor) / Roberts, Nicole A. (Committee member) / Nanez, Jose (Committee member) / Arizona State University (Publisher)
Created2017
Description
Alexithymia is a personality trait characterized by a diminished ability to identify and describe feelings, as well as an inability to distinguish physical symptoms associated with emotional arousal. Alexithymia is elevated in both patients with epilepsy (a neurologically-based seizure disorder) and psychogenic nonepileptic seizures (PNES; a psychological condition mimicking epilepsy); however, different neuropsychological processes may underlie this deficit in the two groups. To expand on previous research considering factors contributing to alexithymia in these populations, we examined the extent to which scores on the Toronto Alexithymia Scale (TAS-20) were predicted by performance on measures of executive and language functioning. We studied 138 PNES and 150 epilepsy patients with video-EEG confirmed diagnoses. Neuropsychological tests were administered to assess executive functioning (interference scores of the Stroop Color-Word Test and Part B of the Trail Making Test) and language functioning (Animals, Controlled Oral Word Association Test, and Boston Naming Test). Hierarchical linear regressions revealed that the relationships between disparate neuropsychological domains and alexithymia were not moderated by diagnosis of PNES or epilepsy. Multiple regression analyses within each group demonstrated that phonemic verbal fluency and response inhibition were significant predictors of alexithymia in epilepsy. Thus, alexithymia may reflect impairments in language and aspects of executive functioning in both PNES and epilepsy.
ContributorsReynolds, Christopher Martin (Author) / Roberts, Nicole A. (Thesis advisor) / Burleson, Mary H (Committee member) / Nanez, Jose (Committee member) / Arizona State University (Publisher)
Created2017