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- Genre: Masters Thesis
- Member of: Theses and Dissertations
Description
Bacteroides have been suggested as alternative indicators of fecal pollution since they are highly abundant in feces and are thought to have limited potential to grow in environment. However, recent literature suggests that Bacteroides can potentially survive within water distribution systems. The first objective of this study was therefore to investigate the validity of Bacteroides as a fecal indicator for drinking water through laboratory experiments and field studies. Experiments were performed using a laboratory scale PVC model water distribution system that was spiked with 109 Bacteroides. Samples were collected over the following four and analyzed by culture and molecular-based techniques. Second, field studies were performed by collecting water meters from two large chlorinated water distribution systems in central Arizona. Upon removal for repair by city personnel, meters were collected and biofilms samples were gathered within two hours. The biofilms were then analyzed using culture and molecular-based assays. The results from these studies support the hypothesis that Bacteroides DNA may be found in water distribution systems despite the difficulty of cultivating these bacterial cells. These experiments present the importance of considering biofilm interactions with fecal indicator bacteria when performing molecular assays on environmental samples, as biofilms may provide protection from high oxygen concentrations and grazing protozoa in bulk water that limit the persistence Bacteroides in the environment. Although the significance of biofilm interactions with surface or recreational waters may be small, they are likely important when considering drinking water delivered through distribution systems. The second objective of this study was to investigate alternative detection methodologies for the fecal indicator Bacteroides. In particular, this study focused on using a simplified protocol of Nucleic Acid Sequence Based Amplification (NASBA) and Thermophilic Helicase-Dependent Amplification (tHDA) to amplify the highly conserved 16s rRNA gene in the genomic DNA of fecal indicator Bacteroides. The results of this study show that the simplified NASBA procedure was not able to amplify the target, while continuous problems with tHDA exposed the methods lack of reliability. These results suggest higher reliability in the isothermal amplification methods needs to be achieved before application to environmental samples.
ContributorsDunkin, Nate (Author) / Abbaszadegan, Morteza (Thesis advisor) / Alum, Absar (Committee member) / Fox, Peter (Committee member) / Arizona State University (Publisher)
Created2012
Description
Ecolabels are the main driving force of consumer knowledge in the realm of sustainable product purchasing. While ecolabels strive to improve consumer's purchasing decisions, they have overwhelmed the market, leaving consumers confused and distrustful of what each label means. This study attempts to validate and understand environmental concerns commonly found in ecolabel criteria and the implications they have within the life cycle of a product. A life cycle assessment (LCA) case study of cosmetic products is used in comparison with current ecolabel program criteria to assess whether or not ecolabels are effectively driving environmental improvements in high impact areas throughout the life cycle of a product. Focus is placed on determining the general issues addressed by ecolabelling criteria and how these issues relate to hotspots derived through a practiced scientific methodology. Through this analysis, it was determined that a majority the top performing supply chain environmental impacts are covered, in some fashion, within ecolabelling criteria, but some, such as agricultural land occupation, are covered to a lesser extent or not at all. Additional criteria are suggested to fill the gaps found in ecolabelling programs and better address the environmental impacts most pertinent to the supply chain. Ecolabels have also been found to have a broader coverage then what can currently be addressed using LCA. The results of this analysis have led to a set of recommendations for furthering the integration between ecolabels and life cycle tools.
ContributorsBernardo, Melissa (Author) / Dooley, Kevin (Thesis advisor) / Chester, Mikhail (Thesis advisor) / Fox, Peter (Committee member) / Arizona State University (Publisher)
Created2012
Analysis and modeling of residual compounds in process streams from U.S. wastewater treatment plants
Description
The presence of compounds such as pharmaceuticals and personal care products (PPCPs) in the environment is a cause for concern as they exhibit secondary effects on non-target organisms and are also indicative of incomplete removal by wastewater treatment plants (WWTPs) during water reclamation. Analytical methods and predictive models can help inform on the rates at which these contaminants enter the environment via biosolids use or wastewater effluent release to estimate the risk of adverse effects. The goals of this research project were to integrate the results obtained from the two different methods of risk assessment, (a) in silico modeling and (b) experimental analysis. Using a previously published empirical model, influent and effluent concentration ranges were predicted for 10 sterols and validated with peer-reviewed literature. The in silico risk assessment analysis performed for sterols and hormones in biosolids concluded that hormones possess high leaching potentials and that particularly 17-α-ethinyl estradiol (EE2) can pose significant threat to fathead minnows (P. promelas) via leaching from terrestrial depositions of biosolids. Six mega-composite biosolids samples representative of 94 WWTPs were analyzed for a suite of 120 PPCPs using the extended U.S. EPA Method 1694 protocol. Results indicated the presence of 26 previously unmonitored PPCPs in the samples with estimated annual release rates of 5-15 tons yr-1 via land application of biosolids. A mesocosm sampling analysis that was included in the study concluded that four compounds amitriptyline, paroxetine, propranolol and sertraline warrant further monitoring due to their high release rates from land applied biosolids and their calculated extended half-lives in soils. There is a growing interest in the scientific community towards the development of new analytical protocols for analyzing solid matrices such as biosolids for the presence of PPCPs and other established and emerging contaminants of concern. The two studies presented here are timely and an important addition to the increasing base of scientific articles regarding environmental release of PPCPs and exposure risks associated with biosolids land application. This research study emphasizes the need for coupling experimental results with predictive analytical modeling output in order to more fully assess the risks posed by compounds detected in biosolids.
ContributorsPrakash Chari, Bipin (Author) / Halden, Rolf U. (Thesis advisor) / Westerhoff, Paul (Committee member) / Fox, Peter (Committee member) / Arizona State University (Publisher)
Created2012
Description
The purpose of this study was to determine the applicability of fluorescent microspheres as a surrogate to measure the removal of Cryptosporidium oocysts through the coagulation, flocculation, sedimentation, and filtration steps of conventional water treatment. In order to maintain accuracy and applicability, a local water treatment facility was chosen as the system to model. The city of Chandler Arizona utilizes conventional treatment methodologies to remove pathogens from municipal drinking water and thus the water, coagulant, polymer, and doses concentrations were sourced directly from the plant. Jar testing was performed on four combinations of coagulant, polymer, and fluorescent microsphere to determine if the log removal was similar to that of Cryptosporidium oocysts.
Complications with the material properties of the microspheres arose during testing that ultimately yielded unfavorable but conclusive results. Log removal of microspheres did not increase with added coagulant in the predicted manner, though the beads were seen aggregating, the low density of the particles made the sedimentation step inefficient. This result can be explained by the low density of the microspheres as well as the potential presence of residual coagulant present in the system. Given the unfavorable properties of the beads, they do not appear to be a suitable candidate for the surrogacy of Cryptosporidium oocysts in conventional drinking water treatment. The beads in their current state are not an adequate surrogate; however, future testing has been outlined to modify the experiment in such a way that the microspheres should behave like oocysts in terms of physical transportation.
Complications with the material properties of the microspheres arose during testing that ultimately yielded unfavorable but conclusive results. Log removal of microspheres did not increase with added coagulant in the predicted manner, though the beads were seen aggregating, the low density of the particles made the sedimentation step inefficient. This result can be explained by the low density of the microspheres as well as the potential presence of residual coagulant present in the system. Given the unfavorable properties of the beads, they do not appear to be a suitable candidate for the surrogacy of Cryptosporidium oocysts in conventional drinking water treatment. The beads in their current state are not an adequate surrogate; however, future testing has been outlined to modify the experiment in such a way that the microspheres should behave like oocysts in terms of physical transportation.
ContributorsLinks, Alexander Glenn (Author) / Abbaszadegan, Morteza (Thesis advisor) / Alum, Absar (Committee member) / Fox, Peter (Committee member) / Arizona State University (Publisher)
Created2015
Description
This study reports on benzene and toluene biodegradation under different dissolved oxygen conditions, and the goal of this study is to evaluate and model their removal.
Benzene and toluene were tested for obligate anaerobic degradation in batch reactors with sulfate as the electron acceptor. A group of sulfate-reducing bacteria capable of toluene degradation was enriched after 252 days of incubation. Those cultures, originated from anaerobic digester, were able to degrade toluene coupled to sulfate reduction with benzene coexistence, while they were not able to utilize benzene. Methanogens also were present, although their contribution to toluene biodegradation was not defined.
Aerobic biodegradation of benzene and toluene by Pseudomonas putida F1 occurred, and biomass production lagged behind substrate loss and continued after complete substrate removal. This pattern suggests that biodegradation of intermediates, rather than direct benzene and toluene transformation, caused bacterial growth. Supporting this explanation is that the calculated biomass growth from a two-step model basically fit the experimental biomass results during benzene and toluene degradation with depleted dissolved oxygen.
Catechol was tested for anaerobic biodegradation in batch experiments and in a column study. Sulfate- and nitrate-reducing bacteria enriched from a wastewater treatment plant hardly degraded catechol within 20 days. However, an inoculum from a contaminated site was able to remove 90% of the initial 16.5 mg/L catechol, and Chemical Oxygen Demand was oxidized in parallel. Catechol biodegradation was inhibited when nitrite accumulated, presumably by a toxic catechol-nitrite complex.
The membrane biofilm reactor (MBfR) offers the potential for biodegrading benzene in a linked aerobic and anaerobic pathway by controlling the O2 delivery. At an average benzene surface loading of 1.3 g/m2-day and an average hydraulic retention time of 2.2 day, an MBfR supplied with pure O2 successfully achieved 99% benzene removal at steady state. A lower oxygen partial pressure led to decreased benzene removal, and nitrate removal increased, indicating multiple mechanisms, including oxygenation and nitrate reduction, were involved in the system being responsible for benzene removal. Microbial community analysis indicated that Comamonadaceae, a known aerobic benzene-degrader and denitrifier, dominated the biofilm at the end of operation.
Benzene and toluene were tested for obligate anaerobic degradation in batch reactors with sulfate as the electron acceptor. A group of sulfate-reducing bacteria capable of toluene degradation was enriched after 252 days of incubation. Those cultures, originated from anaerobic digester, were able to degrade toluene coupled to sulfate reduction with benzene coexistence, while they were not able to utilize benzene. Methanogens also were present, although their contribution to toluene biodegradation was not defined.
Aerobic biodegradation of benzene and toluene by Pseudomonas putida F1 occurred, and biomass production lagged behind substrate loss and continued after complete substrate removal. This pattern suggests that biodegradation of intermediates, rather than direct benzene and toluene transformation, caused bacterial growth. Supporting this explanation is that the calculated biomass growth from a two-step model basically fit the experimental biomass results during benzene and toluene degradation with depleted dissolved oxygen.
Catechol was tested for anaerobic biodegradation in batch experiments and in a column study. Sulfate- and nitrate-reducing bacteria enriched from a wastewater treatment plant hardly degraded catechol within 20 days. However, an inoculum from a contaminated site was able to remove 90% of the initial 16.5 mg/L catechol, and Chemical Oxygen Demand was oxidized in parallel. Catechol biodegradation was inhibited when nitrite accumulated, presumably by a toxic catechol-nitrite complex.
The membrane biofilm reactor (MBfR) offers the potential for biodegrading benzene in a linked aerobic and anaerobic pathway by controlling the O2 delivery. At an average benzene surface loading of 1.3 g/m2-day and an average hydraulic retention time of 2.2 day, an MBfR supplied with pure O2 successfully achieved 99% benzene removal at steady state. A lower oxygen partial pressure led to decreased benzene removal, and nitrate removal increased, indicating multiple mechanisms, including oxygenation and nitrate reduction, were involved in the system being responsible for benzene removal. Microbial community analysis indicated that Comamonadaceae, a known aerobic benzene-degrader and denitrifier, dominated the biofilm at the end of operation.
ContributorsLiu, Zhuolin (Author) / Rittmann, Bruce E. (Thesis advisor) / Krajmalnik-Brown, Rosa (Committee member) / Fox, Peter (Committee member) / Arizona State University (Publisher)
Created2015
Description
Quagga Mussels (Dreissena bugensis) are an invasive species of mollusk that have established themselves within the Colorado River system of Arizona since 2007. However, despite close proximity and frequent travel by recreational boaters between reservoirs, they have not yet infested the Salt River or Verde River systems. Laboratory experimentation was done to test the survival rate of adult D. bugensis specimens in waters collected from Bartlett Lake (Verde River), Saguaro Lake (Salt River), and Salt River Project (SRP) canals (Salt River/Verde River/Colorado River blend) as well as Central Arizona Project (CAP) canals with the addition of turbidity to simulate high runoff storm events. Under each condition, adult survival for a time period of 21 days exceeded 98%, ruling out water chemistry or turbidity as a factor. Spawning was investigated using mussels collected from Lake Pleasant in August 2015. In 4 trials of serotonin dosage between 0.5 – 1.0 mMol, spawning was not successful. Calanoid copepod predation was also investigated by field sampling at Lake Pleasant, Saguaro Lake, and Bartlett Lake during September 2015. Calanoid copepods were identified in Lake Pleasant at a density of 104.22 individuals per cubic meter at a depth of 2 meters and 9.75 individuals per cubic meter at the surface. Calanoid copepods were not found in Bartlett Lake or Saguaro Lake, ruling out copepod predation as a factor. Finally, dissolved oxygen and temperature trends were analyzed in each reservoir. While temperature profiles are similar throughout the year, seasonal drops in dissolved oxygen below survivable concentrations for D. bugensis has been observed in both Saguaro Lake and Bartlett Lake but not Lake Pleasant, representing the most plausible explanation for no observed infestation.
ContributorsSokolowski, Matthew (Author) / Fox, Peter (Thesis advisor) / Abbaszadegan, Morteza (Thesis advisor) / Alum, Absar (Committee member) / Arizona State University (Publisher)
Created2015
Description
Adenoviruses cause gastrointestinal illnesses and have been listed on the U.S. EPA’s Contaminant Candidate Lists (CCL). They are highly resistant to ultraviolet (UV) inactivation. Advanced oxidation processes (AOPs) are known to improve inactivation of microorganisms and simultaneously oxidize organics. The bacteriophage P22 was selected as a surrogate for adenoviruses due to their physical and genetic similarities.
The main objective of this study was to compare the synergic disinfection potential of titanium dioxide (TiO2) or peracetic acid (PAA) with UV for viruses and bacteria in water.
Both bench-scale and pilot-scale evaluation was done. A bench-scale collimated beam was included to evaluate the inactivation of P22 and E. coli by UV with and without TiO2 or PAA. A Purifics Photo-Cat system which is an integrated UV/ceramic membrane reactor was used for the pilot-scale TiO2-UV AOP experiments. For pilot-scale PAA-UV AOP experiments, an in-line D222 UV reactor unit provided by NeoTech Aqua Solutions, Inc. was used.
TiO2 doses of 1, 10, and 40 mg/L were applied in the collimated beam and the Photo-Cat system. Higher TiO2 doses resulted in a higher inactivation in the Photo-Cat and lower inactivation in the collimated beam apparatus. Adding 40 mg/L of TiO2 in the photo-Cat system improved P22 inactivation by 25% while it slightly decreased P22 inactivation in collimated beam apparatus.
PAA doses of 0.25 or 0.5 ppm were continuously injected upstream of the UV light and a 53% or 90% increase in inactivation was observed for E. coli, respectively, as compared to UV alone. However, P22 required higher dose with PAA-UV AOP and PAA concentrations of 1 or 10 ppm resulted in an 18% and 70% increase in the inactivation respectively, as compared to UV alone. Interestingly, when the same condition was applied to water with more organics (UVT 79%), E. coli exhibited the same level of susceptibility to PAA-UV AOP while P22 inactivation decreased.
The results provide new insight on the effectiveness and applicability of adding AOP to UV for microbial inactivation in water. PAA-UV AOP can potentially enhance existing UV disinfection systems with minimal chemical addition, and a simple retrofit to existing UV units.
The main objective of this study was to compare the synergic disinfection potential of titanium dioxide (TiO2) or peracetic acid (PAA) with UV for viruses and bacteria in water.
Both bench-scale and pilot-scale evaluation was done. A bench-scale collimated beam was included to evaluate the inactivation of P22 and E. coli by UV with and without TiO2 or PAA. A Purifics Photo-Cat system which is an integrated UV/ceramic membrane reactor was used for the pilot-scale TiO2-UV AOP experiments. For pilot-scale PAA-UV AOP experiments, an in-line D222 UV reactor unit provided by NeoTech Aqua Solutions, Inc. was used.
TiO2 doses of 1, 10, and 40 mg/L were applied in the collimated beam and the Photo-Cat system. Higher TiO2 doses resulted in a higher inactivation in the Photo-Cat and lower inactivation in the collimated beam apparatus. Adding 40 mg/L of TiO2 in the photo-Cat system improved P22 inactivation by 25% while it slightly decreased P22 inactivation in collimated beam apparatus.
PAA doses of 0.25 or 0.5 ppm were continuously injected upstream of the UV light and a 53% or 90% increase in inactivation was observed for E. coli, respectively, as compared to UV alone. However, P22 required higher dose with PAA-UV AOP and PAA concentrations of 1 or 10 ppm resulted in an 18% and 70% increase in the inactivation respectively, as compared to UV alone. Interestingly, when the same condition was applied to water with more organics (UVT 79%), E. coli exhibited the same level of susceptibility to PAA-UV AOP while P22 inactivation decreased.
The results provide new insight on the effectiveness and applicability of adding AOP to UV for microbial inactivation in water. PAA-UV AOP can potentially enhance existing UV disinfection systems with minimal chemical addition, and a simple retrofit to existing UV units.
ContributorsNikougoftar Zarif, Majid (Author) / Abbaszadegan, Morteza (Thesis advisor) / Fox, Peter (Committee member) / Conroy-Ben, Otakuye (Committee member) / Arizona State University (Publisher)
Created2017
Description
Legionella pneumophila is a waterborne pathogen that causes Legionnaires' disease, an infection which can lead to potentially fatal pneumonia. In a culture-based technique, Legionella is detected using buffered charcoal-yeast extract (BCYE) agar supplemented with L-cysteine, Iron salt and antibiotics. These supplements provide essential and complex nutrient requirements and help in the suppression of non-target bacteria in Legionella analysis. Legionella occurs naturally in freshwater environments and for their detection; a sample is plated on solid agar media and then incubated for several days. There are many challenges in the detection of Legionella in environmental waters and the built environments. A common challenge is that a variety of environmental bacteria can be presumptively identified as Legionella using the culture-based method. In addition, proper identification of Legionella requires long incubation period (3-9 days) while antibiotics used in BCYE agar have relatively short half-life time. In order to overcome some of the challenges, Legionella has been genetically modified to express reporter genes such Green Fluorescent Protein (GFP) that can facilitate its detection in process validation studies under controlled laboratory conditions. However, such studies had limited success due to the instability of genetically modified Legionella strains. The development of a genetically modified Legionella with a much rapid growth rate (1-2 days) in simulated environmental systems (tightly-controlled water distribution system) is achieved. The mutant Legionella is engineered by transforming with a specific plasmid encoding CymR, LacZ and TetR genes. The newly engineered Legionella can grow on conventional BCYE agar media without L-Cysteine, Iron salt and only require one antibiotic (Tetracycline) to suppress the growth of other microorganisms in media. To the best of our knowledge, this is the first report of L. pneumophila strain capable of growing without L-Cysteine. We believe that this discovery would not only facilitate the study of the fate and transport of this pathogen in environmental systems, but also further our understanding of the genetics and metabolic pathways of Legionella.
ContributorsAloraini, Saleh Ali A (Author) / Abbaszadegan, Morteza (Thesis advisor) / Fox, Peter (Committee member) / Alum, Absar (Committee member) / Arizona State University (Publisher)
Created2018
Description
The sensor industry is a growing industry that has been predicted by Allied Market Research to be a multi-billion industry by 2022. One of the many key drives behind this rapid growth in the sensor industry is the increase incorporation of sensors into portable electrical devices. The value for sensor technologies are increased when the sensors are developed into innovative measuring system for application uses in the Aerospace, Defense, and Healthcare industries. While sensors are not new, their increased performance, size reduction, and decrease in cost has opened the door for innovative sensor combination for portable devices that could be worn or easily moved around. With this opportunity for further development of sensor use through concept engineering development, three concept projects for possible innovative portable devices was undertaken in this research. One project was the development of a pulse oximeter devise with fingerprint recognition. The second project was prototyping a portable Bluetooth strain gage monitoring system. The third project involved sensors being incorporated onto flexible printed circuit board (PCB) for improved comfort of wearable devices. All these systems were successfully tested in lab.
ContributorsNichols, Kevin William (Author) / Redkar, Sangram (Thesis advisor) / Rogers, Brad (Committee member) / Sugar, Thomas (Committee member) / Arizona State University (Publisher)
Created2018
Description
Quagga mussels are an aquatic invasive species capable of causing economic and ecological damage. Despite the quagga mussels’ ability to rapidly spread, two watersheds, the Salt River system and the Verde River system of Arizona, both had no quagga mussel detections for 8 years. The main factor thought to deter quagga mussels was the stratification of the two watersheds during the summer, resulting in high temperatures in the epilimnion and low dissolved oxygen in the hypolimnion. In 2015, Canyon Lake, a reservoir of the Salt River watershed, tested positive for quagga mussel veligers. In this study, I used Landsat 7 and Landsat 8 satellite data to determine if changes in the surface temperature have caused a change to the reservoir allowing quagga mussel contamination. I used a location in the center of the lake with a root mean squared error (RMSE) of 0.80 and a correlation coefficient (R^2) of 0.82, but I did not detect any significant variations in surface temperatures from recent years. I also measured 21 locations on Canyon Lake to determine if the locations in Canyon Lake were able to harbor quagga mussels. I found that summer stratification caused hypolimnion dissolved oxygen levels to drop well below the quagga mussel threshold of 2mg/L. Surface temperatures, however were not high enough throughout the lake to prevent quagga mussels from inhabiting the epilimnion. It is likely that a lack of substrate in the epilimnion have forced any quagga mussel inhabitants in Canyon Lake to specific locations that were not necessarily near the point of quagga veliger detection sampling. The research suggests that while Canyon Lake may have been difficult for quagga mussels to infest, once they become established in the proper locations, where they can survive through the summer, quagga mussels are likely to become more prevalent.
ContributorsLau, Theresa (Author) / Fox, Peter (Thesis advisor) / Neuer, Susanne (Committee member) / Abbaszadegan, Morteza (Committee member) / Arizona State University (Publisher)
Created2018