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- Genre: Doctoral Dissertation
Description
ABSTRACT Peptide microarrays may prove to be a powerful tool for proteomics research and clinical diagnosis applications. Fodor et al. and Maurer et al. have shown proof-of-concept methods of light- and electrochemically-directed peptide microarray fabrication on glass and semiconductor microchips respectively. In this work, peptide microarray fabrication based on the abovementioned techniques were optimized. In addition, MALDI mass spectrometry based peptide synthesis characterization on semiconductor microchips was developed and novel applications of a CombiMatrix (CBMX) platform for electrochemically controlled synthesis were explored. We have investigated performance of 2-(2-nitrophenyl)propoxycarbonyl (NPPOC) derivatives as photo-labile protecting group. Specifically, influence of substituents on 4 and 5 positions of phenyl ring of NPPOC group on the rate of photolysis and the yield of the amine was investigated. The results indicated that substituents capable of forming a π-network with the nitro group enhanced the rate of photolysis and yield. Once such properly substituted NPPOC groups were used, the rate of photolysis/yield depended on the nature of protected amino group indicating that a different chemical step during the photo-cleavage process became the rate limiting step. We also focused on electrochemically-directed parallel synthesis of high-density peptide microarrays using the CBMX technology referred to above which uses electrochemically generated acids to perform patterned chemistry. Several issues related to peptide synthesis on the CBMX platform were studied and optimized, with emphasis placed on the reactions of electro-generated acids during the deprotection step of peptide synthesis. We have developed a MALDI mass spectrometry based method to determine the chemical composition of microarray synthesis, directly on the feature. This method utilizes non-diffusional chemical cleavage from the surface, thereby making the chemical characterization of high-density microarray features simple, accurate, and amenable to high-throughput. CBMX Corp. has developed a microarray reader which is based on electro-chemical detection of redox chemical species. Several parameters of the instrument were studied and optimized and novel redox applications of peptide microarrays on CBMX platform were also investigated using the instrument. These include (i) a search of metal binding catalytic peptides to reduce overpotential associated with water oxidation reaction and (ii) an immobilization of peptide microarrays using electro-polymerized polypyrrole.
ContributorsKumar, Pallav (Author) / Woodbury, Neal (Thesis advisor) / Allen, James (Committee member) / Johnston, Stephen (Committee member) / Arizona State University (Publisher)
Created2013
Description
The Chinese Construction Industry has grown to be one of the largest construction markets in the world within the last 10 years. The size of the Chinese Construction Industry is on par with many developed nations, despite it being a developing country. Despite its rapid growth, the productivity and profitability of the Chinese Construction Industry is low compared to similar sized construction industries (United States, United Kingdom, etc.). In addition to the low efficiency of the Chinese Construction Industry, there is minimal documentation available showing the performance of the Chinese Construction Industry (projects completed on time, on budget, and customer satisfaction ratings).
The purpose of this research is to investigate potential solutions that could address the poor efficiency and performance of the Chinese Construction Industry. This research is divided into three phases; first, a literature review to identify countries that have similar construction industries to the Chinese Construction Industry. The second phase is to compare the risks and identify solutions that are proposed to increase the performance of similar construction industries and the Chinese Construction Industry. The third phase is to create a survey from the literature-based information to validate the concepts with the Chinese Construction Industry professionals and stakeholders.
The purpose of this research is to investigate potential solutions that could address the poor efficiency and performance of the Chinese Construction Industry. This research is divided into three phases; first, a literature review to identify countries that have similar construction industries to the Chinese Construction Industry. The second phase is to compare the risks and identify solutions that are proposed to increase the performance of similar construction industries and the Chinese Construction Industry. The third phase is to create a survey from the literature-based information to validate the concepts with the Chinese Construction Industry professionals and stakeholders.
ContributorsChen, Yutian (Author) / Chong, Oswald (Thesis advisor) / Kashiwagi, Dean T. (Committee member) / Badger, Willliam (Committee member) / Arizona State University (Publisher)
Created2020
Description
Particulate Guanylyl Cyclase Receptor A (pGC-A) is an atrial natriuretic peptide receptor, which plays a vital role in controlling cardiovascular, renal, and endocrine functions. The extracellular domain of pGC-A interacts with natriuretic peptides and triggers the intracellular guanylyl cyclase domain to convert GTP to cGMP. To effectively develop a method that can regulate pGC-A, structural information regarding its intact form is necessary. Currently, only the extracellular domain structure of rat pGC-A has been determined. However, structural data regarding the transmembrane domain, as well as functional intracellular domain regions, need to be elucidated.This dissertation presents detailed information regarding pGC-A expression and optimization in the baculovirus expression vector system, along with the first purification method for purifying functional intact human pGC-A. The first in vitro evidence of a purified intact human pGC-A tetramer was detected in detergent micellar solution. Intact pGC-A is currently proposed to function as a homodimer. Upon analyzing my findings and acknowledging that dimer formation is required for pGC-A functionality, I proposed the first tetramer complex model composed of two functional subunits (homodimer). Forming tetramer complexes on the cell membrane increases pGC-A binding efficiency and ligand sensitivity.
Currently, a two-step mechanism has been proposed for ATP-dependent pGC-A signal transduction. Based on cGMP functional assay results, it can be suggested that the binding ligand also moderately activates pGC-A, and that ATP is not crucial for the activation of guanylyl cyclase. Instead, three modulators can regulate different activation levels in intact pGC-A.
Crystallization of purified intact pGC-A was performed to determine its structure. During the crystallization condition screening process, I successfully selected seven promising initial crystallization conditions for intact human pGC-A crystallization. One selected condition led to the formation of excellent needle-shaped crystals. During the serial crystallography diffraction experiment, five diffraction patterns were detected. The highest diffraction resolution spot reached 3 Å.
This work will allow the determination of the intact human pGC-A structure while also providing structural information on the protein signal transduction mechanism. Further structural knowledge may potentially lead to improved drug design. More precise mutation experiments could help verify the current pGC-A signal transduction and activation mechanism.
ContributorsZhang, Shangji (Author) / Fromme, Petra (Thesis advisor) / Johnston, Stephen (Committee member) / Mazor, Yuval (Committee member) / Arizona State University (Publisher)
Created2021
Description
The consequences of failures from large-diameter water pipelines can be severe. Results can include significant property damage, damage to adjacent infrastructure such as roads and bridges resulting in transportation delays or shutdowns, adjacent structural damage to buildings resulting in loss of business, service disruption to a significant number of customers, loss of water, costly emergency repairs, and even loss of life. The American Water Works Association’s (AWWA) 2020 “State of the Water Industry” report states the top issue facing the water industry since 2016 is aging infrastructure, with the second being financing for improvements. The industry must find innovative ways to extend asset life and reduce maintenance expenditures. While are many different assets comprise the drinking water industry, pipelines are a major component and often neglected because they are typically buried. Reliability Centered Maintenance (RCM) is a process used to determine the most effective maintenance strategy for an asset, with the ultimate goal being to establish the required function of the asset with the required reliability at the lowest operations and maintenance costs. The RCM philosophy considers Preventive Maintenance, Predictive Maintenance, Condition Based Monitoring, Reactive Maintenance, and Proactive Maintenance techniques in an integrated manner to increase the probability an asset will perform its designed function throughout its design life with minimal maintenance. In addition to determining maintenance tasks, the timely performance of those tasks is crucial. If performed too late an asset may fail; if performed too early, resources that may be used better elsewhere are expended. Utility agencies can save time and money by using RCM analysis for their drinking water infrastructure. This dissertation reviews industries using RCM, discusses the benefits of an RCM analysis, and goes through a case study of an RCM at a large aqueduct in the United States. The dissertation further discusses the consequence of failure of large diameter water pipelines and proposes a regression model to help agencies determine the optimum time to perform maintenance tasks on large diameter prestressed concrete pipelines using RCM analysis.
ContributorsGeisbush, James R (Author) / Ariaratnam, Samuel T (Thesis advisor) / Grau, David (Committee member) / Chong, Oswald (Committee member) / Arizona State University (Publisher)
Created2024
Description
The United States building sector was the most significant carbon emission contributor (over 40%). The United States government is trying to decrease carbon emissions by enacting policies, but emissions increased by approximately 7 percent in the U.S. between 1990 and 2013. To reduce emissions, investigating the factors affecting carbon emissions should be a priority. Therefore, in this dissertation, this research examine the relationship between carbon emissions and the factors affecting them from macro and micro perspectives. From a macroscopic perspective, the relationship between carbon dioxide, energy resource consumption, energy prices, GDP (gross domestic product), waste generation, and recycling waste generation in the building and waste sectors has been verified. From a microscopic perspective, the impact of non-permanent electric appliances and stationary and non-stationary occupancy has been investigated. To verify the relationships, various kinds of statistical and data mining techniques were applied, such as the Granger causality test, linear and logarithmic correlation, and regression method. The results show that natural gas and electricity prices are higher than others, as coal impacts their consumption, and electricity and coal consumption were found to cause significant carbon emissions. Also, waste generation and recycling significantly increase and decrease emissions from the waste sector, respectively. Moreover, non-permanent appliances such as desktop computers and monitors consume a lot of electricity, and significant energy saving potential has been shown. Lastly, a linear relationship exists between buildings’ electricity use and total occupancy, but no significant relationship exists between occupancy and thermal loads, such as cooling and heating loads. These findings will potentially provide policymakers with a better understanding of and insights into carbon emission manipulation in the building sector.
ContributorsLee, Seungtaek (Author) / Chong, Oswald (Thesis advisor) / Sullivan, Kenneth (Committee member) / Tang, Pingbo (Committee member) / Arizona State University (Publisher)
Created2018
Description
As a developing nation, China is currently faced with the challenge of providing
safe, reliable and adequate energy resources to the county's growing urban areas as well as to its expanding rural populations. To meet this demand, the country has initiated massive construction projects to expand its national energy infrastructure, particularly in the form of natural gas pipeline. The most notable of these projects is the ongoing West-East Gas Pipeline Project. This project is currently in its third phase, which will supply clean and efficient natural gas to nearly sixty million users located in the densely populated Yangtze River Delta.
Trenchless Technologies, in particular the construction method of Horizontal
Directional Drilling (HDD), have played a critical role in executing this project by
providing economical, practical and environmentally responsible ways to install buried pipeline systems. HDD has proven to be the most popular method selected to overcome challenges along the path of the pipeline, which include mountainous terrain, extensive farmland and numerous bodies of water. The Yangtze River, among other large-scale water bodies, have proven to be the most difficult obstacle for the pipeline installation as it widens and changes course numerous times along its path to the East China Sea. The purpose of this study is to examine those practices being used in China in order to compare those to those long used practices in the North American in order to understand the advantages of Chinese advancements.
Developing countries would benefit from the Chinese advancements for large-scale HDD installation. In developed areas, such as North America, studying Chinese execution may allow for new ideas to help to improve long established methods. These factors combined further solidify China's role as the global leader in trenchless technology methods and provide the opportunity for Chinese HDD contractors to contribute to the world's knowledge for best practices of the Horizontal Directional Drilling method.
safe, reliable and adequate energy resources to the county's growing urban areas as well as to its expanding rural populations. To meet this demand, the country has initiated massive construction projects to expand its national energy infrastructure, particularly in the form of natural gas pipeline. The most notable of these projects is the ongoing West-East Gas Pipeline Project. This project is currently in its third phase, which will supply clean and efficient natural gas to nearly sixty million users located in the densely populated Yangtze River Delta.
Trenchless Technologies, in particular the construction method of Horizontal
Directional Drilling (HDD), have played a critical role in executing this project by
providing economical, practical and environmentally responsible ways to install buried pipeline systems. HDD has proven to be the most popular method selected to overcome challenges along the path of the pipeline, which include mountainous terrain, extensive farmland and numerous bodies of water. The Yangtze River, among other large-scale water bodies, have proven to be the most difficult obstacle for the pipeline installation as it widens and changes course numerous times along its path to the East China Sea. The purpose of this study is to examine those practices being used in China in order to compare those to those long used practices in the North American in order to understand the advantages of Chinese advancements.
Developing countries would benefit from the Chinese advancements for large-scale HDD installation. In developed areas, such as North America, studying Chinese execution may allow for new ideas to help to improve long established methods. These factors combined further solidify China's role as the global leader in trenchless technology methods and provide the opportunity for Chinese HDD contractors to contribute to the world's knowledge for best practices of the Horizontal Directional Drilling method.
ContributorsCarlin, Maureen Cassin (Author) / Ariaratnam, Samuel T (Thesis advisor) / Chong, Oswald (Committee member) / Bearup, Wylie (Committee member) / Arizona State University (Publisher)
Created2014
Description
The solar energy sector has been growing rapidly over the past decade. Growth in renewable electricity generation using photovoltaic (PV) systems is accompanied by an increased awareness of the fault conditions developing during the operational lifetime of these systems. While the annual energy losses caused by faults in PV systems could reach up to 18.9% of their total capacity, emerging technologies and models are driving for greater efficiency to assure the reliability of a product under its actual application. The objectives of this dissertation consist of (1) reviewing the state of the art and practice of prognostics and health management for the Direct Current (DC) side of photovoltaic systems; (2) assessing the corrosion of the driven posts supporting PV structures in utility scale plants; and (3) assessing the probabilistic risk associated with the failure of polymeric materials that are used in tracker and fixed tilt systems.
As photovoltaic systems age under relatively harsh and changing environmental conditions, several potential fault conditions can develop during the operational lifetime including corrosion of supporting structures and failures of polymeric materials. The ability to accurately predict the remaining useful life of photovoltaic systems is critical for plants ‘continuous operation. This research contributes to the body of knowledge of PV systems reliability by: (1) developing a meta-model of the expected service life of mounting structures; (2) creating decision frameworks and tools to support practitioners in mitigating risks; (3) and supporting material selection for fielded and future photovoltaic systems. The newly developed frameworks were validated by a global solar company.
As photovoltaic systems age under relatively harsh and changing environmental conditions, several potential fault conditions can develop during the operational lifetime including corrosion of supporting structures and failures of polymeric materials. The ability to accurately predict the remaining useful life of photovoltaic systems is critical for plants ‘continuous operation. This research contributes to the body of knowledge of PV systems reliability by: (1) developing a meta-model of the expected service life of mounting structures; (2) creating decision frameworks and tools to support practitioners in mitigating risks; (3) and supporting material selection for fielded and future photovoltaic systems. The newly developed frameworks were validated by a global solar company.
ContributorsChokor, Abbas (Author) / El Asmar, Mounir (Thesis advisor) / Chong, Oswald (Committee member) / Ernzen, James (Committee member) / Arizona State University (Publisher)
Created2017
Description
Civil infrastructures undergo frequent spatial changes such as deviations between as-designed model and as-is condition, rigid body motions of the structure, and deformations of individual elements of the structure, etc. These spatial changes can occur during the design phase, the construction phase, or during the service life of a structure. Inability to accurately detect and analyze the impact of such changes may miss opportunities for early detections of pending structural integrity and stability issues. Commercial Building Information Modeling (BIM) tools could hardly track differences between as-designed and as-built conditions as they mainly focus on design changes and rely on project managers to manually update and analyze the impact of field changes on the project performance. Structural engineers collect detailed onsite data of a civil infrastructure to perform manual updates of the model for structural analysis, but such approach tends to become tedious and complicated while handling large civil infrastructures.
Previous studies started collecting detailed geometric data generated by 3D laser scanners for defect detection and geometric change analysis of structures. However, previous studies have not yet systematically examined methods for exploring the correlation between the detected geometric changes and their relation to the behaviors of the structural system. Manually checking every possible loading combination leading to the observed geometric change is tedious and sometimes error-prone. The work presented in this dissertation develops a spatial change analysis framework that utilizes spatiotemporal data collected using 3D laser scanning technology and the as-designed models of the structures to automatically detect, classify, and correlate the spatial changes of a structure. The change detection part of the developed framework is computationally efficient and can automatically detect spatial changes between as-designed model and as-built data or between two sets of as-built data collected using 3D laser scanning technology. Then a spatial change classification algorithm automatically classifies the detected spatial changes as global (rigid body motion) and local deformations (tension, compression). Finally, a change correlation technique utilizes a qualitative shape-based reasoning approach for identifying correlated deformations of structure elements connected at joints that contradicts the joint equilibrium. Those contradicting deformations can help to eliminate improbable loading combinations therefore guiding the loading path analysis of the structure.
Previous studies started collecting detailed geometric data generated by 3D laser scanners for defect detection and geometric change analysis of structures. However, previous studies have not yet systematically examined methods for exploring the correlation between the detected geometric changes and their relation to the behaviors of the structural system. Manually checking every possible loading combination leading to the observed geometric change is tedious and sometimes error-prone. The work presented in this dissertation develops a spatial change analysis framework that utilizes spatiotemporal data collected using 3D laser scanning technology and the as-designed models of the structures to automatically detect, classify, and correlate the spatial changes of a structure. The change detection part of the developed framework is computationally efficient and can automatically detect spatial changes between as-designed model and as-built data or between two sets of as-built data collected using 3D laser scanning technology. Then a spatial change classification algorithm automatically classifies the detected spatial changes as global (rigid body motion) and local deformations (tension, compression). Finally, a change correlation technique utilizes a qualitative shape-based reasoning approach for identifying correlated deformations of structure elements connected at joints that contradicts the joint equilibrium. Those contradicting deformations can help to eliminate improbable loading combinations therefore guiding the loading path analysis of the structure.
ContributorsKalasapudi, Vamsi Sai (Author) / Tang, Pingbo (Thesis advisor) / Chong, Oswald (Committee member) / Hjelmstad, Keith (Committee member) / Arizona State University (Publisher)
Created2017
Description
Immunosignature is a technology that retrieves information from the immune system. The technology is based on microarrays with peptides chosen from random sequence space. My thesis focuses on improving the Immunosignature platform and using Immunosignatures to improve diagnosis for diseases. I first contributed to the optimization of the immunosignature platform by introducing scoring metrics to select optimal parameters, considering performance as well as practicality. Next, I primarily worked on identifying a signature shared across various pathogens that can distinguish them from the healthy population. I further retrieved consensus epitopes from the disease common signature and proposed that most pathogens could share the signature by studying the enrichment of the common signature in the pathogen proteomes. Following this, I worked on studying cancer samples from different stages and correlated the immune response with whether the epitope presented by tumor is similar to the pathogen proteome. An effective immune response is defined as an antibody titer increasing followed by decrease, suggesting elimination of the epitope. I found that an effective immune response usually correlates with epitopes that are more similar to pathogens. This suggests that the immune system might occupy a limited space and can be effective against only certain epitopes that have similarity with pathogens. I then participated in the attempt to solve the antibiotic resistance problem by developing a classification algorithm that can distinguish bacterial versus viral infection. This algorithm outperforms other currently available classification methods. Finally, I worked on the concept of deriving a single number to represent all the data on the immunosignature platform. This is in resemblance to the concept of temperature, which is an approximate measurement of whether an individual is healthy. The measure of Immune Entropy was found to work best as a single measurement to describe the immune system information derived from the immunosignature. Entropy is relatively invariant in healthy population, but shows significant differences when comparing healthy donors with patients either infected with a pathogen or have cancer.
ContributorsWang, Lu (Author) / Johnston, Stephen (Thesis advisor) / Stafford, Phillip (Committee member) / Buetow, Kenneth (Committee member) / McFadden, Grant (Committee member) / Arizona State University (Publisher)
Created2018
Description
Regulatory agencies, such as the Occupational Safety and Health Administration (OSHA), and the National Institute of Occupational Safety and Health (NIOSH), recognize that decisions regarding occupational health are often economically driven, with worker health only a secondary concern (Ruttenberg, 2014). To investigate the four National Occupational Research Agenda (NORA) long-standing health concerns—welding fumes, crystalline silica, noise, and musculoskeletal disorders—a mixed methods research is conducted. Fourfold structuration, a holistic communication process with roots in indigenous/ancient knowledge, is used to organize data and facilitate making tangible relationships of health to productivity and profits that are abstract and often stated by industries, such as construction, as difficult to quantify. From both construction trade worker and occupational health and safety expert interviews data/codes are developed. For the qualitative method, the codes are organized into a constructivist grounded theory depicting the construction industry with regard to its foundation – profits. A theoretical exercise translating the qualitative codes into potential productivity losses is presented as a way for quantifying the abstract relationships of health to productivity. For the quantitative study, the data/codes are used to develop a comprehensive list of practices, barriers to, and catalysts for addressing health in construction. A significant quantitative finding is that occupational health and safety (OSH) experts are not traditionally involved at the highest levels of the OSHA Hierarchy of Controls, where the greatest opportunity to prevent exposure to health hazards is possible. Organized via a holistic framework, this research emphasizes our primary responsibility to each other as highlighted in recent NIOSH worker health agendas.
ContributorsTello, Linda Marguerite (Author) / Grau, David (Thesis advisor) / Koro-Ljungberg, Mirka (Committee member) / Hanemann, Michael (Committee member) / Chong, Oswald (Committee member) / Arizona State University (Publisher)
Created2017