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Description
Coccidioidomycosis, also known as Valley Fever, is a disease caused by the dimorphic soil-dwelling fungus, Coccidioides sp. Coccidioidomycosis is difficult to diagnose because symptoms are similar to community-acquired pneumonia. Current diagnostic tests rely on antibody responses, but immune responses can be delayed and aberrant, resulting in false negative diagnoses. Unlike

Coccidioidomycosis, also known as Valley Fever, is a disease caused by the dimorphic soil-dwelling fungus, Coccidioides sp. Coccidioidomycosis is difficult to diagnose because symptoms are similar to community-acquired pneumonia. Current diagnostic tests rely on antibody responses, but immune responses can be delayed and aberrant, resulting in false negative diagnoses. Unlike serology, detection of coccidioidal proteins or other fungal components in blood could distinguish valley fever from other pulmonary infections and provide a definitive diagnosis. Using mass spectrometry (LC-MS/MS) we examined the plasma peptidome from patients with serologically confirmed coccidioidomycosis. Mass spectra were searched using the protein database from the Coccidioides species, generated and annotated by the Broad Institute. 15 of 20 patients with serologically confirmed coccidioidomycosis demonstrated the presence of a peptide in plasma, "PGLDSKSLACTFSQV" (PGLD). The peptide is derived from an open reading frame from a "conserved hypothetical protein" annotated with 2 exons, and to date, found only in the C. posadasii strain Silviera RMSCC 3488 genomic sequence. In this thesis work, cDNA sequence analysis from polyadenylated RNA confirms the peptide sequence and genomic location of the peptide, but does not indicate that the intron in the gene prediction of C. posadasii strain Silviera RMSCC 3488 is present. A monoclonal antibody generated against the peptide bound to a 16kDa protein in T27K coccidioidal lysate. Detecting components of the fungus plasma could be a useful diagnostic tool, especially when serology does not provide a definitive diagnosis.
ContributorsDuffy, Stacy Leigh (Author) / Lake, Douglas (Thesis advisor) / Magee, Dewey Mitch (Committee member) / Antwi, Kwasi (Committee member) / Arizona State University (Publisher)
Created2013
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Description
ABSTRACT

For almost a decade now, the Greek economic crisis has crippled the Greek nation and its citizenry. High unemployment rates as well as increased levels of homelessness and suicide are only some of the social repercussions of the collapse of the economic system. While we know much about the impact

ABSTRACT

For almost a decade now, the Greek economic crisis has crippled the Greek nation and its citizenry. High unemployment rates as well as increased levels of homelessness and suicide are only some of the social repercussions of the collapse of the economic system. While we know much about the impact of this crisis on Greek citizens, the literature surrounding the crisis lacks a full range of perspectives and experiences. This project works to fill-in the gaps surrounding the Greek economic crisis and the specific experiences of undocumented, immigrant, domestic workers. Looking at the ways in which these women exist in a constant state of violence, fear, and suffering I identify normalized violence in two main arenas: state/institutional and quotidian/everyday acts. Borrowing from Cecilia Menijvar’s pillars of normalized violence (2011), this work identifies the ways in which state-sponsored bureaucratic violence leads to real suffering and fear exemplified in moments of quotidian violence. Understanding the unique experiences of these women, works to weave together a more nuanced understanding of the impacts of the Greek economic crisis. Along with these moments of violence, this ethnographic inspired project highlights modes of survival, resistance, and resilience employed by these women in response to their violent circumstances.
ContributorsLinos, Viviane (Author) / Durfee, Alesha (Thesis advisor) / Vega, Sujey (Committee member) / Adelman, Madelaine (Committee member) / Arizona State University (Publisher)
Created2017