Matching Items (6)
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- All Subjects: Plant sciences
- All Subjects: carbon cycling
- Genre: Masters Thesis
- Creators: Throop, Heather
- Member of: Theses and Dissertations
Description
Dissolved organic matter (DOM) is an important part of aquatic foodwebs because it contains carbon, nitrogen, and other elements required by heterotrophic organisms. It has many sources that determine its molecular composition, nutrient content, and biological lability and in turn, influence whether it is retained and processed in the stream reach or exported downstream. I examined the composition of DOM from vascular wetland plants, filamentous algae, and riparian tree leaf litter in Sonoran Desert streams and its decomposition by stream microbes. I used a combination of field observations, in-situ experiments, and a manipulative laboratory incubation to test (1) how dominant primary producers influence DOM chemical composition and ecosystem metabolism at the reach scale and (2) how DOM composition and nitrogen (N) content control microbial decomposition and stream uptake of DOM. I found that differences in streamwater DOM composition between two distinct reaches of Sycamore Creek did not affect in-situ stream respiration and gross primary production rates. Stream sediment microbial respiration rates did not differ significantly when incubated in the laboratory with DOM from wetland plants, algae, and leaf litter, thus all sources were similarly labile. However, whole-stream uptake of DOM increased from leaf to algal to wetland plant leachate. Desert streams have the potential to process DOM from leaf, wetland, and algal sources, though algal and wetland DOM, due to their more labile composition, can be more readily retained and mineralized.
ContributorsKemmitt, Kathrine (Author) / Grimm, Nancy (Thesis advisor) / Hartnett, Hilairy (Committee member) / Throop, Heather (Committee member) / Arizona State University (Publisher)
Created2018
Description
Xylem conduits, a primary feature of most terrestrial plant taxa, deliver water to photosynthetic tissues and play a critical role in plant water relations and drought tolerance. Non-succulent woody taxa generally follow a universal rate of tip-to-base conduit widening such that hydraulic resistance remains constant throughout the plant stem. Giant cacti inhabit arid regions throughout the Americas and thrive in water-limited environments by complimenting water-storing succulent tissues with resource-efficient Crassulacean Acid Metabolism. Considering these adaptations, the objectives of this study were threefold: 1) determine whether xylem conduits in columnar cacti follow universal scaling theory as observed in woody taxa; 2) evaluate whether xylem hydraulic diameter is inversely correlated with xylem vessel density; and 3) determine whether xylem double-wall thickness-to-span ratio and other hydraulic architectural traits are convergent among phylogenetically diverse cactus species. This thesis investigates the xylem anatomy of nine cactus species native to the Sonoran Desert of Arizona and Mexico, the tropical dry forests of southern Mexico, and the Alto Plano region of Argentina. Soft xylem tissues closest to the stem apex underwent a modified polyethylene glycol treatment to stabilize for sectioning with a sledge microtome. Across all species: hydraulic diameter followed a basipetal widening rate of 0.21 (p < 0.001), closely matching the universal rate of 0.20 for woody taxa; and xylem vessel density was inversely correlated with both length from stem apex (p < 0.001) and hydraulic diameter (p < 0.001). Double-wall thickness-to-span ratio had little to no significant correlation with either length from stem apex or hydraulic diameter. There was no significant difference in hydraulic architectural trait patterns between phylogenetically diverse species with various stem morphologies, nor was there a significant correlation between conduit widening rates and volume-to-surface-area ratios.
This study demonstrates that giant cacti follow similar internal anatomical constraints as non-succulent woody taxa, yet stem succulence and water storage behavior in cacti remain separate from internal hydraulic architecture, allowing cacti to thrive in arid environments. Understanding how cacti cope with severe water limitations provides new insights on evolutionary constraints of stem succulents as they functionally diverged from other life forms.
ContributorsCaspeta, Ivanna (Author) / Hultine, Kevin (Thesis advisor) / Throop, Heather (Thesis advisor) / Hernandez, Tania (Committee member) / Arizona State University (Publisher)
Created2023
Description
The southwestern US will experience more frequent heat waves, prolonged droughts, and declining water supply. Riparian ecosystems are particularly at-risk under climate change predictions, but little is known about the thermal tolerance of plant species inhabiting these ecosystems. Populus fremontii, a pioneer and foundation tree species in riparian ecosystems throughout the southwest, is of concern given its importance in driving community structure and influencing ecosystem processes. This study compared leaf thermal tolerance across populations of P. fremontii to determine if local adaptation affects leaf thermal tolerance. I hypothesized that warm-adapted (low-elevation) populations would have larger leaf thermal tolerance thresholds, thermal safety margins, and thermal time constants than cool-adapted (high-elevation) populations. I expected warm-adapted populations to maintain lower maximum leaf temperatures due to local adaptation affecting leaf thermal regulation. Using a common garden at the warm edge of this species’ range, I measured leaf thermal tolerance metrics in eight populations spanning a 1,200 m elevational gradient. Data collection occurred in May, during mild air temperatures, and in August, during high air temperatures. The first two metrics were leaf thermal tolerance thresholds. The critical temperature (Tcrit) is the temperature at which the electron transport capacity of PSII is disrupted. T50 is the temperature at which the electron transport capacity decreases to 50%. The next metric was thermal safety margins (TSMs), which reflect a leaf’s vulnerability to reaching thermal tolerance thresholds. TSMs are the difference between either Tcrit or T50 and an experienced environmental variable such as leaf or air temperature. The last metric was the thermal time constant (?), which is a trait that represents how quickly leaf temperatures respond to changes in air temperatures. Tcrit, T50, and ? were not correlated with elevation regardless of season, suggesting that acclimation or phenotypic plasticity is affecting these metrics. Conversely, TSMs using maximum leaf temperature were negatively correlated with elevation in August because warm-adapted populations maintained lower maximum leaf temperatures. These findings suggest that warm-adapted populations are locally adapted to maintain cooler leaf temperatures, which is critical for their future survival since they do not maintain higher leaf thermal tolerance thresholds than cool-adapted populations.
ContributorsMoran, Madeline (Author) / Hultine, Kevin (Thesis advisor) / Throop, Heather (Thesis advisor) / Butterfield, Bradley (Committee member) / Arizona State University (Publisher)
Created2022
Description
Soil organic carbon (SOC) is a critical component of the global carbon (C) cycle, accounting for more C than the biotic and atmospheric pools combined. Microbes play an important role in soil C cycling, with abiotic conditions such as soil moisture and temperature governing microbial activity and subsequent soil C processes. Predictions for future climate include warmer temperatures and altered precipitation regimes, suggesting impacts on future soil C cycling. However, it is uncertain how soil microbial communities and subsequent soil organic carbon pools will respond to these changes, particularly in dryland ecosystems. A knowledge gap exists in soil microbial community responses to short- versus long-term precipitation alteration in dryland systems. Assessing soil C cycle processes and microbial community responses under current and altered precipitation patterns will aid in understanding how C pools and cycling might be altered by climate change. This study investigates how soil microbial communities are influenced by established climate regimes and extreme changes in short-term precipitation patterns across a 1000 m elevation gradient in northern Arizona, where precipitation increases with elevation. Precipitation was manipulated (50% addition and 50% exclusion of ambient rainfall) for two summer rainy seasons at five sites across the elevation gradient. In situ and ex situ soil CO2 flux, microbial biomass C, extracellular enzyme activity, and SOC were measured in precipitation treatments in all sites. Soil CO2 flux, microbial biomass C, extracellular enzyme activity, and SOC were highest at the three highest elevation sites compared to the two lowest elevation sites. Within sites, precipitation treatments did not change microbial biomass C, extracellular enzyme activity, and SOC. Soil CO2 flux was greater under precipitation addition treatments than exclusion treatments at both the highest elevation site and second lowest elevation site. Ex situ respiration differed among the precipitation treatments only at the lowest elevation site, where respiration was enhanced in the precipitation addition plots. These results suggest soil C cycling will respond to long-term changes in precipitation, but pools and fluxes of carbon will likely show site-specific sensitivities to short-term precipitation patterns that are also expected with climate change.
ContributorsMonus, Brittney (Author) / Throop, Heather L (Thesis advisor) / Ball, Becky A (Committee member) / Hultine, Kevin R (Committee member) / Munson, Seth M (Committee member) / Arizona State University (Publisher)
Created2019
Description
Climate change is increasing global surface temperatures, intensifying droughts and increasing rainfall variation, particularly in drylands. Understanding how dryland plant communities respond to climate change-induced rainfall changes is crucial for implementing effective conservation strategies. Concurrent with climate change impacts on drylands is woody encroachment: an increase in abundance of woody plant species in areas formerly dominated by grasslands or savannahs. For example, the woody plant, Prosopis velutina (velvet mesquite), has encroached into grasslands regionally over the past century. From an agricultural perspective, P. velutina is an invasive weed that hinders cattle forage. Understanding how P. velutina will respond to climate change-induced rainfall changes can be useful for management and conservation efforts. Prosopis velutina was used to answer the following question: Is there a significant interactive effect of mean soil water moisture content and pulse frequency on woody seedling survival and growth in dryland ecosystems? There were 256 P. velutina seedlings sourced from the Santa Rita Experimental Range in southern Arizona grown under four watering treatments where mean and pulse frequency were manipulated over two months. Data were collected on mortality, stem height, number of leaves, instantaneous gas exchange, chlorophyll fluorescence, biomass, and the leaf carbon to nitrogen (C:N) ratio. Mortality was low across treatments. Pulse frequency had less impact across response variables than the mean amount of water received. This may indicate that P. velutina seedlings are relatively insensitive to rainfall timing and are more responsive to rainfall amount. Prosopis velutina in the low mean soil moisture treatments lost a majority of their leaves and had greater biomass allocation to roots. Prosopis velutina’s ability to survive in low soil moisture conditions and invest in root biomass can allow it to persist as drylands are further affected by climate change. Prosopis velutina could benefit ecosystems where native plants are at risk due to rainfall variation if P. velutina occupies a similar niche space. Due to conflicting viewpoints of P. velutina as an invasive species, it’s important to examine P. velutina from both agricultural and conservation perspectives. Further analysis on the benefits to P. velutina in these ecosystems is recommended.
ContributorsDavis, Ashley R. (Author) / Throop, Heather (Thesis advisor) / Hultine, Kevin (Committee member) / Sala, Osvaldo (Committee member) / Arizona State University (Publisher)
Created2020
Description
Dioecious plants often display sexual segregation in habitat preference and trait expression due to contrasts in reproductive costs. Females may be maladapted to environments with limited available resources, or habitats where resources are diminishing due to climate change. Reduced fitness in female individuals compared to males could lead to skewed sex ratios and reduce population fitness of dioecious species, including one of the most widely distributed dioecious tree species in North America, Acer negundo. The goal of this study was to evaluate how climate warming and drought may enhance sexual segregation in productivity and physiological stress in A. negundo. To address this goal, I measured radial growth and carbon isotope ratios (δ13C) in tree-ring cellulose of 22-year male and female A. negundo trees growing in a common garden in Salt Lake City, UT. The trees were originally transplanted as one-year old cuttings from a nearby site that was 6.5 °C cooler that the common garden. I hypothesized that 1) δ13C would be lower (more negative) in late growth that is formed during the hottest months of the growing season in males than in females, and during years with no supplemental watering, indicating lower stress from heat and drought in males than in females. And 2) radial growth would be greater in males under warm, well-watered conditions and the addition of drought will exacerbate the difference between males and females. To test these hypotheses, cores were extracted from the main stem of nine male and nine female trees with an increment borer. Annual growth was measured on each core and cellulose was extracted to measure annual δ13C ratios. Males had a 0.63‰ lower mean δ13C than females in years after supplemental water had ceased (p = 0.03) and a 4.12 mm wider radial growth compared to females while irrigated (p = 0.02). Although these data did not support my hypotheses per se, results nevertheless indicate that females are more likely to be maladapted to climate warming and drought to a greater extent than males. If so, a combination of drought and heat stress may have deleterious impacts on the population fitness of Acer negundo and other similar dioecious tree species.
ContributorsChisholm, Mary (Author) / Hultine, Kevin R (Thesis advisor) / Throop, Heather (Thesis advisor) / Morino, Kiyomi (Committee member) / Arizona State University (Publisher)
Created2023