Matching Items (3)
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- All Subjects: Fertility
- All Subjects: Protein-protein interactions
- Creators: Bean, Heather
Description
The Multiple Antibiotic Resistance Regulator Family (MarR) are transcriptional regulators, many of which forms a dimer. Transcriptional regulation provides bacteria a stabilized responding system to ensure the bacteria is able to efficiently adapt to different environmental conditions. The main function of the MarR family is to create multiple antibiotic resistance from a mutated protein; this process occurs when the MarR regulates an operon. We hypothesized that different transcriptional regulator genes have interactions with each other. It is known that Salmonella pagC transcription is activated by three regulators, i.e., SlyA, MprA, and PhoP. Bacterial Adenylate Cyclase-based Two-Hybrid (BACTH) system was used to research the protein-protein interactions in SlyA, MprA, and PhoP as heterodimers and homodimers in vivo. Two fragments, T25 and T18, that lack endogenous adenylate cyclase activity, were used for construction of chimeric proteins and reconstruction of adenylate cyclase activity was tested. The significant adenylate cyclase activities has proved that SlyA is able to form homodimers. However, weak adenylate cyclase activities in this study has proved that MprA and PhoP are not likely to form homodimers, and no protein-protein interactions were detected in between SlyA, MprA and PhoP, which no heterodimers have formed in between three transcriptional regulators.
ContributorsTao, Zenan (Author) / Shi, Yixin (Thesis advisor) / Wang, Xuan (Committee member) / Bean, Heather (Committee member) / Arizona State University (Publisher)
Created2018
Description
One out of ten women has a difficult time getting or staying pregnant in the United States. Recent studies have identified aging as one of the key factors attributed to a decline in female reproductive health. Existing fertility diagnostic methods do not allow for the non-invasive monitoring of hormone levels across time. In recent years, olfactory sensing has emerged as a promising diagnostic tool for its potential for real-time, non-invasive monitoring. This technology has been proven promising in the areas of oncology, diabetes, and neurological disorders. Little work, however, has addressed the use of olfactory sensing with respect to female fertility. In this work, we perform a study on ten healthy female subjects to determine the volatile signature in biological samples across 28 days, correlating to fertility hormones. Volatile organic compounds (VOCs) present in the air above the biological sample, or headspace, were collected by solid phase microextraction (SPME), using a 50/30 µm divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) coated fiber. Samples were analyzed, using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS). A regression model was used to identify key analytes, corresponding to the fertility hormones estrogen and progesterone. Results indicate shifts in volatile signatures in biological samples across the 28 days, relevant to hormonal changes. Further work includes evaluating metabolic changes in volatile hormone expression as an early indicator of declining fertility, so women may one day be able to monitor their reproductive health in real-time as they age.
ContributorsOng, Stephanie (Author) / Smith, Barbara (Thesis advisor) / Bean, Heather (Committee member) / Plaisier, Christopher (Committee member) / Arizona State University (Publisher)
Created2018
Description
This dissertation explores changes in fertility and family formation in South Korea, a setting in which rapid demographic changes have taken place since the early twentieth century. Despite active debate and discussion among experts and policymakers, knowledge is still limited in regards to the country’s significant demographic changes. I take advantage of Korean census samples data from 1966 to 2010, which span birth cohorts from pre- and early-transitional stages to post-transitional stages, which comprise the entry stage of the second demographic transition. From a cohort perspective, I use diverse demographic methods to analyze three different aspects of fertility and family formation—fertility differentials, marriage delay, and fertility concentration.
The findings illustrate how fertility and marriage patterns have changed over generations and range from a politically tumultuous period, which includes World War II, liberation, and the Korean War, to an advanced economic period. By and large, the three studies suggest that until 1960, fertility and family formation converged as per social norms and leadership guidelines. Then, marriage and childbearing behaviors began to diversify and variation by social groups increased for cohorts born during and after the 1960s. The phrase “convergence towards diversity” captures the reversal of demographic trends within the country. Taken together, this dissertation advances our understanding of how fertility and family formation have changed in South Korea, which has been on an intense demographic journey from pre-transitional fertility through very low fertility, and currently headed toward another destination.
The findings illustrate how fertility and marriage patterns have changed over generations and range from a politically tumultuous period, which includes World War II, liberation, and the Korean War, to an advanced economic period. By and large, the three studies suggest that until 1960, fertility and family formation converged as per social norms and leadership guidelines. Then, marriage and childbearing behaviors began to diversify and variation by social groups increased for cohorts born during and after the 1960s. The phrase “convergence towards diversity” captures the reversal of demographic trends within the country. Taken together, this dissertation advances our understanding of how fertility and family formation have changed in South Korea, which has been on an intense demographic journey from pre-transitional fertility through very low fertility, and currently headed toward another destination.
ContributorsYoo, Sam Hyun (Author) / Sarah, Hayford (Thesis advisor) / Agadjanian, Victor (Committee member) / Yabiku, Scott (Committee member) / Arizona State University (Publisher)
Created2015