Matching Items (3)
Filtering by
- All Subjects: Plasma
- Creators: Boehringer, Brian Thomas
- Creators: Lake, Douglas
- Creators: Shovkovy, Igor
- Status: Published
Description
Extracellular vesicles (EVs) represent a heterogeneous population of small vesicles, consisting of a phospholipidic bilayer surrounding a soluble interior cargo. These vesicles play an important role in cellular communication by virtue of their protein, RNA, and lipid content, which can be transferred among cells. Peripheral blood is a rich source of circulating EVs. An analysis of EVs in peripheral blood could provide access to unparalleled amounts of biomarkers of great diagnostic, prognostic as well as therapeutic value. In the current study, a plasma EV enrichment method based on pluronic co-polymer was first established and characterized. Plasma EVs from breast cancer patients were then enriched, profiled and compared to non-cancer controls. Proteins signatures that contributed to the prediction of cancer samples from non-cancer controls were created by a random-forest based cross-validation approach. We found that a large portion of these signatures were related to breast cancer aggression. To verify such findings, KIAA0100, one of the features identified, was chosen for in vitro molecular and cellular studies in the breast cancer cell line MDA-MB-231. We found that KIAA0100 regulates cancer cell aggression in MDA-MB-231 in an anchorage-independent manner and is particularly associated with anoikis resistance through its interaction with HSPA1A. Lastly, plasma EVs contain not only individual proteins, but also numerous molecular complexes. In order to measure millions of proteins, isoforms, and complexes simultaneously, Adaptive Dynamic Artificial Poly-ligand Targeting (ADAPT) platform was applied. ADAPT employs an enriched library of single-stranded oligodeoxynucleotides to profile complex biological samples, thus achieving a deep coverage of system-wide, native biomolecules. Profiling of EVs from breast cancer patients was able to obtain a prediction AUC performance of 0.73 when compared biopsy-positive cancer patient to healthy controls and 0.64 compared to biopsy-negative controls and such performance was not associated with the physical breast condition indicated by BIRAD scores. Taken together, current research demonstrated the potential of profiling plasma EVs in searching for therapeutic targets as well as diagnostic signatures.
ContributorsZhong, Zhenyu (Author) / Spetzler, David (Thesis advisor) / Yan, Hao (Thesis advisor) / Lake, Douglas (Committee member) / Mangone, Marco (Committee member) / Arizona State University (Publisher)
Created2018
Description
Chiral symmetry and its anomalous and spontaneous breaking play an important role
in particle physics, where it explains the origin of pion and hadron mass hierarchy
among other things.
Despite its microscopic origin chirality may also lead to observable effects
in macroscopic physical systems -- relativistic plasmas made of chiral
(spin-$\frac{1}{2}$) particles.
Such plasmas are called \textit{chiral}.
The effects include non-dissipative currents in external fields that could be present
even in quasi-equilibrium, such as the chiral magnetic (CME) and separation (CSE)
effects, as well as a number of inherently chiral collective modes
called the chiral magnetic (CMW) and vortical (CVW) waves.
Applications of chiral plasmas are truly interdisciplinary, ranging from
hot plasma filling the early Universe, to dense matter in neutron stars,
to electronic band structures in Dirac and Weyl semimetals, to quark-gluon plasma
produced in heavy-ion collisions.
The main focus of this dissertation is a search for traces of chiral physics
in the spectrum of collective modes in chiral plasmas.
I start from relativistic chiral kinetic theory and derive
first- and second-order chiral hydrodynamics.
Then I establish key features of an equilibrium state that describes many
physical chiral systems and use it to find the full spectrum of collective modes
in high-temperature and high-density cases.
Finally, I consider in detail the fate of the two inherently chiral waves, namely
the CMW and the CVW, and determine their detection prospects.
The main results of this dissertation are the formulation of a fully covariant
dissipative chiral hydrodynamics and the calculation of the spectrum of collective
modes in chiral plasmas.
It is found that the dissipative effects and dynamical electromagnetism play
an important role in most cases.
In particular, it is found that both the CMW and the CVW are heavily damped by the usual
Ohmic dissipation in charged plasmas and the diffusion effects in neutral plasmas.
These findings prompt a search for new physical observables in heavy-ion collisions,
as well as a revision of potential applications of chiral theories in
cosmology and solid-state physics.
in particle physics, where it explains the origin of pion and hadron mass hierarchy
among other things.
Despite its microscopic origin chirality may also lead to observable effects
in macroscopic physical systems -- relativistic plasmas made of chiral
(spin-$\frac{1}{2}$) particles.
Such plasmas are called \textit{chiral}.
The effects include non-dissipative currents in external fields that could be present
even in quasi-equilibrium, such as the chiral magnetic (CME) and separation (CSE)
effects, as well as a number of inherently chiral collective modes
called the chiral magnetic (CMW) and vortical (CVW) waves.
Applications of chiral plasmas are truly interdisciplinary, ranging from
hot plasma filling the early Universe, to dense matter in neutron stars,
to electronic band structures in Dirac and Weyl semimetals, to quark-gluon plasma
produced in heavy-ion collisions.
The main focus of this dissertation is a search for traces of chiral physics
in the spectrum of collective modes in chiral plasmas.
I start from relativistic chiral kinetic theory and derive
first- and second-order chiral hydrodynamics.
Then I establish key features of an equilibrium state that describes many
physical chiral systems and use it to find the full spectrum of collective modes
in high-temperature and high-density cases.
Finally, I consider in detail the fate of the two inherently chiral waves, namely
the CMW and the CVW, and determine their detection prospects.
The main results of this dissertation are the formulation of a fully covariant
dissipative chiral hydrodynamics and the calculation of the spectrum of collective
modes in chiral plasmas.
It is found that the dissipative effects and dynamical electromagnetism play
an important role in most cases.
In particular, it is found that both the CMW and the CVW are heavily damped by the usual
Ohmic dissipation in charged plasmas and the diffusion effects in neutral plasmas.
These findings prompt a search for new physical observables in heavy-ion collisions,
as well as a revision of potential applications of chiral theories in
cosmology and solid-state physics.
ContributorsRybalka, Denys (Author) / Shovkovy, Igor (Thesis advisor) / Lunardini, Cecilia (Committee member) / Timmes, Francis (Committee member) / Vachaspati, Tanmay (Committee member) / Arizona State University (Publisher)
Created2019
Description
A high voltage plasma arc can be created and sustained in air by subjecting the gases to an electric field with high voltage potential, causing ionization. The internal energy of the ionized gases can be transferred to corresponding pressure waves when the matter involved switches between the gaseous and plasma states. By pulse-width modulating a transformer driving signal, the transfer of internal electrical energy to resonating pressure waves may be controlled. Audio wave input to the driver signal can then be modulated into the carrier wave and be used to determine the width of each pulse in the plasma, thus reconstructing the audio signal as pressure, or sound waves, as the plasma arc switches on and off. The result will be the audio waveform resonating out of the plasma arc as audible sound, and thus creating a plasma loudspeaker. Theory of operation was tested through construction of a plasma arc speaker, and resultant audio playback was analyzed. This analysis confirmed accurate reproduction of audio signal in audible sound.
ContributorsBoehringer, Brian Thomas (Author) / Roedel, Ronald (Thesis director) / Huffman, James (Committee member) / Barrett, The Honors College (Contributor) / Electrical Engineering Program (Contributor)
Created2014-05