Matching Items (2)
Filtering by

Clear all filters

136133-Thumbnail Image.png
Description
Currently in synthetic biology only the Las, Lux, and Rhl quorum sensing pathways have been adapted for broad engineering use. Quorum sensing allows a means of cell to cell communication in which a designated sender cell produces quorum sensing molecules that modify gene expression of a designated receiver cell. While

Currently in synthetic biology only the Las, Lux, and Rhl quorum sensing pathways have been adapted for broad engineering use. Quorum sensing allows a means of cell to cell communication in which a designated sender cell produces quorum sensing molecules that modify gene expression of a designated receiver cell. While useful, these three quorum sensing pathways exhibit a nontrivial level of crosstalk, hindering robust engineering and leading to unexpected effects in a given design. To address the lack of orthogonality among these three quorum sensing pathways, previous scientists have attempted to perform directed evolution on components of the quorum sensing pathway. While a powerful tool, directed evolution is limited by the subspace that is defined by the protein. For this reason, we take an evolutionary biology approach to identify new orthogonal quorum sensing networks and test these networks for cross-talk with currently-used networks. By charting characteristics of acyl homoserine lactone (AHL) molecules used across quorum sensing pathways in nature, we have identified favorable candidate pathways likely to display orthogonality. These include Aub, Bja, Bra, Cer, Esa, Las, Lux, Rhl, Rpa, and Sin, which we have begun constructing and testing. Our synthetic circuits express GFP in response to a quorum sensing molecule, allowing quantitative measurement of orthogonality between pairs. By determining orthogonal quorum sensing pairs, we hope to identify and adapt novel quorum sensing pathways for robust use in higher-order genetic circuits.
ContributorsMuller, Ryan (Author) / Haynes, Karmella (Thesis director) / Wang, Xiao (Committee member) / Barrett, The Honors College (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / Department of Chemistry and Biochemistry (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description

By studying the workflow used to create the black hole, Gargantua, in Interstellar, artists can understand how to simulate complex astronomical phenomena in other special effects software such as Houdini. This workflow utilizes a balance between scientific realism and artistic interpretation of astronomical phenomena such that simulations can maximize their

By studying the workflow used to create the black hole, Gargantua, in Interstellar, artists can understand how to simulate complex astronomical phenomena in other special effects software such as Houdini. This workflow utilizes a balance between scientific realism and artistic interpretation of astronomical phenomena such that simulations can maximize their success in film. Through significant amounts of research and study, the artists at Double Negative generated a scientifically realistic black hole in shape and physical behavior, but made creative decisions when shading and lighting their simulation. I find that DNeg’s workflow integrates well when using Houdini technology. I follow their workflow to generate a series of spiral galaxies in Houdini and find how Houdini’s node network layout allows artists to incorporate both scientific realism and creative approaches to a simulation. A strong understanding of the mechanics of the simulated astronomical event scientifically informs the look and shape of a production, while Houdini’s node network layout makes it easy for special effects artists to manipulate simulations to their own artistic interpretation of astronomical phenomena.

ContributorsJoiner, Jae (Author) / Kim, Sujin (Thesis director) / Lawson, Shawn (Committee member) / Barrett, The Honors College (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / School of Art (Contributor)
Created2023-05