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- All Subjects: Honeybees
- All Subjects: Ecdysone
- Creators: Ozturk, Cahit
- Creators: Cook, Chelsea
Description
Honeybees (Apis mellifera) are pollinators that face multiple challenges during foraging such as fungicides applied to floral sources. Fungicides are chemicals used to inhibit key fungal mechanisms like metabolism, but their effects remain relatively unknown in bees. In addition, studying the maturing bee can help us identify demographics that are more vulnerable to toxic materials like fungicides. The purpose of this study is test whether maturation and the fungicide Pristine influence the permeability of the blood-brain barrier. Specifically, we use a transportable dye to test how blood brain barrier transporter function responds to toxic insult and how it changes with age. Oral ingestion of Pristine by female workers did not have an effect on blood brain barrier permeability which suggests Pristine may have no or longer term consequences in the bee. However, blood brain barrier permeability changed with the bee's age which could be explained by the regulation of blood brain barrier transporters during natural transitions in hive task or the presence of hemolymph protein filtration
ContributorsPatel, Aamir S. (Author) / Amdam, Gro (Thesis director) / Harrison, Jon (Committee member) / Ozturk, Cahit (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Division of labor is a hallmark for social insects and is closely related to honey bee morphology and physiology. Vitellogenin (Vg), a precursor protein in insect egg yolk, has several known functions apart from serving as a nutrient source for developing eggs. Vg is a component in the royal jelly produced in the hypopharyngeal glands (HPG) of worker bees which is used to feed both the developing brood and the queen. The HPG is closely associated with divisions of labor as the peak in its development corresponds with the nursing behavior. Independent of the connection between Vg and the HPG, Vg has been seen to play a fundamental role in divisions of labor by affecting worker gustatory responses, age of onset of foraging, and foraging preferences. Similar to Vg, the number of ovarioles in worker ovaries is also associated with division of labor as bees with more ovarioles tend to finish tasks in the hive and become foragers faster. This experiment aims to connect HPGs, ovaries, and Vg by proposing a link between them in the form of ecdysone (20E). 20E is a hormone produced by the ovaries and is linked to ovary development and Vg by tyramine titers. By treating young emerged bees with ecdysone and measuring HPG and ovary development over a trial period, this experiment seeks to determine whether 20E affects division of labor through Vg. We found that though the stress of injection caused a significant decrease in development of both the ovaries and HPG, there was no discernable effect of 20E on either of these organs.
ContributorsChin, Elijah Seth (Author) / Wang, Ying (Thesis director) / Page, Robert (Committee member) / Cook, Chelsea (Committee member) / School of Molecular Sciences (Contributor) / W. P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2016-12
Description
Social insects collectively exploit food sources by recruiting nestmates, creating positive feedback that steers foraging effort to the best locations. The nature of this positive feedback varies among species, with implications for collective foraging. The mass recruitment trails of many ants are nonlinear, meaning that small increases in recruitment effort yield disproportionately large increases in recruitment success. The waggle dance of honeybees, in contrast, is believed to be linear, meaning that success increases proportionately to effort. However, the implications of this presumed linearityhave never been tested. One such implication is the prediction that linear recruiters will equally exploit two identical food sources, in contrast to nonlinear recruiters, who randomly choose only one of them. I tested this prediction in colonies of honeybees that were isolated in flight cages and presented with two identical sucrose feeders. The results from 15 trials were consistent with linearity, with many cases of equal exploitation of the feeders. In addition, I tested the prediction that linear recruiters can reallocate their forager distribution when unequal feeders are swapped in position. Results from 15 trials were consistent with linearity, with many cases of clear choice for a stronger food source, followed by a subsequent switch with reallocation of foragers to the new location of the stronger food source. These findings show evidence of a linear pattern of nestmate recruitment, with implications for how colonies effectively distribute their foragers across
available resources.
ContributorsAlam, Showmik (Author) / Shaffer, Zachary (Thesis advisor) / Pratt, Stephen C (Thesis advisor) / Ozturk, Cahit (Committee member) / Pavlic, Theodore (Committee member) / Arizona State University (Publisher)
Created2022