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ABSTRACT Enzyme-Induced Carbonate Precipitation (EICP) using a plant-derived form of the urease enzyme to induce the precipitation of calcium carbonate (CaCO3) shows promise as a method of stabilizing soil for the mitigation of fugitive dust. Fugitive dust is a significant problem in Arizona, particularly in Maricopa County. Maricopa County is

ABSTRACT Enzyme-Induced Carbonate Precipitation (EICP) using a plant-derived form of the urease enzyme to induce the precipitation of calcium carbonate (CaCO3) shows promise as a method of stabilizing soil for the mitigation of fugitive dust. Fugitive dust is a significant problem in Arizona, particularly in Maricopa County. Maricopa County is an EPA air quality non-attainment zone, due primarily to fugitive dust, which presents a significant health risk to local residents. Conventional methods for fugitive dust control, including the application of water, are either ineffective in arid climates, very expensive, or limited to short term stabilization. Due to these limitations, engineers are searching for new and more effective ways to stabilize the soil and reduce wind erosion. EICP employs urea hydrolysis, a process in which carbonate precipitation is catalyzed by the urease enzyme, a widely occurring protein found in many plants and microorganisms. Wind tunnel experiments were conducted in the ASU/NASA Planetary Wind Tunnel to evaluate the use of EICP as a means to stabilize soil against fugitive dust emission. Three different soils were tested, including a native Arizona silty-sand, a uniform fine to medium grained silica sand, and mine tailings from a mine in southern Arizona. The test soil was loosely placed in specimen container and the surface was sprayed with an aqueous solution containing urea, calcium chloride, and urease enzyme. After a short period of time to allow for CaCO3 precipitation, the specimens were tested in the wind tunnel. The completed tests show that EICP can increase the detachment velocity compared to bare or wetted soil and thus holds promise as a means of mitigating fugitive dust emissions.
ContributorsKnorr, Brian (Author) / Kavazanjian, Edward (Thesis advisor) / Houston, Sandra (Committee member) / Zapata, Claudia (Committee member) / Arizona State University (Publisher)
Created2014
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Description
Enzyme-induced carbonate precipitation (EICP) is an emerging technology for ground improvement that cements soil with calcium carbonate to increase strength and stiffness. EICP-improved soil can be used to support new facilities or it can be injected under existing facilities to prevent excessive deformation. The limitations for commercial adoption of EICP

Enzyme-induced carbonate precipitation (EICP) is an emerging technology for ground improvement that cements soil with calcium carbonate to increase strength and stiffness. EICP-improved soil can be used to support new facilities or it can be injected under existing facilities to prevent excessive deformation. The limitations for commercial adoption of EICP are the cost and the lack of implementation at field-scale. This research demonstrated two ways to reduce the cost of EICP treatment at field-scale. The first was a modification to the EICP solution such that lower amounts of chemicals are needed to achieve target strengths. The second was to use a simple and inexpensive enzyme extraction method to produce the enzyme at a large-scale. This research also involved a two-stage scale-up process to create EICP biocemented soil columns using a permeation grouting technique. The first stage was at mid-scale where 0.6 m x 0.3 m-diameter EICP biocemented soil columns were created in boxes. This work confirmed that conventional permeation grouting equipment and methods are feasible for EICP soil treatment because the columns were found to have a uniform shape, the injection method was able to deliver the EICP solution to the edges of the treatment zone, and downhole geophysics was effectively used to measure the shear wave velocity of the biocemented soil mass. The field-scale stage was performed in the Test Pit facility at the Center for Bio-mediated and Bio-inspired Geotechnics' Soils Field Laboratory. Seven biocemented soil columns were created with diameters ranging from 0.3-1 m and heights ranging from 1-2.4 m. Effective implementation at this scale was confirmed through monitoring the injection process with embedded moisture sensors, evaluating the in situ strength improvement with downhole geophysics and load testing, and testing of the excavated columns to measure shear wave velocity, dimensions, carbonate content, and strength. Lastly, a hotspot life cycle assessment was performed which identified ways to reduce the environmental impacts of EICP by using alternative sourcing of inputs and extraction of byproducts. Overall, this research project demonstrates that EICP is a viable ground improvement technique by way of successfully producing field-scale biocemented soil columns.
ContributorsMartin, Kimberly Kathryn (Author) / Kavazanjian, Jr., Edward (Thesis advisor) / Zapata, Claudia E. (Committee member) / van Paassen, Leon (Committee member) / Arizona State University (Publisher)
Created2021
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Description
Enzyme induced carbonate precipitation (EICP) treatment is a stabilization method of dust mitigation that applies a spray-on treatment to form a soil crust and increase the wind erosion resistance of a disturbed soil surface. The purpose of this work was to evaluate the EICP treatment with multiple field and laboratory

Enzyme induced carbonate precipitation (EICP) treatment is a stabilization method of dust mitigation that applies a spray-on treatment to form a soil crust and increase the wind erosion resistance of a disturbed soil surface. The purpose of this work was to evaluate the EICP treatment with multiple field and laboratory test methods for measuring the wind erosion resistance of EICP treated soil. The threshold friction velocity (TFV) is defined as the minimum wind speed required to initiate continuous particle movement and represents the wind erosion resistance of a soil surface. Tested soil type and textures included a clean fine sand to a loamy sandy soil that contained a significant amount of fines. Dry untreated soil and disturbed field soil surfaces were compared to a chloride salt solution treatment and an EICP treatment solution in both laboratory and field testing to evaluate the wind erosion resistance of the treatments.
ContributorsWoolley, Miriam Arna (Author) / Kavazajian, Edward (Thesis advisor) / van Paassen, Leon (Committee member) / Khodadaditirkolaei, Hamed (Committee member) / Hamdan, Nasser (Committee member) / Arizona State University (Publisher)
Created2023