Matching Items (2)
Filtering by
- Creators: School of Life Sciences
Description
Alternative polyadenylation (APA) is the biological mechanism in which the same gene can have multiple 3'untranslated region (3'UTR) isoforms due to the presence of multiple polyadenylation signal (PAS) elements within the pre mRNAs. Because APA produces mRNA transcripts that have different 3'UTR isoforms, certain transcripts may be subject to post-transcriptional regulation by regulatory non-coding RNAs, such as microRNAs or RNA binding proteins defects of which have been implicated in diseases such as cancer. Despite the increasing level of information, functional understanding of the molecular mechanisms involved in transcription is still poorly understood, nor is it clear why APA is necessary at a cell or tissue-specific level. To address these questions I wanted to develop a set of sensor strain plasmids capable of detecting cleavage and polyadenylation in vivo, inject the complete sensor strain plasmid into C. elegans and prepare stable transgenic lines, and perform proof-of-principle RNAi feeding experiments targeting genes associated with the cleavage and polyadenylation complex machinery. I demonstrated that it was possible to create a plasmid capable of detecting cleavage and polyadenylation in C. elegans; however, issues arose during the RNAi assays indicating the sensor strain plasmid was not sensitive enough to the RNAi to effectively detect in the worms. Once the problems involved with sensitivity and variability in the RNAi effects are resolved, the plasmid would be able to better address questions regarding the functional understanding of molecular mechanisms involved in transcription termination.
ContributorsWilky, Henry Patrick (Author) / Mangone, Marco (Thesis director) / Newbern, Jason (Committee member) / Blazie, Stephen (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
The bulk of this thesis is the translation itself—of Episodes 8-10—with timed subtitles. However, I also wanted to provide commentary on a few issues of interest that I came across during the translation. Conveying meaning from one language to the other does not simply involve translating the denotations of each word, but also the social context, humor, tone, and other aspects surrounding the situation. Whether words are used individually to interject, or come together to form unique idioms or phrases, the challenge presented to the translator is the choices: what words do I select to best convey not only the dictionary sense, but also the contextual meaning? This is especially difficult in wordplay-type situations. If there’s no perfect option, can I find a cultural analog that differs from the original denotation, but preserves the intention of the words? Otherwise, do I find the original important enough that it is worth educating the reader in order to preserve it—or, do I see no other translation option but to educate as a last resort? These are all choices that I had to make, and in this thesis I will be discussing the most interesting situations that came up, and my choices in dealing with them.
ContributorsLiong, Katerina Vakana (Author) / Tueller, Michael A. (Thesis director) / Printezis, Antonios (Committee member) / School of Life Sciences (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05