The study of macaque monkeys harbors advancements in the field of biomedical research. It is imperative to understand the genetic composition of different species of macaques to assess their accuracy as non-human primate (NHP) models for disease detection and treatment assessments. We sought to characterize the hybridization and admixture of the Southeast Asian macaques using single nucleotide polymorphism markers and analyzing the populations on the mainland and the island. Using AMOVA tests and STRUCTURE analysis, we determined that there are three distinct populations: Macaca mulatta, M. fascicularis fascicularis, and M. f. aurea. Furthermore, the island species holds an isolated population of M. f. aurea that demonstrate high inbreeding and genetic uniqueness compared to the mainland species. Findings from this study confirm that NHP models may need to be modified or updated according to changing allelic frequencies and genetic drift.

Cancers of the reproductive tissues make up a significant portion of the cancer burden and mortality experienced by humans. Humans experience several proximal causative carcinogens that explain a portion of cancer risk, but an evolutionary viewpoint can provide a unique lens into the ultimate causes of reproductive cancer vulnerabilities. A life history framework allows us to make predictions on cancer prevalence based on a species’ tempo of reproduction. Moreover, certain variations in the susceptibility and prevalence of cancer may emerge due to evolutionary trade-offs between reproduction and somatic maintenance. For example, such trade-offs could involve the demand for rapid proliferation of cells in reproductive tissues that arises with reproductive events. In this study, I compiled reproductive cancer prevalence for 158 mammalian species and modeled the predictive power of 13 life history traits on the patterns of cancer prevalence we observed, such as Peto’s Paradox or slow-fast life history strategies. We predicted that fast-life history strategists will exhibit higher neoplasia prevalence risk due to reproductive trade-offs. Furthering this analytical framework can aid in predicting cancer rates and stratifying cancer risk across the tree of life.

Cells have mechanisms in place to maintain the specific lipid composition of distinct organelles including vesicular transport by the endomembrane system and non-vesicular lipid transport by lipid transport proteins. Oxysterol Binding Proteins (OSBPs) are a family of lipid transport proteins that transfer lipids at various membrane contact sites (MCSs). OSBPs have been extensively investigated in human and yeast cells where twelve have been identified in Homo sapiens and seven in Saccharomyces cerevisiae. The evolutionary relationship between these well-characterized OSBPs is still unclear. Reconstructed OSBP phylogenies revealed that the ancestral Saccharomycotinan had four OSBPs, the ancestral Holomycotan had five OSBPs, the ancestral Holozoan had six OSBPs, the ancestral Opisthokont had three OSBPs, and the ancestral Eukaroyte had three OSBPs. Our analysis identified three clades of ancient OSBPs not present in animals or fungi.

Pathogenic drug resistance is a major global health concern. Thus, there is great interest in modeling the behavior of resistant mutations–how quickly they will rise in frequency within a population, and whether they come with fitness tradeoffs that can form the basis of treatment strategies. These models often depend on precise measurements of the relative fitness advantage (s) for each mutation and the strength of the fitness tradeoff that each mutation suffers in other contexts. Precisely quantifying s helps us create better, more accurate models of how mutants act in different treatment strategies. For example, P. falciparum acquires antimalarial drug resistance through a series of mutations to a single gene. Prior work in yeast expressing this P. falciparum gene demonstrated that mutations come with tradeoffs. Computational work has demonstrated the possibility of a treatment strategy which enriches for a particular resistant mutation that then makes the population grow poorly once the drug is removed. This treatment strategy requires knowledge of s and how it changes when multiple mutants are competing across various drug concentrations. Here, we precisely quantified s in varying drug concentrations for five resistant mutants, each of which provide varying degrees of drug resistance to antimalarial drugs. DNA barcodes were used to label each strain, allowing the mutants to be pooled together for direct competition in different concentrations of drug. This will provide data that can make the models more accurate, potentially facilitating more effective drug treatments in the future.

Theories about the human origin in evolution and religion are fundamentally countering beliefs that are still debated to this day. This study continues to explore this relationship in the college population at a public university with the intention of targeting a diverse religious population. This research hopes to answer the question: does having greater literacy in evolution lead to a noninterventionist perspective on evolution? The prediction is that evidence of increased evolution comprehension will influence students to have a more agnostic, or noninterventionist, view on evolution. An evolution class was given a survey that had two parts broken into demographic and evolution sections with one question that asks about compatibility between evolution and religion. This was given twice in a single semester to track the growth of evolution knowledge and any other differences. There were 265 students in the initial survey, but only 223 responses in the post-survey. The compatibility question had 8 statements that range from creationist to atheistic perspectives and was divided into two sides: interventionist (divine involvement) and noninterventionist (deity may be present but does not intervene). More than 70% of the class had a noninterventionist perspective on evolution despite the Christian categories being the second largest group students identified with after agnostic. The agnostic statement was the top choice followed by the atheistic answer on the noninterventionist side. Lastly, there was some growth of evolution knowledge for each religious category in the evolution section but is not significant for interpretation. Based on the collected data, it is not sufficient to answer the question and requires more data collection via a longitudinal study.

Discussions of the nature of time can be enriched using insight from the field of biology. In the evolution of our species, biological timekeeping mechanisms have been honed to a fine point and can accurately adhere to a 24-hour cycle. When considering that these timekeeping mechanisms would only have evolved if they were reasonably accurate, it must be the case that our timekeeping systems are measuring a genuine feature of reality. This thesis explores this idea by discussing the veridicality of the circadian clock and contrasting it with the physiological origin of feelings of duration. As it turns out, duration can not be accounted for by the circadian clock, implying that the felt ‘flow’ of time is a cognitive illusion rather than a feature of objective reality. Given this, our knowledge of biology reconciles best with the Static View (B-theory) of time.