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- Creators: School of Life Sciences
- Creators: Department of Psychology
Does assisted cycle therapy influence activities of daily living in older adults with Down syndrome?
Alzheimer’s disease is a disease that can affect cognition, perception and behavior and is currently untreatable. It was discovered in the early 20th century and while significant scientific advancements have occurred, there is ambiguity that remains to be researched and understood. Latinos are the largest ethnic minority in the United States and while data still needs to be uncovered, possible risk factors for developing Alzheimer’s include heart issues, poverty and obesity, age and education level, to name a few. Poverty is linked to obesity, diabetes and a low education level, which in turn have been found to have an impact on Alzheimer’s and all factors impact cardiovascular and vascular health. Due to the collectivistic culture that is deeply rooted in Latinos, there is a strong sense of family that is upheld when caring for relatives who are afflicted and may be hesitant to receive the care that is needed. Other barriers include financial stability, linguistic and cultural barriers, underutilizing resources and health literacy. There are still research gaps that are yet to be filled like brain health and longitudinal studies for Latinos, but current treatments like diet and culturally competent professionals can help with the prognosis. Alzheimer’s is a complex disease, but with the numerous efforts made thus far, such as creating the LatinosAgainstAlzheimer’s Network, it will soon be able to be understood and hopefully eradicated.
understanding of the disease pathology, more efficacious drug development and
regenerative medicine as a form of treatment. There are limitations with current
transgenic mouse models of Alzheimer’s disease and the study of post mortem brain tissue of Alzheimer’s diseases patients. Stem cell models can overcome the lack of clinical relevance and impracticality associated with current models. Ideally, the use of stem cell models provides the foundation to study the biochemical and physiological aspects of Alzheimer’s disease, but at the cellular level. Moreover, the future of drug development and disease modeling can be improved by developing a reproducible and well-characterized model of AD that can be scaled up to meet requirements for basic and translational applications. Characterization and analysis of a heterogenic neuronal culture developed from induced pluripotent stem cells calls for the understanding of single cell identity and cell viability. A method to analyze RNA following intracellular sorting was developed in order to analyze single cell identity of a heterogenic population
of human induced pluripotent stem cells and neural progenitor cells. The population was intracellularly stained and sorted for Oct4. RNA was isolated and analyzed with qPCR, which demonstrated expected expression profiles for Oct4+ and Oct4- cells. In addition, a protocol to label cells with pO2 sensing nanoprobes was developed to assess cell viability. Non-destructive nanoprobe up-take by neural progenitor cells was assessed with fluorescent imaging and flow cytometry. Nanoprobe labeled neurons were cultured long-term and continued to fluoresce at day 28. The proof of concept experiments demonstrated will be further expanded upon and utilized in developing a more clinically relevant and cost-effective model of Alzheimer’s disease with downstream applications
in drug development and regenerative medicine.
Overview: There has been very little research done into the topic of mental illness in general, and Alzheimer’s Disease specifically, in Guatemala. The existing research accounts for prevalence of mental illness in Guatemala with an estimated prevalence of a mental illness of 27.8% (Guatemalan Government, 2009). Alzheimer’s Disease is less well researched.
Research Question: This research addresses this gap in knowledge by focusing on the stigma felt toward people who had Alzheimer’s Disease and Related Dementia (ADRD) by the people of Guatemala.
Participants: One-hundred twenty-four individuals over the age of 18 were recruited for participation. Participants were recruited through opportunity samples in artisan markets in Antigua.
Procedures: Participants completed a survey including demographic questions, the Dementia Attitudes Scale (O’Connor & McFadden 2010), as well as open-ended questions regarding the causes, symptoms, and treatments for Alzheimer’s. The study was conducted from July 2, 2018 to August 2, 2018.
Results: The average DAS score of 100.31± 14.01 found in this study is similar to results from other studies conducted in the United States (O'Connor & McFadden, 2010). Factor analysis did not verify the existence of sub-scales in the survey, as found in previous studies. The free-response questions indicated that many people may believe that ADRD is an inherited disease or one that is caused by factors outside of their control.
Conclusions: The high DAS score of 100.31± 14.01 matches other studies that used the DAS. Scores of 103.51± 13.43 (Scerri & Scerri, 2013) were reported in other studies and interpreted as positive as it relates to stigma. This points to a low stigma level in Guatemala. The failure to verify the sub-scales leads to the conclusion that although scales are validated in western nations, they may not be culturally portable. The DAS scale may not be measuring the same thing in this sample’s population versus previous studies sample populations.