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- All Subjects: Identification
- Creators: Castillo Gutiérrez, Josemar Andreina
- Creators: Chastain, Hope Natasha
- Creators: Fox, Cora
Description
Bioparticles comprise a diverse amount of materials ubiquitously present in nature. From proteins to aerosolized biological debris, bioparticles have important roles spanning from regulating cellular functions to possibly influencing global climate. Understanding their structures, functions, and properties provides the necessary tools to expand our fundamental knowledge of biological systems and exploit them for useful applications. In order to contribute to this efforts, the work presented in this dissertation focuses on the study of electrokinetic properties of liposomes and novel applications of bioaerosol analysis. Using immobilized lipid vesicles under the influence of modest (less than 100 V/cm) electric fields, a novel strategy for bionanotubule fabrication with superior throughput and simplicity was developed. Fluorescence and bright field microscopy was used to describe the formation of these bilayer-bound cylindrical structures, which have been previously identified in nature (playing crucial roles in intercellular communication) and made synthetically by direct mechanical manipulation of membranes. In the biological context, the results of this work suggest that mechanical electrostatic interaction may play a role in the shape and function of individual biological membranes and networks of membrane-bound structures. A second project involving liposomes focused on membrane potential measurements in vesicles containing trans-membrane pH gradients. These types of gradients consist of differential charge states in the lipid bilayer leaflets, which have been shown to greatly influence the efficacy of drug targeting and the treatment of diseases such as cancer. Here, these systems are qualitatively and quantitatively assessed by using voltage-sensitive membrane dyes and fluorescence spectroscopy. Bioaerosol studies involved exploring the feasibility of a fingerprinting technology based on current understanding of cellular debris in aerosols and arguments regarding sampling, sensitivity, separations and detection schemes of these debris. Aerosolized particles of cellular material and proteins emitted by humans, animals and plants can be considered information-rich packets that carry biochemical information specific to the living organisms present in the collection settings. These materials could potentially be exploited for identification purposes. Preliminary studies evaluated protein concentration trends in both indoor and outdoor locations. Results indicated that concentrations correlate to certain conditions of the collection environment (e.g. extent of human presence), supporting the idea that bioaerosol fingerprinting is possible.
ContributorsCastillo Gutiérrez, Josemar Andreina (Author) / Hayes, Mark A. (Thesis advisor) / Herckes, Pierre (Committee member) / Ghrilanda, Giovanna (Committee member) / Arizona State University (Publisher)
Created2011
Description
A primary need of Forensic science is to individualize missing persons that cannot be identified after death. With the use of advanced technology, Radio Frequency Identification (RFID) implant chips can drastically improve digital tracking and enable robust biological and legal identification. In this paper, I will discuss applications between different microchip technologies and indicate reasons why the RFID chip is more useful for forensic science. My results state that an RFID chip is significantly more capable of integrating a mass volume of background information, and can utilize implanted individuals’ DNA profiles to decrease the missing persons database backlogs. Since today’s society uses a lot of digital devices that can ultimately identify people by simple posts or geolocation, Forensic Science can harness that data as an advantage to help serve justice for the public in giving loved ones closure.
ContributorsChastain, Hope Natasha (Author) / Kanthswamy, Sree (Thesis director) / Oldt, Robert (Committee member) / School of Mathematical and Natural Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description
The need to draw a more explicit connection between literature and the daily lives of students has become an increasingly pressing issue. Preeminent literary scholars have long argued that the design of many undergraduate classrooms only engages the student with literature to a degree that, long term, does not produce habits of criticism that engage students with wider contexts of conflict. The yield instead primarily takes place in a classroom. Leading scholars tend to draw connections of value between the work they are teaching and the lives of students by focusing on how they negotiate specific power discourses. However, placing an emphasis on having habits of criticism function regarding specific biases in contexts restricts the kinds of conflict students are prepared to negotiate. To encourage a habit of critical thinking in undergraduate students that can be applied to any context of conflict and bias, a vocabulary on language failure should be taught and analyzed through its implications in origin myths that explain and justify division. Language failure, or the failure of symbols to represent subjects in their full capacity, is a concept and theory introduced by Kenneth Burke to examine conflict at a conceivable root. Burke suggests that language failure is the core of misrepresentation and conflict and is inevitably the result of any ‘identification’, or selection of meaning that is assigned to symbols. Identifications are selections of meaning and conceptions of value that organize bodies towards a social purpose, under a limited perspective. The danger of language failure is present when it goes unacknowledged. In identifications, the repercussions of language failure continually complicate, divide and propagate in discourse; assumptions about the validity of identifications encourage more complex ‘blind-spots’ and misrepresentations that exclude populations and have violent potentials. The more complex the layering of association between identifications becomes, the more obscured their foundational failure, their nature of non-innateness, is, faced as truth, affective as justice. The ‘affective’ foundation behind these powerful associations and assumptions is myth. Origin myth, or a narrative that explains the beginning of some worldly phenomena, founds, and adapts to the needs of culture and society. Teaching students to regard risks of language use as being foundational in their cultural thought, their criticism, and their communication, enriches their capability to negotiate and participate ambivalently in conflicts faced during their daily lives.
ContributorsNewcomb, Lily R. (Author) / Jensen, Kyle (Thesis advisor) / Fox, Cora (Committee member) / Free, Melissa (Committee member) / Arizona State University (Publisher)
Created2021